pAAV-cTNT-CasRx-U6-gRNA1-gRNA2
$99.00
In stock
SKU
PL63319
100μg liquid plasmid(Instantly):$120
| name | pAAV-cTNT-CasRx-U6-gRNA1-gRNA2 | another name | apAAV-cTNT-CasRx-U6-gRNA1-gRNA2 |
| Insert Size (bp) | Plasmid host | Mammary cell,Adeno-associated virus | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | cTNT,U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT AGGGGTTCCT GCGGCCGCAC GCGTAAGCTT CGGGCCCCCC CTCGAGGTCG GGATAAAAGC AGTCTGGGCT TTCACATGAC AGCATCTGGG GCTGCGGCAG AGGGTCGGGT CCGAAGCGCT GCCTTATCAG CGTCCCCAGC CCTGGGAGGT GACAGCTGGC TGGCTTGTGT CAGCCCCTCG GGCACTCACG TATCTCCGTC CGACGGGTTT AAAATAGCAA AACTCTGAGG CCACACAATA GCTTGGGCTT ATATGGGCTC CTGTGGGGGA AGGGGGAGCA CGGAGGGGGC CGGGGCCGCT GCTGCCAAAA TAGCAGCTCA CAAGTGTTGC ATTCCTCTCT GGGCGCCGGG CACATTCCTG CTGGCTCTGC CCGCCCCGGG GTGGGCGCCG GGGGGACCTT AAAGCCTCTG CCCCCCAAGG AGCCCTTCCC AGACAGCCGC CGGCACCCAC CGCTCCGTGG GACGATCCCC GAAGCTCTAG AGCTTTATTG CGGTAGTTTA TCACAGTTAA ATTGCTAACG CAGTCAGTGC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA GGGTACGACC GGTGCCACCA TGCCTAAAAA GAAAAGAAAG GTGGGTTCTG GTATCGAAAA AAAAAAGTCC TTCGCCAAGG GCATGGGCGT GAAGTCCACA CTCGTGTCCG GCTCCAAAGT GTACATGACA ACCTTCGCCG AAGGCAGCGA CGCCAGGCTG GAAAAGATCG TGGAGGGCGA CAGCATCAGG AGCGTGAATG AGGGCGAGGC CTTCAGCGCT GAAATGGCCG ATAAAAACGC CGGCTATAAG ATCGGCAACG CCAAATTCAG CCATCCTAAG GGCTACGCCG TGGTGGCTAA CAACCCTCTG TATACAGGAC CCGTCCAGCA GGATATGCTC GGCCTGAAGG AAACTCTGGA AAAGAGGTAC TTCGGCGAGA GCGCTGATGG CAATGACAAT ATTTGTATCC AGGTGATCCA TAACATCCTG GACATTGAAA AAATCCTCGC CGAATACATT ACCAACGCCG CCTACGCCGT CAACAATATC TCCGGCCTGG ATAAGGACAT TATTGGATTC GGCAAGTTCT CCACAGTGTA TACCTACGAC GAATTCAAAG ACCCCGAGCA CCATAGGGCC GCTTTCAACA ATAACGATAA GCTCATCAAC GCCATCAAGG CCCAGTATGA CGAGTTCGAC AACTTCCTCG ATAACCCCAG ACTCGGCTAT TTCGGCCAGG CCTTTTTCAG CAAGGAGGGC AGAAATTACA TCATCAATTA CGGCAACGAA TGCTATGACA TTCTGGCCCT CCTGAGCGGA CTGAGGCACT GGGTGGTCCA TAACAACGAA GAAGAGTCCA GGATCTCCAG GACCTGGCTC TACAACCTCG ATAAGAACCT CGACAACGAA TACATCTCCA CCCTCAACTA CCTCTACGAC AGGATCACCA ATGAGCTGAC CAACTCCTTC TCCAAGAACT CCGCCGCCAA CGTGAACTAT ATTGCCGAAA CTCTGGGAAT CAACCCTGCC GAATTCGCCG AACAATATTT CAGATTCAGC ATTATGAAAG AGCAGAAAAA CCTCGGATTC AATATCACCA AGCTCAGGGA AGTGATGCTG GACAGGAAGG ATATGTCCGA GATCAGGAAA AATCATAAGG TGTTCGACTC CATCAGGACC AAGGTCTACA CCATGATGGA CTTTGTGATT TATAGGTATT ACATCGAAGA GGATGCCAAG GTGGCTGCCG CCAATAAGTC CCTCCCCGAT AATGAGAAGT CCCTGAGCGA GAAGGATATC TTTGTGATTA ACCTGAGGGG CTCCTTCAAC GACGACCAGA AGGATGCCCT CTACTACGAT GAAGCTAATA GAATTTGGAG AAAGCTCGAA AATATCATGC ACAACATCAA GGAATTTAGG GGAAACAAGA CAAGAGAGTA TAAGAAGAAG GACGCCCCTA GACTGCCCAG AATCCTGCCC GCTGGCCGTG ATGTTTCCGC