pAAVS1-NDi-TRE3G-PIK3CA(human)
$128.00
In stock
SKU
PL64015
100μg liquid plasmid(Instantly):$149
| name | pAAVS1-NDi-TRE3G-PIK3CA(human) | another name | apAAVS1-NDi-TRE3G-PIK3CA(human) |
| Insert Size (bp) | 3207bp | Plasmid host | Mammary cell |
| Plasmid uses | Gene knock-in | Fragment type | ORF,CRISPR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | TRE3G | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAGGGCGAAT TCAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCGA ATTCGCCCTT AGAGCAGAGC CAGGAACCCC TGTAGGGAAG GGGCAGGAGA GCCAGGGGCA TGAGATGGTG GACGAGGAAG GGGGACAGGG AAGCCTGAGC GCCTCTCCTG GGCTTGCCAA GGACTCAAAC CCAGAAGCCC AGAGCAGGGC CTTAGGGAAG CGGGACCCTG CTCTGGGCGG AGGAATATGT CCCAGATAGC ACTGGGGACT CTTTAAGGAA AGAAGGATGG AGAAAGAGAA AGGGAGTAGA GGCGGCCACG ACCTGGTGAA CACCTAGGAC GCACCATTCT CACAAAGGGA GTTTTCCACA CGGACACCCC CCTCCTCACC ACAGCCCTGC CAGGACGGGG CTGGCTACTG GCCTTATCTC ACAGGTAAAA CTGACGCACG GAGGAACAAT ATAAATTGGG GACTAGAAAG GTGAAGAGCC AAAGTTAGAA CTCAGGACCA ACTTATTCTG ATTTTGTTTT TCCAAACTGC TTCTCCTCTT GGGAAGTGTA AGGAAGCTGC AGCACCAGGA TCAGTGAAAC GCACCAGACA GCCGCGTCAG AGCAGCTCAG GTTCTGGGAG AGGGTAGCGC AGGGTGGCCA CTGAGAACCG GGCAGGTCAC GCATCCCCCC CTTCCCTCCC ACCCCCTGCC AAGCTCTCCC TCCCAGGATC CTCTCTGGCT CCATCGTAAG CAAACCTTAG AGGTTCTGGC AAGGAGAGAG ATGGCTCCAG GAAATGGGGG TGTGTCACCA GATAAGGAAT CTGCCTAACA GGAGGTGGGG GTTAGACCCA ATATCAGGAG ACTAGGAAGG AGGAGGCCTA AGGATGGGGC TTTTCTGTCA CCAATCCTGT CCCTAGTAAA GCTGATATCT CCACCCCACA GTGGTTTACT CCCTATCAGT GATAGAGAAC GTATGAAGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GCAGACTTTA CTCCCTATCA GTGATAGAGA ACGTATAAGG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGACCAGTT TACTCCCTAT CAGTGATAGA GAACGTATCT ACAGTTTACT CCCTATCAGT GATAGAGAAC GTATATCCAG TTTACTCCCT ATCAGTGATA GAGAACGTAT AAGCTTTAGG CGTGTACGGT GGGCGCCTAT AAAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GCAATTCCAC AACACTTTTG TCTTATACCA ACTTTCCGTA CCACTTCCTA CCCTCGTAAA CTTAAGCCAC CATGCCTCCA CGACCATCAT CAGGTGAACT GTGGGGCATC CACTTGATGC CCCCAAGAAT CCTAGTAGAA TGTTTACTAC CAAATGGAAT GATAGTGACT TTAGAATGCC TCCGTGAGGC TACATTAATA ACCATAAAGC ATGAACTATT TAAAGAAGCA