pYX-Asc-Smarca4(mouse)
$113.00
In stock
SKU
PL63943
NM_011417.3;b2b508.1C; b2b508.1Clo; b2b692C; b2b692Clo
100μg liquid plasmid(Instantly):$134
| name | pYX-Asc-Smarca4(mouse) | another name | apYX-Asc-Smarca4(mouse) |
| Insert Size (bp) | 4845bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | T3：ATTAACCCTCACTAAAGGGA | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGGGGCCCC CGGCCGGGCG CGTGCCGTGG GGGGGGGTGG CCTGGCGGAA GCCCAGCGGG CGCGCGCGCG AGGCTTTCCC ACTCGCTTGG CAGCGGCGGA GACGGCTTCT TTGTTTCCTG AGGAGAAGCG AGACGCCCAC TCTGTCCCCG ACCCCTCGTG GAGGGTTGGG GGCGGCGCCA GGAAGGTTAC GGCGCCGTTA CCTCCAGGAG ACCAGTGCCT GTAGCTCCAG TAAAGATGTC TACTCCAGAC CCACCCTTGG GTGGGACTCC TCGGCCTGGT CCTTCCCCAG GCCCTGGTCC TTCACCTGGT GCAATGCTGG GTCCTAGCCC TGGCCCCTCA CCAGGTTCTG CCCACAGCAT GATGGGGCCA AGCCCAGGAC CTCCTTCAGC AGGACATCCC ATGCCCACCC AGGGGCCTGG AGGGTACCCC CAGGACAACA TGCATCAGAT GCACAAGCCT ATGGAGTCCA TGCACGAGAA GGGCATGCCT GATGACCCAC GATACAACCA GATGAAAGGG ATGGGCATGC GGTCAGGGGC CCACACAGGC ATGGCACCTC CACCTAGTCC CATGGACCAG CATTCTCAAG GTTACCCCTC ACCCCTCGGC GGCTCTGAAC ATGCCTCCAG TCCTGTCCCA GCCAGTGGCC CATCTTCAGG CCCCCAGATG TCCTCTGGGC CAGGAGGGGC CCCACTAGAT GGTTCTGATC CCCAGGCCTT GGGACAGCAA AACAGAGGCC CAACCCCATT TAACCAGAAC CAGCTGCATC AACTCAGAGC TCAGATAATG GCCTACAAGA TGTTGGCCAG GGGCCAGCCA TTGCCCGACC ACCTGCAGAT GGCCGTGCAA GGCAAGCGGC CGATGCCTGG AATGCAGCAA CAGATGCCAA CACTACCTCC ACCCTCAGTG TCCGCCACAG GACCCGGACC TGGACCCGGC CCTGGCCCTG GCCCTGGCCC AGGACCAGCC CCTCCAAATT ACAGTAGACC CCATGGTATG GGAGGGCCCA ACATGCCTCC CCCAGGACCC TCAGGTGTGC CCCCCGGGAT GCCTGGTCAG CCGCCTGGAG GGCCTCCCAA GCCATGGCCT GAAGGACCCA TGGCCAATGC TGCTGCCCCC ACAAGCACCC CACAGAAGCT GATTCCTCCG CAACCAACAG GCCGTCCTTC ACCTGCACCT CCTGCTGTCC CGCCTGCTGC CTCACCTGTA ATGCCACCAC AAACACAGTC CCCAGGGCAG CCAGCCCAGC CTGCTCCATT GGTGCCACTG CACCAGAAGC AGAGCCGAAT CACCCCCATC CAGAAGCCCC GAGGCCTTGA CCCTGTGGAG ATCCTACAAG AGCGGGAGTA CAGGCTTCAG GCTCGAATCG CACACAGAAT TCAGGAACTT GAAAACCTCC CTGGGTCCCT GGCTGGGGAC CTTCGAACCA AAGCAACCAT CGAACTCAAG GCCCTTAGGT TGCTGAACTT CCAGAGGCAG CTGCGCCAGG AGGTGGTGGT GTGCATGCGA AGAGACACAG CCCTGGAGAC AGCCCTCAAT GCCAAGGCCT ACAAGCGCAG CAAACGTCAG TCACTACGGG AGGCCCGCAT CACTGAGAAG TTGGAGAAGC AGCAGAAGAT TGAACAGGAG CGCAAGCGCC GCCAGAAGCA CCAGGAGTAC