pAPOBEC_nCas9_Ung
$113.00
In stock
SKU
PL64170
100μg liquid plasmid(Instantly):$134
| name | pAPOBEC_nCas9_Ung | another name | apAPOBEC_nCas9_Ung |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCCAAGCTAA TTCGAGCTCG GTACCTGACC CGGTCGTGCC CCTCTCTAGA TAAAGGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGTCGACG GATCCATGCC AAAGAAGAAG AGGAAGGTTT CATCGGAGAC CGGCCCTGTT GCTGTTGACC CCACCCTGCG GCGGAGAATC GAGCCACACG AGTTCGAGGT GTTCTTCGAC CCAAGGGAGC TCCGCAAGGA GACGTGCCTC CTGTACGAGA TCAACTGGGG CGGCAGGCAC TCCATCTGGA GGCACACCAG CCAAAACACC AACAAGCACG TGGAGGTCAA CTTCATCGAG AAGTTCACCA CCGAGAGGTA CTTCTGCCCA AACACCCGCT GCTCCATCAC CTGGTTCCTG TCCTGGAGCC CATGCGGCGA GTGCTCCAGG GCCATCACCG AGTTCCTCAG CCGCTACCCA CACGTCACCC TGTTCATCTA CATCGCCAGG CTCTACCACC ACGCCGACCC AAGGAACAGG CAGGGCCTCC GCGACCTGAT CTCCAGCGGC GTGACCATCC AAATCATGAC CGAGCAGGAG TCCGGCTACT GCTGGAGGAA CTTCGTCAAC TACTCCCCAA GCAACGAGGC CCACTGGCCA AGGTACCCAC ACCTCTGGGT GCGCCTCTAC GTGCTCGAGC TGTACTGCAT CATCCTCGGC CTGCCACCAT GCCTCAACAT CCTGAGGCGC AAGCAACCAC AGCTGACCTT CTTCACCATC GCCCTCCAAA GCTGCCACTA CCAGAGGCTC CCACCACACA TCCTGTGGGC TACCGGCCTC AAGTCCGGCA GCGAGACGCC AGGCACCTCC GAGAGCGCTA CGCCTGAACT TAAGGACAAG AAGTACTCGA TCGGCCTCGC CATCGGGACG AACTCAGTTG GCTGGGCCGT GATCACCGAC GAGTACAAGG TGCCCTCTAA GAAGTTCAAG GTCCTGGGGA ACACCGACCG CCATTCCATC AAGAAGAACC TCATCGGCGC TCTCCTGTTC GACAGCGGGG AGACCGCTGA GGCTACGAGG CTCAAGAGAA CCGCTAGGCG CCGGTACACG AGAAGGAAGA ACAGGATCTG CTACCTCCAA GAGATTTTCT CCAACGAGAT GGCCAAGGTT GACGATTCAT TCTTCCACCG CCTGGAGGAG TCTTTCCTCG TGGAGGAGGA TAAGAAGCAC GAGCGGCATC CCATCTTCGG CAACATCGTG GACGAGGTTG CCTACCACGA GAAGTACCCT ACGATCTACC ATCTGCGGAA GAAGCTCGTG GACTCCACCG ATAAGGCGGA CCTCAGACTG ATCTACCTCG CTCTGGCCCA CATGATCAAG TTCCGCGGCC ATTTCCTGAT CGAGGGGGAT CTCAACCCAG ACAACAGCGA TGTTGACAAG CTGTTCATCC AACTCGTGCA GACCTACAAC CAACTCTTCG AGGAGAACCC GATCAACGCC TCTGGCGTGG ACGCGAAGGC TATCCTGTCC GCGAGGCTCT CGAAGTCCAG GAGGCTGGAG AACCTGATCG CTCAGCTCCC AGGCGAGAAG AAGAACGGCC TGTTCGGGAA CCTCATCGCT CTCAGCCTGG GGCTCACCCC GAACTTCAAG TCGAACTTCG ATCTCGCTGA GGACGCCAAG CTGCAACTCT CCAAGGACAC CTACGACGAT GACCTCGATA ACCTCCTGGC CCAGATCGGC GATCAATACG CGGACCTGTT CCTCGCTGCC AAGAACCTGT CGGACGCCAT CCTCCTGTCA GATATCCTCC GCGTGAACAC CGAGATCACG AAGGCTCCAC TCTCTGCCTC CATGATCAAG CGCTACGACG AGCACCATCA GGATCTGACC CTCCTGAAGG CGCTGGTCCG CCAACAGCTC CCGGAGAAGT ACAAGGAGAT TTTCTTCGAT CAGTCGAAGA ACGGCTACGC TGGGTACATC GACGGCGGGG CCTCACAAGA GGAGTTCTAC AAGTTCATCA AGCCAATCCT GGAGAAGATG GACGGCACGG AGGAGCTCCT GGTGAAGCTC AACAGGGAGG ACCTCCTGCG GAAGCAGAGA ACCTTCGATA