pCDNA3.1-3×FLAG-TET2(human)
$142.00
In stock
SKU
PL60910
NM_001127208.3
100μg liquid plasmid(Instantly):$163
| name | pCDNA3.1-3×FLAG-TET2(human) | another name | apCDNA3.1-3×FLAG-TET2(human) |
| Insert Size (bp) | 6009bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG CCACCATGGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAAG ACGATGACGA CAAGATGGAA CAGGATAGAA CCAACCATGT TGAGGGCAAC AGACTAAGTC CATTCCTGAT ACCATCACCT CCCATTTGCC AGACAGAACC TCTGGCTACA AAGCTCCAGA ATGGAAGCCC ACTGCCTGAG AGAGCTCATC CAGAAGTAAA TGGAGACACC AAGTGGCACT CTTTCAAAAG TTATTATGGA ATACCCTGTA TGAAGGGAAG CCAGAATAGT CGTGTGAGTC CTGACTTTAC ACAAGAAAGT AGAGGGTATT CCAAGTGTTT GCAAAATGGA GGAATAAAAC GCACAGTTAG TGAACCTTCT CTCTCTGGGC TCCTTCAGAT CAAGAAATTG AAACAAGACC AAAAGGCTAA TGGAGAAAGA CGTAACTTCG GGGTAAGCCA AGAAAGAAAT CCAGGTGAAA GCAGTCAACC AAATGTCTCC GATTTGAGTG ATAAGAAAGA ATCTGTGAGT TCTGTAGCCC AAGAAAATGC AGTTAAAGAT TTCACCAGTT TTTCAACACA TAACTGCAGT GGGCCTGAAA ATCCAGAGCT TCAGATTCTG AATGAGCAGG AGGGGAAAAG TGCTAATTAC CATGACAAGA ACATTGTATT ACTTAAAAAC AAGGCAGTGC TAATGCCTAA TGGTGCTACA GTTTCTGCCT CTTCCGTGGA ACACACACAT GGTGAACTCC TGGAAAAAAC ACTGTCTCAA TATTATCCAG ATTGTGTTTC CATTGCGGTG CAGAAAACCA CATCTCACAT AAATGCCATT AACAGTCAGG CTACTAATGA GTTGTCCTGT GAGATCACTC ACCCATCGCA TACCTCAGGG CAGATCAATT CCGCACAGAC CTCTAACTCT GAGCTGCCTC CAAAGCCAGC TGCAGTGGTG AGTGAGGCCT GTGATGCTGA TGATGCTGAT AATGCCAGTA AACTAGCTGC AATGCTAAAT ACCTGTTCCT TTCAGAAACC AGAACAACTA CAACAACAAA AATCAGTTTT TGAGATATGC CCATCTCCTG CAGAAAATAA CATCCAGGGA ACCACAAAGC TAGCGTCTGG TGAAGAATTC TGTTCAGGTT CCAGCAGCAA TTTGCAAGCT CCTGGTGGCA GCTCTGAACG GTATTTAAAA CAAAATGAAA TGAATGGTGC TTACTTCAAG CAAAGCTCAG TGTTCACTAA GGATTCCTTT TCTGCCACTA CCACACCACC ACCACCATCA CAATTGCTTC TTTCTCCCCC TCCTCCTCTT CCACAGGTTC CTCAGCTTCC TTCAGAAGGA AAAAGCACTC TGAATGGTGG AGTTTTAGAA GAACACCACC ACTACCCCAA CCAAAGTAAC ACAACACTTT TAAGGGAAGT GAAAATAGAG GGTAAACCTG AGGCACCACC TTCCCAGAGT CCTAATCCAT CTACACATGT ATGCAGCCCT TCTCCGATGC TTTCTGAAAG GCCTCAGAAT AATTGTGTGA ACAGGAATGA CATACAGACT GCAGGGACAA TGACTGTTCC ATTGTGTTCT GAGAAAACAA GACCAATGTC AGAACACCTC AAGCATAACC CACCAATTTT TGGTAGCAGT GGAGAGCTAC AGGACAACTG CCAGCAGTTG ATGAGAAACA AAGAGCAAGA GATTCTGAAG GGTCGAGACA AGGAGCAAAC ACGAGATCTT GTGCCCCCAA CACAGCACTA TCTGAAACCA GGATGGATTG AATTGAAGGC CCCTCGTTTT CACCAAGCGG AATCCCATCT AAAACGTAAT GAGGCATCAC TGCCATCAAT TCTTCAGTAT CAACCCAATC TCTCCAATCA AATGACCTCC AAACAATACA CTGGAAATTC