pAAVS1-NDi-TRE3G-c-Myc(human)
$99.00
In stock
SKU
PL64028
100μg liquid plasmid(Instantly):$120
| name | pAAVS1-NDi-TRE3G-c-Myc(human) | another name | apAAVS1-NDi-TRE3G-c-Myc(human) |
| Insert Size (bp) | 1320bp | Plasmid host | Mammary cell |
| Plasmid uses | Gene knock-in | Fragment type | ORF,CRISPR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | TRE3G | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAGGGCGAAT TCAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCGA ATTCGCCCTT AGAGCAGAGC CAGGAACCCC TGTAGGGAAG GGGCAGGAGA GCCAGGGGCA TGAGATGGTG GACGAGGAAG GGGGACAGGG AAGCCTGAGC GCCTCTCCTG GGCTTGCCAA GGACTCAAAC CCAGAAGCCC AGAGCAGGGC CTTAGGGAAG CGGGACCCTG CTCTGGGCGG AGGAATATGT CCCAGATAGC ACTGGGGACT CTTTAAGGAA AGAAGGATGG AGAAAGAGAA AGGGAGTAGA GGCGGCCACG ACCTGGTGAA CACCTAGGAC GCACCATTCT CACAAAGGGA GTTTTCCACA CGGACACCCC CCTCCTCACC ACAGCCCTGC CAGGACGGGG CTGGCTACTG GCCTTATCTC ACAGGTAAAA CTGACGCACG GAGGAACAAT ATAAATTGGG GACTAGAAAG GTGAAGAGCC AAAGTTAGAA CTCAGGACCA ACTTATTCTG ATTTTGTTTT TCCAAACTGC TTCTCCTCTT GGGAAGTGTA AGGAAGCTGC AGCACCAGGA TCAGTGAAAC GCACCAGACA GCCGCGTCAG AGCAGCTCAG GTTCTGGGAG AGGGTAGCGC AGGGTGGCCA CTGAGAACCG GGCAGGTCAC GCATCCCCCC CTTCCCTCCC ACCCCCTGCC AAGCTCTCCC TCCCAGGATC CTCTCTGGCT CCATCGTAAG CAAACCTTAG AGGTTCTGGC AAGGAGAGAG ATGGCTCCAG GAAATGGGGG TGTGTCACCA GATAAGGAAT CTGCCTAACA GGAGGTGGGG GTTAGACCCA ATATCAGGAG ACTAGGAAGG AGGAGGCCTA AGGATGGGGC TTTTCTGTCA CCAATCCTGT CCCTAGTAAA GCTGATATCT CCACCCCACA GTGGTTTACT CCCTATCAGT GATAGAGAAC GTATGAAGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GCAGACTTTA CTCCCTATCA GTGATAGAGA ACGTATAAGG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGACCAGTT TACTCCCTAT CAGTGATAGA GAACGTATCT ACAGTTTACT CCCTATCAGT GATAGAGAAC GTATATCCAG TTTACTCCCT ATCAGTGATA GAGAACGTAT AAGCTTTAGG CGTGTACGGT GGGCGCCTAT AAAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GCAATTCCAC AACACTTTTG TCTTATACCA ACTTTCCGTA CCACTTCCTA CCCTCGTAAA CTTAAGCCAC CATGCCCCTC AACGTTAGCT TCACCAACAG GAACTATGAC CTCGACTACG ACTCGGTGCA GCCGTATTTC TACTGCGACG AGGAGGAGAA CTTCTACCAG CAGCAGCAGC AGAGCGAGCT GCAGCCCCCG GCGCCCAGCG AGGATATCTG GAAGAAATTC