pBI-RNAi-WRKY15-1
$113.00
In stock
SKU
PL63804
100μg liquid plasmid(Instantly):$134
| name | pBI-RNAi-WRKY15-1 | another name | apBI-RNAi-WRKY15-1 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | Gus |
| Promoter | Replicon | oriV | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA CGTTGAAGGA GCCACTCAGC CGCGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGCCT CCTGTCAATG CTGGCGGCGG CTCTGGTGGT GGTTCTGGTG GCGGCTCTGA GGGTGGTGGC TCTGAGGGTG GCGGTTCTGA GGGTGGCGGC TCTGAGGGAG GCGGTTCCGG TGGTGGCTCT GGTTCCGGTG ATTTTGATTA TGAAAAGATG GCAAACGCTA ATAAGGGGGC TATGACCGAA AATGCCGATG AAAACGCGCT ACAGTCTGAC GCTAAAGGCA AACTTGATTC TGTCGCTACT GATTACGGTG CTGCTATCGA TGGTTTCATT GGTGACGTTT CCGGCCTTGC TAATGGTAAT GGTGCTACTG GTGATTTTGC TGGCTCTAAT TCCCAAATGG CTCAAGTCGG TGACGGTGAT AATTCACCTT TAATGAATAA TTTCCGTCAA TATTTACCTT CCCTCCCTCA ATCGGTTGAA TGTCGCCCTT TTGTCTTTGG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC CCCAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT ACGCAGCAGG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTCCAGGAA ATCAAATACC TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG GGACTCTAGA GCCAATTTGT GACGAGGTTT TGGTTGAACA AATGGCTACT TATTTAATCA AAGACTCTAA ATTTACGGAA GAACTTGCTG CTGCAATGTA TTCCAAGACT TTAGAAGCTA GTAATTTTTT ATTCAAAGTA TGTTAATGTA TTAGCACTGA TTCTTCCTTA TGGTTCATAA AGAAATAAAA TTTTAAGTGT ATTTAGTGGT GGATCCAAGC TTATCGATTT CGAACCCAGC TTCCCAACTG TAATCAATCC AAATGTAAGA TCAATGATAA CACAATGACA TGATCTATCA TGTTACCTTG TTTATTCATG TTCGACTAAT TCATTTAATT AATAGTCAAT CCATTTAGAA GTTAATAAAA CTACAAGTAT TATTTAGAAA TTAATAAGAA TGTTGATTGA AAAATAATAC TATATAAAAT TGATAGATCT TGCGCTTTGT TATATTAGCA TTAGATTATG TTTTGTTACA TTAGATTACT GTTTCTATTA GTTTGATATT ATTTGTTACT TTAGCTTGTT ATTTAATATT TTGTTTATTG ATAAATTACA AGCAGATTGG AATTTCTAAC AAAATATTTA TTAACTTTTA AACTAAAATA TTTAGTAATG GTATAGATAT TTAATTATAT AATAAACTAT TAATCATAAA AAAATATTAT TTTAATTTAT TTATTCTTAT TTTTACTATA GTATTTTATC ATTGATATTT AATTCATCAA ACCAGCTAGA ATTACTATTA TGATTAAAAC