pUC57-MET(human)-D1228N
$171.00
In stock
SKU
PL61564
100μg liquid plasmid(Instantly):$192
| name | pUC57-MET(human)-D1228N | another name | apUC57-MET(human)-D1228N |
| Insert Size (bp) | 4191bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13R： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTCGAGGCCA CCATGAAGGC CCCCGCTGTG CTTGCACCTG GCATCCTCGT GCTCCTGTTT ACCTTGGTGC AGAGGAGCAA TGGGGAGTGT AAAGAGGCAC TAGCAAAGTC CGAGATGAAT GTGAATATGA AGTATCAGCT TCCCAACTTC ACCGCGGAAA CACCCATCCA GAATGTCATT CTACATGAGC ATCACATTTT CCTTGGTGCC ACTAACTACA TTTATGTTTT AAATGAGGAA GACCTTCAGA AGGTTGCTGA GTACAAGACT GGGCCTGTGC TGGAACACCC AGATTGTTTC CCATGTCAGG ACTGCAGCAG CAAAGCCAAT TTATCAGGAG GTGTTTGGAA AGATAACATC AACATGGCTC TAGTTGTCGA CACCTACTAT GATGATCAAC TCATTAGCTG TGGCAGCGTC AACAGAGGGA CCTGCCAGCG ACATGTCTTT CCCCACAATC ATACTGCTGA CATACAGTCG GAGGTTCACT GCATATTCTC CCCACAGATA GAAGAGCCCA GCCAGTGTCC TGACTGTGTG GTGAGCGCCC TGGGAGCCAA AGTCCTTTCA TCTGTAAAGG ACCGGTTCAT CAACTTCTTT GTAGGCAATA CCATAAATTC TTCTTATTTC CCAGATCATC CATTGCATTC GATATCAGTG AGAAGGCTAA AGGAAACGAA AGATGGTTTT ATGTTTTTGA CGGACCAGTC CTACATTGAT GTTTTACCTG AGTTCAGAGA TTCTTACCCC ATTAAGTATG TCCATGCCTT TGAAAGCAAC AATTTTATTT ACTTCTTGAC GGTCCAAAGG GAAACTCTAG ATGCTCAGAC TTTTCACACA AGAATAATCA GGTTCTGTTC CATAAACTCT GGATTGCATT CCTACATGGA AATGCCTCTG GAGTGTATTC TCACAGAAAA GAGAAAAAAG AGATCCACAA AGAAGGAAGT GTTTAATATA CTTCAGGCTG CGTATGTCAG CAAGCCTGGG GCCCAGCTTG CTAGACAAAT AGGAGCCAGC CTGAATGATG ACATTCTTTT CGGGGTGTTC GCACAAAGCA AGCCAGATTC TGCCGAACCA ATGGATCGAT CTGCCATGTG TGCATTCCCT ATCAAATATG TCAACGACTT CTTCAACAAG ATCGTCAACA AAAACAATGT GAGATGTCTC CAGCATTTTT ACGGACCCAA TCATGAGCAC TGCTTTAATA GGACACTTCT GAGAAATTCA TCAGGCTGTG AAGCGCGCCG TGATGAATAT CGAACAGAGT TTACCACAGC TTTGCAGCGC GTTGACTTAT TCATGGGTCA ATTCAGCGAA GTCCTCTTAA CATCTATATC CACCTTCATT AAAGGAGACC TCACCATAGC TAATCTTGGG ACATCAGAGG GTCGCTTCAT GCAGGTTGTG GTTTCTCGAT CAGGACCATC AACCCCTCAT GTGAATTTTC TCCTGGACTC CCATCCAGTG TCTCCAGAAG TGATTGTGGA GCATACATTA AACCAAAATG GCTACACACT GGTTATCACT GGGAAGAAGA TCACGAAGAT CCCATTGAAT GGCTTGGGCT GCAGACATTT CCAGTCCTGC AGTCAATGCC TCTCTGCCCC ACCCTTTGTT CAGTGTGGCT GGTGCCACGA CAAATGTGTG CGATCGGAGG AATGCCTGAG CGGGACATGG ACTCAACAGA TCTGTCTGCC TGCAATCTAC AAGGTTTTCC CAAATAGTGC ACCCCTTGAA GGAGGGACAA GGCTGACCAT ATGTGGCTGG GACTTTGGAT TTCGGAGGAA TAATAAATTT GATTTAAAGA AAACTAGAGT TCTCCTTGGA AATGAGAGCT GCACCTTGAC TTTAAGTGAG AGCACGATGA ATACATTGAA ATGCACAGTT GGTCCTGCCA TGAATAAGCA TTTCAATATG TCCATAATTA TTTCAAATGG CCACGGGACA ACACAATACA GTACATTCTC CTATGTGGAT