NM_000719.7;CACH2; CACN2; CACNL1A1; CaV1.2; CCHL1A1
100μg liquid plasmid(Instantly):$192
| name | pCDH-CMV-CACNA1C(human)-EF1-turboRFP-T2A-Puro(1synonymous mutations) | another name | apCDH-CMV-CACNA1C(human)-EF1-turboRFP-T2A-Puro(1synonymous mutations) |
| Insert Size (bp) | 6417bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT AGAGCTAGCG AATTCGCCAC CATGGTCAAT GAGAATACGA GGATGTACAT TCCAGAGGAA AACCACCAAG GTTCCAACTA TGGGAGCCCA CGCCCCGCCC ATGCCAACAT GAATGCCAAT GCGGCAGCGG GGCTGGCCCC TGAGCACATC CCCACCCCGG GGGCTGCCCT GTCGTGGCAG GCGGCCATCG ACGCAGCCCG GCAGGCTAAG CTGATGGGCA GCGCTGGCAA TGCGACCATC TCCACAGTCA GCTCCACGCA GCGGAAGCGG CAGCAATATG GGAAACCCAA GAAGCAGGGC AGCACCACGG CCACACGCCC GCCCCGAGCC CTGCTCTGCC TGACCCTGAA GAACCCCATC CGGAGGGCCT GCATCAGCAT TGTCGAATGG AAACCATTTG AAATAATTAT TTTACTGACT ATTTTTGCCA ATTGTGTGGC CTTAGCGATC TATATTCCCT TTCCAGAAGA TGATTCCAAC GCCACCAATT CCAACCTGGA ACGAGTGGAA TATCTCTTTC TCATAATTTT TACGGTGGAA GCGTTTTTAA AAGTAATCGC CTATGGACTC CTCTTTCACC CCAATGCCTA CCTCCGCAAC GGCTGGAACC TACTAGATTT TATAATTGTG GTTGTGGGGC TTTTTAGTGC AATTTTAGAA CAAGCAACCA AAGCAGATGG GGCAAACGCT CTCGGAGGGA AAGGGGCCGG ATTTGATGTG AAGGCGCTGA GGGCCTTCCG CGTGCTGCGC CCCCTGCGGC TGGTGTCCGG AGTCCCAAGT CTCCAGGTGG TCCTGAATTC CATCATCAAG GCCATGGTCC CCCTGCTGCA CATCGCCCTG CTTGTGCTGT TTGTCATCAT CATCTACGCC ATCATCGGCT TGGAGCTCTT CATGGGGAAG ATGCACAAGA CCTGCTACAA CCAGGAGGGC ATAGCAGATG TTCCAGCAGA AGATGACCCT TCCCCTTGTG CGCTGGAAAC GGGCCACGGG CGGCAGTGCC AGAACGGCAC GGTGTGCAAG CCCGGCTGGG ATGGTCCCAA GCACGGCATC ACCAACTTTG ACAACTTTGC CTTCGCCATG CTCACGGTGT TCCAGTGCAT CACCATGGAG GGCTGGACGG ACGTGCTGTA CTGGGTCAAT GATGCCGTAG GAAGGGACTG GCCCTGGATC TATTTTGTTA CACTAATCAT CATAGGGTCA TTTTTTGTAC TTAACTTGGT TCTCGGTGTG CTTAGCGGAG AGTTTTCCAA AGAGAGGGAG AAGGCCAAGG CCCGGGGAGA TTTCCAGAAG CTGCGGGAGA AGCAGCAGCT AGAAGAGGAT CTCAAAGGCT ACCTGGATTG GATCACTCAG GCCGAAGACA TCGATCCTGA GAATGAGGAC GAAGGCATGG ATGAGGAGAA GCCCCGAAAC ATGAGCATGC CCACCAGTGA GACCGAGTCC GTCAACACCG AAAACGTGGC TGGAGGTGAC ATCGAGGGAG AAAACTGCGG GGCCAGGCTG GCCCACCGGA TCTCCAAGTC AAAGTTCAGC CGCTACTGGC GCCGGTGGAA TCGGTTCTGC AGAAGGAAGT GCCGCGCCGC AGTCAAGTCT AATGTCTTCT ACTGGCTGGT GATTTTCCTG GTGTTCCTCA ACACGCTCAC CATTGCCTCT GAGCACTACA