GAGCTGCTGC CCACCCCGCC CCTGTCCCCT AGCCGCCGCT CCGGGCTCTG CTCGCCCTCC TACGTTGCGG TCACACCCTT CTCCCTTCGG GGAGACAACG ACGGCGGTGG CGGGAGCTTC TCCACGGCCG ACCAGCTGGA GATGGTGACC GAGCTGCTGG GAGGAGACAT GGTGAACCAG AGTTTCATCT GCGACCCGGA CGACGAGACC TTCATCAAAA ACATCATCAT CCAGGACTGT ATGTGGAGCG GCTTCTCGGC CGCCGCCAAG CTCGTCTCAG AGAAGCTGGC CTCCTACCAG GCTGCGCGCA AAGACAGCGG CAGCCCGAAC CCCGCCCGCG GCCACAGCGT CTGCTCCACC TCCAGCTTGT ACCTGCAGGA TCTGAGCGCC GCCGCCTCAG AGTGCATCGA CCCCTCGGTG GTCTTCCCCT ACCCTCTCAA CGACAGCAGC TCGCCCAAGT CCTGCGCCTC GCAAGACTCC AGCGCCTTCT CTCCGTCCTC GGATTCTCTG CTCTCCTCGA CGGAGTCCTC CCCGCAGGGC AGCCCCGAGC CCCTGGTGCT CCATGAGGAG ACACCGCCCA CCACCAGCAG CGACTCTGAG GAGGAACAAG AAGATGAGGA AGAAATCGAT GTTGTTTCTG TGGAAAAGAG GCAGGCTCCT GGCAAAAGGT CAGAGTCTGG ATCACCTTCT GCTGGAGGCC ACAGCAAACC TCCTCACAGC CCACTGGTCC TCAAGAGGTG CCACGTCTCC ACACATCAGC ACAACTACGC AGCGCCTCCC TCCACTCGGA AGGACTATCC TGCTGCCAAG AGGGTCAAGT TGGACAGTGT CAGAGTCCTG AGACAGATCA GCAACAACCG AAAATGCACC AGCCCCAGGT CCTCGGACAC CGAGGAGAAT GTCAAGAGGC GAACACACAA CGTCTTGGAG CGCCAGAGGA GGAACGAGCT AAAACGGAGC TTTTTTGCCC TGCGTGACCA GATCCCGGAG TTGGAAAACA ATGAAAAGGC CCCCAAGGTA GTTATCCTTA AAAAAGCCAC AGCATACATC CTGTCCGTCC AAGCAGAGGA GCAAAAGCTC ATTTCTGAAG AGGACTTGTT GCGGAAACGA CGAGAACAGT TGAAACACAA ACTTGAACAG CTACGGAACT CTTGTGCGTA AGCGGCCGCA ATCGATCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGACGGCCGC ATAACTTCGT ATAATGTATG CTATACGAAG TTATAAGCTT GGGCTGCAGG TCGAGGGATC TCCATAAGAG AAGAGGGACA GCTATGACTG GGAGTAGTCA GGAGAGGAGG AAAAATCTGG CTAGTAAAAC ATGTAAGGAA AATTTTAGGG ATGTTAAAGA AAAAAATAAC ACAAAACAAA ATATAAAAAA AATCTAACCT CAAGTCAAGG CTTTTCTATG GAATAAGGAA TGGACAGCAG GGGGCTGTTT CATATACTGA TGACCTCTTT ATAGCCAACC TTTGTTCATG GCAGCCAGCA TATGGGCATA TGTTGCCAAA CTCTAAACCA AATACTCATT CTGATGTTTT AAATGATTTG CCCTCCCATA TGTCCTTCCG AGTGAGAGAC ACAAAAAATT CCAACACACT ATTGCAATGA AAATAAATTT CCTTTATTAG CCAGAAGTCA GATGCTCAAG GGGCTTCATG ATGTCCCCAT AATTTTTGGC AGAGGGAAAA AGATCTCAGT GGTATTTGTG AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT GAATTCACGC GTTTAGCCTG GCAGCATATC GAGATCGAAG TCATCGAGAG CGTCAGCAGG GAGCATATCC AGATCAAAAT CATCCAGGGC ATCGGCGGGC AGCATGTCCA GGTCGAAGTC GTCCAGGGCG TCGGTGGGTC CGCCGCTCTC GCACTTCAGC TGCTTCTCGA GGCCGCAGAT GATCAGTTCC AGGCCGAACA GGAAGGCGGG CTCGGCGCCC TGTCTGTCGA ACAGCTCGAT GGCCTGCTTC AGCAGGGGGG GCATGCTGTC GGTGGTGGGT GTCTCTCTTT CCTCTTTGGC GACCTGGTGT TCCTGTTCTT CCAGCACGCA GCCCAGGGTG AAGTGGCCCA CGGCGCTCAG GGCGTACAGG GCGTTTTCCA GGCTGAAGCC CTGCTGGCAC AGGAAGGCCA GCTGGTTTTC CAGTGTCTCG TACTGCTTCT CGGTGGGTCT GGTGCCCAGG TGCACTTTGG CGCCGTCCCG GTGGCTCAGC AGGGCGCATC TGTAGCTCTT GGCGTTGTTC CGCAGGAAGT CCTGCCAGCT CTCGCCTTCC AGAGGGCAGC TGTGGGTGTG GTGCCGGTCC AGCATCTCGA TGGGCAGGGC GTCCAGCAGG GCCCGCTTGT TCTTCACGTG CCAGTACAGG GTGGGCTGTT CCACGCCCAG TTTCTGGGCC AGCTTCCGGG TGGTCAGGCC CTCGATGCCC ACGCCGTTCA GCAGTTCCAG GGCGCTGTTG ATCACTTTGC TCTTGTCCAG CCGGCTCATG TCGACATTCT TTGCCAAAAT GATGAGACAG CACAATAACC AGCACGTTGC CCAGGAGCTG TAGGAAAAAG AAGAAGGCAT GAACATGGTT AGCAGAGGCT CTAGAGCCGC CGGTCACACG CCAGAAGCCG AACCCCGCCC TGCCCCGTCC CCCCCGAAGG CAGCCGTCCC CCCGCGGACA GCCCCGAGGC TGGAGAGGGA GAAGGGGACG GCGGCGCGGC GACGCACGAA GGCCCTCCCC GCCCATTTCC TTCCTGCCGG CGCCGCACCG CTTCGCCCCG CGCCCGCTAG AGGGGGTGCG GCGGCGCCTC CCAGATTTCG GCTCCGCACA GATTTGGGAC AAAGGAAGTC CCTGCGCCCT CTCGCACGAT TACCATAAAA GGCAATGGCT GCGGCTCGCC GCGCCTCGAC AGCCGCCGGC GCTCCGGGGG CCGCCGCGCC CCTCCCCCGA GCCCTCCCCG GCCCGAGGCG GCCCCGCCCC GCCCGGCACC CCCACCTGCC GCCACCCCCC GCCCGGCACG GCGAGCCCCG CGCCACGCCC CGTACGGAGC CCCGCACCCG AAGCCGGGCC GTGCTCAGCA ACTCGGGGAG GGGGGTGCAG GGGGGGTTGC AGCCCGACCG ACGCGCCCAC ACCCCCTGCT CACCCCCCCA CGCACACACC CCGCACGCAG CCTTTGTTCC CCTCGCAGCC CCCCCCGCAC CGCGGGGCAC CGCCCCCGGC CGCGCTCCCC TCGCGCACAC TGCGGAGCGC ACAAAGCCCC GCGCCGCGCC CGCAGCGCTC ACAGCCGCCG GGCAGCGCGG AGCCGCACGC GGCGCTCCCC ACGCACACAC ACACGCACGC ACCCCCCGAG CCGCTCCCCC CGCACAAAGG GCCCTCCCGG AGCCCCTCAA GGCTTTCACG CAGCCACAGA AAAGAAACAA GCCGTCATTA AACCAAGCGC TAATTACAGC CCGGAGGAGA AGGGCCGTCC CGCCCGCTCA CCTGTGGGAG TAACGCGGTC AGTCAGAGCC GGGGCGGGCG GCGCGAGGCG GCGGCGGAGC GGGGCACGGG GCGAAGGCAG CGTCGCAGCG ACTCCCCGCC CGCCGCGCGC TTCGCTTTTT ATAGGGCCGC CGCCGCCGCC GCCTCGCCAT AAAAGGAAAC TTTCGGAGCG CGCCGCTCTG ATTGGCTGCC GCCGCACCTC TCCGCCTCGC CCCGCCCCGC CCCTCGCCCC GCCCCGCCCC GCCTGGCGCG CGCCCCCCCC