pGEM-ABCB1(human)
$99.00
In stock
SKU
PL63417
NM_001348944.2;ABC20; CD243; CLCS; GP170; MDR1; P-GP;PGY1
100μg liquid plasmid(Instantly):$120
| name | pGEM-ABCB1(human) | another name | apGEM-ABCB1(human) |
| Insert Size (bp) | 3843bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATGGATCTTG AAGGGGACCG CAATGGAGGA GCAAAGAAGA AGAACTTTTT TAAACTGAAC AATAAAAGTG AAAAAGATAA GAAGGAAAAG AAACCAACTG TCAGTGTATT TTCAATGTTT CGCTATTCAA ATTGGCTTGA CAAGTTGTAT ATGGTGGTGG GAACTTTGGC TGCCATCATC CATGGGGCTG GACTTCCTCT CATGATGCTG GTGTTTGGAG AAATGACAGA TATCTTTGCA AATGCAGGAA ATTTAGAAGA TCTGATGTCA AACATCACTA ATAGAAGTGA TATCAATGAT ACAGGGTTCT TCATGAATCT GGAGGAAGAC ATGACCAGGT ATGCCTATTA TTACAGTGGA ATTGGTGCTG GGGTGCTGGT TGCTGCTTAC ATTCAGGTTT CATTTTGGTG CCTGGCAGCT GGAAGACAAA TACACAAAAT TAGAAAACAG TTTTTTCATG CTATAATGCG ACAGGAGATA GGCTGGTTTG ATGTGCACGA TGTTGGGGAG CTTAACACCC GACTTACAGA TGATGTCTCC AAGATTAATG AAGGAATTGG TGACAAAATT GGAATGTTCT TTCAGTCAAT GGCAACATTT TTCACTGGGT TTATAGTAGG ATTTACACGT GGTTGGAAGC TAACCCTTGT GATTTTGGCC ATCAGTCCTG TTCTTGGACT GTCAGCTGCT GTCTGGGCAA AGATACTATC TTCATTTACT GATAAAGAAC TCTTAGCGTA TGCAAAAGCT GGAGCAGTAG CTGAAGAGGT CTTGGCAGCA ATTAGAACTG TGATTGCATT TGGAGGACAA AAGAAAGAAC TTGAAAGGTA CAACAAAAAT TTAGAAGAAG CTAAAAGAAT TGGGATAAAG AAAGCTATTA CAGCCAATAT TTCTATAGGT GCTGCTTTCC TGCTGATCTA TGCATCTTAT GCTCTGGCCT TCTGGTATGG GACCACCTTG GTCCTCTCAG GGGAATATTC TATTGGACAA GTACTCACTG TATTCTTTTC TGTATTAATT GGGGCTTTTA GTGTTGGACA GGCATCTCCA AGCATTGAAG CATTTGCAAA TGCAAGAGGA GCAGCTTATG AAATCTTCAA GATAATTGAT AATAAGCCAA GTATTGACAG CTATTCGAAG AGTGGGCACA AACCAGATAA TATTAAGGGA AATTTGGAAT TCAGAAATGT TCACTTCAGT TACCCATCTC GAAAAGAAGT TAAGATCTTG AAGGGTCTGA ACCTGAAGGT GCAGAGTGGG CAGACGGTGG CCCTGGTTGG AAACAGTGGC TGTGGGAAGA GCACAACAGT CCAGCTGATG CAGAGGCTCT ATGACCCCAC AGAGGGGATG GTCAGTGTTG ATGGACAGGA TATTAGGACC ATAAATGTAA GGTTTCTACG GGAAATCATT GGTGTGGTGA GTCAGGAACC TGTATTGTTT GCCACCACGA TAGCTGAAAA CATTCGCTAT GGCCGTGAAA ATGTCACCAT GGATGAGATT GAGAAAGCTG TCAAGGAAGC CAATGCCTAT GACTTTATCA TGAAACTGCC TCATAAATTT GACACCCTGG TTGGAGAGAG AGGGGCCCAG TTGAGTGGTG GGCAGAAGCA GAGGATCGCC ATTGCACGTG CCCTGGTTCG CAACCCCAAG ATCCTCCTGC TGGATGAGGC CACGTCAGCC TTGGACACAG AAAGCGAAGC AGTGGTTCAG GTGGCTCTGG ATAAGGCCAG AAAAGGTCGG ACCACCATTG TGATAGCTCA TCGTTTGTCT ACAGTTCGTA ATGCTGACGT CATCGCTGGT TTCGATGATG GAGTCATTGT GGAGAAAGGA AATCATGATG AACTCATGAA AGAGAAAGGC ATTTACTTCA AACTTGTCAC AATGCAGACA GCAGGAAATG AAGTTGAATT AGAAAATGCA GCTGATGAAT CCAAAAGTGA AATTGATGCC TTGGAAATGT CTTCAAATGA TTCAAGATCC AGTCTAATAA GAAAAAGATC AACTCGTAGG AGTGTCCGTG GATCACAAGC CCAAGACAGA AAGCTTAGTA CCAAAGAGGC TCTGGATGAA AGTATACCTC CAGTTTCCTT TTGGAGGATT ATGAAGCTAA ATTTAACTGA ATGGCCTTAT TTTGTTGTTG GTGTATTTTG TGCCATTATA AATGGAGGCC TGCAACCAGC ATTTGCAATA ATATTTTCAA AGATTATAGG GGTTTTTACA AGAATTGATG ATCCTGAAAC AAAACGACAG AATAGTAACT TGTTTTCACT ATTGTTTCTA GCCCTTGGAA TTATTTCTTT TATTACATTT TTCCTTCAGG GTTTCACATT TGGCAAAGCT GGAGAGATCC TCACCAAGCG GCTCCGATAC ATGGTTTTCC GATCCATGCT CAGACAGGAT GTGAGTTGGT TTGATGACCC TAAAAACACC ACTGGAGCAT TGACTACCAG GCTCGCCAAT GATGCTGCTC AAGTTAAAGG GGCTATAGGT TCCAGGCTTG CTGTAATTAC CCAGAATATA GCAAATCTTG GGACAGGAAT AATTATATCC TTCATCTATG GTTGGCAACT AACACTGTTA CTCTTAGCAA TTGTACCCAT CATTGCAATA GCAGGAGTTG TTGAAATGAA AATGTTGTCT GGACAAGCAC TGAAAGATAA GAAAGAACTA GAAGGTTCTG GGAAGATCGC TACTGAAGCA ATAGAAAACT TCCGAACCGT TGTTTCTTTG ACTCAGGAGC AGAAGTTTGA ACATATGTAT GCTCAGAGTT TGCAGGTACC ATACAGAAAC TCTTTGAGGA AAGCACACAT CTTTGGAATT ACATTTTCCT TCACCCAGGC AATGATGTAT TTTTCCTATG CTGGATGTTT CCGGTTTGGA GCCTACTTGG TGGCACATAA ACTCATGAGC TTTGAGGATG TTCTGTTAGT ATTTTCAGCT GTTGTCTTTG GTGCCATGGC CGTGGGGCAA GTCAGTTCAT TTGCTCCTGA CTATGCCAAA GCCAAAATAT CAGCAGCCCA CATCATCATG ATCATTGAAA AAACCCCTTT GATTGACAGC TACAGCACGG AAGGCCTAAT GCCGAACACA TTGGAAGGAA ATGTCACATT TGGTGAAGTT GTATTCAACT ATCCCACCCG ACCGGACATC CCAGTGCTTC AGGGACTGAG CCTGGAGGTG AAGAAGGGCC AGACGCTGGC TCTGGTGGGC AGCAGTGGCT GTGGGAAGAG CACAGTGGTC CAGCTCCTGG AGCGGTTCTA CGACCCCTTG GCAGGGAAAG TGCTGCTTGA TGGCAAAGAA ATAAAGCGAC TGAATGTTCA GTGGCTCCGA GCACACCTGG GCATCGTGTC CCAGGAGCCC ATCCTGTTTG ACTGCAGCAT TGCTGAGAAC ATTGCCTATG GAGACAACAG CCGGGTGGTG TCACAGGAAG AGATCGTGAG GGCAGCAAAG GAGGCCAACA TACATGCCTT CATCGAGTCA CTGCCTAATA AATATAGCAC TAAAGTAGGA GACAAAGGAA CTCAGCTCTC TGGTGGCCAG AAACAACGCA TTGCCATAGC TCGTGCCCTT GTTAGACAGC CTCATATTTT GCTTTTGGAT GAAGCCACGT CAGCTCTGGA TACAGAAAGT GAAAAGGTTG TCCAAGAAGC CCTGGACAAA GCCAGAGAAG GCCGCACCTG CATTGTGATT GCTCACCGCC TGTCCACCAT CCAGAATGCA GACTTAATAG TGGTGTTTCA GAATGGCAGA GTCAAGGAGC ATGGCACGCA TCAGCAGCTG CTGGCACAGA AAGGCATCTA TTTTTCAATG GTCAGTGTCC AGGCTGGAAC AAAGCGCCAG TGA
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