NM_001080118.1;AI480703; CRSP; CRSP210; DRIP; DRIP205;l11Jus; l11Jus15; P; PBP; Pparbp; TRAP; TRAP220; TRIP-2
100μg liquid plasmid(Instantly):$163
| name | pCMV-FLAG-MED1(mouse) | another name | apCMV-FLAG-MED1(mouse) |
| Insert Size (bp) | 4278bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTGATCT ATACATTGAA TCAATATTGG CAATTAGCCA TATTAGTCAT TGGTTATATA GCATAAATCA ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA ATTGATCTAC CATGGACTAC AAAGACGATG ACGACAAGCT TATGAAGGCT CAGGGGGAAA CCGAGGACTC AGAGAGGCTG AGTAAGATGA GCTCCCTCCT GGAACGGCTC CATGCAAAAT TTAACCAGAA CAGACCTTGG AGTGAAACCA TTAAGCTTGT GCGTCAAGTA ATGGAGAAGA GGGTCGTAAT GAGTTCTGGA GGGCATCAGC ATTTGGTCAG CTGTTTGGAG ACATTGCAGA AGGCTCTCAA AGTAACATCT TTGCCAGCAA TGACTGATCG TTTGGAATCT ATAGCCAGAC AGAATGGACT GGGCTCTCAC CTCAGTGCCA GTGGCACTGA GTGTTACATC ACGTCAGATA TGTTCTATGT GGAAGTGCAG TTAGATCCTG CAGGACAGCT TTGTGATGTC AAAGTGGCTC ACCATGGGGA GAATCCTGTG AGCTGTCCAG AGCTTGTACA GCAGTTAAGG GAAAAGAATT TTGAGGAATT TTCCAAGCAT CTTAAGGGTC TTGTTAATCT GTATAATCTC CCAGGGGACA ACAAACTGAA GACTAAAATG TATCTGGCTC TCCAATCCTT AGAACAGGAC CTTTCTAAAA TGGCTATTAT GTACTGGAAG GCAACCAACG CCGCTCCCTT GGATAAGATT CTTCATGGAA GTGTTGGTTA TCTCACCCCG CGGAGTGGGG GTCATTTAAT GAATATGAAA TACTATGCCT CTCCATCTGA CCTGCTGGAT GATAAGACTG CCTCTCCTAT CATTTTGCAT GAAAAGAATG TTCCTCGGTC TTTGGGAATG AATGCCTCAG TGACAATTGA AGGAACCTCT GCTATGTACA AACTCCCAAT TGCCCCATTA ATTATGGGGT CACACCCAGC TGACAACAAA TGGACCCCTT CTTTCTCCGC AGTCACTAGT GCCAACAGTG TTGATCTTCC TGCGTGTTTC TTCTTGAAAT TTCCCCAGCC AATTCCAGTA TCTAAAGCAT TTGTTCAGAA ACTGCAAAAT TGCACAGGAA TCCCGTTGTT TGAGACTCCG CCCACTTACC TGCCCCTGTA TGAACTCATC ACTCAGTTTG AGCTGTCAAA GGATCCTGAC CCTTTACCTT TGAATCACAA CATGCGATTT TACGCTGCTC TTCCAGGTCA GCAGCACTGC TATTTTCTCA ATAAAGATGC TCCTCTTCCT GATGGTCAGA GCCTGCAGGG AACACTGGTC AGCAAAATCA CCTTCCAGCA CCCTGGCCGA GTTCCTCTTA TCTTGAATAT GATCAGACAC CAAGTGGCCT ATAACACTCT AATTGGAAGC TGTGTCAAAA GAACTATTTT AAAAGAAGAT TCTCCTGGGC TCCTCCAGTT TGAAGTGTGT CCTCTCTCAG AATCTCGCTT CAGTGTATCT TTTCAGCACC CTGTGAATGA CTCCCTTGTG TGTGTGGTGA TGGATGTGCA AGACTCAACA CATGTGAGCT GTAAACTCTA CAAGGGGCTG TCAGATGCAC TAATCTGTAC AGACGACTTC ATTGCCAAAG TTGTTCAAAG ATGTATGTCC ATTCCTGTGA CGATGAGGGC TATTCGGAGG AAGGCTGAAA CCATACAGGC TGACACCCCA GCACTGTCTC TCATTGCAGA GACAGTTGAA GACATGGTGA AAAAGAACCT GCCCCCGGCT AGCAGCCCAG GGTATGGCAT GACCACAGGC AACAACCCAA TGAGTGGTAC CACTACACCA ACCAACACCT TTCCGGGGGG TCCCATTACC ACCTTGTTTA ATATGAGCAT GAGCATCAAA GATCGGCATG AGTCGGTGGG CCATGGGGAG GACTTCAGCA AGGTGTCTCA GAACCCAATT CTTACCAGTT TGTTGCAAAT CACAGGGAAC GGGGGGTCTA CCATTGGCTC GAGTCCGACC CCTCCTCATC ACACGCCGCC ACCTGTCTCT TCGATGGCCG GCAACACCAA GAACCACCCG ATGCTCATGA ACCTTCTTAA AGATAACCCT GCCCAGGATT TCTCAACCCT TTATGGAAGC AGCCCTTTAG AAAGGCAGAA CTCCTCTTCC GGATCACCCC GGATGGAAAT GTGCTCGGGG AGCAACAAGG CCAAGAAGAA GAAGTCGTCA AGAGTCCCAC CTGACAAACC CAAGCACCAG ACTGAAGACG ATTTCCAGAG GGAGCTCTTT TCCATGGATG TCGACTCACA GAACCCTATG TTTGACGTCA GCATGACCGC TGACGCGCTG GATACACCTC ATATCACCCC AGCTCCAAGC CAGTGTAGCA CTCCCCCAGC AACGTACCCA CAGCCAGTGT CTCACCCCCA GCCCAGTATT CAGAGGATGG TCCGACTGTC CAGTTCAGAC AGCATTGGCC CAGATGTAAC TGATATTCTT TCAGATATTG CCGAAGAAGC TTCAAAGCTT CCCAGCACGA GTGATGACTG CCCACCAATT GGCACCCCTG TTCGAGATTC CTCAAGTTCT GGGCATTCTC AGAGTGCCCT CTTTGATTCT GATGTCTTTC AAACTAATAA TAATGAAAAT CCATACACTG ATCCAGCTGA CCTTATTGCA GATGCTGCTG GAAGCCCCAA TAGTGATTCT CCTACCAATC ATTTTTTCCC TGATGGAGTA GATTTCAATC CTGATTTGTT GAACAGCCAA AGCCAAAGTG GTTTTGGAGA GGAGTATTTT GATGAAAGTA GTCAGAGTGG GGATAATGAT GATTTCAAAG GATTTGCATC TCAGGCATTA AATACATTGG GGATGCCAAT GCTTGGAGGT GACAATGGGG AGCCAAAATT TAAGGGCAGC AGCCAGGCTG ACACGGTGGA CTTCAGTATT ATATCAGTAG CCGGTAAGGC TTTGGGTGCT GCAGATCTGA TGGAGCACCA CAGTGGGAGT CAGAGTCCTT TACTGACCAC TGGAGAATTA GGGAAAGAAA AAACTCAAAA GAGGGTGAAG GAAGGCAACG GCACAGGTGC TAGCAGTGGA TCAGGTCCAG GGTCAGACAG CAAGCCAGGC AAGCGCAGCC GCACTCCCTC CAATGATGGG AAGAGCAAGG ATAAGCCTCC AAAGCGGAAG AAGGCAGACA CTGAGGGGAA GTCCCCATCT CACAGTTCTT CTAATAGACC TTTCACCCCA CCTACCAGCA CGGGTGGGTC CAAATCCCCA GGCAGTTCAG GACGATCTCA GACGCCCCCA GGTGTTGCCA CCCCGCCCAT TCCCAAGATT ACCATTCAGA TTCCTAAAGG GACAGTGATG GTGGGCAAGC CCTCCTCTCA CAGTCAGTAC ACTAGCAGTG GTTCTGTGTC TTCCTCTGGC AGCAAAAGCC ACCATAGTCA TTCTTCCTCC TCCTCTTCCT TAGCTTCTGC TTCCACCTCA GGCAAGGTGA AAAGCAGTAA ATCTGAAGGC TCATCAAGTT CCAAGCTCAG TGGCAGTATG TATGCTAGCC AAGGGTCTTC TGGATCCAGC CAGTCCAAAA ATTCATCTCA GACTGGGGGG AAGCCAGGCT CCTCTCCCAT TACCAAACAT GGACTGAGCA GTGGGTCCAG CAGTACCAAG ATGAAACCTC AAGGCAAGCC ATCCTCCCTT ATGAACCCTT CTATAAGTAA GCCAAACATA TCCCCTTCCC ATTCAAGGCC TCCCGGAGGC TCAGATAAGC TTGCCTCTCC AATGAAGCCT GTTCCTGGAA CCCCCCCATC CTCTAAAGCC AAGTCCCCTA TCAGTTCAGG TTCCAGTGGT TCTCATGTGT CAGGAACTAG TTCAAGCTCT GGTATGAAGT CATCTTCAGG GTCAGCATCC TCAGGCTCAG TGTCTCAAAA AACCCCTCCA GCATCTAATT CTTGTACACC ATCTTCCTCT TCGTTTTCCT CAAGTGGTTC TTCCATGTCA TCCTCTCAGA ATCAACATGG CAGTTCCAAA GGGAAATCTC CCAGTAGGAA TAAGAAGCCT TCCTTGACAG CTGTCATAGA TAAATTGAAG CATGGGGTGG TTACCAGTGG GCCTGGGGGT GAGGATCCAA TAGACAGTCA GATGGGCGCA AGCACAAATT CTTCTAACCA TCCCATGTCC TCCAAACATA ACACGTCAGG AGGGGAGTTC CAGAGCAAAC GTGAGAAAAG TGATAAAGAC AAATCCAAGG TCTCTGCTTC TGGGGGGTCA GTGGATTCCT CTAAGAAGAC TTCAGAGTCA AAAAATGTGG GGAGCACGGG GGTGGCAAAA ATCATTATCA GCAAGCACGA CGGAGGCTCC CCGAGCATCA AAGCCAAAGT GACGCTACAG AAACCTGGAG AAAGTGGTGG AGATGGGCTC AGGCCACAGA TAGCCTCATC AAAGAACTAT GGCTCTCCAC TTATCAGTGG TTCCACTCCA AAGCACGAAC GGGGTTCTCC CAGCCACAGT AAGTCGCCAG CATATACACC ACAGAATGTG GACAGTGAAA GTGAGTCAGG CTCCTCCATA GCAGAGAGAT CCTACCAGAA CAGTCCCAGC TCAGAGGATG GTATCCGACC ACTTCCAGAG TACAGCACTG AGAAGCATAA GAAGCACAAA AAGGAAAAGA AGAAAGTCAG AGACAAAGAC AGAGACAAGA AGAAGTCTCA CAGCATGAAG CCAGAGAACT GGTCGAAATC CCCCATTTCT TCAGATCCGA CGGCGTCTGT GACAAATAAC CCTATCTTAT CTGCAGACAG GCCTTCTAGG CTCAGCCCTG ACTTCATGAT TGGGGAGGAA GATGATGATC TCATGGATGT GGCCCTGATT GGCAATTAGG GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCTCGAGG AACTGAAAAA CCAGAAAGTT AATTCCCTAT AGTGAGTCGT ATTAAATTCG TAATCATGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTT
