NM_022167.4;PXYLT2; SOS; XT-II; XT2; xylT-II
100μg liquid plasmid(Instantly):$120
| name | pCAGGS-XYLT2(human)-V5(3synonymous mutations) | another name | apCAGGS-XYLT2(human)-V5(3synonymous mutations) |
| Insert Size (bp) | 2595bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CAG | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | pcaggs-F：GTTCGGCTTCTGGCGTGT | |
| 3′ sequencing primer | pcaggs-R：TATGTCCTTCCGAGTGAGAG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTCCTCGAGC CACCATGGTG GCGAGCGCGC GAGTGCAGAA GCTGGTGCGG CGCTACAAGC TGGCGATTGC CACGGCGCTG GCCATCCTGC TGCTGCAGGG CCTGGTAGTG TGGAGCTTCA GCGGCCTGGA GGAGGACGAG GCGGGCGAGA AAGGAAGGCA GAGGAAGCCA CGGCCACTGG ACCCTGGCGA GGGTTCCAAG GACACAGACA GTTCAGCAGG GCGACGGGGC AGCACAGGCA GAAGGCATGG GCGCTGGCGG GGCCGTGCTG AGAGCCCAGG AGTGCCCGTG GCCAAGGTGG TACGGGCAGT AACCAGCCGG CAGAGAGCCA GCCGGCGGGT CCCACCTGCC CCACCCCCGG AAGCCCCAGG CCGCCAGAAC CTGAGTGGGG CAGCAGCTGG GGAGGCGCTG GTAGGGGCAG CTGGCTTCCC ACCACACGGA GATACAGGGA GCGTGGAGGG CGCCCCCCAG CCCACGGACA ATGGCTTCAC CCCCAAGTGC GAGATCGTGG GCAAGGACGC ACTGTCTGCA CTGGCCCGGG CCAGCACCAA GCAGTGCCAG CAGGAGATCG CCAATGTGGT GTGCCTGCAC CAGGCTGGGA GCCTCATGCC CAAGGCTGTG CCCCGGCACT GTCAGCTGAC TGGGAAGATG AGCCCCGGCA TCCAGTGGGA TGAGAGCCAA GCCCAGCAGC CCATGGATGG CCCCCCGGTG CGAATCGCCT ACATGCTGGT GGTTCACGGC CGCGCCATCC GCCAGCTGAA GCGTCTCCTC AAGGCCGTTT ATCACGAGCA GCACTTCTTT TACATCCATG TGGACAAGCG TTCCGACTAC CTGCACCGGG AGGTGGTGGA GCTGGCCCAG GGCTATGATA ACGTGCGGGT GACGCCCTGG CGCATGGTTA CCATCTGGGG CGGGGCCAGC CTCCTGAGGA TGTACCTGCG GAGCATGCGG GACCTGCTAG AGGTGCCTGG CTGGGCCTGG GACTTCTTCA TCAACCTCAG TGCCACTGAC TATCCAACCA GGACCAATGA GGAGCTGGTG GCATTCCTAT CCAAGAACCG GGACAAGAAT TTCCTCAAGT CACATGGCCG GGACAACTCC AGGTTCATCA AGAAACAGGG CCTGGACCGG CTCTTCCATG AGTGCGACTC ACACATGTGG CGCCTGGGCG AGCGGCAGAT CCCAGCAGGC ATTGTGGTGG ATGGCGGTTC TGACTGGTTC GTGCTGACAC GCAGCTTTGT GGAGTATGTG GTGTACACAG ATGACCCGCT TGTGGCCCAG CTGCGCCAGT TCTACACATA CACACTGCTC CCAGCCGAGT CCTTCTTCCA CACGGTGCTG GAGAACAGCC TGGCCTGTGA GACCCTCGTG GACAACAACC TGCGGGTCAC CAACTGGAAC CGCAAGCTGG GCTGCAAGTG CCAGTACAAG CACATTGTGG ACTGGTGTGG CTGCTCCCCC AACGACTTCA AGCCACAGGA CTTCCTCCGG CTGCAGCAAG TCTCCAGACC CACCTTCTTC GCCCGGAAGT TCGAGTCGAC TGTGAACCAG GAGGTGCTGG AAATCCTGGA CTTCCACCTG TATGGCAGCT ACCCCCCCGG CACGCCAGCC CTCAAGGCCT ACTGGGAGAA CACCTACGAC GCGGCTGATG GCCCCAGTGG GCTCAGTGAT GTCATGCTCA CTGCTTACAC AGCCTTCGCC CGCCTCAGCC TGCACCATGC CGCCACTGCT GCACCCCCAA TGGGCACCCC ACTCTGCAGG TTTGAGCCCA GGGGCTTGCC GTCCAGCGTG CACCTGTATT TCTATGACGA CCATTTCCAG GGCTACCTGG TGACGCAGGC GGTGCAGCCC TCAGCCCAGG GGCCGGCAGA GACGCTTGAG ATGTGGCTGA TGCCCCAAGG GTCGCTGAAG CTGTTGGGGC GCAGTGACCA GGCCAGCCGG CTCCAGAGTC TGGAGGTTGG CACTGATTGG GACCCCAAAG AGCGTCTTTT CCGGAACTTT GGGGGGTTAC TGGGGCCGCT GGACGAGCCT GTGGCCGTGC