AAATATTAAT GCTAGTATAT CATCTTACAT GTTCGATCAA ATTCATTAAA AATAATATAC TTACTCTCAA CTTTTATCTT CTTCGTCTTA CACATCACTT GTCATATTTT TTTACATTAC TATGTTGTTT ATGTAAACAA TATATTTATA AATTATTTTT TCACAATTAT AACAACTATA TTATTATAAT CATACTAATT AACATCACTT AACTATTTTA TACTAAAAGG AAAAAAGAAA ATAATTATTT CCTTACCAAT TGGGGTACCA CCACTAAATA CACTTAAAAT TTTATTTCTT TATGAACCAT AAGGAAGAAT CAGTGCTAAT ACATTAACAT ACTTTGAATA AAAAATTACT AGCTTCTAAA GTCTTGGAAT ACATTGCAGC AGCAAGTTCT TCCGTAAATT TAGAGTCTTT GATTAAATAA GTAGCCATTT GTTCAACCAA AACCTCGTCA CAAATTGGCG AGCTCGGTCA GTCCCTTATG TTACGTCCTG TAGAAACCCC AACCCGTGAA ATCAAAAAAC TCGACGGCCT GTGGGCATTC AGTCTGGATC GCGAAAACTG TGGAATTGAT CAGCGTTGGT GGGAAAGCGC GTTACAAGAA AGCCGGGCAA TTGCTGTGCC AGGCAGTTTT AACGATCAGT TCGCCGATGC AGATATTCGT AATTATGCGG GCAACGTCTG GTATCAGCGC GAAGTCTTTA TACCGAAAGG TTGGGCAGGC CAGCGTATCG TGCTGCGTTT CGATGCGGTC ACTCATTACG GCAAAGTGTG GGTCAATAAT CAGGAAGTGA TGGAGCATCA GGGCGGCTAT ACGCCATTTG AAGCCGATGT CACGCCGTAT GTTATTGCCG GGAAAAGTGT ACGTATCACC GTTTGTGTGA ACAACGAACT GAACTGGCAG ACTATCCCGC CGGGAATGGT GATTACCGAC GAAAACGGCA AGAAAAAGCA GTCTTACTTC CATGATTTCT TTAACTATGC CGGAATCCAT CGCAGCGTAA TGCTCTACAC CACGCCGAAC ACCTGGGTGG ACGATATCAC CGTGGTGACG CATGTCGCGC AAGACTGTAA CCACGCGTCT GTTGACTGGC AGGTGGTGGC CAATGGTGAT GTCAGCGTTG AACTGCGTGA TGCGGATCAA CAGGTGGTTG CAACTGGACA AGGCACTAGC GGGACTTTGC AAGTGGTGAA TCCGCACCTC TGGCAACCGG GTGAAGGTTA TCTCTATGAA CTGTGCGTCA CAGCCAAAAG CCAGACAGAG TGTGATATCT ACCCGCTTCG CGTCGGCATC CGGTCAGTGG CAGTGAAGGG CGAACAGTTC CTGATTAACC ACAAACCGTT CTACTTTACT GGCTTTGGTC GTCATGAAGA TGCGGACTTG CGTGGCAAAG GATTCGATAA CGTGCTGATG GTGCACGACC ACGCATTAAT GGACTGGATT GGGGCCAACT CCTACCGTAC CTCGCATTAC CCTTACGCTG AAGAGATGCT CGACTGGGCA GATGAACATG GCATCGTGGT GATTGATGAA ACTGCTGCTG TCGGCTTTAA CCTCTCTTTA GGCATTGGTT TCGAAGCGGG CAACAAGCCG AAAGAACTGT ACAGCGAAGA GGCAGTCAAC GGGGAAACTC AGCAAGCGCA CTTACAGGCG ATTAAAGAGC TGATAGCGCG TGACAAAAAC CACCCAAGCG TGGTGATGTG GAGTATTGCC AACGAACCGG ATACCCGTCC GCAAGGTGCA CGGGAATATT TCGCGCCACT GGCGGAAGCA