AGAAAATACC CCCTCCATCA ACTTCTTCAA GATGAATCTT CTTACATTTT CGTAAGTGTT ACTCAAGAAG CAGAAAGGGA AGAATTTTTT GATGAAACAA GACGACTTTG TGACCTTCGG CTTTTTCAAC CCTTTTTAAA AGTAATTGAA CCAGTAGGCA ACCGTGAAGA AAAGATCCTC AATCGAGAAA TTGGTTTTGC TATCGGCATG CCAGTGTGTG AATTTGATAT GGTTAAAGAT CCAGAAGTAC AGGACTTCCG AAGAAATATT CTGAACGTTT GTAAAGAAGC TGTGGATCTT AGGGACCTCA ATTCACCTCA TAGTAGAGCA ATGTATGTCT ATCCTCCAAA TGTAGAATCT TCACCAGAAT TGCCAAAGCA CATATATAAT AAATTAGATA AAGGGCAAAT AATAGTGGTG ATCTGGGTAA TAGTTTCTCC AAATAATGAC AAGCAGAAGT ATACTCTGAA AATCAACCAT GACTGTGTAC CAGAACAAGT AATTGCTGAA GCAATCAGGA AAAAAACTCG AAGTATGTTG CTATCCTCTG AACAACTAAA ACTCTGTGTT TTAGAATATC AGGGCAAGTA TATTTTAAAA GTGTGTGGAT GTGATGAATA CTTCCTAGAA AAATATCCTC TGAGTCAGTA TAAGTATATA AGAAGCTGTA TAATGCTTGG GAGGATGCCC AATTTGATGT TGATGGCTAA AGAAAGCCTT TATTCTCAAC TGCCAATGGA CTGTTTTACA ATGCCATCTT ATTCCAGACG CATTTCCACA GCTACACCAT ATATGAATGG AGAAACATCT ACAAAATCCC TTTGGGTTAT AAATAGTGCA CTCAGAATAA AAATTCTTTG TGCAACCTAC GTGAATGTAA ATATTCGAGA CATTGATAAG ATCTATGTTC GAACAGGTAT CTACCATGGA GGAGAACCCT TATGTGACAA TGTGAACACT CAAAGAGTAC CTTGTTCCAA TCCCAGGTGG AATGAATGGC TGAATTATGA TATATACATT CCTGATCTTC CTCGTGCTGC TCGACTTTGC CTTTCCATTT GCTCTGTTAA AGGCCGAAAG GGTGCTAAAG AGGAACACTG TCCATTGGCA TGGGGAAATA TAAACTTGTT TGATTACACA GACACTCTAG TATCTGGAAA AATGGCTTTG AATCTTTGGC CAGTACCTCA TGGATTAGAA GATTTGCTGA ACCCTATTGG TGTTACTGGA TCAAATCCAA ATAAAGAAAC TCCATGCTTA GAGTTGGAGT TTGACTGGTT CAGCAGTGTG GTAAAGTTCC CAGATATGTC AGTGATTGAA GAGCATGCCA ATTGGTCTGT ATCCCGAGAA GCAGGATTTA GCTATTCCCA CGCAGGACTG AGTAACAGAC TAGCTAGAGA CAATGAATTA AGGGAAAATG ACAAAGAACA GCTCAAAGCA ATTTCTACAC GAGATCCTCT CTCTGAAATC ACTGAGCAGG AGAAAGATTT TCTATGGAGT CACAGACACT ATTGTGTAAC TATCCCCGAA ATTCTACCCA AATTGCTTCT GTCTGTTAAA TGGAATTCTA GAGATGAAGT AGCCCAGATG TATTGCTTGG TAAAAGATTG GCCTCCAATC AAACCTGAAC AGGCTATGGA ACTTCTGGAC TGTAATTACC CAGATCCTAT GGTTCGAGGT TTTGCTGTTC GGTGCTTGGA AAAATATTTA ACAGATGACA AACTTTCTCA GTATTTAATT CAGCTAGTAC AGGTCCTAAA ATATGAACAA TATTTGGATA ACTTGCTTGT GAGATTTTTA CTGAAGAAAG CATTGACTAA TCAAAGGATT GGGCACTTTT TCTTTTGGCA TTTAAAATCT GAGATGCACA ATAAAACAGT TAGCCAGAGG TTTGGCCTGC TTTTGGAGTC