ACCAGCCCAA CTGGCTCACA GAAGTCCAAG ACACGGCAAA CAAGGCCCTG CTGGCCCTGT TCACGGCAGA GATGCTCCTG AAGATGTACA GCCTGGGCCT GCAGGCCTAC TTCGTGTCCC TCTTCAACCG CTTTGACTGC TTCGTCGTGT GTGGCGGCAT CCTGGAGACC ATCCTGGTGG AGACCAAGAT CATGTCCCCA CTGGGCATCT CCGTGCTCAG ATGCGTCCGG CTGCTGAGGA TTTTCAAGAT CACGAGGTAC TGGAACTCCT TGAGCAACCT GGTGGCATCC TTGCTGAACT CTGTGCGCTC CATCGCCTCC CTGCTCCTTC TCCTCTTCCT CTTCATCATC ATCTTCTCCC TCCTGGGGAT GCAGCTCTTT GGAGGAAAGT TCAACTTTGA TGAGATGCAG ACCCGGAGGA GCACATTCGA TAACTTCCCC CAGTCCCTCC TCACTGTGTT TCAGATCCTG ACCGGGGAGG ACTGGAATTC GGTGATGTAT GATGGGATCA TGGCTTATGG CGGCCCCTCT TTTCCAGGGA TGTTAGTCTG TATTTACTTC ATCATCCTCT TCATCTGTGG AAACTATATC CTACTGAATG TGTTCTTGGC CATTGCTGTG GACAACCTGG CTGATGCTGA GAGCCTCACA TCTGCCCAAA AGGAGGAGGA AGAGGAGAAG GAGAGAAAGA AGCTGGCCAG GACTGCCAGC CCAGAGAAGA AACAAGAGTT GGTGGAGAAG CCGGCAGTGG GGGAATCCAA GGAGGAGAAG ATTGAGCTGA AATCCATCAC GGCTGACGGA GAGTCTCCAC CCGCCACCAA GATCAACATG GATGACCTCC AGCCCAATGA AAATGAGGAT AAGAGCCCCT ACCCCAACCC AGAAACTACA GGAGAAGAGG ATGAGGAGGA GCCAGAGATG CCTGTCGGCC CTCGCCCACG ACCACTCTCT GAGCTTCACC TTAAGGAAAA GGCAGTGCCC ATGCCAGAAG CCAGCGCGTT TTTCATCTTC AGCTCTAACA ACAGGTTTCG CCTCCAGTGC CACCGCATTG TCAATGACAC GATCTTCACC AACCTGATCC TCTTCTTCAT TCTGCTCAGC AGCATTTCCC TGGCTGCTGA GGACCCGGTC CAGCACACCT CCTTCAGGAA CCATATTCTG TTTTATTTTG ATATTGTTTT TACCACCATT TTCACCATTG AAATTGCTCT GAAGATGACT GCTTATGGGG CTTTCTTGCA CAAGGGTTCT TTCTGCCGGA ACTACTTCAA CATCCTGGAC CTGCTGGTGG TCAGCGTGTC CCTCATCTCC TTTGGCATCC AGTCCAGTGC AATCAATGTC GTGAAGATCT TGCGAGTCCT GCGAGTACTC AGGCCCCTGA GGGCCATCAA CAGGGCCAAG GGGCTAAAGC ATGTGGTTCA GTGTGTGTTT GTCGCCATCC GGACCATCGG GAACATCGTG ATTGTCACCA CCCTGCTGCA GTTCATGTTT GCCTGCATCG GGGTCCAGCT CTTCAAGGGA AAGCTGTACA CCTGTTCAGA CAGTTCCAAG CAGACAGAGG CGGAATGCAA GGGCAACTAC ATCACGTACA AAGACGGGGA GGTTGACCAC CCCATCATCC AACCCCGCAG CTGGGAGAAC AGCAAGTTTG ACTTTGACAA TGTTCTGGCA GCCATGATGG CCCTCTTCAC CGTCTCCACC TTCGAAGGGT GGCCAGAGCT GCTGTACCGC TCCATCGACT CCCACACGGA AGACAAGGGC CCCATCTACA ACTACCGTGT GGAGATCTCC ATCTTCTTCA TCATCTACAT CATCATCATC GCCTTCTTCA TGATGAACAT CTTCGTGGGC TTCGTCATCG TCACCTTTCA GGAGCAGGGG GAGCAGGAGT ACAAGAACTG TGAGCTGGAC AAGAACCAGC GACAGTGCGT GGAATACGCC CTCAAGGCCC GGCCCCTGCG GAGGTACATC CCCAAGAACC AGCACCAGTA CAAAGTGTGG TACGTGGTCA ACTCCACCTA CTTCGAGTAC CTGATGTTCG TCCTCATCCT GCTCAACACC ATCTGCCTGG CCATGCAGCA CTACGGCCAG AGCTGCCTGT TCAAAATCGC CATGAACATC CTCAACATGC TCTTCACTGG CCTCTTCACC GTGGAGATGA TCCTGAAGCT CATTGCCTTC AAACCCAAGC ACTATTTCTG TGATGCATGG AATACATTTG ACGCCTTGAT TGTTGTGGGT AGCATTGTTG ATATAGCAAT CACCGAGGTA AACCCAGCTG AACATACCCA ATGCTCTCCC TCTATGAACG CAGAGGAAAA CTCCCGCATC TCCATCACCT TCTTCCGCCT GTTCCGGGTC ATGCGTCTGG TGAAGCTGCT GAGCCGTGGG GAGGGCATCC GGACGCTGCT GTGGACCTTC ATCAAGTCCT TCCAGGCCCT GCCCTATGTG GCCCTCCTGA TCGTGATGCT GTTCTTCATC TACGCGGTGA TCGGGATGCA GGTGTTTGGG AAAATTGCCC TGAATGATAC CACAGAGATC AACCGGAACA ACAACTTTCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGTGCC ACCGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CAGGCAAGAA GTGTGCCCCA GAGTCCGAGC CCAGCAACAG CACGGAGGGT GAAACACCCT GTGGTAGCAG CTTTGCTGTC TTCTACTTCA TCAGCTTCTA CATGCTCTGT GCCTTCCTGA TCATCAACCT CTTTGTAGCT GTCATCATGG ACAACTTTGA CTACCTGACA AGGGACTGGT CCATCCTTGG TCCCCACCAC CTGGATGAGT TTAAAAGAAT CTGGGCAGAG TATGACCCTG AAGCCAAGGG TCGTATCAAA CACCTGGATG TGGTGACCCT CCTCCGGCGG ATTCAGCCGC CACTAGGTTT TGGGAAGCTG TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCTCT GAACAGCGAC GGGACAGTCA TGTTCAATGC CACCCTGTTT GCCCTGGTCA GGACGGCCCT GAGGATCAAA ACAGAAGGGA ACCTAGAACA AGCCAATGAG GAGCTGCGGG CGATCATCAA GAAGATCTGG AAGCGGACCA GCATGAAGCT GCTGGACCAG GTGGTGCCCC CTGCAGGTGA TGATGAGGTC ACCGTTGGCA AGTTCTACGC CACGTTCCTG ATCCAGGAGT ACTTCCGGAA GTTCAAGAAG CGCAAAGAGC AGGGCCTTGT GGGCAAGCCC TCCCAGAGGA ACGCGCTGTC TCTGCAGGCT GGCTTGCGCA CACTGCATGA CATCGGGCCT GAGATCCGAC GGGCCATCTC TGGAGATCTC ACCGCTGAGG AGGAGCTGGA CAAGGCCATG AAGGAGGCTG TGTCCGCTGC TTCTGAAGAT GACATCTTCA GGAGGGCCGG TGGCCTGTTC GGCAACCACG TCAGCTACTA CCAAAGCGAC GGCCGGAGCG CCTTCCCCCA GACCTTCACC ACTCAGCGCC CGCTGCACAT CAACAAGGCG GGCAGCAGCC AGGGCGACAC TGAGTCGCCA TCCCACGAGA AGCTGGTGGA CTCCACCTTC ACCCCGAGCA GCTACTCGTC CACCGGCTCC AACGCCAACA TCAACAACGC CAACAACACC GCCCTGGGTC GCCTCCCTCG CCCCGCCGGC TACCCCAGCA CGGTCAGCAC TGTGGAGGGC CACGGGCCCC CCTTGTCCCC TGCCATCCGG GTGCAGGAGG TGGCGTGGAA GCTCAGCTCC AACAGGTGCC ACTCCCGGGA GAGCCAGGCA GCCATGGCGG GTCAGGAGGA GACGTCTCAG GATGAGACCT ATGAAGTGAA GATGAACCAT