CAACATGCCT GGGGGGCTCC CAAGGCAAGC TTACACCCAG AAAACAACAC AGCTGGAGCA CAAGTCACAA ATGTACCAAG TTGAAATGAA TCAAGGGCAG TCCCAAGGTA CAGTGGACCA ACATCTCCAG TTCCAAAAAC CCTCACACCA GGTGCACTTC TCCAAAACAG ACCATTTACC AAAAGCTCAT GTGCAGTCAC TGTGTGGCAC TAGATTTCAT TTTCAACAAA GAGCAGATTC CCAAACTGAA AAACTTATGT CCCCAGTGTT GAAACAGCAC TTGAATCAAC AGGCTTCAGA GACTGAGCCA TTTTCAAACT CACACCTTTT GCAACATAAG CCTCATAAAC AGGCAGCACA AACACAACCA TCCCAGAGTT CACATCTCCC TCAAAACCAG CAACAGCAGC AAAAATTACA AATAAAGAAT AAAGAGGAAA TACTCCAGAC TTTTCCTCAC CCCCAAAGCA ACAATGATCA GCAAAGAGAA GGATCATTCT TTGGCCAGAC TAAAGTGGAA GAATGTTTTC ATGGTGAAAA TCAGTATTCA AAATCAAGCG AGTTCGAGAC TCATAATGTC CAAATGGGAC TGGAGGAAGT ACAGAATATA AATCGTAGAA ATTCCCCTTA TAGTCAGACC ATGAAATCAA GTGCATGCAA AATACAGGTT TCTTGTTCAA ACAATACACA CCTAGTTTCA GAGAATAAAG AACAGACTAC ACATCCTGAA CTTTTTGCAG GAAACAAGAC CCAAAACTTG CATCACATGC AATATTTTCC AAATAATGTG ATCCCAAAGC AAGATCTTCT TCACAGGTGC TTTCAAGAAC AGGAGCAGAA GTCACAACAA GCTTCAGTTC TACAGGGATA TAAAAATAGA AACCAAGATA TGTCTGGTCA ACAAGCTGCG CAACTTGCTC AGCAAAGGTA CTTGATACAT AACCATGCAA ATGTTTTTCC TGTGCCTGAC CAGGGAGGAA GTCACACTCA GACCCCTCCC CAGAAGGACA CTCAAAAGCA TGCTGCTCTA AGGTGGCATC TCTTACAGAA GCAAGAACAG CAGCAAACAC AGCAACCCCA AACTGAGTCT TGCCATAGTC AGATGCACAG GCCAATTAAG GTGGAACCTG GATGCAAGCC ACATGCCTGT ATGCACACAG CACCACCAGA AAACAAAACA TGGAAAAAGG TAACTAAGCA AGAGAATCCA CCTGCAAGCT GTGATAATGT GCAGCAAAAG AGCATCATTG AGACCATGGA GCAGCATCTG AAGCAGTTTC ACGCCAAGTC GTTATTTGAC CATAAGGCTC TTACTCTCAA ATCACAGAAG CAAGTAAAAG TTGAAATGTC AGGGCCAGTC ACAGTTTTGA CTAGACAAAC CACTGCTGCA GAACTTGATA GCCACACCCC AGCTTTAGAG CAGCAAACAA CTTCTTCAGA AAAGACACCA ACCAAAAGAA CAGCTGCTTC TGTTCTCAAT AATTTTATAG AGTCACCTTC CAAATTACTA GATACTCCTA TAAAAAATTT ATTGGATACA CCTGTCAAGA CTCAATATGA TTTCCCATCT TGCAGATGTG TAGAGCAAAT TATTGAAAAA GATGAAGGTC CTTTTTATAC CCATCTAGGA GCAGGTCCTA ATGTGGCAGC TATTAGAGAA ATCATGGAAG AAAGGTTTGG ACAGAAGGGT AAAGCTATTA GGATTGAAAG AGTCATCTAT ACTGGTAAAG AAGGCAAAAG TTCTCAGGGA TGTCCTATTG CTAAGTGGGT GGTTCGCAGA AGCAGCAGTG AAGAGAAGCT ACTGTGTTTG GTGCGGGAGC GAGCTGGCCA CACCTGTGAG GCTGCAGTGA TTGTGATTCT CATCCTGGTG TGGGAAGGAA TCCCGCTGTC TCTGGCTGAC AAACTCTACT CGGAGCTTAC CGAGACGCTG AGGAAATACG GCACGCTCAC CAATCGCCGG TGTGCCTTGA ATGAAGAGAG AACTTGCGCC TGTCAGGGGC TGGATCCAGA AACCTGTGGT GCCTCCTTCT CTTTTGGTTG TTCATGGAGC ATGTACTACA ATGGATGTAA GTTTGCCAGA AGCAAGATCC CAAGGAAGTT TAAGCTGCTT GGGGATGACC CAAAAGAGGA AGAGAAACTG GAGTCTCATT TGCAAAACCT GTCCACTCTT ATGGCACCAA CATATAAGAA ACTTGCACCT GATGCATATA ATAATCAGAT TGAATATGAA CACAGAGCAC CAGAGTGCCG TCTGGGTCTG AAGGAAGGCC GTCCATTCTC AGGGGTCACT GCATGTTTGG ACTTCTGTGC TCATGCCCAC AGAGACTTGC ACAACATGCA GAATGGCAGC ACATTGGTAT GCACTCTCAC TAGAGAAGAC AATCGAGAAT TTGGAGGAAA ACCTGAGGAT GAGCAGCTTC ACGTTCTGCC TTTATACAAA GTCTCTGACG TGGATGAGTT TGGGAGTGTG GAAGCTCAGG AGGAGAAAAA ACGGAGTGGT GCCATTCAGG TACTGAGTTC TTTTCGGCGA AAAGTCAGGA TGTTAGCAGA GCCAGTCAAG ACTTGCCGAC AAAGGAAACT AGAAGCCAAG AAAGCTGCAG CTGAAAAGCT TTCCTCCCTG GAGAACAGCT CAAATAAAAA TGAAAAGGAA AAGTCAGCCC CATCACGTAC AAAACAAACT GAAAACGCAA GCCAGGCTAA ACAGTTGGCA GAACTTTTGC GACTTTCAGG ACCAGTCATG CAGCAGTCCC AGCAGCCCCA GCCTCTACAG AAGCAGCCAC CACAGCCCCA GCAGCAGCAG AGACCCCAGC AGCAGCAGCC ACATCACCCT CAGACAGAGT CTGTCAACTC TTATTCTGCT TCTGGATCCA CCAATCCATA CATGAGACGG CCCAATCCAG TTAGTCCTTA TCCAAACTCT TCACACACTT CAGATATCTA TGGAAGCACC AGCCCTATGA ACTTCTATTC CACCTCATCT CAAGCTGCAG GTTCATATTT GAATTCTTCT AATCCCATGA ACCCTTACCC TGGGCTTTTG AATCAGAATA CCCAATATCC ATCATATCAA TGCAATGGAA ACCTATCAGT GGACAACTGC TCCCCATATC TGGGTTCCTA TTCTCCCCAG TCTCAGCCGA TGGATCTGTA TAGGTATCCA AGCCAAGACC CTCTGTCTAA GCTCAGTCTA CCACCCATCC ATACACTTTA CCAGCCAAGG TTTGGAAATA GCCAGAGTTT TACATCTAAA TACTTAGGTT ATGGAAACCA AAATATGCAG GGAGATGGTT TCAGCAGTTG TACCATTAGA CCAAATGTAC ATCATGTAGG GAAATTGCCT CCTTATCCCA CTCATGAGAT GGATGGCCAC TTCATGGGAG CCACCTCTAG ATTACCACCC AATCTGAGCA ATCCAAACAT GGACTATAAA AATGGTGAAC ATCATTCACC TTCTCACATA ATCCATAACT ACAGTGCAGC TCCGGGCATG TTCAACAGCT CTCTTCATGC CCTGCATCTC CAAAACAAGG AGAATGACAT GCTTTCCCAC ACAGCTAATG GGTTATCAAA GATGCTTCCA GCTCTTAACC ATGATAGAAC TGCTTGTGTC CAAGGAGGCT TACACAAATT AAGTGATGCT AATGGTCAGG AAAAGCAGCC ATTGGCACTA GTCCAGGGTG TGGCTTCTGG TGCAGAGGAC AACGATGAGG TCTGGTCAGA CAGCGAGCAG AGCTTTCTGG ATCCTGACAT TGGGGGAGTG GCCGTGGCTC CAACTCATGG GTCAATTCTC ATTGAGTGTG CAAAGCGTGA GCTGCATGCC ACAACCCCTT TAAAGAATCC CAATAGGAAT CACCCCACCA GGATCTCCCT CGTCTTTTAC CAGCATAAGA GCATGAATGA GCCAAAACAT GGCTTGGCTC TTTGGGAAGC CAAAATGGCT GAAAAAGCCC GTGAGAAAGA GGAAGAGTGT GAAAAGTATG GCCCAGACTA TGTGCCTCAG AAATCCCATG GCAAAAAAGT GAAACGGGAG CCTGCTGAGC CACATGAAAC TTCAGAGCCC ACTTACCTGC GTTTCATCAA GTCTCTTGCC GAAAGGACCA TGTCCGTGAC CACAGACTCC ACAGTAACTA CATCTCCATA TGCCTTCACT CGGGTCACAG GGCCTTACAA CAGATATATA TGACTCGAGT CTAGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT
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