pMDLg-pRRE-Kan
$99.00
In stock
SKU
PL63935
100μg liquid plasmid(Instantly):$120
| name | pMDLg-pRRE-Kan | another name | apMDLg-pRRE-Kan |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Virus | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTCCAACATT ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC CCTCGAAGCT TACATGTGGT ACCGAGCTCG GATCCTGAGA ACTTCAGGGT GAGTCTATGG GACCCTTGAT GTTTTCTTTC CCCTTCTTTT CTATGGTTAA GTTCATGTCA TAGGAAGGGG AGAAGTAACA GGGTACACAT ATTGACCAAA TCAGGGTAAT TTTGCATTTG TAATTTTAAA AAATGCTTTC TTCTTTTAAT ATACTTTTTT GTTTATCTTA TTTCTAATAC TTTCCCTAAT CTCTTTCTTT CAGGGCAATA ATGATACAAT GTATCATGCC TCTTTGCACC ATTCTAAAGA ATAACAGTGA TAATTTCTGG GTTAAGGCAA TAGCAATATT TCTGCATATA AATATTTCTG CATATAAATT GTAACTGATG TAAGAGGTTT CATATTGCTA ATAGCAGCTA CAATCCAGCT ACCATTCTGC TTTTATTTTA TGGTTGGGAT AAGGCTGGAT TATTCTGAGT CCAAGCTAGG CCCTTTTGCT AATCATGTTC ATACCTCTTA TCTTCCTCCC ACAGCTCCTG GGCAACGTGC TGGTCTGTGT GCTGGCCCAT CACTTTGGCA AAGCACGTGA GATCTGAATT CGAGATCTGC CGCCGCCATG GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GATGGGAAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGAAAAAAG CACAGCAAGC AGCAGCTGAC ACAGGACACA GCAATCAGGT CAGCCAAAAT TACCCTATAG TGCAGAACAT CCAGGGGCAA ATGGTACATC AGGCCATATC ACCTAGAACT TTAAATGCAT GGGTAAAAGT AGTAGAAGAG AAGGCTTTCA GCCCAGAAGT GATACCCATG TTTTCAGCAT TATCAGAAGG AGCCACCCCA CAAGATTTAA ACACCATGCT AAACACAGTG GGGGGACATC AAGCAGCCAT GCAAATGTTA AAAGAGACCA TCAATGAGGA AGCTGCAGAA TGGGATAGAG TGCATCCAGT GCATGCAGGG CCTATTGCAC CAGGCCAGAT GAGAGAACCA AGGGGAAGTG ACATAGCAGG AACTACTAGT ACCCTTCAGG AACAAATAGG ATGGATGACA CATAATCCAC CTATCCCAGT AGGAGAAATC TATAAAAGAT GGATAATCCT GGGATTAAAT AAAATAGTAA GAATGTATAG CCCTACCAGC ATTCTGGACA TAAGACAAGG ACCAAAGGAA CCCTTTAGAG ACTATGTAGA CCGATTCTAT AAAACTCTAA GAGCCGAGCA AGCTTCACAA GAGGTAAAAA ATTGGATGAC AGAAACCTTG TTGGTCCAAA ATGCGAACCC AGATTGTAAG ACTATTTTAA AAGCATTGGG ACCAGGAGCG ACACTAGAAG AAATGATGAC AGCATGTCAG GGAGTGGGGG GACCCGGCCA TAAAGCAAGA GTTTTGGCTG AAGCAATGAG CCAAGTAACA AATCCAGCTA CCATAATGAT ACAGAAAGGC AATTTTAGGA ACCAAAGAAA GACTGTTAAG TGTTTCAATT GTGGCAAAGA AGGGCACATA GCCAAAAATT GCAGGGCCCC TAGGAAAAAG GGCTGTTGGA AATGTGGAAA GGAAGGACAC CAAATGAAAG ATTGTACTGA GAGACAGGCT AATTTTTTAG GGAAGATCTG GCCTTCCCAC AAGGGAAGGC CAGGGAATTT TCTTCAGAGC AGACCAGAGC CAACAGCCCC ACCAGAAGAG AGCTTCAGGT TTGGGGAAGA GACAACAACT CCCTCTCAGA AGCAGGAGCC GATAGACAAG GAACTGTATC CTTTAGCTTC