pGEM-NSD2(human)
$113.00
In stock
SKU
PL63412
NM_133330.2;KMT3F; KMT3G; MMSET; REIIBP; TRX5; WHS;WHSC1
100μg liquid plasmid(Instantly):$134
| name | pGEM-NSD2(human) | another name | apGEM-NSD2(human) |
| Insert Size (bp) | 4098bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATGGAATTTA GCATCAAGCA GAGTCCCCTT TCTGTTCAGA GTGTTGTAAA GTGCATAAAG ATGAAGCAGG CACCAGAAAT CCTCGGCAGT GCCAACGGGA AGACTCCGAG CTGCGAGGTG AACCGCGAGT GTTCTGTGTT CCTCAGCAAA GCCCAGCTCT CCAGTAGCCT GCAGGAGGGG GTCATGCAGA AGTTTAACGG CCACGACGCC CTGCCCTTTA TTCCAGCCGA CAAGCTGAAA GATCTTACTT CCCGGGTGTT TAATGGAGAA CCCGGCGCAC ACGATGCCAA ACTGCGTTTT GAGTCCCAGG AAATGAAAGG GATTGGGACA CCCCCTAACA CTACCCCTAT CAAAAATGGC TCTCCAGAAA TTAAGCTGAA AATCACCAAA ACATACATGA ATGGGAAGCC TCTCTTTGAA TCTTCCATTT GTGGTGACAG TGCTGCTGAT GTGTCTCAGT CAGAAGAAAA TGGACAAAAA CCAGAAAACA AGGCGAGAAG GAACAGGAAG AGGAGCATAA AATATGACTC CTTGCTGGAG CAGGGCCTTG TCGAAGCAGC TCTTGTGTCT AAGATCTCAA GTCCTTCAGA TAAAAAGATT CCAGCTAAGA AAGAGTCTTG TCCAAACACT GGAAGAGACA AAGACCACCT GTTGAAATAC AACGTTGGTG ATTTGGTGTG GTCCAAAGTG TCGGGTTACC CTTGGTGGCC TTGCATGGTT TCTGCAGATC CACTCCTTCA CAGCTATACC AAACTTAAAG GTCAGAAAAA GAGTGCACGC CAGTATCACG TACAGTTCTT TGGTGACGCC CCAGAAAGAG CTTGGATATT TGAGAAGAGC CTCGTAGCTT TTGAAGGAGA AGGACAGTTT GAAAAATTAT GCCAGGAAAG TGCCAAGCAG GCACCCACGA AAGCTGAGAA AATTAAGCTA TTGAAACCAA TTTCAGGGAA ATTGAGGGCC CAGTGGGAAA TGGGCATTGT TCAAGCAGAA GAAGCTGCAA GCATGTCAGT GGAGGAGCGG AAAGCCAAGT TCACCTTTCT CTATGTGGGG GACCAGCTTC ATCTCAACCC TCAAGTAGCC AAGGAGGCTG GCATTGCTGC AGAGTCTTTG GGAGAAATGG CAGAATCCTC AGGAGTCAGT GAAGAAGCTG CTGAAAACCC CAAGTCTGTG AGAGAAGAGT GCATTCCCAT GAAGAGAAGG CGGAGGGCCA AACTGTGTAG CTCTGCAGAG ACCCTGGAGA GTCACCCCGA CATAGGGAAG AGTACTCCTC AAAAGACGGC AGAGGCTGAC CCCAGAAGAG GAGTAGGGTC TCCTCCTGGG AGGAAGAAGA CCACAGTCTC CATGCCACGA AGCAGGAAGG GAGATGCAGC ATCCCAGTTT TTGGTCTTCT GTCAAAAACA CAGGGATGAG GTGGTAGCTG AGCACCCAGA TGCTTCAGGT GAGGAGATTG AAGAGCTGCT CAGGTCACAG TGGAGTCTGC TGAGTGAGAA GCAGAGAGCA CGCTACAACA CCAAGTTTGC CCTGGTGGCC CCTGTCCAGG CTGAAGAAGA CTCTGGTAAT GTAAATGGGA AAAAAAGAAA CCACACAAAG AGGATACAGG ACCCTACAGA AGATGCTGAA GCTGAGGACA CACCCAGGAA AAGACTCAGG ACGGACAAGC ACAGTCTTCG GAAGAGAGAC ACAATCACTG ACAAAACGGC CAGAACAAGC TCTTACAAGG CCATGGAGGC AGCCTCCTCG CTCAAGAGCC AGGCAGCAAC GAAAAATCTG TCTGATGCAT GTAAACCACT GAAGAAGCGA AATCGGGCTT CCACGGCAGC ATCTTCAGCT CTTGGGTTTA GCAAAAGTTC ATCTCCTTCT GCATCCTTAA CTGAGAATGA GGTCTCGGAC AGCCCGGGAG ACGAGCCCTC GGAGTCCCCA TACGAAAGTG CAGACGAAAC ACAAACTGAA GTATCTGTCT CATCCAAAAA GTCTGAGCGA GGAGTGACTG CCAAAAAGGA GTATGTGTGC CAGCTGTGTG AGAAGCCGGG CAGCCTCCTG CTCTGTGAAG GACCCTGCTG CGGAGCTTTC CACCTCGCCT GCCTTGGGCT TTCCCGGAGG CCAGAAGGGA GGTTCACCTG CAGCGAGTGT GCCTCAGGGA TTCACTCATG TTTCGTGTGT AAAGAGAGCA AGACAGATGT TAAGCGCTGT GTGGTAACTC AGTGTGGAAA ATTTTACCAT GAGGCTTGTG TGAAAAAATA CCCTCTGACT GTATTTGAGA GCCGAGGTTT CCGCTGCCCC CTCCACAGCT GTGTGAGCTG CCATGCTTCC AACCCTTCAA ACCCAAGGCC GTCAAAAGGT AAAATGATGC GGTGTGTCCG CTGCCCCGTT GCCTATCACA GCGGGGATGC TTGTCTGGCA GCAGGATGCT CAGTGATCGC CTCCAACAGC ATCATCTGCA CTGCCCACTT CACTGCTCGG AAGGGGAAGC GACACCACGC CCACGTCAAC GTGAGCTGGT GCTTCGTGTG CTCCAAAGGG GGGAGCCTTC TGTGCTGTGA GTCCTGCCCA GCGGCCTTCC ACCCTGACTG CCTGAACATC GAGATGCCTG ACGGCAGCTG GTTCTGCAAT GACTGCAGGG CTGGGAAGAA GCTGCACTTC CAGGATATCA TTTGGGTGAA ACTTGGGAAC TACAGATGGT GGCCGGCAGA AGTTTGCCAT CCCAAAAATG TTCCCCCAAA TATTCAGAAA ATGAAGCACG AGATTGGAGA ATTCCCTGTG TTTTTCTTTG GGTCTAAAGA TTATTACTGG ACGCATCAGG CGCGAGTGTT CCCGTACATG GAGGGGGACC GGGGCAGCCG CTACCAGGGG GTCAGAGGGA TCGGAAGAGT CTTCAAAAAC GCACTGCAAG AAGCTGAAGC TCGTTTTCGT GAAATTAAGC TTCAGAGGGA AGCCCGAGAA ACACAGGAGA GCGAGCGCAA GCCCCCACCA TACAAGCACA TCAAGGTGAA TAAGCCTTAC GGGAAAGTCC AGATCTACAC AGCGGATATT TCAGAAATCC CTAAGTGCAA CTGCAAGCCC ACAGATGAGA ATCCTTGTGG CTTTGATTCG GAGTGTCTGA ACAGGATGCT GATGTTTGAG TGCCACCCGC AGGTGTGTCC CGCGGGCGAG TTCTGCCAGA ACCAGTGCTT CACCAAGCGC CAGTACCCAG AGACCAAGAT CATCAAGACA GATGGCAAAG GGTGGGGCCT GGTCGCCAAG AGGGACATCA GAAAGGGAGA ATTTGTTAAC GAGTACGTTG GGGAGCTGAT CGACGAGGAG GAGTGCATGG CGAGAATCAA GCACGCACAC GAGAACGACA TCACCCACTT CTACATGCTC ACTATAGACA AGGACCGTAT AATAGACGCT GGCCCCAAAG GAAACTACTC TCGATTTATG AATCACAGCT GCCAGCCCAA CTGTGAGACC CTCAAGTGGA CAGTGAATGG GGACACTCGT GTGGGCCTGT TTGCCGTCTG TGACATTCCT GCAGGGACGG AGCTGACTTT TAACTACAAC CTCGATTGTC TGGGCAATGA AAAAACGGTC TGCCGGTGTG GAGCCTCCAA TTGCAGTGGA TTCCTCGGGG ATAGACCAAA GACCTCGACG ACCCTTTCAT CAGAGGAAAA GGGCAAAAAG ACCAAGAAGA AAACGAGGCG GCGCAGAGCA AAAGGGGAAG GGAAGAGGCA GTCAGAGGAC GAGTGCTTCC GCTGCGGTGA TGGCGGGCAG CTGGTGCTGT GTGACCGCAA GTTCTGCACC AAGGCCTACC ACCTGTCCTG CCTGGGCCTT GGCAAGCGGC CCTTCGGGAA GTGGGAATGT CCTTGGCATC ATTGTGACGT GTGTGGCAAA CCTTCGACTT CATTTTGCCA CCTCTGCCCC AATTCGTTCT GTAAGGAGCA CCAGGACGGG ACAGCCTTCA GCTGCACCCC GGACGGGCGG TCCTACTGCT GTGAGCATGA CTTAGGGGCG GCATCGGTCA GAAGCACCAA GACTGAGAAG CCCCCCCCAG AGCCAGGGAA GCCGAAGGGG AAGAGGCGGC GGCGGAGGGG CTGGCGGAGA GTCACAGAGG GCAAATAG
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