pLIVE
$99.00
In stock
SKU
PL63375
100μg liquid plasmid(Instantly):$120
| name | pLIVE | another name | apLIVE |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | Albumin | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTAATTAAGA TCTTTTTGAT GGCAGAGTTC AGTTTACCGG GTCACATTGT ACCTGGGAAG ATTCAAGGAT TTATGGAAAA AGTCAACAAC AGGAGTCAGA GCAGCCGGAA AAGCATGGAC TCTGTACTTA GGACTGCGCT TTGAGCAATG GCACAGCAAG CTTTAACCCT GTTTGCAGTC AGCACACAAA CTGTGGTTCA AAGCTCCACT TTATCTCTTC TTGTGGAATT CAGATATCAG ATCAGTTTAA ACCTTGCGGC CGCACTAGTG CTCAAATGGG AGACAAAGAG ATTAAGCTCT TATGTAAAAT TTGCTGTTTT ACATAACTTT AATGAATGGA CAAAGTCTTG TGCATGGGGG TGGGGGTGGG GTTAGAGGGG AACAGCTCCA GATGGCAAAC ATACGCAAGG GATTTAGTCA AACAACTTTT TGGCAAAGAT GGTATGATTT TGTAATGGGG TAGGAACCAA TGAAATGCGA GGTAAGTATG GTTAATAATC TACAGTTATT GGTTAAAGAA GTATATTAGA GCGAGTCTTT CTGCACACAG ATCACCTTCC TATCAACCCC ACTAGCCTCT GGCAAAGGTA CCAGTGTACA GGTTTGTTTC CTTTTTTAAA ATACATTGAG TATGCTTGCC TTTTAGATAT AGAAATATCT GATGCTGTCT TCTTCACTAA ATTTTGATTA CATGATTTGA CAGCAATATT GAAGAGTCTA ACAGCCAGCA CGCAGGTTGG TAAGTACTGT GGGAACATCA CAGATTTTGG CTCCATGCCC TAAAGAGAAA TTGGCTTTCA GATTATTTGG ATTAAAAACA AAGACTTTCT TAAGAGATGT AAAATTTTCA TGATGTTTTC TTTTTTGCTA AAACTAAAGA ATTATTCTTT TACATTTCAG TTTTTCTGCT AGCAGGCGCG CCAGTCGACT CCCGGGATCC AGAGCTCACC GCGGACTCGA GTAACATCAC ATTTAAAAGC ATCTCAGGTA ACTATATTTT GAATTTTTTA AAAAAGTAAC TGTAATAGTT ATTATTAAAA TAGCAAAGAT TGACCATTTC CAAGAGCCAT ATAGACCAGC ACCGACCACT ATTCTAAACT ATTTATGTAT GTAAATATTA GCTTTTAAAA TTCTCAAAAT AGTTGCTGAG TTGGGAACCA CTATTATTTC TATCGATTCA GCAGCCGTAA GTCTAGGACA GGCTTAAATT GTTTTCACTG GTGTAAATTG CAGAAAGATG ATCTAAGTAA TTTGGCATTT ATTTTAATAG GTTTGAAAAA CACATGCCAT TTTACAAATA AGACTTATAT TTGTCCTTTT GTTTTTCAGC CTACCATGAG AATAAGAGAA AGAAAATGAA GATCAAAAGC TTATTCATCT GTTTTTCTTT TTCGTTGGTG TAAAGCCAAC ACCCTGTCTA AAAAACATAA ATTTCTTTAA TCATTTTGCC TCTTTTCTCT GTGCTTCAAT TAATAAAAAA TGGAAAGAAT CTAATAGAGT GGTACAGCAC TGTTATTTTT CAAAGATGTG TTGCTATCCT GAAAATTCTG TAGGTTCTGT GGAAGTTCCA GTGTTCTCTC TTATTCCACT TCGGTAGAGG ATTTCTAGTT TCTTGTGGGC TAATTAAATA AATCATTAAT ACTCTTCTAA GTTATGGATT ATAAACATTC AAAATAATAT TTTGACATTA TGATAATTCT GAATAAAAGA ACAAAAACCA TGGTATAGGT AAGGAATATA AAACATGGCT TTTACCTTAG AAAAAACAAT TCTAAAATTC ATATGGAATC AAAAAAGAGC CTGCAGGCGG AATTGCCAGC TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC CTGCAAATGA CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGTCTAGA GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGGCTCGA CAGATCGCGG CCGCAAGAGA TCA
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