CCTCAGATCA CTCTTTGGCA GCGACCCCTC GTCACAATAA AGATAGGGGG GCAATTAAAG GAAGCTCTAT TAGATACAGG AGCAGATGAT ACAGTATTAG AAGAAATGAA TTTGCCAGGA AGATGGAAAC CAAAAATGAT AGGGGGAATT GGAGGTTTTA TCAAAGTAAG ACAGTATGAT CAGATACTCA TAGAAATCTG CGGACATAAA GCTATAGGTA CAGTATTAGT AGGACCTACA CCTGTCAACA TAATTGGAAG AAATCTGTTG ACTCAGATTG GCTGCACTTT AAATTTTCCC ATTAGTCCTA TTGAGACTGT ACCAGTAAAA TTAAAGCCAG GAATGGATGG CCCAAAAGTT AAACAATGGC CATTGACAGA AGAAAAAATA AAAGCATTAG TAGAAATTTG TACAGAAATG GAAAAGGAAG GAAAAATTTC AAAAATTGGG CCTGAAAATC CATACAATAC TCCAGTATTT GCCATAAAGA AAAAAGACAG TACTAAATGG AGAAAATTAG TAGATTTCAG AGAACTTAAT AAGAGAACTC AAGATTTCTG GGAAGTTCAA TTAGGAATAC CACATCCTGC AGGGTTAAAA CAGAAAAAAT CAGTAACAGT ACTGGATGTG GGCGATGCAT ATTTTTCAGT TCCCTTAGAT AAAGACTTCA GGAAGTATAC TGCATTTACC ATACCTAGTA TAAACAATGA GACACCAGGG ATTAGATATC AGTACAATGT GCTTCCACAG GGATGGAAAG GATCACCAGC AATATTCCAG TGTAGCATGA CAAAAATCTT AGAGCCTTTT AGAAAACAAA ATCCAGACAT AGTCATCTAT CAATACATGG ATGATTTGTA TGTAGGATCT GACTTAGAAA TAGGGCAGCA TAGAACAAAA ATAGAGGAAC TGAGACAACA TCTGTTGAGG TGGGGATTTA CCACACCAGA CAAAAAACAT CAGAAAGAAC CTCCATTCCT TTGGATGGGT TATGAACTCC ATCCTGATAA ATGGACAGTA CAGCCTATAG TGCTGCCAGA AAAGGACAGC TGGACTGTCA ATGACATACA GAAATTAGTG GGAAAATTGA ATTGGGCAAG TCAGATTTAT GCAGGGATTA AAGTAAGGCA ATTATGTAAA CTTCTTAGGG GAACCAAAGC ACTAACAGAA GTAGTACCAC TAACAGAAGA AGCAGAGCTA GAACTGGCAG AAAACAGGGA GATTCTAAAA GAACCGGTAC ATGGAGTGTA TTATGACCCA TCAAAAGACT TAATAGCAGA AATACAGAAG CAGGGGCAAG GCCAATGGAC ATATCAAATT TATCAAGAGC CATTTAAAAA TCTGAAAACA GGAAAGTATG CAAGAATGAA GGGTGCCCAC ACTAATGATG TGAAACAATT AACAGAGGCA GTACAAAAAA TAGCCACAGA AAGCATAGTA ATATGGGGAA AGACTCCTAA ATTTAAATTA CCCATACAAA AGGAAACATG GGAAGCATGG TGGACAGAGT ATTGGCAAGC CACCTGGATT CCTGAGTGGG AGTTTGTCAA TACCCCTCCC TTAGTGAAGT TATGGTACCA GTTAGAGAAA GAACCCATAA TAGGAGCAGA AACTTTCTAT GTAGATGGGG CAGCCAATAG GGAAACTAAA TTAGGAAAAG CAGGATATGT AACTGACAGA GGAAGACAAA AAGTTGTCCC CCTAACGGAC ACAACAAATC AGAAGACTGA GTTACAAGCA ATTCATCTAG CTTTGCAGGA TTCGGGATTA GAAGTAAACA TAGTGACAGA CTCACAATAT GCATTGGGAA TCATTCAAGC ACAACCAGAT AAGAGTGAAT CAGAGTTAGT CAGTCAAATA ATAGAGCAGT TAATAAAAAA GGAAAAAGTC TACCTGGCAT GGGTACCAGC ACACAAAGGA ATTGGAGGAA ATGAACAAGT AGATAAATTG GTCAGTGCTG GAATCAGGAA AGTACTATTT TTAGATGGAA TAGATAAGGC CCAAGAAGAA CATGAGAAAT ATCACAGTAA TTGGAGAGCA ATGGCTAGTG ATTTTAACCT ACCACCTGTA GTAGCAAAAG AAATAGTAGC CAGCTGTGAT AAATGTCAGC TAAAAGGGGA AGCCATGCAT GGACAAGTAG ACTGTAGCCC AGGAATATGG CAGCTAGATT GTACACATTT AGAAGGAAAA GTTATCTTGG TAGCAGTTCA TGTAGCCAGT GGATATATAG AAGCAGAAGT AATTCCAGCA GAGACAGGGC AAGAAACAGC ATACTTCCTC TTAAAATTAG CAGGAAGATG GCCAGTAAAA ACAGTACATA CAGACAATGG CAGCAATTTC ACCAGTACTA CAGTTAAGGC CGCCTGTTGG TGGGCGGGGA TCAAGCAGGA ATTTGGCATT CCCTACAATC CCCAAAGTCA AGGAGTAATA GAATCTATGA ATAAAGAATT AAAGAAAATT ATAGGACAGG TAAGAGATCA GGCTGAACAT CTTAAGACAG CAGTACAAAT GGCAGTATTC ATCCACAATT TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA AATTTTCGGG TTTATTACAG GGACAGCAGA GATCCAGTTT GGAAAGGACC AGCAAAGCTC CTCTGGAAAG GTGAAGGGGC AGTAGTAATA CAAGATAATA GTGACATAAA AGTAGTGCCA AGAAGAAAAG CAAAGATCAT CAGGGATTAT GGAAAACAGA TGGCAGGTGA TGATTGTGTG GCAAGTAGAC AGGATGAGGA TTAACACATG GAATTCCGGA GCGGCCGCAG GAGCTTTGTT CCTTGGGTTC TTGGGAGCAG CAGGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT ACAGGCCAGA CAATTATTGT CTGGTATAGT GCAGCAGCAG AACAATTTGC TGAGGGCTAT TGAGGCGCAA CAGCATCTGT TGCAACTCAC AGTCTGGGGC ATCAAGCAGC TCCAGGCAAG AATCCTGGCT GTGGAAAGAT ACCTAAAGGA TCAACAGCTC CTGGGGATTT GGGGTTGCTC TGGAAAACTC ATTTGCACCA CTGCTGTGCC TTGGAATGCT AGTTGGAGTA ATAAATCTCT GGAACAGATT TGGAATCACA CGACCTGGAT GGAGTGGGAC AGAGAAATTA ACAATTACAC AAGCTTCCGC GGAATTCACC CCACCAGTGC AGGCTGCCTA TCAGAAAGTG GTGGCTGGTG TGGCTAATGC CCTGGCCCAC AAGTATCACT AAGCTCGCTT TCTTGCTGTC CAATTTCTAT TAAAGGTTCC TTTGTTCCCT AAGTCCAACT ACTAAACTGG GGGATATTAT GAAGGGCCTT GAGCATCTGG ATTCTGCCTA ATAAAAAACA TTTATTTTCA TTGCAATGAT GTATTTAAAT TATTTCTGAA TATTTTACTA AAAAGGGAAT GTGGGAGGTC AGTGCATTTA AAACATAAAG AAATGAAGAG CTAGTTCAAA CCTTGGGAAA ATACACTATA TCTTAAACTC CATGAAAGAA GGTGAGGCTG CAAACAGCTA ATGCACATTG GCAACAGCCC CTGATGCCTA TGCCTTATTC ATCCCTCAGA AAAGGATTCA AGTAGAGGCT TGATTTGGAG GTTAAAGTTT TGCTATGCTG TATTTTACAT TACTTATTGT TTTAGCTGTC CTCATGAATG TCTTTTCACT ACCCATTTGC TTATCCTGCA TCTCTCAGCC TTGACTCCAC TCAGTTCTCT TGCTTAGAGA TACCACCTTT CCCCTGAAGT GTTCCTTCCA TGTTTTACGG CGAGATGGTT TCTCCTCGCC TGGCCACTCA GCCTTAGTTG TCTCTGTTGT CTTATAGAGG TCTACTTGAA GAAGGAAAAA CAGGGGGCAT GGTTTGACTG TCCTGTGAGC CCTTCTTCCC TGCCTCCCCC ACTCACAGTG ACCCGGAATC CCTCGACATG GCAGTCTAGC ACTAGTGCGG CCGCAGATCT GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTGGATC CCCTGAGGGG GCCCCCATGG GCTAGAGGAT CCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGAGCTTG GCCCATTGCA TACGTTGTAT CCATATCATA ATATGTACAT TTATATTGGC TCAT
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