pEX18Tet-phes
$128.00
In stock
SKU
PL61357
100μg liquid plasmid(Instantly):$149
| name | pEX18Tet-phes | another name | apEX18Tet-phes |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Tet | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATAATCG GCATTTTCTT TTGCGTTTTT ATTTGTTAAC TGTTAATTGT CCTTGTTCAA GGATGCTGTC TTTGACAACA GATGTTTTCT TGCCTTTGAT GTTCAGCAGG AAGCTAGGCG CAAACGTTGG ATCTTTAGGC GAACTGGCGG AGGAACCGGA GGTCATTTTC GAAAAAGAGC CGGAGGTCCT GGACACCATA CCGGAGCATG GTAAGGCGTT CCAACCCGGA ACCGAAGCCG AAGCCAATGT AACGCTCCGG GTCGAGGCCC ATATTCCGAA TGACCGTGGG GTGGACCTGA CCGCTGCCGC TGATTTCCAA CCACTTACCA GCATTCTTCC CATGTTCAAA CATCATATCA ATTTCCGCGC TGGGTTCCGT GAAGGGGAAG TAGCTCGGGC GGAAACGGAC CAAGATGTCA TCTCGTTCGA AAAATTTCTT GAGAAAGTCG GTGTAGGCAC CCTTGAGATC GGCAAACGAC ACGTTTTCAT CAATCCAGAG CCCCTCCACC TGATTAAACA TGGGGCTATG GGTGGCGTCC GAATCGACCC GATAGGTCCG ACCGGGAGCA ATCACCTTAA TCGGGGGTCG ATTCATGCGG GCGTACCGCA CCTGCATGGG CGAGGTATGG GTCCGGAGGA GGAGGGGCCG CCCATCGGCG TCCTTACCAT CCACATAGAA GGTGTCCTGC ATGGACCGTG CGGGGTGGTT TTCGGGCGAG TTGAGCGCCG TGAAATTATA CCAATCCGTC TCAATTTCCG GTCCATCCGC GACATCGAAG CCAATGCTAC GGAAAATCTG CTCCACACGT TCCCAGGTTC GCATGACGGG GTGCAGCGAG CCGGCGCCCG CCCCGCGACC GGGGAGGGTC ACATCAATGG CTTCAGCCGC CAGGCGCTGA TTGAGAAGGG CATCCGCCAA GGCCTGCCGC CGGGCATTCA AGGCCGCCTC CACCTGCTGC TTGGCCACGT TAATCCGGGC ACCCTCGGTC TTCCGGGTTT CCGGGTCCAG CTTACCCAGC CCCTTCAAGA GTTCCGTGAG GGCACCGCTC TTGCCCAGGA AGCGGGCCTT TTCATTTTCC AAGGTCGTAA TGTCGGCCGC GCCCTCAAAG CTCTGCTGGG CATCCGCCAC GATCTGATCG AGGTCCATAT GCTTGTCCTT TCGAAGCTCG ATCCGATTGT CAGACCCGGC ATGATATTCC GAAAATCCCA AGCGAGTCAA CAGCTAGTGC TTGCCCTCAG TACTGTTACG CCGGCGGTAG CCGGCCAGCC TCGCAGAGCA GGATTCCCGT TGAGCACCGC CAGGTGCGAA TAAGGGACAG TGAAGAAGGA ACACCCGCTC GCGGGTGGGC CTACTTCACC TATCCTGCCC GGCTGACGCC GTTGGATACA CCAAGGAAAG TCTACACGAA CCCTTTGGCA AAATCCTGTA TATCGTGCGA AAAAGGATGG ATATACCGAA AAAATCGCTA TAATGACCCC GAAGCAGGGT TATGCAGCGG AAAAGCGCTG CTTCCCTGCT GTTTTGTGGA ATATCTACCG ACTGGAAACA GGCAAATGCA GGAAATTACT GAACTGAGGG GACAGGCGAG AGACGATGCC AAAGAGCTAC ACCGACGAGC TGGCCGAGTG GGTTGAATCC CGCGCGGCCA AGAAGCGCCG GCGTGATGAG GCTGCGGTTG CGTTCCTGGC GGTGAGGGCG GATGTCGATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCATATTTG AATGTATTTA GAAAAATAAA CAAAAGAGTT TGTAGAAACG CAAAAAGGCC ATCCGTCAGG ATGGCCTTCT GCTTAATTTG ATGCCTGGCA GTTTATGGCG GGCGTCCTGC CCGCCACCCT CCGGGCCGTT GCTTCGCAAC GTTCAAATCC GCTCCCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA AAGGCCCAGT CTTTCGACTG AGCCTTTCGT TTTATTTGAT GCCTGGCAGT TCCCTACTCT CGCATGGGGA GACCCCACAC TACCATCGGC GCTACGGCGT TTCACTTCTG AGTTCGGCAT GGGGTCAGGT GGGACCACCG CGCTACTGCC GCCAGGCAAA TTCTGTTTTA TCAGACCGCT TCTGCGTTCT GATTTAATCT GTATCAGGCT GAAAATCTTC TCTCATCCGC CAAAACAGCC AAGCTCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA CCGAGCTCGA ATTCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAATCGATG CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GGCGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GTAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGAATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTAATCGG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TC
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