CTATTGTCGT GCATGTGGGA TGTATTTGAA GCACCTGAAT AGGCAAGTCG AGGCAATGGA AAAGCTCATT AACTTAACTG ACATTCTCAA ACAGGAGAAG AAGGATGAAA CACAAAAGGT ACAGATGAAG TTTTTAGTTG AGCAAATGAG GCGACCAGAT TTCATGGATG CTCTACAGGG CTTTCTGTCT CCTCTAAACC CTGCTCATCA ACTAGGAAAC CTCAGGCTTG AAGAGTGTCG AATTATGTCC TCTGCAAAAA GGCCACTGTG GTTGAATTGG GAGAACCCAG ACATCATGTC AGAGTTACTG TTTCAGAACA ATGAGATCAT CTTTAAAAAT GGGGATGATT TACGGCAAGA TATGCTAACA CTTCAAATTA TTCGTATTAT GGAAAATATC TGGCAAAATC AAGGTCTTGA TCTTCGAATG TTACCTTATG GTTGTCTGTC AATCGGTGAC TGTGTGGGAC TTATTGAGGT GGTGCGAAAT TCTCACACTA TTATGCAAAT TCAGTGCAAA GGCGGCTTGA AAGGTGCACT GCAGTTCAAC AGCCACACAC TACATCAGTG GCTCAAAGAC AAGAACAAAG GAGAAATATA TGATGCAGCC ATTGACCTGT TTACACGTTC ATGTGCTGGA TACTGTGTAG CTACCTTCAT TTTGGGAATT GGAGATCGTC ACAATAGTAA CATCATGGTG AAAGACGATG GACAACTGTT TCATATAGAT TTTGGACACT TTTTGGATCA CAAGAAGAAA AAATTTGGTT ATAAACGAGA ACGTGTGCCA TTTGTTTTGA CACAGGATTT CTTAATAGTG ATTAGTAAAG GAGCCCAAGA ATGCACAAAG ACAAGAGAAT TTGAGAGGTT TCAGGAGATG TGTTACAAGG CTTATCTAGC TATTCGACAG CATGCCAATC TCTTCATAAA TCTTTTCTCA ATGATGCTTG GCTCTGGAAT GCCAGAACTA CAATCTTTTG ATGACATTGC ATACATTCGA AAGACCCTAG CCTTAGATAA AACTGAGCAA GAGGCTTTGG AGTATTTCAT GAAACAAATG AATGATGCAC ATCATGGTGG CTGGACAACA AAAATGGATT GGATCTTCCA CACAATTAAA CAGCATGCAT TGAACTGAGC GGCCGCAATC GATCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGA CGGCCGCATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TAAGCTTGGG CTGCAGGTCG AGGGATCTCC ATAAGAGAAG AGGGACAGCT ATGACTGGGA GTAGTCAGGA GAGGAGGAAA AATCTGGCTA GTAAAACATG TAAGGAAAAT TTTAGGGATG TTAAAGAAAA AAATAACACA AAACAAAATA TAAAAAAAAT CTAACCTCAA GTCAAGGCTT TTCTATGGAA TAAGGAATGG ACAGCAGGGG GCTGTTTCAT ATACTGATGA CCTCTTTATA GCCAACCTTT GTTCATGGCA GCCAGCATAT GGGCATATGT TGCCAAACTC TAAACCAAAT ACTCATTCTG ATGTTTTAAA TGATTTGCCC TCCCATATGT CCTTCCGAGT GAGAGACACA AAAAATTCCA ACACACTATT GCAATGAAAA TAAATTTCCT TTATTAGCCA GAAGTCAGAT GCTCAAGGGG CTTCATGATG TCCCCATAAT TTTTGGCAGA GGGAAAAAGA TCTCAGTGGT ATTTGTGAGC CAGGGCATTG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT GAGGAGTGAA TTCACGCGTT TAGCCTGGCA GCATATCGAG ATCGAAGTCA TCGAGAGCGT CAGCAGGGAG CATATCCAGA TCAAAATCAT CCAGGGCATC GGCGGGCAGC ATGTCCAGGT CGAAGTCGTC CAGGGCGTCG GTGGGTCCGC CGCTCTCGCA CTTCAGCTGC TTCTCGAGGC CGCAGATGAT CAGTTCCAGG CCGAACAGGA AGGCGGGCTC GGCGCCCTGT CTGTCGAACA GCTCGATGGC CTGCTTCAGC AGGGGGGGCA TGCTGTCGGT GGTGGGTGTC TCTCTTTCCT CTTTGGCGAC CTGGTGTTCC TGTTCTTCCA GCACGCAGCC CAGGGTGAAG TGGCCCACGG CGCTCAGGGC GTACAGGGCG TTTTCCAGGC TGAAGCCCTG CTGGCACAGG AAGGCCAGCT GGTTTTCCAG TGTCTCGTAC TGCTTCTCGG TGGGTCTGGT GCCCAGGTGC ACTTTGGCGC CGTCCCGGTG GCTCAGCAGG GCGCATCTGT AGCTCTTGGC GTTGTTCCGC AGGAAGTCCT GCCAGCTCTC GCCTTCCAGA GGGCAGCTGT GGGTGTGGTG CCGGTCCAGC ATCTCGATGG GCAGGGCGTC CAGCAGGGCC CGCTTGTTCT TCACGTGCCA GTACAGGGTG GGCTGTTCCA CGCCCAGTTT CTGGGCCAGC TTCCGGGTGG TCAGGCCCTC GATGCCCACG CCGTTCAGCA GTTCCAGGGC GCTGTTGATC ACTTTGCTCT TGTCCAGCCG GCTCATGTCG ACATTCTTTG CCAAAATGAT GAGACAGCAC AATAACCAGC ACGTTGCCCA GGAGCTGTAG GAAAAAGAAG AAGGCATGAA CATGGTTAGC AGAGGCTCTA GAGCCGCCGG TCACACGCCA GAAGCCGAAC CCCGCCCTGC CCCGTCCCCC CCGAAGGCAG CCGTCCCCCC GCGGACAGCC CCGAGGCTGG AGAGGGAGAA GGGGACGGCG GCGCGGCGAC GCACGAAGGC CCTCCCCGCC CATTTCCTTC CTGCCGGCGC CGCACCGCTT CGCCCCGCGC CCGCTAGAGG GGGTGCGGCG GCGCCTCCCA GATTTCGGCT CCGCACAGAT TTGGGACAAA GGAAGTCCCT GCGCCCTCTC GCACGATTAC CATAAAAGGC AATGGCTGCG GCTCGCCGCG CCTCGACAGC CGCCGGCGCT CCGGGGGCCG CCGCGCCCCT CCCCCGAGCC CTCCCCGGCC CGAGGCGGCC CCGCCCCGCC CGGCACCCCC ACCTGCCGCC ACCCCCCGCC CGGCACGGCG AGCCCCGCGC CACGCCCCGT ACGGAGCCCC GCACCCGAAG CCGGGCCGTG CTCAGCAACT CGGGGAGGGG GGTGCAGGGG GGGTTGCAGC CCGACCGACG CGCCCACACC CCCTGCTCAC CCCCCCACGC ACACACCCCG CACGCAGCCT TTGTTCCCCT CGCAGCCCCC CCCGCACCGC GGGGCACCGC CCCCGGCCGC GCTCCCCTCG CGCACACTGC GGAGCGCACA AAGCCCCGCG CCGCGCCCGC AGCGCTCACA GCCGCCGGGC AGCGCGGAGC CGCACGCGGC GCTCCCCACG CACACACACA CGCACGCACC CCCCGAGCCG CTCCCCCCGC ACAAAGGGCC CTCCCGGAGC CCCTCAAGGC TTTCACGCAG CCACAGAAAA GAAACAAGCC GTCATTAAAC CAAGCGCTAA TTACAGCCCG GAGGAGAAGG GCCGTCCCGC CCGCTCACCT GTGGGAGTAA CGCGGTCAGT CAGAGCCGGG GCGGGCGGCG CGAGGCGGCG GCGGAGCGGG GCACGGGGCG AAGGCAGCGT CGCAGCGACT CCCCGCCCGC CGCGCGCTTC GCTTTTTATA GGGCCGCCGC CGCCGCCGCC TCGCCATAAA AGGAAACTTT CGGAGCGCGC CGCTCTGATT GGCTGCCGCC GCACCTCTCC GCCTCGCCCC GCCCCGCCCC TCGCCCCGCC CCGCCCCGCC TGGCGCGCGC CCCCCCCCCC CCCCCGCCCC CATCGCTGCA CAAAATAATT AAAAAATAAA TAAATACAAA ATTGGGGGTG GGGAGGGGGG GGAGATGGGG AGAGTGAAGC AGAACGTGGG GCTCACCTCG ACCATGGTAA TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA GTCCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG TAAATACTCC ACCCATTGAC GTCAATGGAA AGTCCCTATT GGCGTTACTA TGGGAACATA CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC CGTAAGTTAT GTAACGCGGA ACTCCATATA TGGGCTATGA ACTAATGACC CCGTAATTGA TTACTATTAA TAACTAGTCA ATAATCAATG TCGAGACCCA TAGAGCCCAC CGCATCCCCA GCATGCCTGC TATTGTCTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC CCCACCCCCC AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA TTAGGAAAGG ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGGG TTTAAACTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGGTCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCATCG CCGTCGGGCA TGCGCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCC TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATG GCCGATCCCA TGGTGGCGGT ACCCTCGAGC CTAGGGCCGG GATTCTCCTC CACGTCACCG CATGTTAGAA GACTTCCTCT GCCCTCTCCG CTGCCAGATC TCTCGAGGCC CTGTGGGAGG AAGAGAAGAG GTCAGAAGCT TATAACTTCG TATAATGTAT GCTATACGAA GTTATTGCCC CACTGTGGGG TGGAGGGGAC AGATAAAAGT ACCCAGAACC AGAGCCACAT TAACCGGCCC TGGGAATATA AGGTGGTCCC AGCTCGGGGA CACAGGATCC CTGGAGGCAG CAAACATGCT GTCCTGAAGT GGACATAGGG GCCCGGGTTG GAGGAAGAAG ACTAGCTGAG CTCTCGGACC CCTGGAAGAT GCCATGACAG GGGGCTGGAA GAGCTAGCAC AGACTAGAGA GGTAAGGGGG GTAGGGGAGC TGCCCAAATG AAAGGAGTGA GAGGTGACCC GAATCCACAG GAGAACGGGG TGTCCAGGCA AAGAAAGCAA GAGGATGGAG AGGTGGCTAA AGCCAGGGAG ACGGGGTACT TTGGGGTTGT CCAGAAAAAC GGTGATGATG CAGGCCTACA AGAAGGGGAG GCGGGACGCA AGGGAGACAT CCGTCGGAGA AGGCCATCCT AAGAAACGAG AGATGGCACA GGCCCCAGAA GGAGAAGGAA AAGGGAACCC AGCGAGTGAA GACGGCATGG GGTTGGGTGA GGGAGGAGAG ATGCCCGGAG AGGACCCAGA CACGGGGAGG ATCCGCTCAG AGGACATCAC GTGGTGCAGC GCCGAGAAGG AAGTGCTCCG GAAAGAGCAT CCTTGGGCAG CAACACAGCA GAGAGCAAGG GGAAGAGGGA GTGGAGGAAG ACGGAACCTG AAGGAGGCGG CAGGGAAGGA TCTGGGCCAG CCGTAGAGGT GACCCAGGCC ACAAGCTGCA GACAGAAAGC GGCACAGGCC CAGGGGAGAG AATGCTGGTC AGAGAAAGCA
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