pKD4-2×ME-Tac-EGFP-Mob
$128.00
In stock
SKU
PL60934
100μg liquid plasmid(Instantly):$163
| name | pKD4-2×ME-Tac-EGFP-Mob | another name | apKD4-2×ME-Tac-EGFP-Mob |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | Red |
| Fragment species | Prokaryotic resistance | Amp,Kan | |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | Tac | Replicon | R6K |
| Competence | S17-1λpir | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | rrnBT1-F：GTTGTTTGTCGGTGAACGCTC | |
| 3′ sequencing primer | PKD-R：CAGTGACACAGGAACACTT | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATTGCAGC ATTACACGTC CTGTCTCTTA TACACATCTT TGAGCGATTG TGTAGGCTGG AGCTGCTTCG AAGTTCCTAT ACTTTCTAGA GAATAGGAAC TTCGGAATAG GAACTTCAAG ATCCCCTCAC GCTGCCGCAA GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGAACACG TAGAAAGCCA GTCCGCAGAA ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAGCTT CAAAAGCGCT CTGAAGTTCC TATACTTTCT AGAGAATAGG AACTTCGGAA TAGGAACTAA GGAGGATATT TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTCA CCTTGATGCC GTTCTTCTGC TTGTCGGCCA TGATATAGAC GTTGTGGCTG TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC GTAGGTGGCA TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT GAATACTGTT TCCTGTGTGA AATTCCACAC ATTATACGAG CCGATGATTA ATTGTCAACA TATGGACCAT AGATGTGTAT AAGAGACAGG GCTAATTCCC ATGTCAGCCG TTAAGTGTTC CTGTGTCACT GAAAATTGCT TTGAGAGGCT CTAAGGGCTT CTCAGTGCGT TACATCCCTG GCTTGTTGTC CACAACCGTT AAACCTTAAA AGCTTTAAAA GCCTTATATA TTCTTTTTTT TCTTATAAAA CTTAAAACCT TAGAGGCTAT TTAAGTTGCT GATTTATATT AATTTTATTG TTCAAACATG AGAGCTTAGT ACGTGAAACA TGAGAGCTTA GTACGTTAGC CATGAGAGCT TAGTACGTTA GCCATGAGGG TTTAGTTCGT TAAACATGAG AGCTTAGTAC GTTAAACATG AGAGCTTAGT ACGTGAAACA TGAGAGCTTA GTACGTACTA TCAACAGGTT GAACTGCGGA TCTTGCGGCC GCAAAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGCATC GAGCCGACCA GGCTTTCCAC GCCCGCGTGC CGCTCCATGT CGTTCGCGCG GTTCTCGGAA ACGCGCTGCC GCGTTTCGTG ATTGTCACGC TCAAGCCCGT AGTCCCGTTC GAGCGTCGCG CAGAGGTCAG CGAGGGCGCG GTAGGCCCGA TACGGCTCAT GGATGGTGTT TCGGGTCGGG TGAATCTTGT TGATGGCGAT ATGGATGTGC AGGTTGTCGG TGTCGTGATG CACGGCACTG ACGCGCTGAT GCTCGGCGAA GCCAAGCCCA GCGCAGATGC GGTCCTCAAT CGCGCGCAAC GTCTCCGCGT CGGGCTTCTC TCCCGCGCGG AAGCTAACCA GCAGGTGATA GGTCTTGTCG GCCTCGGAAC GGGTGTTGCC GTGCTGGGTC GCCATCACCT CGGCCATGAC AGCGGGCAGG GTGTTTGCCT CGCAGTTCGT GACGCGCACG TGACCCAGGC GCTCGGTCTT GCCTTGCTCG TCGGTGATGT ACTTCACCAG CTCCGCGAAG TCGCTCTTCT TGATGGAGCG CATGGGGACG TGCTTGGCAA TCACGCGCAC CCCCCGGCCG TTTTAGCGGC TAAAAAAGTC ATGGCTCTGC CCTCGGGCGG ACCACGCCCA TCATGACCTT GCCAAGCTCG TCCTGCTTCT CTTCGATCTT CGCCAGCAGG GCGAGGATCG TGGCATCACC GAACCGCGCC GTGCGCGGGT CGTCGGTGAG CCAGAGTTTC AGCAGGCCGC CCAGGCGGCC CAGGTCGCCA TTGATGCGGG CCAGCTCGCG GACGTGCTCA TAGTCCACGA CGCCCGTGAT TTTGTAGCCC TGGCCGACGG CCAGCAGGTA GGCCGACAGG CTCATGCCGG CCGCCGCCGC CTTTTCCTCA ATCGCTCTTC GTTCGTCTGG AAGGCAGTAC ACCTTGATAG GTGGGCTGCC CTTCCTGGTT GGCTTGGTTT CATCAGCCAT CCGCTTGCCC TCATCTGTTA CGCCGGCGGT AGCCGGCCAG CCTCGCAGAG CAGGATTCCC GTTGAGCACC GCCAGGTGCG AATAAGGGAC AGTGAAGAAG GAACACCCGC TCGCGGGTGG GCCTACTTCA CCTATCCTGC CCGGCTGACG CCGTTGGATA CACCAAGGAA AGTCTACACG AACCCTTTGG CAAAATCCTG TATATCGTGC GAAAAAGGAT GGATATACCG AAAAAATCGC TATAATGACC CCGAAGCAGG GTTATGCAGC GGAAAAGCGC TGCTTCCCTG CTGTTTTGTG GAATATCTAC CGACTGGAAA CAGGCAAATG CAGGAAATTA CTGAACTGAG GGGACAGGCG AGAGACGATG CCAAAGAGCT ACACCGACGA GCTGGCCGAG TGGGTTGAAT CCCGCGCGGC CAAGAAGCGC CGGCGTGATG AGGCTGCGGT TGCGTTCCTG GCGGTGAGGG CGGATGTCGA GGCGGCGTTA GCGTCCGGCT ATGCGCTCGT CACCATTTGG GAGCACATGC GGGAAACGGG GAAGGTCAAG TTCTCCTACG AGACGTTCCG CTCGCACGCC AGGCGGCACA TCAAGGCCAA GCCCGCCGAT GTGCCCGCAC CGCAGGCCAA GGCTGCGGAA CCCGCGCCGG CACCCAAGAC GCCGGAGCCA CGGCGGCCGA AGCAGGGGGG CAAGGCTGAA AAGCCGGCCC CCGCTGCGGC CCCGACCGGC TTCACCTTCA ACCCAACACC GGACAAAAAA TGGCCCCCCG ATGGTAGTGT GGGGTCTCCC CATGCGAGAG TAGGGAACTG CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA TTAAGCAGAA GGCCATCCTG ACGGATGGCC TTTTTGCGTG GCCAGTGCCA AGCTTGCATG C
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