pCDNA3.1-3×FLAG-TET1(human)
$142.00
In stock
SKU
PL60906
NM_030625.3
100μg liquid plasmid(Instantly):$163
| name | pCDNA3.1-3×FLAG-TET1(human) | another name | apCDNA3.1-3×FLAG-TET1(human) |
| Insert Size (bp) | 6411bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGCC ACCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAAGAC GATGACGACA AGATGTCTCG ATCCCGCCAT GCAAGGCCTT CCAGATTAGT CAGGAAGGAA GATGTAAACA AAAAAAAGAA AAACAGCCAA CTACGAAAGA CAACCAAGGG AGCCAACAAA AATGTGGCAT CAGTCAAGAC TTTAAGCCCT GGAAAATTAA AGCAATTAAT TCAAGAAAGA GATGTTAAGA AAAAAACAGA ACCTAAACCA CCCGTGCCAG TCAGAAGCCT TCTGACAAGA GCTGGAGCAG CACGCATGAA TTTGGATAGG ACTGAGGTTC TTTTTCAGAA CCCAGAGTCC TTAACCTGCA ATGGGTTTAC AATGGCGCTA CGAAGCACCT CTCTTAGCAG GCGACTCTCC CAACCCCCAC TGGTCGTAGC CAAATCCAAA AAGGTTCCAC TTTCTAAGGG TTTAGAAAAG CAACATGATT GTGATTATAA GATACTCCCT GCTTTGGGAG TAAAGCACTC AGAAAATGAT TCGGTTCCAA TGCAAGACAC CCAAGTCCTT CCTGATATAG AGACTCTAAT TGGTGTACAA AATCCCTCTT TACTTAAAGG TAAGAGCCAA GAGACAACTC AGTTTTGGTC CCAAAGAGTT GAGGATTCCA AGATCAATAT CCCTACCCAC AGTGGCCCTG CAGCTGAGAT CCTTCCTGGG CCACTGGAAG GGACACGCTG TGGTGAAGGA CTATTCTCTG AAGAGACATT GAATGATACC AGTGGTTCCC CAAAAATGTT TGCTCAGGAC ACAGTGTGTG CTCCTTTTCC CCAAAGAGCA ACCCCCAAAG TTACCTCTCA AGGAAACCCC AGCATTCAGT TAGAAGAGTT GGGTTCACGA GTAGAATCTC TTAAGTTATC TGATTCTTAC CTGGATCCCA TTAAAAGTGA ACATGATTGC TACCCCACCT CCAGTCTTAA TAAGGTTATA CCTGACTTGA ACCTTAGAAA CTGCTTGGCT CTTGGTGGGT CTACGTCTCC TACCTCTGTA ATAAAATTCC TCTTGGCAGG CTCAAAACAA GCGACCCTTG GTGCTAAACC AGATCATCAA GAGGCCTTCG AAGCTACTGC AAATCAACAG GAAGTTTCTG ATACCACCTC TTTCCTAGGA CAGGCCTTTG GTGCTATCCC ACATCAATGG GAACTTCCTG GTGCTGACCC AGTTCATGGT GAGGCCCTGG GTGAGACCCC AGATCTACCA GAGATTCCTG GTGCTATTCC AGTCCAAGGA GAGGTCTTTG GTACTATTTT AGACCAACAA GAAACTCTTG GTATGAGTGG GAGTGTTGTC CCAGACTTGC CTGTCTTCCT TCCTGTTCCT CCAAATCCAA TTGCTACCTT TAATGCTCCT TCCAAATGGC CTGAGCCCCA AAGCACTGTC TCATATGGAC TTGCAGTCCA GGGTGCTATA CAGATTTTGC CTTTGGGCTC AGGACACACT CCTCAATCAT CATCAAACTC AGAGAAAAAT TCATTACCTC CAGTAATGGC TATAAGCAAT GTAGAAAATG AGAAGCAGGT TCATATAAGC TTCCTGCCAG CTAACACTCA GGGGTTCCCA TTAGCCCCTG AGAGAGGACT CTTCCATGCT TCACTGGGTA TAGCCCAACT CTCTCAGGCT GGTCCTAGCA AATCAGACAG AGGGAGCTCC CAGGTCAGTG TAACCAGCAC AGTTCATGTT GTCAACACCA CAGTGGTGAC TATGCCAGTG CCAATGGTCA GTACCTCCTC TTCTTCCTAT ACCACTTTGC TACCGACTTT GGAAAAGAAG AAAAGAAAGC GATGTGGGGT CTGTGAACCC TGCCAGCAGA AGACCAACTG TGGTGAATGC ACTTACTGCA AGAACAGAAA GAACAGCCAT CAGATCTGTA AGAAAAGAAA ATGTGAGGAG CTGAAAAAGA AACCATCTGT TGTTGTGCCT CTGGAGGTTA TAAAGGAAAA CAAGAGGCCC CAGAGGGAAA AGAAGCCCAA AGTTTTAAAG GCAGATTTTG ACAACAAACC AGTAAATGGC CCCAAGTCAG AATCCATGGA CTACAGTAGA TGTGGTCATG GGGAAGAACA AAAATTGGAA TTGAACCCAC ATACTGTTGA AAATGTAACT AAAAATGAAG ACAGCATGAC AGGCATCGAG GTGGAGAAGT GGACACAAAA CAAGAAATCA CAGTTAACTG ATCACGTGAA AGGAGATTTT AGTGCTAATG TCCCAGAAGC TGAAAAATCG AAAAACTCTG AAGTTGACAA GAAACGAACC AAATCTCCAA AATTGTTTGT ACAAACCGTA AGAAATGGCA TTAAACATGT ACACTGTTTA CCAGCTGAAA CAAATGTTTC ATTTAAAAAA TTCAATATTG AAGAATTCGG CAAGACATTG GAAAACAATT CTTATAAATT CCTAAAAGAC ACTGCAAACC ATAAAAACGC TATGAGCTCT GTTGCTACTG ATATGAGTTG TGATCATCTC AAGGGGAGAA GTAACGTTTT AGTATTCCAG CAGCCTGGCT TTAACTGCAG TTCCATTCCA CATTCTTCAC ACTCCATCAT AAATCATCAT GCTAGTATAC ACAATGAAGG TGATCAACCA AAAACTCCTG AGAATATACC AAGTAAAGAA CCAAAAGATG GATCTCCCGT TCAACCAAGT CTCTTATCGT TAATGAAAGA TAGGAGATTA ACATTGGAGC AAGTGGTAGC CATAGAGGCC CTGACTCAAC TCTCAGAAGC CCCATCAGAG AATTCCTCCC CATCAAAGTC AGAGAAGGAT GAGGAATCAG AGCAGAGAAC AGCCAGTTTG CTTAATAGCT GCAAAGCTAT CCTCTACACT GTAAGAAAAG ACCTCCAAGA CCCAAACTTA CAGGGAGAGC CACCAAAACT TAATCACTGT CCATCTTTGG AAAAACAAAG TTCATGCAAC ACGGTGGTTT TCAATGGGCA AACTACTACC CTTTCCAACT CACATATCAA CTCAGCTACT AACCAAGCAT CCACAAAGTC ACATGAATAT TCAAAAGTCA CAAATTCATT ATCTCTTTTT ATACCAAAAT CAAATTCATC CAAGATTGAC ACCAATAAAA GTATTGCTCA AGGGATAATT ACTCTTGACA ATTGTTCCAA TGATTTGCAT CAGTTGCCAC CAAGAAATAA TGAAGTGGAG TATTGCAACC AGTTACTGGA CAGCAGCAAA AAATTGGACT CAGATGATCT ATCATGTCAG GATGCAACCC ATACCCAAAT TGAGGAAGAT GTTGCAACAC AGTTGACACA ACTTGCTTCG ATAATTAAGA TCAATTATAT AAAACCAGAG GACAAAAAAG TTGAAAGTAC ACCAACAAGC CTTGTCACAT GTAATGTACA GCAAAAATAC AATCAGGAGA AGGGCACAAT ACAACAGAAA CCACCTTCAA GTGTACACAA TAATCATGGT TCATCATTAA CAAAACAAAA GAACCCAACC CAGAAAAAGA CAAAATCCAC CCCATCAAGA GATCGGCGGA AAAAGAAGCC CACAGTTGTA AGTTATCAAG AAAATGATCG GCAGAAGTGG GAAAAGTTGT CCTATATGTA TGGCACAATA TGCGACATTT GGATAGCATC GAAATTTCAA AATTTTGGGC AATTTTGTCC ACATGATTTT CCTACTGTAT TTGGGAAAAT TTCTTCCTCG ACCAAAATAT GGAAACCACT GGCTCAAACG AGGTCCATTA TGCAACCCAA AACAGTATTT CCACCACTCA CTCAGATAAA ATTACAGAGA TATCCTGAAT CAGCAGAGGA AAAGGTGAAG GTTGAACCAT TGGATTCACT CAGCTTATTT CATCTTAAAA CGGAATCCAA CGGGAAGGCA TTCACTGATA AAGCTTATAA TTCTCAGGTA CAGTTAACGG TGAATGCCAA TCAGAAAGCC CATCCTTTGA CCCAGCCCTC CTCTCCACCT AACCAGTGTG CTAACGTGAT GGCAGGCGAT GACCAAATAC GGTTTCAGCA GGTTGTTAAG GAGCAACTCA TGCATCAGAG ACTGCCAACA TTGCCTGGTA TCTCTCATGA AACACCCTTA CCGGAGTCAG CACTAACTCT CAGGAATGTA AATGTAGTGT GTTCAGGTGG AATTACAGTG GTTTCTACCA AAAGTGAAGA GGAAGTCTGT TCATCCAGTT TTGGAACATC AGAATTTTCC ACAGTGGACA GTGCACAGAA AAATTTTAAT GATTATGCCA TGAACTTCTT TACTAACCCT ACAAAAAACC TAGTGTCTAT AACTAAAGAT TCTGAACTGC CCACCTGCAG CTGTCTTGAT CGAGTTATAC AAAAAGACAA AGGCCCATAT TATACACACC TTGGGGCAGG ACCAAGTGTT GCTGCTGTCA GGGAAATCAT GGAGAATAGG TATGGTCAAA AAGGAAACGC AATAAGGATA GAAATAGTAG TGTACACCGG TAAAGAAGGG AAAAGCTCTC ATGGGTGTCC AATTGCTAAG TGGGTTTTAA GAAGAAGCAG TGATGAAGAA AAAGTTCTTT GTTTGGTCCG GCAGCGTACA GGCCACCACT GTCCAACTGC TGTGATGGTG GTGCTCATCA TGGTGTGGGA TGGCATCCCT CTTCCAATGG CCGACCGGCT ATACACAGAG CTCACAGAGA ATCTAAAGTC ATACAATGGG CACCCTACCG ACAGAAGATG CACCCTCAAT GAAAATCGTA CCTGTACATG TCAAGGAATT GATCCAGAGA CTTGTGGAGC TTCATTCTCT TTTGGCTGTT CATGGAGTAT GTACTTTAAT GGCTGTAAGT TTGGTAGAAG CCCAAGCCCC AGAAGATTTA GAATTGATCC AAGCTCTCCC TTACATGAAA AAAACCTTGA AGATAACTTA CAGAGTTTGG CTACACGATT AGCTCCAATT TATAAGCAGT ATGCTCCAGT AGCTTACCAA AATCAGGTGG AATATGAAAA TGTTGCCCGA GAATGTCGGC TTGGCAGCAA GGAAGGTCGT CCCTTCTCTG GGGTCACTGC TTGCCTGGAC TTCTGTGCTC ATCCCCACAG GGACATTCAC AACATGAATA ATGGAAGCAC TGTGGTTTGT ACCTTAACTC GAGAAGATAA CCGCTCTTTG GGTGTTATTC CTCAAGATGA GCAGCTCCAT GTGCTACCTC TTTATAAGCT TTCAGACACA GATGAGTTTG GCTCCAAGGA AGGAATGGAA GCCAAGATCA AATCTGGGGC CATCGAGGTC CTGGCACCCC GCCGCAAAAA AAGAACGTGT TTCACTCAGC CTGTTCCCCG TTCTGGAAAG AAGAGGGCTG CGATGATGAC AGAGGTTCTT GCACATAAGA TAAGGGCAGT GGAAAAGAAA CCTATTCCCC GAATCAAGCG GAAGAATAAC TCAACAACAA CAAACAACAG TAAGCCTTCG TCACTGCCAA CCTTAGGGAG TAACACTGAG ACCGTGCAAC CTGAAGTAAA AAGTGAAACC GAACCCCATT TTATCTTAAA AAGTTCAGAC AACACTAAAA CTTATTCGCT GATGCCATCC GCTCCTCACC CAGTGAAAGA GGCATCTCCA GGCTTCTCCT GGTCCCCGAA GACTGCTTCA GCCACACCAG CTCCACTGAA GAATGACGCA ACAGCCTCAT GCGGGTTTTC AGAAAGAAGC AGCACTCCCC ACTGTACGAT GCCTTCGGGA AGACTCAGTG GTGCCAATGC AGCTGCTGCT GATGGCCCTG GCATTTCACA GCTTGGCGAA GTGGCTCCTC TCCCCACCCT GTCTGCTCCT GTGATGGAGC CCCTCATTAA TTCTGAGCCT TCCACTGGTG TGACTGAGCC GCTAACGCCT CATCAGCCAA ACCACCAGCC CTCCTTCCTC ACCTCTCCTC AAGACCTTGC CTCTTCTCCA ATGGAAGAAG ATGAGCAGCA TTCTGAAGCA GATGAGCCTC CATCAGACGA ACCCCTATCT GATGACCCCC TGTCACCTGC TGAGGAGAAA TTGCCCCACA TTGATGAGTA TTGGTCAGAC AGTGAGCACA TCTTTTTGGA TGCAAATATT GGTGGGGTGG CCATCGCACC TGCTCACGGC TCGGTTTTGA TTGAGTGTGC CCGGCGAGAG CTGCACGCTA CCACTCCTGT TGAGCACCCC AACCGTAATC ATCCAACCCG CCTCTCCCTT GTCTTTTACC AGCACAAAAA CCTAAATAAG CCCCAACATG GTTTTGAACT AAACAAGATT AAGTTTGAGG CTAAAGAAGC TAAGAATAAG AAAATGAAGG CCTCAGAGCA AAAAGACCAG GCAGCTAATG AAGGTCCAGA ACAGTCCTCT GAAGTAAATG AATTGAACCA AATTCCTTCT CATAAAGCAT TAACATTAAC CCATGACAAT GTTGTCACCG TGTCCCCTTA TGCTCTCACA CACGTTGCGG GGCCCTATAA CCATTGGGTC TGAGGTACCG AGCTCGGATC CACTAGTCCA GTGTGGTGGA ATTCTGCAGA TATCCAGCAC AGTGGCGGCC GCTCGAGTCT AGAGGGCCCG TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGT
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