CCCCCCCCGC CCCCATCGCT GCACAAAATA ATTAAAAAAT AAATAAATAC AAAATTGGGG GTGGGGAGGG GGGGGAGATG GGGAGAGTGA AGCAGAACGT GGGGCTCACC TCGACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTCCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGGCGTTA CTATGGGAAC ATACGTCATT ATTGACGTCA ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT TACCGTAAGT TATGTAACGC GGAACTCCAT ATATGGGCTA TGAACTAATG ACCCCGTAAT TGATTACTAT TAATAACTAG TCAATAATCA ATGTCGAGAC CCATAGAGCC CACCGCATCC CCAGCATGCC TGCTATTGTC TTCCCAATCC TCCCCCTTGC TGTCCTGCCC CACCCCACCC CCCAGAATAG AATGACACCT ACTCAGACAA TGCGATGCAA TTTCCTCATT TTATTAGGAA AGGACAGTGG GAGTGGCACC TTCCAGGGTC AAGGAAGGCA CGGGGGAGGG GCAAACAACA GATGGCTGGC AACTAGAAGG CACAGTCGAG GCTGATCAGC GGGTTTAAAC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGGTC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCA TCGCCGTCGG GCATGCGCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCCTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATGGCCGATC CCATGGTGGC GGTACCCTCG AGCCTAGGGC CGGGATTCTC CTCCACGTCA CCGCATGTTA GAAGACTTCC TCTGCCCTCT CCGCTGCCAG ATCTCTCGAG GCCCTGTGGG AGGAAGAGAA GAGGTCAGAA GCTTATAACT TCGTATAATG TATGCTATAC GAAGTTATTG CCCCACTGTG GGGTGGAGGG GACAGATAAA AGTACCCAGA ACCAGAGCCA CATTAACCGG CCCTGGGAAT ATAAGGTGGT CCCAGCTCGG GGACACAGGA TCCCTGGAGG CAGCAAACAT GCTGTCCTGA AGTGGACATA GGGGCCCGGG TTGGAGGAAG AAGACTAGCT GAGCTCTCGG ACCCCTGGAA GATGCCATGA CAGGGGGCTG GAAGAGCTAG CACAGACTAG AGAGGTAAGG GGGGTAGGGG AGCTGCCCAA ATGAAAGGAG TGAGAGGTGA CCCGAATCCA CAGGAGAACG GGGTGTCCAG GCAAAGAAAG CAAGAGGATG GAGAGGTGGC TAAAGCCAGG GAGACGGGGT ACTTTGGGGT TGTCCAGAAA AACGGTGATG ATGCAGGCCT ACAAGAAGGG GAGGCGGGAC GCAAGGGAGA CATCCGTCGG AGAAGGCCAT CCTAAGAAAC GAGAGATGGC ACAGGCCCCA GAAGGAGAAG GAAAAGGGAA CCCAGCGAGT GAAGACGGCA TGGGGTTGGG TGAGGGAGGA GAGATGCCCG GAGAGGACCC AGACACGGGG AGGATCCGCT CAGAGGACAT CACGTGGTGC AGCGCCGAGA AGGAAGTGCT CCGGAAAGAG CATCCTTGGG CAGCAACACA GCAGAGAGCA AGGGGAAGAG GGAGTGGAGG AAGACGGAAC CTGAAGGAGG CGGCAGGGAA GGATCTGGGC CAGCCGTAGA GGTGACCCAG GCCACAAGCT GCAGACAGAA AGCGGCACAG GCCCAGGGGA GAGAATGCTG GTCAGAGAAA GCA
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