CTTCAGCAAA CTCATGTATG CCCTGACCAT GTTCCTGGAT GGCAAGGAGA TCAACGACCT CCTGACCACC CTGATTAATA AATTCGATAA CATCCAGAGC TTCCTGAAGG TGATGCCTCT CATCGGAGTC AACGCTAAGT TCGTGGAGGA ATACGCCTTT TTCAAAGACT CCGCCAAGAT CGCCGATGAG CTGAGGCTGA TCAAGTCCTT CGCTAGAATG GGAGAACCTA TTGCCGATGC CAGGAGGGCC ATGTATATCG ACGCCATCCG TATTTTAGGA ACCAACCTGT CCTATGATGA GCTCAAGGCC CTCGCCGACA CCTTTTCCCT GGACGAGAAC GGAAACAAGC TCAAGAAAGG CAAGCACGGC ATGAGAAATT TCATTATTAA TAACGTGATC AGCAATAAAA GGTTCCACTA CCTGATCAGA TACGGTGATC CTGCCCACCT CCATGAGATC GCCAAAAACG AGGCCGTGGT GAAGTTCGTG CTCGGCAGGA TCGCTGACAT CCAGAAAAAA CAGGGCCAGA ACGGCAAGAA CCAGATCGAC AGGTACTACG AAACTTGTAT CGGAAAGGAT AAGGGCAAGA GCGTGAGCGA AAAGGTGGAC GCTCTCACAA AGATCATCAC CGGAATGAAC TACGACCAAT TCGACAAGAA AAGGAGCGTC ATTGAGGACA CCGGCAGGGA AAACGCCGAG AGGGAGAAGT TTAAAAAGAT CATCAGCCTG TACCTCACCG TGATCTACCA CATCCTCAAG AATATTGTCA ATATCAACGC CAGGTACGTC ATCGGATTCC ATTGCGTCGA GCGTGATGCT CAACTGTACA AGGAGAAAGG CTACGACATC AATCTCAAGA AACTGGAAGA GAAGGGATTC AGCTCCGTCA CCAAGCTCTG CGCTGGCATT GATGAAACTG CCCCCGATAA GAGAAAGGAC GTGGAAAAGG AGATGGCTGA AAGAGCCAAG GAGAGCATTG ACAGCCTCGA GAGCGCCAAC CCCAAGCTGT ATGCCAATTA CATCAAATAC AGCGACGAGA AGAAAGCCGA GGAGTTCACC AGGCAGATTA ACAGGGAGAA GGCCAAAACC GCCCTGAACG CCTACCTGAG GAACACCAAG TGGAATGTGA TCATCAGGGA GGACCTCCTG AGAATTGACA ACAAGACATG TACCCTGTTC AGAAACAAGG CCGTCCACCT GGAAGTGGCC AGGTATGTCC ACGCCTATAT CAACGACATT GCCGAGGTCA ATTCCTACTT CCAACTGTAC CATTACATCA TGCAGAGAAT TATCATGAAT GAGAGGTACG AGAAAAGCAG CGGAAAGGTG TCCGAGTACT TCGACGCTGT GAATGACGAG AAGAAGTACA ACGATAGGCT CCTGAAACTG CTGTGTGTGC CTTTCGGCTA CTGTATCCCC AGGTTTAAGA ACCTGAGCAT CGAGGCCCTG TTCGATAGGA ACGAGGCCGC CAAGTTCGAC AAGGAGAAAA AGAAGGTGTC CGGCAATTCC GGTTCTGGTT ACCCATACGA CGTACCAGAT TACGCTCCCA AGAAGAAGCG CAAGGTGGGA TAAAGATCCT AAAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGATCGCG GCCGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA GGCTGTTAGA GAGATAATTG GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAACA CCGAACCCCT ACCAACTGGT CGGGGTTTGA AACGGGGAAG CTACACTGTT GTCCAAGCCA TCACAAGTAA ACCCCTACCA ACTGGTCGGG GTTTGAAACA CAGGAGCTGG AGATGCCCCC TGAGGCACCC AAGTAAACCC CTACCAACTG GTCGGGGTTT GAAACTTTTT TTGAATTCTG ATGCGGTGGC GGCCGCCACG TGCGGACCGA GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCT GCCTGCAGGG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA AAGCAACCAT AGTACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC TTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACTCTAT CTCGGGCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGTCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGT
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