AGCGCTGGGC CCGGGGCCCC AACCTCACAG CCACAGTGGT CTGGATCGAC CCAACCTATG TGGTGGCCAC ATCTTATGAC ATCACAGTAG ATACGGAGAC TGAGGTCACG CAATACAAGC CCCCACTGAG CCGGCCCCTG CGGCCAGGGC CCTGGACTGT TCGACTCCTT CAGTTCTGGG AACCGCTGGG TGAGACCCGC TTCCTTGTGC TGCCCTTGAC CTTCAACCGC AAACTACCTC TCAGGAAAGA TGATGCCAGC TGGCTGCACG CAGGGCCACC CCACAACGAG TACATGGAGC AGAGTTTCCA GGGCCTGAGT AGCATCCTGA ACCTGCCTCA GCCGGAGCTC GCGGAGGAGG CTGCCCAGCG GCACACACAG CTCACAGGCC CTGCGCTCGA GGCCTGGACA GACAGGGAAC TGAGCAGCTT CTGGTCCGTG GCTGGACTGT GTGCCATAGG CCCCTCTCCC TGCCCCTCCC TGGAGCCCTG CAGACTGACC AGCTGGAGCT CTCTGTCCCC CGACCCCAAA TCAGAGCTGG GGCCTGTCAA AGCAGACGGG CGACTCAGGG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGAGATCT TTTTCCCTCT GCCAAAAATT ATGGGGACAT CATGAAGCCC CTTGAGCATC TGACTTCTGG CTAATAAAGG AAATTTATTT TCATTGCAAT AGTGTGTTGG AATTTTTTGT GTCTCTCACT CGGAAGGACA TATGGGAGGG CAAATCATTT AAAACATCAG AATGAGTATT TGGTTTAGAG TTTGGCAACA TATGCCCATA TGCTGGCTGC CATGAACAAA GGTTGGCTAT AAAGAGGTCA TCAGTATATG AAACAGCCCC CTGCTGTCCA TTCCTTATTC CATAGAAAAG CCTTGACTTG AGGTTAGATT TTTTTTATAT TTTGTTTTGT GTTATTTTTT TCTTTAACAT CCCTAAAATT TTCCTTACAT GTTTTACTAG CCAGATTTTT CCTCCTCTCC TGACTACTCC CAGTCATAGC TGTCCCTCTT CTCTTATGGA GATCCCTCGA CCTGCAGCCC AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCGGATCC GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGGGTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT GCTTCACTCT CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT AATTATTTTG TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCCCCCCCCA GGCGGGGCGG GGCGGGGCGA GGGGCGGGGC GGGGCGAGGC GGAAAGGTGC GGCGGCAGCC AATCAGAGCG GCGCGCTCCG AAAGTTTCCT TTTATGGCGA GGCGGCGGCG GCGGCGGCCC TATAAAAAGC GAAGCGCGCG GCGGGCGGGA GTCGTTGCGC GCTGCCTTCC CCCCGTGCCC CGCTCCGCCG CCGCCTCGCG CCGCCCGCCC CGGCTCTGAC TGACCGCGTT ACTCCCACAG GTGAGCGGGC GGGACGGCCC TTCTCCTCCG GGCTGTAATT AGCGCTTGGT TTAATGACGG CTTGTTTCTT TTCTGTGGCT GCGTGAAAGC CTTGAGGGGC TCCGGGAGGG CCCTTTGTGC GGGGGGAGCG GCTCGGGGGG TGCGTGCGTG TGTGTGTGCG TGGGGAGCGC CGCGTGCGGC TCCGCGCTGC CCGGCGGCTG TGAGCGCTGC GGGCGCGGCG CGGGGCTTTG TGCGCTCCGC AGTGTGCGCG AGGGGAGCGC GGCCGGGGGC GGTGCCCCGC GGTGCGGGGG GGGCTGCGAG GGGAACAAAG GCTGCGTGCG GGGTGTGTGC GTGGGGGGGT GAGCAGGGGG TGTGGGCGCG TCGGTCGGGC TGCAACCCCC CCTGCACCCC CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTAC GGGGCGTGGC GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG CCGCCTCGGG CCGGGGAGGG CTCGGGGGAA GGGGCGCGGC GGCCCCCGGA GCGCCGGCGG CTGTCGAGGC GCGGCGAGCC GCAGCCATTG CCTTTTATGG TAATCGTGCG AGAGGGCGCA GGGACTTCCT TTGTCCCAAA TCTGTGCGGA GCCGAAATCT GGGAGGCGCC GCCGCACCCC CTCTAGCGGG CGCGGGGCGA AGCGGTGCGG CGCCGGCAGG AAGGAAATGG GCGGGGAGGG CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCCTCTC CAGCCTCGGG GCTGTCCGCG GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAA
