NM_002473.6;BDPLT6; DFNA17; EPSTS; FTNS; MATINS; MHA;NMHC-II-A; NMMHC-IIA; NMMHCA
100μg liquid plasmid(Instantly):$163
| name | pCMV7.1-3×FLAG-MYH9(human)-Myc-6×His | another name | apCMV7.1-3×FLAG-MYH9(human)-Myc-6×His |
| Insert Size (bp) | 5883bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA TTACAAGGAT GACGATGACA AGCTTGCGGC CGCTATGGCA CAGCAAGCTG CCGATAAGTA TCTCTATGTG GATAAAAACT TCATCAACAA TCCGCTGGCC CAGGCCGACT GGGCTGCCAA GAAGCTGGTA TGGGTGCCTT CCGACAAGAG TGGCTTTGAG CCAGCCAGCC TCAAGGAGGA GGTGGGCGAA GAGGCCATCG TGGAGCTGGT GGAGAATGGG AAGAAGGTGA AGGTGAACAA GGATGACATC CAGAAGATGA ACCCGCCCAA GTTCTCCAAG GTGGAGGACA TGGCAGAGCT CACGTGCCTC AACGAAGCCT CGGTGCTGCA CAACCTCAAG GAGCGTTACT ACTCAGGGCT CATCTACACC TATTCAGGCC TGTTCTGTGT GGTCATCAAT CCTTACAAGA ACCTGCCCAT CTACTCTGAA GAGATTGTGG AAATGTACAA GGGCAAGAAG AGGCACGAGA TGCCCCCTCA CATCTATGCC ATCACAGACA CCGCCTACAG GAGTATGATG CAAGACCGAG AAGATCAATC CATCTTGTGC ACTGGTGAAT CTGGAGCTGG CAAGACGGAG AACACCAAGA AGGTCATCCA GTATCTGGCG TACGTGGCGT CCTCGCACAA GAGCAAGAAG GACCAGGGCG AGCTGGAGCG GCAGCTGCTG CAGGCCAACC CCATCCTGGA GGCCTTCGGG AACGCCAAGA CCGTGAAGAA TGACAACTCC TCCCGCTTCG GCAAATTCAT TCGCATCAAC TTTGATGTCA ATGGCTACAT TGTTGGAGCC AACATTGAGA CTTATCTTTT GGAGAAATCT CGTGCTATCC GCCAAGCCAA GGAAGAACGG ACCTTCCACA TCTTCTATTA TCTCCTGTCT GGGGCTGGAG AGCACCTGAA GACCGATCTC CTGTTGGAGC CGTACAACAA ATACCGCTTC CTGTCCAATG GACACGTCAC CATCCCCGGG CAGCAGGACA AGGACATGTT CCAGGAGACC ATGGAGGCCA TGAGGATTAT GGGCATCCCA GAAGAGGAGC AAATGGGCCT GCTGCGGGTC ATCTCAGGGG TTCTTCAGCT CGGCAACATC GTCTTCAAGA AGGAGCGGAA CACTGACCAG GCGTCCATGC CCGACAACAC AGCTGCCCAA AAGGTGTCCC ATCTCTTGGG TATCAATGTG ACCGATTTCA CCAGAGGAAT CCTCACCCCG CGCATCAAGG TGGGACGGGA TTACGTCCAG AAGGCGCAGA CTAAAGAGCA GGCTGACTTT GCCATCGAGG CCTTGGCCAA GGCGACCTAT GAGCGGATGT TCCGCTGGCT GGTGCTGCGC ATCAACAAGG CTCTGGACAA GACCAAGAGG CAGGGCGCCT CCTTCATCGG GATCCTGGAC ATTGCCGGCT TCGAGATCTT TGATCTGAAC TCGTTTGAGC AGCTGTGCAT CAATTACACC AATGAGAAGC TGCAGCAGCT CTTCAACCAC ACCATGTTCA TCCTGGAGCA GGAGGAGTAC CAGCGCGAGG GCATCGAGTG GAACTTCATC GACTTTGGCC TCGACCTGCA GCCCTGCATC GACCTCATTG AGAAGCCAGC AGGCCCCCCG GGCATTCTGG CCCTGCTGGA CGAGGAGTGC TGGTTCCCCA AAGCCACCGA CAAGAGCTTC GTGGAGAAGG TGATGCAGGA GCAGGGCACC CACCCCAAGT TCCAGAAGCC CAAGCAGCTG AAGGACAAAG CTGATTTCTG CATTATCCAC TATGCCGGCA AGGTGGATTA CAAAGCTGAC GAGTGGCTGA TGAAGAACAT GGATCCCCTG AATGACAACA TCGCCACACT GCTCCACCAG TCCTCTGACA AGTTTGTCTC GGAGCTGTGG AAGGATGTGG ACCGCATCAT CGGCCTGGAC CAGGTGGCCG GCATGTCGGA GACCGCACTG CCCGGGGCCT TCAAGACGCG GAAGGGCATG TTCCGCACTG TGGGGCAGCT TTACAAGGAG CAGCTGGCCA AGCTGATGGC TACGCTGAGG AACACGAACC CCAACTTTGT CCGCTGCATC ATCCCCAACC ACGAGAAGAA GGCCGGCAAG CTGGACCCGC ATCTCGTGCT GGACCAGCTG CGCTGCAACG GTGTTCTCGA GGGCATCCGT ATCTGCCGCC AGGGCTTCCC CAACAGGGTG GTCTTCCAGG AGTTTCGGCA GAGATATGAG ATCCTGACTC CAAACTCCAT TCCCAAGGGT TTCATGGACG GGAAGCAGGC GTGCGTGCTC ATGATAAAAG CCCTGGAGCT CGACAGCAAT CTGTACCGCA TTGGCCAGAG CAAAGTCTTC TTCCGTGCCG GTGTGCTGGC CCACCTGGAG GAGGAGCGAG ACCTGAAGAT CACCGACGTC ATCATAGGGT TCCAGGCCTG CTGCAGGGGC TACCTGGCCA GGAAAGCATT TGCCAAGCGG CAGCAGCAGC TTACCGCCAT GAAGGTCCTC CAGCGGAACT GCGCTGCCTA CCTGAAGCTG CGGAACTGGC AGTGGTGGCG GCTCTTCACC AAGGTCAAGC CGCTGCTGCA GGTGAGCCGG CAGGAGGAGG AGATGATGGC CAAGGAGGAG GAGCTGGTGA AGGTCAGAGA GAAGCAGCTG GCTGCGGAGA ACAGGCTCAC GGAGATGGAG ACGCTGCAGT CTCAGCTCAT GGCAGAGAAA TTGCAGCTGC AGGAGCAGCT CCAGGCAGAA ACCGAGCTGT GTGCCGAGGC TGAGGAGCTC CGGGCCCGCC TGACCGCCAA GAAGCAGGAA TTAGAAGAGA TCTGCCATGA CCTAGAGGCC AGGGTGGAGG AGGAGGAGGA GCGCTGCCAG CACCTGCAGG CGGAGAAGAA GAAGATGCAG CAGAACATCC AGGAGCTTGA GGAGCAGCTG GAGGAGGAGG AGAGCGCCCG GCAGAAGCTG CAGCTGGAGA AGGTGACCAC CGAGGCGAAG CTGAAAAAGC TGGAGGAGGA GCAGATCATC CTGGAGGACC AGAACTGCAA GCTGGCCAAG GAAAAGAAAC TGCTGGAAGA CAGAATAGCT GAGTTCACCA CCAACCTCAC AGAAGAGGAG GAGAAATCTA AGAGCCTCGC CAAGCTCAAG AACAAGCATG AGGCAATGAT CACTGACTTG GAAGAGCGCC TCCGCAGGGA GGAGAAGCAG CGACAGGAGC TGGAGAAGAC CCGCCGGAAG CTGGAGGGAG ACTCCACAGA CCTCAGCGAC CAGATCGCCG AGCTCCAGGC CCAGATCGCG GAGCTCAAGA TGCAGCTGGC CAAGAAAGAG GAGGAGCTCC AGGCCGCCCT GGCCAGAGTG GAAGAGGAAG CTGCCCAGAA GAACATGGCC CTCAAGAAGA TCCGGGAGCT GGAATCTCAG ATCTCTGAAC TCCAGGAAGA CCTGGAGTCT GAGCGTGCTT CCAGGAATAA AGCTGAGAAG CAGAAACGGG ACCTTGGGGA AGAGCTAGAG GCTCTGAAAA CAGAGTTGGA GGACACGCTG GATTCCACAG CTGCCCAGCA GGAGCTCAGG TCAAAACGTG AGCAGGAGGT GAACATCCTG AAGAAGACCC TGGAGGAGGA GGCCAAGACC CACGAGGCCC AGATCCAGGA GATGAGGCAG AAGCACTCAC AGGCCGTGGA GGAGCTGGCG GAGCAGCTGG AGCAGACGAA GCGGGTGAAA GCAAACCTCG AGAAGGCAAA GCAGACTCTG GAGAACGAGC GGGGGGAGCT GGCCAACGAG GTGAAGGTGC TGCTGCAGGG CAAAGGGGAC TCGGAGCACA AGCGCAAGAA AGTGGAGGCG CAGCTGCAGG AGCTGCAGGT CAAGTTCAAC GAGGGAGAGC GCGTGCGCAC AGAGCTGGCC GACAAGGTCA CCAAGCTGCA GGTGGAGCTG GACAACGTGA CCGGGCTTCT CAGCCAGTCC GACAGCAAGT CCAGCAAGCT CACCAAGGAC TTCTCCGCGC TGGAGTCCCA GCTGCAGGAC ACTCAGGAGC TGCTGCAGGA GGAGAACCGG CAGAAGCTGA GCCTGAGCAC CAAGCTCAAG CAGGTGGAGG ACGAGAAGAA TTCCTTCCGG GAGCAGCTGG AGGAGGAGGA GGAGGCCAAG CACAACCTGG AGAAGCAGAT CGCCACCCTC CATGCCCAGG TGGCCGACAT GAAAAAGAAG ATGGAGGACA GTGTGGGGTG CCTGGAAACT GCTGAGGAGG TGAAGAGGAA GCTCCAGAAG GACCTGGAGG GCCTGAGCCA GCGGCACGAG GAGAAGGTGG CCGCCTACGA CAAGCTGGAG AAGACCAAGA CGCGGCTGCA GCAGGAGCTG GACGACCTGC TGGTGGACCT GGACCACCAG CGCCAGAGCG CGTGCAACCT GGAGAAGAAG CAGAAGAAGT TTGACCAGCT CCTGGCGGAG GAGAAGACCA TCTCTGCCAA GTATGCAGAG GAGCGCGACC GGGCTGAGGC GGAGGCCCGA GAGAAGGAGA CCAAGGCTCT GTCGCTGGCC CGGGCCCTGG AGGAAGCCAT GGAGCAGAAG GCGGAGCTGG AGCGGCTCAA CAAGCAGTTC CGCACGGAGA TGGAGGACCT TATGAGCTCC AAGGATGATG TGGGCAAGAG TGTCCACGAG CTGGAGAAGT CCAAGCGGGC CCTAGAGCAG CAGGTGGAGG AGATGAAGAC GCAGCTGGAA GAGCTGGAGG ACGAGCTGCA GGCCACCGAA GATGCCAAGC TGCGGTTGGA GGTCAACCTG CAGGCCATGA AGGCCCAGTT CGAGCGGGAC CTGCAGGGCC GGGACGAGCA GAGCGAGGAG AAGAAGAAGC AGCTGGTCAG ACAGGTGCGG GAGATGGAGG CAGAGCTGGA GGACGAGAGG AAGCAGCGCT CGATGGCAGT GGCCGCCCGG AAGAAGCTGG AGATGGACCT GAAGGACCTG GAGGCGCACA TCGACTCGGC CAACAAGAAC CGGGACGAAG CCATCAAACA GCTGCGGAAG CTGCAGGCCC AGATGAAGGA CTGCATGCGC GAGCTGGATG ACACCCGCGC CTCTCGTGAG GAGATCCTGG CCCAGGCCAA AGAGAACGAG AAGAAGCTGA AGAGCATGGA GGCCGAGATG ATCCAGTTGC AGGAGGAACT GGCAGCCGCG GAGCGTGCCA AGCGCCAGGC CCAGCAGGAG CGGGATGAGC TGGCTGACGA GATCGCCAAC AGCAGCGGCA AAGGAGCCCT GGCGTTAGAG GAGAAGCGGC GTCTGGAGGC CCGCATCGCC CAGCTGGAGG AGGAGCTGGA GGAGGAGCAG GGCAACACGG AGCTGATCAA CGACCGGCTG AAGAAGGCCA ACCTGCAGAT CGACCAGATC AACACCGACC TGAACCTGGA GCGCAGCCAC GCCCAGAAGA ACGAGAATGC TCGGCAGCAG CTGGAACGCC AGAACAAGGA GCTTAAGGTC AAGCTGCAGG AGATGGAGGG CACTGTCAAG TCCAAGTACA AGGCCTCCAT CACCGCCCTC GAGGCCAAGA TTGCACAGCT GGAGGAGCAG CTGGACAACG AGACCAAGGA GCGCCAGGCA GCCTGCAAAC AGGTGCGTCG GACCGAGAAG AAGCTGAAGG ATGTGCTGCT GCAGGTGGAT GACGAGCGGA GGAACGCCGA GCAGTACAAG GACCAGGCCG ACAAGGCATC TACCCGCCTG AAGCAGCTCA AGCGGCAGCT GGAGGAGGCC GAAGAGGAGG CCCAGCGGGC CAACGCCTCC CGCCGGAAAC TGCAGCGCGA GCTGGAGGAC GCCACTGAGA CGGCCGATGC CATGAACCGC GAAGTCAGCT CCCTAAAGAA CAAGCTCAGG CGCGGGGACC TGCCGTTTGT CGTGCCCCGC CGAATGGCCC GGAAAGGCGC CGGGGATGGC TCCGACGAAG AGGTAGATGG CAAAGCGGAT GGGGCTGAGG CCAAACCTGC CGAAGAGCAA AAGCTCATTT CTGAAGAGGA CTTGCACCAT CACCATCACC ATTAGGGTAC CAGTCGACTC TAGAGGATCC CGGGT
