pJB658
$128.00
In stock
SKU
PL61310
100μg liquid plasmid(Instantly):$163
| name | pJB658 | another name | apJB658 |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | pm | Replicon | oriV |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAGATCCCCT TTATCCGCCA ATTCGTCGCC GCATGCCCCG ACAGCACGAA CTTCTGGTGT TCTCGCTTCT TAAAAAGAAC GTCTTCGTTC TGCTTGGCGT TATTTTTGCT TGGAAAAGTG GTCACTGATT GCAAAAAGGA TGGCGCAACG TGGCAATGGG GGTAACCCGT ATACGCATCA CGTCGAGATG CATTTTCATC GACTTGGCGC CTTTCTACAT CACACCAAGC AGCCCACATT AAAATAAGAG AACCGTGAAC TATGGATTTT TGCTTATTGA ACGAGAAAAG TCAGATCTTC GTCCACGCCG AGCCCTATGC AGTCTCCGAT TATGTTAACC AGTATGTCGG TACGCACTCT ATTCGCCTGC CCAAGGGCGG GCCCCCGGCA GGCAGGCTGC ACCACAGAAT CTTCGGATGC CTCGACCTGT GTCGAATCAG CTACGGCGGT AGCGTGAGGG TAATCTCGCC TGGATTAGAG ACCTGTTATC ATCTGCAAAT AATACTCAAA GGCCATTGCC TGTGGCGTGG CCATGGCCAG GAGCACTATT TTGCGCCGGG CGAACTATTG CTGCTCAATC CGGATGACCA AGCCGACCTG ACCTATTCAG AAGATTGCGA GAAATTTATC GTTAAATTGC CCTCAGTGGT CCTTGATCGG GCATGCAGTG ACAACAATTG GCACAAGCCG AGGGAGGGTA TCCGTTTCGC CGCGCGACAC AATCTCCAGC AACTCGATGG CTTTATCAAT CTACTCGGGT TAGTTTGTGA CGAAGCGGAA CATACAAAGT CGATGCCTCG GGTCCAAGAG CACTATGCGG GGATCATCGC TTCCAAGCTG CTCGAAATGC TGGGCAGCAA TGTCAGCCGT GAAATTTTCA GCAAAGGTAA CCCGTCTTTC GAGCGAGTCG TTCAATTCAT TGAGGAGAAT CTCAAACGGA ATATCAGCCT TGAGCGGTTA GCGGAGCTGG CGATGATGAG TCCACGCTCG CTCTACAATT TGTTCGAGAA GCATGCCGGC ACCACGCCGA AGAACTACAT CCGCAACCGC AAGCTCGAAA GCATCCGCGC CTGCTTGAAC GATCCCAGTG CCAATGTGCG TAGTATAACT GAGATAGCCC TAGACTACGG CTTCTTACAT TTGGGACGCT TCGCTGAAAA CTATAGGAGC GCGTTCGGCG AGTTGCCTTC CGACACCCTG CGTCAATGCA AAAAGGAAGT GGCTTGATTA CGAACGTAGC CGAAGAAGGG ATGGGTTGGC ATCGCCCCGG CTTTCTTAGA CACTCTCCAA GCTCTGAAAT AGCGTTTTAC AAACTCCACT GGCTATAGTG CCGGCGTTTG CCCGCGCTCT CCGTGGCCGC GTCCCAATGC AGGTCGGGTT CCTCGACCCG AAGTGCCCTT GGGCATCTCC ACTTCAGCGT CTGACCTTGC TGGCCAGTTC GCCGCCGATG CCGAAGTGGC CCTTGGCCAT CTCCGTGATA ATCACTCGCA CGCTGGTCAG CGGCGCATCC AGGGAGCGCG AGATGGCCTC GCTGACTTCC CGAATGAGGG TTTCCTTCTG CTCGTCGCTG CGGCCTTCAA GGATGTGGAT CTGCTGGCCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GAGCTTACGG CCAGCCTCGC AGAGCAGGAT TCCCGTTGAG CACCGCCAGG TGCGAATAAG GGACAGTGAA GAAGGAACAC CCGCTCGCGG GTGGGCCTAC TTCACCTATC CTGCCCGGCT GACGCCGTTG GATACACCAA GGAAAGTCTA CACGAACCCT TTGGCAAAAT CCTGTATATC GTGCGAAAAA GGATGGATAT ACCGAAAAAA TCGCTATAAT GACCCCGAAG CAGGGTTATG CAGCGGAAAA GATCCGTCGA TCGACCCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGG GGGATCAGGA CCGCTGCCGG AGCGCAACCC ACTCACTACA GCAGAGCCAT GTAGGGCCGC CGGCGTTGTG GATACCACGC GGAAAACTTG GCCCTCACTG ACAGATGAGG GGCGGACGTT GACACTTGAG GGGCCGACTC ACCCGGCGCG GCGTTGACAG ATGAGGGGCA GGCTCGATTT CGGCCGGCGA CGTGGAGCTG GCCAGCCTCG CAAATCGGCG AAAACGCCTG ATTTTACGCG AGTTTCCCAC AGATGATGTG GACAAGCCTG GGGATAAGTG CCCTGCGGTA TTGACACTTG AGGGGCGCGA CTACTGACAG ATGAGGGGCG CGATCCTTGA CACTTGAGGG GCAGAGTGAT GACAGATGAG GGGCGCACCT ATTGACATTT GAGGGGCTGT CCACAGGCAG AAAATCCAGC ATTTGCAAGG GTTTCCGCCC GTTTTTCGGC CACCGCTAAC CTGTCTTTTA ACCTGCTTTT AAACCAATAT TTATAAACCT TGTTTTTAAC CAGGGCTGCG CCCTGGCGCG TGACCGCGCA CGCCGAAGGG GGGTGCCCCC CCTTCTCGAA CCCTCCCGGC CCGCTAACGC GGCACCCCAT CCCCCCAGGG GCTGCGCCCC TCGGCCGCGA ACGACCTCAC CCCAAAAATG GCAGCCACGT AGAAAGCCAG TCCGCAGAAA CGGTGCTGAC CCCGGATGAA TGTCAGCTAC TGGGCTATCT GGACAAGGGA AAACGCAAGC GCAAAGAGAA AGCAGGTAGC TTGCAGTGGG CTTACATGGC GATAGCTAGA CTGGGCGGTT TTATGGACAG CAAGCGAACC GGAATTGCCA GCTGGGGCGC CCTCTGGTAA GGTTGGGAAG CCCTGCAAAG TAAACTGGAT GGCTTTCTTG CCGCCAAGGA TCTGATGGCG CAGGGGATCA AGATCGACGG ATCGATCCGG GGAATTAATT CCGGGGCAAT CCCGCAAGGA GGGTGAATGA ATCGGACGTT TGACCGGAAG GCATACAGGC AAGAACTGAT CGACGCGGGG TTTTCCGCCG AGGATGCCGA AACCATCGCA AGCCGCACCG TCATGCGTGC GCCCCGCGAA ACCTTCCAGT CCGTCGGCTC GATGGTCCAG CAAGCTACGG CCAAGATCGA GCGCGACAGC GTGCAACTGG CTCCCCCTGC CCTGCCCGCG CCATCGGCCG CCGTGGAGCG TTCGCGTCGT CTCGAACAGG AGGCGGCAGG TTTGGCGAAG TCGATGACCA TCGACACGCG AGGAACTATG ACGACCAAGA AGCGAAAAAC CGCCGGCGAG GACCTGGCAA AACAGGTCAG CGAGGCCAAG CAGGCCGCGT TGCTGAAACA CACGAAGCAG CAGATCAAGG AAATGCAGCT TTCCTTGTTC GATATTGCGC CGTGGCCGGA CACGATGCGA GCGATGCCAA ACGACACGGC CCGCTCTGCC CTGTTCACCA CGCGCAACAA GAAAATCCCG CGCGAGGCGC TGCAAAACAA GGTCATTTTC CACGTCAACA AGGACGTGAA GATCACCTAC ACCGGCGTCG AGCTGCGGGC CGACGATGAC GAACTGGTGT GGCAGCAGGT GTTGGAGTAC GCGAAGCGCA CCCCTATCGG CGAGCCGATC ACCTTCACGT TCTACGAGCT TTGCCAGGAC CTGGGCTGGT CGATCAATGG CCGGTATTAC ACGAAGGCCG AGGAATGCCT GTCGCGCCTA CAGGCGACGG CGATGGGCTT CACGTCCGAC CGCGTTGGGC ACCTGGAATC GGTGTCGCTG CTGCACCGCT TCCGCGTCCT GGACCGTGGC AAGAAAACGT CCCGTTGCCA GGTCCTGATC GACGAGGAAA TCGTCGTGCT GTTTGCTGGC GACCACTACA CGAAATTCAC ATGGGAGAAG TACCGCAAGC TGTCGCCGAC GGCCCGACGG ATGTTCGACT ATTTCAGCTC GCACCGGGAG CCGTACCCGC TCAAGCTGGA AACCTTCCGC CTCATGTGCG GATCGGATTC CACCCGCGTG AAGAAGTGGC GCGAGCAGGT CGGCGAAGCC TGCGAAGAGT TGCGAGGCAG CGGCCTGGTG GAACACGCCT GGGTCAATGA TGACCTGGTG CATTGCAAAC GCTAGGGCCT TGTGGGGTCA GTTCCGGCTG GGGGTTCAGC AGCCACCGGC ATGCAAGCTT GCTAGAGGGT CAGCTTTATG CTTGTAAACC GTTTTGTGAA AAAATTTTTA AAATAAAAAA GGGGACCTCT AGGGTCCCCA ATTAATTAGT AATATAATCT ATTAAAGGTC ATTCAAAAGG TCATCCACCG GATCAATTCC CCTGCTCGCG CAGGCTGGGT GCCAAGCTCT CGGGTAACAT CAAGGCCCGA TCCTTGGTAG TCGGCAAATA ATGTCTAACA ATTCGTTCAA GCCGACGCCG CTTCGCGGCG CGGCTTAACT CAAGCGTTAG ATGCACTAAG CACATAATTG CTCACAGCCA AACTATCAGG TCAAGTCTGC TTTTATTATT TTTAAGCGTG CATAATAAGC CCTACACAAA TTGGGAGATA TATCATGAAA GGCTGGCTTT TTCTTGTTAT CGCAATAGTT GGCGAAGTAA TCGCAACATC CGCATTAAAA TCTAGCGAGG GCTTTACTAA GCTGATCCGG TGGATGACCT TTTGAATGAC CTTTAATAGA TTATATTACT AATTAATTGG GGACCCTAGA GGTCCCCTTT TTTATTTTAA AAATTTTTTC ACAAAACGGT TTACAAGCAT AAAGCTGACT CTAGCTAGAG GATCCCCGGG TACCGAGCTC GAATTCATAT GTTCATGACT CCATTATTAT TGTTTCTGTT GCATAAAGCC TAAGGGGTAG GCCTTTCTAG AGATAGCCAT TTTTTGCACT CCTGTATCCG CTTCTTGCAA GGCTGGACTT ATCCCTATCA AACCGGACAC GTTTATGCTG GTTATGCTGG CTGCATGGGG CATTAGTTGG GGAGCCAGAT TTGTCATGGA GCAGGCCGTT CTGCTTTATG GATCAGGAAA AAACTATTTG TTCTTCAGTC ATGGTACTGT TCTGATGTAC CTGCTGTGTG TTTTCCTGGT ATACCGCCGT TGGATAGCTC CGCTACCGGT CGTTGGTCAG ATCAAGCTAA TTCCCATGCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGTA ATTACGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAG
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