pCMV3-LAMC1(human)
$113.00
In stock
SKU
PL63419
NM_002293.4;LAMB2
100μg liquid plasmid(Instantly):$134
| name | pCMV3-LAMC1(human) | another name | apCMV3-LAMC1(human) |
| Insert Size (bp) | 4830bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV3 | 5′ sequencing primer | CMV-F： |
| 3′ sequencing primer | BGH： | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATGAGAGGGA GCCATCGGGC CGCGCCGGCC CTGCGGCCCC GGGGGCGGCT CTGGCCCGTG CTGGCCGTGC TGGCGGCGGC CGCCGCGGCG GGCTGTGCCC AGGCAGCCAT GGACGAGTGC ACGGACGAGG GCGGGCGGCC GCAGCGCTGC ATGCCCGAGT TCGTCAACGC CGCCTTCAAC GTGACTGTGG TGGCCACCAA CACGTGTGGG ACTCCGCCCG AGGAATACTG TGTGCAGACC GGGGTGACCG GGGTCACCAA GTCCTGTCAC CTGTGCGACG CCGGGCAGCC CCACCTGCAG CACGGGGCAG CCTTCCTGAC CGACTACAAC AACCAGGCCG ACACCACCTG GTGGCAAAGC CAGACCATGC TGGCCGGGGT GCAGTACCCC AGCTCCATCA ACCTCACGCT GCACCTGGGA AAAGCTTTTG ACATCACCTA TGTGCGTCTC AAGTTCCACA CCAGCCGCCC GGAGAGCTTT GCCATTTACA AGCGCACACG GGAAGACGGG CCCTGGATTC CTTACCAGTA CTACAGTGGT TCCTGTGAGA ACACCTACTC CAAGGCAAAC CGCGGCTTCA TCAGGACAGG AGGGGACGAG CAGCAGGCCT TGTGTACTGA TGAATTCAGT GACATTTCTC CCCTCACTGG GGGCAACGTG GCCTTTTCTA CCCTGGAAGG AAGGCCCAGC GCCTATAACT TTGACAATAG CCCTGTGCTG CAGGAATGGG TAACTGCCAC TGACATCAGA GTAACTCTTA ATCGCCTGAA CACTTTTGGA GATGAAGTGT TTAACGATCC CAAAGTTCTC AAGTCCTATT ATTATGCCAT CTCTGATTTT GCTGTAGGTG GCAGATGTAA ATGTAATGGA CACGCAAGCG AGTGTATGAA GAACGAATTT GATAAGCTGG TGTGTAATTG CAAACATAAC ACATATGGAG TAGACTGTGA AAAGTGTCTT CCTTTCTTCA ATGACCGGCC GTGGAGGAGG GCAACTGCGG AAAGTGCCAG TGAATGCCTG CCCTGTGATT GCAATGGTCG ATCCCAGGAA TGCTACTTCG ACCCTGAACT CTATCGTTCC ACTGGCCATG GGGGCCACTG TACCAACTGC CAGGATAACA CAGATGGCGC CCACTGTGAG AGGTGCCGAG AGAACTTCTT CCGCCTTGGC AACAATGAAG CCTGCTCTTC ATGCCACTGT AGTCCTGTGG GCTCTCTAAG CACACAGTGT GATAGTTACG GCAGATGCAG CTGTAAGCCA GGAGTGATGG GGGACAAATG TGACCGTTGC CAGCCTGGAT TCCATTCTCT CACTGAAGCA GGATGCAGGC CATGCTCTTG TGATCCCTCT GGCAGCATAG ATGAATGTAA TATTGAAACA GGAAGATGTG TTTGCAAAGA CAATGTCGAA GGCTTCAATT GTGAAAGATG CAAACCTGGA TTTTTTAATC TGGAATCATC TAATCCTCGG GGTTGCACAC CCTGCTTCTG CTTTGGGCAT TCTTCTGTCT GTACAAACGC TGTTGGCTAC AGTGTTTATT CTATCTCCTC TACCTTTCAG ATTGATGAGG ATGGGTGGCG TGCGGAACAG AGAGATGGCT CTGAAGCATC TCTCGAGTGG TCCTCTGAGA GGCAAGATAT CGCCGTGATC TCAGACAGCT ACTTTCCTCG GTACTTCATT GCTCCTGCAA AGTTCTTGGG CAAGCAGGTG TTGAGTTATG GTCAGAACCT CTCCTTCTCC TTTCGAGTGG ACAGGCGAGA TACTCGCCTC TCTGCAGAAG ACCTTGTGCT TGAGGGAGCT GGCTTAAGAG TATCTGTACC CTTGATCGCT CAGGGCAATT CCTATCCAAG TGAGACCACT GTGAAGTATG TCTTCAGGCT CCATGAAGCA ACAGATTACC CTTGGAGGCC TGCTCTTACC CCTTTTGAAT TTCAGAAGCT CCTAAACAAC TTGACCTCTA TCAAGATACG TGGGACATAC AGTGAGAGAA GTGCTGGATA TTTGGATGAT GTCACCCTGG CAAGTGCTCG TCCTGGGCCT GGAGTCCCTG CAACTTGGGT GGAGTCCTGC ACCTGTCCTG TGGGATATGG AGGGCAGTTT TGTGAGATGT GCCTCTCAGG TTACAGAAGA GAAACTCCTA ATCTTGGACC ATACAGTCCA TGTGTGCTTT GCGCCTGCAA TGGACACAGC GAGACCTGTG ATCCTGAGAC AGGTGTTTGT AACTGCAGAG ACAATACGGC TGGCCCGCAC TGTGAGAAGT GCAGTGATGG GTACTATGGA GATTCAACTG CAGGCACCTC CTCCGATTGC CAACCCTGTC CGTGTCCTGG AGGTTCAAGT TGTGCTGTTG TTCCCAAGAC AAAGGAGGTG GTGTGCACCA ACTGTCCTAC TGGCACCACT GGTAAGAGAT GTGAGCTCTG TGATGATGGC TACTTTGGAG ACCCCCTGGG TAGAAACGGC CCTGTGAGAC TTTGCCGCCT GTGCCAGTGC AGTGACAACA TCGATCCCAA TGCAGTTGGA AATTGCAATC GCTTGACGGG AGAATGCCTG AAGTGCATCT ATAACACTGC TGGCTTCTAT TGTGACCGGT GCAAAGACGG ATTTTTTGGA AATCCCCTGG CTCCCAATCC AGCAGACAAA TGCAAAGCCT GCAATTGCAA TCTGTATGGG ACCATGAAGC AGCAGAGCAG CTGTAACCCC GTGACGGGGC AGTGTGAATG TTTGCCTCAC GTGACTGGCC AGGACTGTGG TGCTTGTGAC CCTGGATTCT ACAATCTGCA GAGTGGGCAA GGCTGTGAGA GGTGTGACTG CCATGCCTTG GGCTCCACCA ATGGGCAGTG TGACATCCGC ACCGGCCAGT GTGAGTGCCA GCCCGGCATC ACTGGTCAGC ACTGTGAGCG CTGTGAGGTC AACCACTTTG GGTTTGGACC TGAAGGCTGC AAACCCTGTG ACTGTCATCC TGAGGGATCT CTTTCACTTC AGTGCAAAGA TGATGGTCGC TGTGAATGCA GAGAAGGCTT TGTGGGAAAT CGCTGTGACC AGTGTGAAGA AAACTATTTC TACAATCGGT CTTGGCCTGG CTGCCAGGAA TGTCCAGCTT GTTACCGGCT GGTAAAGGAT AAGGTTGCTG ATCATAGAGT GAAGCTCCAG GAATTAGAGA GTCTCATAGC AAACCTTGGA ACTGGGGATG AGATGGTGAC AGATCAAGCC TTCGAGGATA GACTAAAGGA AGCAGAGAGG GAAGTTATGG ACCTCCTTCG TGAGGCCCAG GATGTCAAAG ATGTTGACCA GAATTTGATG GATCGCCTAC AGAGAGTGAA TAACACTCTG TCCAGCCAAA TTAGCCGTTT ACAGAATATC CGGAATACCA TTGAAGAGAC TGGAAACTTG GCTGAACAAG CGCGTGCCCA TGTAGAGAAC ACAGAGCGGT TGATTGAAAT CGCATCCAGA GAACTTGAGA AAGCAAAAGT CGCTGCTGCC AATGTGTCAG TCACTCAGCC AGAATCTACA GGGGACCCAA ACAACATGAC TCTTTTGGCA GAAGAGGCTC GAAAGCTTGC TGAACGTCAT AAACAGGAAG CTGATGACAT TGTTCGAGTG GCAAAGACAG CCAATGATAC GTCAACTGAG GCATACAACC TGCTTCTGAG GACACTGGCA GGAGAAAATC AAACAGCATT TGAGATTGAA GAGCTTAATA GGAAGTATGA ACAAGCGAAG AACATCTCAC AGGATCTGGA AAAACAAGCT GCCCGAGTAC ATGAGGAGGC CAAAAGGGCC GGTGACAAAG CTGTGGAGAT CTATGCCAGC GTGGCTCAGC TGAGCCCTTT GGACTCTGAG ACACTGGAGA ATGAAGCAAA TAACATAAAG ATGGAAGCTG AGAATCTGGA ACAACTGATT GACCAGAAAT TAAAAGATTA TGAGGACCTC AGAGAAGATA TGAGAGGGAA GGAACTTGAA GTCAAGAACC TTCTGGAGAA AGGCAAGACT GAACAGCAGA CCGCAGACCA ACTCCTAGCC CGAGCTGATG CTGCCAAGGC CCTCGCTGAA GAAGCTGCAA AGAAGGGACG GGATACCTTA CAAGAAGCTA ATGACATTCT CAACAACCTG AAAGATTTTG ATAGGCGTGT GAACGATAAC AAGACGGCCG CAGAGGAGGC ACTAAGGAAG ATTCCTGCCA TCAACCAGAC CATCACTGAA GCCAATGAAA AGACCAGAGA AGCCCAGCAG GCCCTGGGCA GTGCTGCGGC GGATGCCACA GAGGCCAAGA ACAAGGCCCA TGAGGCGGAG AGGATCGCGA GCGCTGTCCA AAAGAATGCC ACCAGCACCA AGGCAGAAGC TGAAAGAACT TTTGCAGAAG TTACAGATCT GGATAATGAG GTGAACAATA TGTTGAAGCA ACTGCAGGAA GCAGAAAAAG AGCTAAAGAG AAAACAAGAT GACGCTGACC AGGACATGAT GATGGCAGGG ATGGCTTCAC AGGCTGCTCA AGAAGCCGAG ATCAATGCCA GAAAAGCCAA AAACTCTGTT ACTAGCCTCC TCAGCATTAT TAATGACCTC TTGGAGCAGC TGGGGCAGCT GGATACAGTG GACCTGAATA AGCTAAACGA GATTGAAGGC ACCCTAAACA AAGCCAAAGA TGAAATGAAG GTCAGCGATC TTGATAGGAA AGTGTCTGAC CTGGAGAATG AAGCCAAGAA GCAGGAGGCT GCCATCATGG ACTATAACCG AGATATCGAG GAGATCATGA AGGACATTCG CAATCTGGAG GACATCAGGA AGACCTTACC ATCTGGCTGC TTCAACACCC CGTCCATTGA AAAGCCCTGA
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