ACGGCAGCAT CCCCCACCAA ATCCATCTCG GGGAGCTGCA CGCCATCCTG AGAAGGCAAG AGGACTTCTA CCCTTTCCTC AAGGATAACC GGGAGAAGAT CGAGAAGATC CTGACCTTCA GAATCCCATA CTACGTCGGC CCTCTCGCGC GGGGGAACTC AAGATTCGCT TGGATGACCC GCAAGTCTGA GGAGACCATC ACGCCGTGGA ACTTCGAGGA GGTGGTGGAC AAGGGCGCTA GCGCTCAGTC GTTCATCGAG AGGATGACCA ACTTCGACAA GAACCTGCCC AACGAGAAGG TGCTCCCTAA GCACTCGCTC CTGTACGAGT ACTTCACCGT CTACAACGAG CTCACGAAGG TGAAGTACGT CACCGAGGGC ATGCGCAAGC CAGCGTTCCT GTCCGGGGAG CAGAAGAAGG CTATCGTGGA CCTCCTGTTC AAGACCAACC GGAAGGTCAC GGTTAAGCAA CTCAAGGAGG ACTACTTCAA GAAGATCGAG TGCTTCGATT CGGTCGAGAT CAGCGGCGTT GAGGACCGCT TCAACGCCAG CCTCGGGACC TACCACGATC TCCTGAAGAT CATCAAGGAT AAGGACTTCC TGGACAACGA GGAGAACGAG GATATCCTGG AGGACATCGT GCTGACCCTC ACGCTGTTCG AGGACAGGGA GATGATCGAG GAGCGCCTGA AGACGTACGC CCATCTCTTC GATGACAAGG TCATGAAGCA ACTCAAGCGC CGGAGATACA CCGGCTGGGG GAGGCTGTCC CGCAAGCTCA TCAACGGCAT CCGGGACAAG CAGTCCGGGA AGACCATCCT CGACTTCCTC AAGAGCGATG GCTTCGCCAA CAGGAACTTC ATGCAACTGA TCCACGATGA CAGCCTCACC TTCAAGGAGG ATATCCAAAA GGCTCAAGTG AGCGGCCAGG GGGACTCGCT GCACGAGCAT ATCGCGAACC TCGCTGGCTC CCCCGCGATC AAGAAGGGCA TCCTCCAGAC CGTGAAGGTT GTGGACGAGC TCGTGAAGGT CATGGGCCGG CACAAGCCTG AGAACATCGT CATCGAGATG GCCAGAGAGA ACCAAACCAC GCAGAAGGGG CAAAAGAACT CTAGGGAGCG CATGAAGCGC ATCGAGGAGG GCATCAAGGA GCTGGGGTCC CAAATCCTCA AGGAGCACCC AGTGGAGAAC ACCCAACTGC AGAACGAGAA GCTCTACCTG TACTACCTCC AGAACGGCAG GGATATGTAC GTGGACCAAG AGCTGGATAT CAACCGCCTC AGCGATTACG ACGTCGATCA TATCGTTCCC CAGTCTTTCC TGAAGGATGA CTCCATCGAC AACAAGGTCC TCACCAGGTC GGACAAGAAC CGCGGCAAGT CAGATAACGT TCCATCTGAG GAGGTCGTTA AGAAGATGAA GAACTACTGG AGGCAGCTCC TGAACGCCAA GCTGATCACG CAAAGGAAGT TCGACAACCT CACCAAGGCT GAGAGAGGCG GGCTCTCAGA GCTGGACAAG GCCGGCTTCA TCAAGCGGCA GCTGGTCGAG ACCAGACAAA TCACGAAGCA CGTTGCGCAA ATCCTCGACT CTCGGATGAA CACGAAGTAC GATGAGAACG ACAAGCTGAT CAGGGAGGTT AAGGTGATCA CCCTGAAGTC TAAGCTCGTC TCCGACTTCA GGAAGGATTT CCAGTTCTAC AAGGTTCGCG AGATCAACAA CTACCACCAT GCCCATGACG CTTACCTCAA CGCTGTGGTC GGCACCGCTC TGATCAAGAA GTACCCAAAG CTGGAGTCCG AGTTCGTGTA CGGGGACTAC AAGGTTTACG ATGTGCGCAA GATGATCGCC AAGTCGGAGC AAGAGATCGG CAAGGCTACC GCCAAGTACT TCTTCTACTC AAACATCATG AACTTCTTCA AGACCGAGAT CACGCTGGCC AACGGCGAGA TCCGGAAGAG ACCGCTCATC GAGACCAACG GCGAGACGGG GGAGATCGTG TGGGACAAGG GCAGGGATTT CGCGACCGTC CGCAAGGTTC TCTCCATGCC CCAGGTGAAC ATCGTCAAGA AGACCGAGGT CCAAACGGGC GGGTTCTCAA AGGAGTCTAT CCTGCCTAAG CGGAACAGCG ACAAGCTCAT CGCCAGAAAG AAGGACTGGG ACCCAAAGAA GTACGGCGGG TTCGACAGCC CTACCGTGGC CTACTCGGTC CTGGTTGTGG CGAAGGTTGA GAAGGGCAAG TCCAAGAAGC TCAAGAGCGT GAAGGAGCTC CTGGGGATCA CCATCATGGA GAGGTCCAGC TTCGAGAAGA ACCCAATCGA CTTCCTGGAG GCCAAGGGCT ACAAGGAGGT GAAGAAGGAC CTGATCATCA AGCTCCCGAA GTACTCTCTC TTCGAGCTGG AGAACGGCAG GAAGAGAATG CTGGCTTCCG CTGGCGAGCT CCAGAAGGGG AACGAGCTCG CGCTGCCAAG CAAGTACGTG AACTTCCTCT ACCTGGCTTC CCACTACGAG AAGCTCAAGG GCAGCCCGGA GGACAACGAG CAAAAGCAGC TGTTCGTCGA GCAGCACAAG CATTACCTCG ACGAGATCAT CGAGCAAATC TCCGAGTTCA GCAAGCGCGT GATCCTCGCC GACGCGAACC TGGATAAGGT CCTCTCCGCC TACAACAAGC ACCGGGACAA GCCCATCAGA GAGCAAGCGG AGAACATCAT CCATCTCTTC ACCCTGACGA ACCTCGGCGC TCCTGCTGCT TTCAAGTACT TCGACACCAC GATCGATCGG AAGAGATACA CCTCCACGAA GGAGGTCCTG GACGCGACCC TCATCCACCA GTCGATCACC GGCCTGTACG AGACGAGGAT CGACCTCTCA CAACTCGGCG GGGATAAGAG ACCCGCAGCA ACCAAGAAGG CAGGGCAAGC AAAGAAGAAG AAGACGCGTG ACTCCGGCGG CAGCATGTTT GGAGAGAGCT GGAAGAAGCA CCTCAGCGGG GAGTTCGGGA AACCGTATTT TATCAAGCTA ATGGGATTTG TTGCAGAAGA AAGAAAGCAT TACACTGTTT ATCCACCCCC ACACCAAGTC TTCACCTGGA CCCAGATGTG TGACATAAAA GATGTGAAGG TTGTCATCCT GGGACAGGAT CCATATCATG GACCTAATCA AGCTCACGGG CTCTGCTTTA GTGTTCAAAG GCCTGTTCCG CCTCCGCCCA GTTTGGAGAA CATTTATAAA GAGTTGTCTA CAGACATAGA GGATTTTGTT CATCCTGGCC ATGGAGATTT ATCTGGGTGG GCCAAGCAAG GTGTTCTCCT TCTCAACGCT GTCCTCACGG TTCGTGCCCA TCAAGCCAAC TCTCATAAGG AGCGAGGCTG GGAGCAGTTC ACTGATGCAG TTGTGTCCTG GCTAAATCAG AACTCGAATG GCCTTGTTTT CTTGCTCTGG GGCTCTTATG CTCAGAAGAA GGGCAGTGCC ATTGATAGGA AGCGGCACCA TGTACTACAG ACGGCTCATC CCTCCCCTTT GTCAGTGTAT AGAGGGTTCT TTGGATGTAG ACACTTTTCA AAGACCAATG AGCTGCTGCA GAAGTCTGGC AAGAAGCCCA TTGACTGGAA GGAGCTGTCG GGGGGGAGCC CAAAGAAGAA GCGGAAGGTG TAGTGTACAA GTAACCCGGG AATTCGGTAC GCTGAAATCA CCAGTCTCTC TCTACAAATC TATCTCTCTC TATTTTCTCC ATAAATAATG TGTGAGTAGT TTCCCGATAA GGGAAATTAG GGTTCTTATA GGGTTTCGCT CATGTGTTGA GCATATAAGA AACCCTTAGT ATGTATTTGT ATTTGTAAAA TACTTCTATC AATAAAATTT CTAATTCCTA AAACCAAAAT CCAGTACTAA AATCCAGATC TCCTAAAGTC CCTATAGATC TTTGTCGTGA ATATAAACCA GACACGAGAC GACTAAACCT GGAGCCCAGA CGCCGTTCGA AGCTAGAAGT ACCGCTTAGG CAGGAGGCCG TTAGGGAAAA GATGCTAAGG CAGGGTTGGT TACGTTGACT CCCCCGTAGG TTTGGTTTAA ATATGATGAA GTGGACGGAA GGAAGGAGGA AGACAAGGAA GGATAAGGTT GCAGGCCCTG TGCAAGGTAA GAAGATGGAA ATTTGATAGA GGTACGCTAC TATACTTATA CTATACGCTA AGGGAATGCT TGTATTTATA CCCTATACCC CCTAATAACC CCTTATCAAT TTAAGAAATA ATCCGCATAA GCCCCCGCTT AAAAATTGGT ATCAGAGCCA TGAATAGGTC TATGACCAAA ACTCAAGAGG ATAAAACCTC ACCAAAATAC GAAAGAGTTC TTAACTCTAA AGATAAAAGA TCTTTCAAGA TCAAAACTAG TTCCCTCACA CCGGTGACGG GGATCGCATG CGATATCTCG AGATCTAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGCCAG T
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