CCTGTAATAA CAAGTATTTC GCCGAAATAC GGTCCTATGG CTGGTGGCAC TTTACTTACT TTAACTGGAA ATTACCTAAA CAGTGGGAAT TCTAGACACA TTTCAATTGG TGGAAAAACA TGTACTTTAA AAAGTGTGTC AAACAGTATT CTTGAATGTT ATACCCCAGC CCAAACCATT TCAACTGAGT TTGCTGTTAA ATTGAAAATT GACTTAGCCA ACCGAGAGAC AAGCATCTTC AGTTACCGTG AAGATCCCAT TGTCTATGAA ATTCATCCAA CCAAATCTTT TATTAGTGGT GGGAGCACAA TAACAGGTGT TGGGAAAAAC CTGAATTCAG TTAGTGTCCC GAGAATGGTC ATAAATGTGC ATGAAGCAGG AAGGAACTTT ACAGTGGCAT GTCAACATCG CTCTAATTCA GAGATAATCT GTTGTACCAC TCCTTCCCTG CAACAGCTGA ATCTGCAACT CCCCCTGAAA ACCAAAGCCT TTTTCATGTT AGATGGGATC CTTTCCAAAT ACTTTGATCT CATTTATGTA CATAATCCTG TGTTTAAGCC TTTTGAAAAG CCAGTGATGA TCTCAATGGG CAATGAAAAT GTACTGGAAA TTAAGGGAAA TGATATTGAC CCTGAAGCAG TTAAAGGTGA AGTGTTAAAA GTTGGAAATA AGAGCTGTGA GAATATACAC TTACATTCTG AAGCCGTTTT ATGCACGGTC CCCAATGACC TGCTGAAATT GAACAGCGAG CTAAATATAG AGTGGAAGCA AGCAATTTCT TCAACCGTCC TTGGAAAAGT AATAGTTCAA CCAGATCAGA ATTTCACAGG ATTGATTGCT GGTGTTGTCT CAATATCAAC AGCACTGTTA TTACTACTTG GGTTTTTCCT GTGGCTGAAA AAGAGAAAGC AAATTAAAGA TCTGGGCAGT GAATTAGTTC GCTACGATGC AAGAGTACAC ACTCCTCATT TGGATAGGCT TGTAAGTGCC CGAAGTGTAA GCCCAACTAC AGAAATGGTT TCAAATGAAT CTGTAGACTA CCGAGCTACT TTTCCAGAAG ATCAGTTTCC TAATTCATCT CAGAACGGTT CATGCCGACA AGTGCAGTAT CCTCTGACAG ACATGTCCCC CATCCTAACT AGTGGGGACT CTGATATATC CAGTCCATTA CTGCAAAATA CTGTCCACAT TGACCTCAGT GCTCTAAATC CAGAGCTGGT CCAGGCAGTG CAGCATGTAG TGATTGGGCC CAGTAGCCTG ATTGTGCATT TCAATGAAGT CATAGGAAGA GGGCATTTTG GTTGTGTATA TCATGGGACT TTGTTGGACA ATGATGGCAA GAAAATTCAC TGTGCTGTGA AATCCTTGAA CAGAATCACT GACATAGGAG AAGTTTCCCA ATTTCTGACC GAGGGAATCA TCATGAAAGA TTTTAGTCAT CCCAATGTCC TCTCGCTCCT GGGAATCTGC CTGCGAAGTG AAGGGTCTCC GCTGGTGGTC CTACCATACA TGAAACATGG AGATCTTCGA AATTTCATTC GAAATGAGAC TCATAATCCA ACTGTAAAAG ATCTTATTGG CTTTGGTCTT CAAGTAGCCA AAGGCATGAA ATATCTTGCA AGCAAAAAGT TTGTCCACAG AGACTTGGCT GCAAGAAACT GTATGCTGGA TGAAAAATTC ACAGTCAAGG TTGCTGATTT TGGTCTTGCC AGAAACATGT ATGATAAAGA ATACTATAGT GTACACAACA AAACAGGTGC AAAGCTGCCA GTGAAGTGGA TGGCTTTGGA AAGTCTGCAA ACTCAAAAGT TTACCACCAA GTCAGATGTG TGGTCCTTTG GCGTGCTCCT CTGGGAGCTG ATGACAAGAG GAGCCCCACC TTATCCTGAC GTAAACACCT TTGATATAAC TGTTTACTTG TTGCAAGGGA GAAGACTCCT ACAACCCGAA TACTGCCCAG ACCCCTTATA TGAAGTAATG CTAAAATGCT GGCACCCTAA AGCCGAAATG CGCCCATCCT TTTCTGAACT GGTGTCCCGG ATATCAGCGA TCTTCTCTAC TTTCATTGGG GAGCACTATG TCCATGTGAA CGCTACTTAT GTGAACGTAA AATGTGTCGC TCCGTATCCT TCTCTGTTGT CATCAGAAGA TAACGCTGAT GATGAGGTGG ACACACGACC AGCCTCCTTC TGGGAGACAT CATGGCGCGC C
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