CTCAACAGCA TTCTGCAGCA TGCCAAGGAC TTCAGGGAGT ATCACAGATC AGTCACAGGC AAACTCCAGA AACTCACCAA GGCTGTGGCC ACCTACCATG CCAACACTGA GCGGGAGCAG AAGAAAGAAA ATGAGCGCAT TGAGAAGGAG CGAATGCGGA GGCTTATGGC TGAAGATGAG GAGGGCTACC GCAAACTCAT TGACCAGAAG AAGGACAAGC GCCTGGCCTA CCTTCTGCAG CAGACAGATG AGTATGTGGC CAACCTCACA GAGCTGGTGC GGCAGCACAA AGCTGCCCAG GTTGCCAAGG AGAAGAAGAA GAAAAAGAAA AAGAAGAAGG CAGAAAATGC TGAAGGACAG ACACCTGCTA TTGGACCAGA TGGTGAGCCT CTGGATGAGA CCAGCCAGAT GAGTGACCTC CCTGTGAAGG TGATCCACGT GGAGAGTGGC AAGATCCTCA CTGGCACAGA TGCCCCAAAA GCCGGGCAGC TGGAAGCCTG GCTTGAAATG AACCCAGGGT ATGAAGTAGC CCCCAGGTCA GACAGTGAAG AAAGTGGCTC TGAAGAGGAG GAGGAGGAGG AGGAAGAGGA GCAGCCTCAG CCCGCACAGC CCCCTACACT GCCTGTGGAA GAAAAGAAGA AGATTCCAGA CCCAGACAGC GATGATGTCT CTGAGGTGGA CGCCCGACAC ATTATTGAGA ACGCCAAGCA AGATGTGGAC GATGAGTACG GTGTGTCCCA GGCCCTTGCT CGTGGCCTGC AGTCTTACTA TGCTGTGGCC CATGCAGTCA CAGAGAGAGT AGATAAGCAG TCCGCCCTCA TGGTCAACGG TGTCCTCAAA CAGTACCAGA TCAAGGGTTT GGAGTGGCTG GTGTCCCTGT ACAACAACAA CCTGAATGGC ATCCTGGCTG ATGAGATGGG GCTGGGGAAG ACCATCCAGA CCATCGCGCT CATCACATAC CTCATGGAGC ACAAGCGCAT CAACGGGCCT TTCCTCATCA TCGTGCCTCT CTCGACACTG TCAAACTGGG CGTATGAATT TGACAAGTGG GCCCCCTCTG TGGTGAAGGT TTCTTACAAG GGCTCTCCAG CTGCAAGGCG AGCTTTTGTC CCACAGCTTC GCAGTGGGAA GTTCAACGTC TTACTGACCA CCTATGAATA TATCATCAAA GACAAGCATA TCCTAGCCAA GATCCGCTGG AAGTACATGA TTGTGGATGA AGGCCACCGC ATGAAAAACC ACCACTGCAA GTTGACGCAG GTCCTTAACA CACACTACGT GGCCCCTCGG CGCCTGCTTC TTACAGGCAC ACCACTGCAG AACAAGCTAC CGGAGCTCTG GGCCCTGCTT AACTTCCTGC TCCCCACTAT CTTCAAGAGC TGCAGCACCT TCGAACAGTG GTTCAATGCA CCCTTTGCCA TGACTGGAGA AAAGGTGGAC CTGAATGAAG AGGAGACTAT CCTCATTATT CGTCGCCTAC ACAAAGTTCT GCGGCCCTTC CTGCTGCGGC GGCTCAAGAA GGAAGTTGAA GCCCAGCTCC CTGAGAAGGT AGAGTATGTC ATCAAATGCG ACATGTCAGC CCTGCAGCGT GTGCTGTACC GTCACATGCA GGCCAAAGGT GTGCTGCTGA CTGACGGCTC CGAGAAGGAC AAGAAGGGCA AAGGTGGCAC CAAGACACTG ATGAACACTA TTATGCAACT GCGTAAGATC TGCAACCACC CCTACATGTT CCAGCACATC GAGGAGTCCT TTTCTGAGCA CTTGGGGTTC ACCGGCGGCA TCGTGCAAGG ATTGGACCTT TACCGTGCCT CAGGGAAATT TGAACTTCTT GATAGAATTC TACCCAAACT CCGTGCAACG AACCATAAAG TGCTCCTCTT TTGCCAAATG ACCTCCCTCA TGACCATCAT GGAAGACTAC TTTGCATACC GTGGCTTCAA ATACCTCAGG CTTGATGGAA CCACAAAAGC AGAAGACCGG GGCATGCTGT TGAAAACCTT TAATGAACCT GGCTCTGAGT ATTTCATTTT CCTGCTCAGT ACCCGTGCTG GGGGGCTGGG CCTGAATCTG CAGTCAGCTG ACACTGTGAT CATCTTTGAC AGTGACTGGA ATCCCCACCA GGACCTGCAA GCACAGGATC GAGCCCATCG CATTGGACAG CAGAATGAGG TGCGTGTTCT TCGCCTGTGC ACGGTCAACA GTGTGGAAGA GAAGATACTG GCTGCTGCCA AATACAAACT CAATGTGGAT CAGAAGGTGA TCCAGGCAGG CATGTTCGAC CAGAAGTCGT CCAGCCATGA GAGGCGTGCC TTCCTGCAGG CCATCCTGGA GCACGAGGAG CAGGATGAGG AGGAAGATGA GGTGCCTGAT GATGAGACCG TCAACCAGAT GATTGCCCGG CACGAAGAAG AGTTTGACCT CTTCATGCGC ATGGACTTGG ACCGCCGGCG TGAAGAAGCC CGCAACCCCA AGCGGAAGCC ACGCCTGATG GAAGAGGATG AGCTCCCATC CTGGATCATC AAGGATGATG CCGAGGTGGA GCGGCTGACA TGTGAAGAGG AAGAGGAGAA GATGTTCGGC CGTGGTTCTC GCCACCGCAA GGAGGTAGAC TACAGCGACT CACTGACAGA GAAGCAGTGG CTCAAGGCTA TCGAGGAGGG CACGCTGGAG GAGATCGAAG AGGAGGTCCG GCAGAAGAAA TCTTCACGTA AGCGTAAGCG AGACAGCGAG GCCGGCTCCT CCACCCCGAC CACCAGCACC CGCAGCCGTG ACAAGGATGA GGAGAGCAAG AAGCAGAAGA AACGTGGGCG GCCACCTGCT GAGAAGCTGT CCCCAAACCC ACCTAACCTC ACCAAGAAGA TGAAGAAGAT CGTGGATGCT GTGATCAAGT ACAAAGACAG CAGCAGTGGA CGTCAGCTCA GCGAGGTGTT CATCCAGCTC CCCTCTCGCA AGGAGCTTCC TGAGTACTAT GAGCTCATCC GAAAGCCTGT GGACTTCAAG AAGATCAAGG AACGCATCCG AAACCACAAG TACCGCAGCC TCAATGACCT GGAGAAGGAT GTGATGCTGC TGTGCCAGAA CGCTCAGACG TTCAACCTCG AGGGTTCCCT GATCTATGAG GACTCCATCG TCCTGCAGTC TGTCTTCACC AGCGTACGGC AGAAGATTGA GAAGGAGGAC GACAGTGAAG GCGAGGAAAG CGAGGAGGAG GAGGAGGGCG AGGAGGAAGG CTCCGAGTCT GAGTCCCGCT CCGTCAAGGT GAAGATCAAG CTGGGCCGCA AGGAGAAGGC CCAGGACCGA CTCAAGGGGG GCCGCCGGCG GCCAAGCCGG GGATCCCGGG CCAAGCCGGT TGTGAGTGAC GATGACAGTG AGGAGGAGCA GGAGGAGGAC CGCTCAGGAA GTGGCAGTGA GGAAGACTGA ACCAGACATT CCTGAGTCCT GACCCCGAGG CGCTCGTCCC AGCCAAGATG GAGTAGCCCT TAGCAGTGAT GGGTAGCACC AGATGTAGTT TCGAACTTGG AGAACTGTAC ACATGCAATC TTCCACATTT TTAGGCAGAG AAGTATAGGC CTGTCTGTCG GCCCTGGCCT GGCCTCGAGT CTCTACCAGC ATTAACTGTC TAGAGAGGGG ACCTCCTGGG AGCACCATCC ACCTCCCCAG GCCCCAGTCA CTGTAGCTCA GTGGATGCAT GCGCGTGCCG GCCGCTCCTT GTACTGTATC TTACTGGACA GGGCCAGCTC TCCAGGAGGC TCACAGGCCC AGCGGGTATG TCAGTGTCAC TGGAGTCAGA CAGTAATAAA TTAAAGCAAT GACAAGCCAA AAAAAAAAAA AAAAAACTGT CTCGCTGCGG CCGCTCGCTA CCTTAGGACC GTTATAGTTA GGTCCCTTTA GTGAGGGTTA ATTAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAA
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