ACGCGTAAAC TCGACCCGAC GCGTCCGATC ACCTGCGTCA ATGTAATGTT CTGCGACGCT CACACCGATA CCATCAGCGA TCTCTTTGAT GTGCTGTGCC TGAACCGTTA TTACGGATGG TATGTCCAAA GCGGCGATTT GGAAACGGCA GAGAAGGTAC TGGAAAAAGA ACTTCTGGCC TGGCAGGAGA AACTGCATCA GCCGATTATC ATCACCGAAT ACGGCGTGGA TACGTTAGCC GGGCTGCACT CAATGTACAC CGACATGTGG AGTGAAGAGT ATCAGTGTGC ATGGCTGGAT ATGTATCACC GCGTCTTTGA TCGCGTCAGC GCCGTCGTCG GTGAACAGGT ATGGAATTTC GCCGATTTTG CGACCTCGCA AGGCATATTG CGCGTTGGCG GTAACAAGAA AGGGATCTTC ACTCGCGACC GCAAACCGAA GTCGGCGGCT TTTCTGCTGC AAAAACGCTG GACTGGCATG AACTTCGGTG AAAAACCGCA GCAGGGAGGC AAACAATGAA TCAACAACTC TCCTGGCGCA CCATCGTCGG CTACAGCCTC GGGAATTGCT ACCCGAATTT CCCCGATCGT TCAAACATTT GGCAATAAAG TTTCTTAAGA TTGAATCCTG TTGCCGGTCT TGCGATGATT ATCATATAAT TTCTGTTGAA TTACGTTAAG CATGTAATAA TTAACATGTA ATGCATGACG TTATTTATGA GATGGGTTTT TATGATTAGA GTCCCGCAAT TATACATTTA ATACGCGATA GAAAACAAAA TATAGCGCGC AAACTAGGAT AAATTATCGC GCGCGGTGTC ATCTATGTTA CTAGATCGGG AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA CCACCCCAGT ACATTAAAAA CGTCCGCAAT GTGTTATTAA GTTGTCTAAG CGTCAATTTG TTTACACCAC AATATATCCT GCCACCAGCC AGCCAACAGC TCCCCGACCG GCAGCTCGGC ACAAAATCAC CACTCGATAC AGGCAGCCCA TCAGTCCGGG ACGGCGTCAG CGGGAGAGCC GTTGTAAGGC GGCAGACTTT GCTCATGTTA CCGATGCTAT TCGGAAGAAC GGCAACTAAG CTGCCGGGTT TGAAACACGG ATGATCTCGC GGAGGGTAGC ATGTTGATTG TAACGATGAC AGAGCGTTGC TGCCTGTGAT CAAATATCAT CTCCCTCGCA GAGATCCGAA TTATCAGCCT TCTTATTCAT TTCTCGCTTA ACCGTGACAG GCTGTCGATC TTGAGAACTA TGCCGACATA ATAGGAAATC GCTGGATAAA GCCGCTGAGG AAGCTGAGTG GCGCTATTTC TTTAGAAGTG AACGTTGACG ATATCAACTC CCCTATCCAT TGCTCACCGA ATGGTACAGG TCGGGGACCC GAAGTTCCGA CTGTCGGCCT GATGCATCCC CGGCTGATCG ACCCCAGATC TGGGGCTGAG AAAGCCCAGT AAGGAAACAA CTGTAGGTTC GAGTCGCGAG ATCCCCCGGA ACCAAAGGAA GTAGGTTAAA CCCGCTCCGA TCAGGCCGAG CCACGCCAGG CCGAGAACAT TGGTTCCTGT AGGCATCGGG ATTGGCGGAT CAAACACTAA AGCTACTGGA ACGAGCAGAA GTCCTCCGGC CGCCAGTTGC CAGGCGGTAA AGGTGAGCAG AGGCACGGGA GGTTGCCACT TGCGGGTCAG CACGGTTCCG AACGCCATGG AAACCGCCCC CGCCAGGCCC GCTGCGACGC CGACAGGATC TAGCGCTGCG TTTGGTGTCA ACACCAACAG CGCCACGCCC GCAGTTCCGC AAATAGCCCC CAGGACCGCC ATCAATCGTA TCGGGCTACC TAGCAGAGCG GCAGAGATGA ACACGACCAT CAGCGGCTGC ACAGCGCCTA CCGTCGCCGC GACCCCGCCC GGCAGGCGGT AGACCGAAAT AAACAACAAG CTCCAGAATA GCGAAATATT AAGTGCGCCG AGGATGAAGA TGCGCATCCA CCAGATTCCC GTTGGAATCT GTCGGACGAT CATCACGAGC AATAAACCCG CCGGCAACGC CCGCAGCAGC ATACCGGCGA CCCCTCGGCC TCGCTGTTCG GGCTCCACGA AAACGCCGGA CAGATGCGCC TTGTGAGCGT CCTTGGGGCC GTCCTCCTGT TTGAAGACCG ACAGCCCAAT GATCTCGCCG TCGATGTAGG CGCCGAATGC CACGGCATCT CGCAACCGTT CAGCGAACGC CTCCATGGGC TTTTTCTCCT CGTGCTCGTA AACGGACCCG AACATCTCTG GAGCTTTCTT CAGGGCCGAC AATCGGATCT CGCGGAAATC CTGCACGTCG GCCGCTCCAA GCCGTCGAAT CTGAGCCTTA ATCACAATTG TCAATTTTAA TCCTCTGTTT ATCGGCAGTT CGTAGAGCGC GCCGTGCGTC CCGAGCGATA CTGAGCGAAG CAAGTGCGTC GAGCAGTGCC CGCTTGTTCC TGAAATGCCA GTAAAGCGCT GGCTGCTGAA CCCCCAGCCG GAACTGACCC CACAAGGCCC TAGCGTTTGC AATGCACCAG GTCATCATTG ACCCAGGCGT GTTCCACCAG GCCGCTGCCT CGCAACTCTT CGCAGGCTTC GCCGACCTGC TCGCGCCACT TCTTCACGCG GGTGGAATCC GATCCGCACA TGAGGCGGAA GGTTTCCAGC TTGAGCGGGT ACGGCTCCCG GTGCGAGCTG AAATAGTCGA ACATCCGTCG GGCCGTCGGC GACAGCTTGC GGTACTTCTC CCATATGAAT TTCGTGTAGT GGTCGCCAGC AAACAGCACG ACGATTTCCT CGTCGATCAG GACCTGGCAA CGGGACGTTT TCTTGCCACG GTCCAGGACG CGGAAGCGGT GCAGCAGCGA CACCGATTCC AGGTGCCCAA CGCGGTCGGA CGTGAAGCCC ATCGCCGTCG CCTGTAGGCG CGACAGGCAT TCCTCGGCCT TCGTGTAATA CCGGCCATTG ATCGACCAGC CCAGGTCCTG GCAAAGCTCG TAGAACGTGA AGGTGATCGG CTCGCCGATA GGGGTGCGCT TCGCGTACTC CAACACCTGC TGCCACACCA GTTCGTCATC GTCGGCCCGC AGCTCGACGC CGGTGTAGGT GATCTTCACG TCCTTGTTGA CGTGGAAAAT GACCTTGTTT TGCAGCGCCT CGCGCGGGAT TTTCTTGTTG CGCGTGGTGA ACAGGGCAGA GCGGGCCGTG TCGTTTGGCA TCGCTCGCAT CGTGTCCGGC CACGGCGCAA TATCGAACAA GGAAAGCTGC ATTTCCTTGA TCTGCTGCTT CGTGTGTTTC AGCAACGCGG CCTGCTTGGC CTCGCTGACC TGTTTTGCCA GGTCCTCGCC GGCGGTTTTT CGCTTCTTGG TCGTCATAGT TCCTCGCGTG TCGATGGTCA TCGACTTCGC CAAACCTGCC GCCTCCTGTT CGAGACGACG CGAACGCTCC ACGGCGGCCG ATGGCGCGGG CAGGGCAGGG GGAGCCAGTT GCACGCTGTC GCGCTCGATC TTGGCCGTAG CTTGCTGGAC CATCGAGCCG ACGGACTGGA AGGTTTCGCG GGGCGCACGC ATGACGGTGC GGCTTGCGAT GGTTTCGGCA TCCTCGGCGG AAAACCCCGC GTCGATCAGT TCTTGCCTGT ATGCCTTCCG GTCAAACGTC CGATTCATTC ACCCTCCTTG CGGGATTGCC CCGACTCACG CCGGGGCAAT GTGCCCTTAT TCCTGATTTG ACCCGCCTGG TGCCTTGGTG TCCAGATAAT CCACCTTATC GGCAATGAAG TCGGTCCCGT AGACCGTCTG GCCGTCCTTC TCGTACTTGG TATTCCGAAT CTTGCCCTGC ACGAATACCA GCGACCCCTT GCCCAAATAC TTGCCGTGGG CCTCGGCCTG AGAGCCAAAA CACTTGATGC GGAAGAAGTC GGTGCGCTCC TGCTTGTCGC CGGCATCGTT GCGCCACATC TAGGTACTAA AACAATTCAT CCAGTAAAAT ATAATATTTT ATTTTCTCCC AATCAGGCTT GATCCCCAGT AAGTCAAAAA ATAGCTCGAC ATACTGTTCT TCCCCGATAT CCTCCCTGAT CGACCGGACG CAGAAGGCAA TGTCATACCA CTTGTCCGCC CTGCCGCTTC TCCCAAGATC AATAAAGCCA CTTACTTTGC CATCTTTCAC AAAGATGTTG CTGTCTCCCA GGTCGCCGTG GGAAAAGACA AGTTCCTCTT CGGGCTTTTC CGTCTTTAAA AAATCATACA GCTCGCGCGG ATCTTTAAAT GGAGTGTCTT CTTCCCAGTT TTCGCAATCC ACATCGGCCA GATCGTTATT CAGTAAGTAA TCCAATTCGG CTAAGCGGCT GTCTAAGCTA TTCGTATAGG GACAATCCGA TATGTCGATG GAGTGAAAGA GCCTGATGCA CTCCGCATAC AGCTCGATAA TCTTTTCAGG GCTTTGTTCA TCTTCATACT CTTCCGAGCA AAGGACGCCA TCGGCCTCAC TCATGAGCAG ATTGCTCCAG CCATCATGCC GTTCAAAGTG CAGGACCTTT GGAACAGGCA GCTTTCCTTC CAGCCATAGC ATCATGTCCT TTTCCCGTTC CACATCATAG GTGGTCCCTT TATACCGGCT GTCCGTCATT TTTAAATATA GGTTTTCATT TTCTCCCACC AGCTTATATA CCTTAGCAGG AGACATTCCT TCCGTATCTT TTACGCAGCG GTATTTTTCG ATCAGTTTTT TCAATTCCGG TGATATTCTC ATTTTAGCCA TTTATTATTT CCTTCCTCTT TTCTACAGTA TTTAAAGATA CCCCAAGAAG CTAATTATAA CAAGACGAAC TCCAATTCAC TGTTCCTTGC ATTCTAAAAC CTTAAATACC AGAAAACAGC TTTTTCAAAG TTGTTTTCAA AGTTGGCGTA TAACATAGTA TCGACGGAGC CGATTTTGAA ACCACAATTA TGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG GCTTCTGTGT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTATCTC TGCTCTCACT GCCGTAAAAC ATGGCAACTG CAGTTCACTT ACACCGCTTC TCAACCCGGT ACGCACCAGA AAATCATTGA TATGGCCATG AATGGCGTTG GATGCCGGGC AACAGCCCGC ATTATGGGCG TTGGCCTCAA CACGATTTTA CGTCACTTAA AAAACTCAGG CCGCAGTCGG TAACCTCGCG CATACAGCCG GGCAGTGACG TCATCGTCTG CGCGGAAATG GACGAACAGT GGGGCTATGT CGGGGCTAAA TCGCGCCAGC GCTGGCTGTT TTACGCGTAT GACAGTCTCC GGAAGACGGT TGTTGCGCAC GTATTCGGTG AACGCACTAT GGCGACGCTG GGGCGTCTTA TGAGCCTGCT GTCACCCTTT GACGTGGTGA TATGGATGAC GGATGGCTGG CCGCTGTATG AATCCCGCCT GAAGGGAAAG CTGCACGTAA TCAGCAAGCG ATATACGCAG CGAATTGAGC GGCATAACCT GAATCTGAGG CAGCACCTGG CACGGCTGGG ACGGAAGTCG CTGTCGTTCT CAAAATCGGT GGAGCTGCAT GACAAAGTCA TCGGGCATTA TCTGAACATA AAACACTATC AATAAGTTGG AGTCATTACC CAATTATGAT AGAATTTACA AGCTATAAGG TTATTGTCCT GGGTTTCAAG CATTAGTCCA TGCAAGTTTT TATGCTTTGC CCATTCTATA GATATATTGA TAAGCGCGCT GCCTATGCCT TGCCCCCTGA AATCCTTACA TACGGCGATA TCTTCTATAT AAAAGATATA TTATCTTATC AGTATTGTCA ATATATTCAA GGCAATCTGC CTCCTCATCC TCTTCATCCT CTTCGTCTTG GTAGCTTTTT AAATATGGCG CTTCATAGAG TAATTCTGTA AAGGTCCAAT TCTCGTTTTC ATACCTCGGT ATAATCTTAC CTATCACCTC AAATGGTTCG CTGGGTTTAT CGCACCCCCG AACACGAGCA CGGCACCCGC GACCACTATG CCAAGAATGC CCAAGGTAAA AATTGCCGGC CCCGCCATGA AGTCCGTGAA TGCCCCGACG GCCGAAGTGA AGGGCAGGCC GCCACCCAGG CCGCCGCCCT CACTGCCCGG CACCTGGTCG CTGAATGTCG ATGCCAGCAC CTGCGGCACG TCAATGCTTC CGGGCGTCGC GCTCGGGCTG ATCGCCCATC CCGTTACTGC CCCGATCCCG GCAATGGCAA GGACTGCCAG CGCTGCCATT TTTGGGGTGA GGCCGTTCGC GGCCGAGGGG CGCAGCCCCT GGGGGGATGG GAGGCCCGCG TTAGCGGGCC GGGAGGGTTC GAGAAGGGGG GGCACCCCCC TTCGGCGTGC GCGGTCACGC GCACAGGGCG CAGCCCTGGT TAAAAACAAG GTTTATAAAT ATTGGTTTAA AAGCAGGTTA AAAGACAGGT TAGCGGTGGC CGAAAAACGG GCGGAAACCC TTGCAAATGC TGGATTTTCT GCCTGTGGAC AGCCCCTCAA ATGTCAATAG GTGCGCCCCT CATCTGTCAG CACTCTGCCC CTCAAGTGTC AAGGATCGCG CCCCTCATCT GTCAGTAGTC GCGCCCCTCA AGTGTCAATA CCGCAGGGCA CTTATCCCCA GGCTTGTCCA CATCATCTGT GGGAAACTCG CGTAAAATCA GGCGTTTTCG CCGATTTGCG AGGCTGGCCA GCTCCACGTC GCCGGCCGAA ATCGAGCCTG CCCCTCATCT GTCAACGCCG CGCCGGGTGA GTCGGCCCCT CAAGTGTCAA CGTCCGCCCC TCATCTGTCA GTGAGGGCCA AGTTTTCCGC GAGGTATCCA CAACGCCGGC GGCCGCGGTG TCTCGCACAC GGCTTCGACG GCGTTTCTGG CGCGTTTGCA GGGCCATAGA CGGCCGCCAG CCCAGCGGCG AGGGCAACCA GCCCGG
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