GACACGGAGG CCTGCAGTGA GCCCAGCCTG CTCTCCACAG AGATGCTCTC CTACCAGGAT GACGAAAATC GGCAACTGAC GCTCCCAGAG GAGGACAAGA GGGACATCCG GCAATCTCCG AAGAGGGGTT TCCTCCGCTC TGCCTCACTA GGTCGAAGGG CCTCCTTCCA CCTGGAATGT CTGAAGCGAC AGAAGGACCG AGGGGGAGAC ATCTCTCAGA AGACAGTCCT GCCCTTGCAT CTGGTTCATC ATCAGGCATT GGCAGTGGCA GGCCTGAGCC CCCTCCTCCA GAGAAGCCAT TCCCCTGCCT CATTCCCTAG GCCTTTTGCC ACCCCACCAG CCACACCTGG CAGCCGAGGC TGGCCCCCAC AGCCCGTCCC CACCCTGCGG CTTGAGGGGG TCGAGTCCAG TGAGAAACTC AACAGCAGCT TCCCATCCAT CCACTGCGGC TCCTGGGCTG AGACCACCCC CGGTGGCGGG GGCAGCAGCG CCGCCCGGAG AGTCCGGCCC GTCTCCCTCA TGGTGCCCAG CCAGGCTGGG GCCCCAGGGA GGCAGTTCCA CGGCAGTGCC AGCAGCCTGG TGGAAGCGGT CTTGATTTCA GAAGGACTGG GGCAGTTTGC TCAAGATCCC AAGTTCATCG AGGTCACCAC CCAGGAGCTG GCCGACGCCT GCGACATGAC CATAGAGGAG ATGGAGAGCG CGGCCGACAA CATCCTCAGC GGGGGCGCCC CACAGAGCCC CAATGGCGCC CTCTTACCCT TTGTGAACTG CAGGGACGCG GGGCAGGACC GAGCCGGGGG CGAAGAGGAC GCGGGCTGTG TGCGCGCGCG GGGTCGACCG AGTGAGGAGG AGCTCCAGGA CAGCAGGGTC TACGTCAGCA GCCTGTAGGG ATCCGCGGCC GCAAGGATCT GCGATCGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACGC TAGACGCCAC CATGAGCGAG CTGATCAAGG AGAACATGCA CATGAAGCTG TACATGGAGG GCACCGTGAA CAACCACCAC TTCAAGTGCA CATCCGAGGG CGAAGGCAAG CCCTACGAGG GCACCCAGAC CATGAAGATC AAGGTGGTCG AGGGCGGCCC TCTCCCCTTC GCCTTCGACA TCCTGGCTAC CAGCTTCATG TACGGCAGCA AAGCCTTCAT CAACCACACC CAGGGCATCC CCGACTTCTT TAAGCAGTCC TTCCCTGAGG GCTTCACATG GGAGAGAATC ACCACATACG AAGACGGGGG CGTGCTGACC GCTACCCAGG ACACCAGCTT CCAGAACGGC TGCATCATCT ACAACGTCAA GATCAACGGG GTGAACTTCC CATCCAACGG CCCTGTGATG CAGAAGAAAA CACGCGGCTG GGAGGCCAAC ACCGAGATGC TGTACCCCGC TGACGGCGGC CTGAGAGGCC ACAGCCAGAT GGCCCTGAAG CTCGTGGGCG GGGGCTACCT GCACTGCTCC TTCAAGACCA CATACAGATC CAAGAAACCC GCTAAGAACC TCAAGATGCC CGGCTTCCAC TTCGTGGACC ACAGACTGGA AAGAATCAAG GAGGCCGACA AAGAGACCTA CGTCGAGCAG CACGAGATGG CTGTGGCCAA GTACTGCGAC CTCCCTAGCA AACTGGGGCA CAGAGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC CCGGCCCTAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAAAT AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC TAGCTATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGACTTTT GCAGAGACGG CCCAAATTCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTG
