pBIND-TRa
$99.00
In stock
SKU
PL63329
100μg liquid plasmid(Instantly):$120
| name | pBIND-TRa | another name | apBIND-TRa |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | hybridization | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Neo/G418 | Fluorescent labeling | Rluc |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG GGCTAGCCAG CTTGAAGCAA GCCTCCTGAA AGATGAAGCT ACTGTCTTCT ATCGAACAAG CATGCGATAT TTGCCGACTT AAAAAGCTCA AGTGCTCCAA AGAAAAACCG AAGTGCGCCA AGTGTCTGAA GAACAACTGG GAGTGTCGCT ACTCTCCCAA AACCAAAAGG TCTCCGCTGA CTAGGGCACA TCTGACAGAA GTGGAATCAA GGCTAGAAAG ACTGGAACAG CTATTTCTAC TGATTTTTCC TCGAGAAGAC CTTGACATGA TTTTGAAAAT GGATTCTTTA CAGGATATAA AAGCATTGTT AACAGGATTA TTTGTACAAG ATAATGTGAA TAAAGATGCC GTCACAGATA GATTGGCTTC AGTGGAGACT GATATGCCTC TAACATTGAG ACAGCATAGA ATAAGTGCGA CATCATCATC GGAAGAGAGT AGTAACAAAG GTCAAAGACA GTTGACTGTA GCGATTCCGT CGACACCACC TACTCCCTCT CCAGCGATCG CCGAAGAGTG GGATCTGATC CACATTGCCA CAGAGGCCCA TCGCAGCACC AATGCCCAGG GCAGCCATTG GAAACAGAGG CGGAAATTCC TGCCCGATGA CATTGGCCAG TCACCCATTG TCTCCATGCC GGACGGAGAC AAGGTGGACC TGGAAGCCTT CAGCGAGTTT ACCAAGATCA TCACCCCGGC CATCACCCGT GTGGTGGACT TTGCCAAAAA ACTGCCCATG TTCTCCGAGC TGCCTTGCGA AGACCAGATC ATCCTCCTGA AGGGGTGCTG CATGGAGATC ATGTCCCTGC GGGCGGCTGT CCGCTACGAC CCTGAGAGCG ACACCCTGAC GCTGAGTGGG GAGATGGCTG TCAAGCGGGA GCAGCTCAAG AATGGCGGCC TGGGCGTAGT CTCCGACGCC ATCTTTGAAC TGGGCAAGTC ACTCTCTGCC TTTAACCTGG ATGACACGGA AGTGGCTCTG CTGCAGGCTG TGCTGCTAAT GTCAACAGAC CGCTCGGGCC TGCTGTGTGT GGACAAGATC GAGAAGAGTC AGGAGGCGTA CCTGCTGGCG TTCGAGCACT ACGTCAACCA CCGCAAACAC AACATTCCGC ACTTCTGGCC CAAGCTGCTG ATGAAGGAGA GAGAAGTGCA GAGTTCGATT CTGTACAAGG GGGCAGCGGC AGAAGGCCGG CCGGGCGGGT CACTGGGCGT CCACCCGGAA GGACAGCAGC TTCTCGGAAT GCATGTTGTT TAAACGAATT CGGGCTCGGT ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCGGCCGC TTCGAGCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGATGTGGGA GGTTTTTTTA AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGAATTTT AACAAAATAT TAACGCTTAC AATTTCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATACGC GGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACCAAAGGCT GGAGCCACCA TGGCTTCCAA GGTGTACGAC CCCGAGCAAC GCAAACGCAT GATCACTGGG CCTCAGTGGT GGGCTCGCTG CAAGCAAATG AACGTGCTGG ACTCCTTCAT CAACTACTAT GATTCCGAGA AGCACGCCGA GAACGCCGTG ATTTTTCTGC ATGGTAACGC TGCCTCCAGC TACCTGTGGA GGCACGTCGT GCCTCACATC GAGCCCGTGG CTAGATGCAT CATCCCTGAT CTGATCGGAA TGGGTAAGTC CGGCAAGAGC GGGAATGGCT CATATCGCCT CCTGGATCAC TACAAGTACC TCACCGCTTG GTTCGAGCTG CTGAACCTTC CAAAGAAAAT CATCTTTGTG GGCCACGACT GGGGGGCTTG TCTGGCCTTT CACTACTCCT ACGAGCACCA AGACAAGATC AAGGCCATCG TCCATGCTGA GAGTGTCGTG GACGTGATCG AGTCCTGGGA CGAGTGGCCT GACATCGAGG AGGATATCGC CCTGATCAAG AGCGAAGAGG GCGAGAAAAT GGTGCTTGAG AATAACTTCT TCGTCGAGAC CATGCTCCCA AGCAAGATCA TGCGGAAACT GGAGCCTGAG GAGTTCGCTG CCTACCTGGA GCCATTCAAG GAGAAGGGCG AGGTTAGACG GCCTACCCTC TCCTGGCCTC GCGAGATCCC TCTCGTTAAG GGAGGCAAGC CCGACGTCGT CCAGATTGTC CGCAACTACA ACGCCTACCT TCGGGCCAGC GACGATCTGC CTAAGATGTT CATCGAGTCC GACCCTGGGT TCTTTTCCAA CGCTATTGTC GAGGGAGCTA AGAAGTTCCC TAACACCGAG TTCGTGAAGG TGAAGGGCCT CCACTTCAGC CAGGAGGACG CTCCAGATGA AATGGGTAAG TACATCAAGA GCTTCGTGGA GCGCGTGCTG AAGAACGAGC AGACCGGTGG TGGGAGCGGA GGTGGCGGAT CAGGTGGCGG AGGCTCCGGA GGGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG ATGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGAAT CGATAGCGAT AAGGATCCTC TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC TGACATTCAT CCGGGGTCAG CACCGTTTCT GCGGACTGGC TTTCTACGTA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTCAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACTATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAATT CGAAATGACC GACCAAGCGA CGCCCAACCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATT TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTATAGTC CGGAAATACA GGAACGCACG CTGGATGGCC CTTCGCTGGG ATGGTGAAAC CATGAAAAAT GGCAGCTTCA GTGGATTAAG TGGGGGTAAT GTGGCCTGTA CCCTCTGGTT GCATAGGTAT TCATACGGTT AAAATTTATC AGGCGCGATT GCGGCAGTTT TTCGGGTGGT TTGTTGCCAT TTTTACCTGT CTGCTGCCGT GATCGCGCTG AACGCGTTTT AGCGGTGCGT ACAATTAAGG GATTATGGTA AATCCACTTA CTGTCTGCCC TCGTAGCCAT CGAGATAAAC CGCAGTACTC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
