pML-siCHECK2-SIRT1(pig)-3UTR-mut
$84.00
In stock
SKU
PL60666
100μg liquid plasmid(Instantly):$105
| name | pML-siCHECK2-SIRT1(pig)-3UTR-mut | another name | apML-siCHECK2-SIRT1(pig)-3UTR-mut |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Signal report | Fragment type | UTR |
| Fragment species | Pig | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | SV40 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC GAGCAGTAAT TCTAGGCGAT CGCTCGAGGA CTGTTGCTCC CCCTTACCCC TGCACCCCCC ATTAAGTGAG AGCAACAGGC CCTGATTTAG GTTCCAAAGC CAGTAAGTAC ATGTTTCCTG TTGGGAAGAT TTGGTGTAAT AAACACCAAA CTATTAGACG GAGACGAATT ATCGTGTCCT TGTAATACCA GAATAGCTGA TGAAACTAGT TCCTATAAAA AGCTTAGCCT GCCTTAAAAC TAGACATCAA TTTTCTCAGC TGCAGAAGCT TCTAGCCTTT CAAAAAAGTT CATACTTTAT AAAGTTGCAG AGAAGCATTT ATTTTCCAGT CCTTTCCCCA ATGTGCGGCC GCTGGCCGCA ATAAAATATC TTTATTTTCA TTACATCTGT GTGTTGGTTT TTTGTGTGAG GATCTAAATG AGTCTTCGGA CCTCGCGGGG GCCGCTTAAG CGGTGGTTAG GGTTTGTCTG ACGCGGGGGG AGGGGGAAGG AACGAAACAC TCTCATTCGG AGGCGGCTCG GGGTTTGGTC TTGGTGGCCA CGGGCACGCA GAAGAGCGCC GCGATCCTCT TAAGCACCCC CCCGCCCTCC GTGGAGGCGG GGGTTTGGTC GGCGGGTGGT AACTGGCGGG CCGCTGACTC GGGCGGGTCG CGCGCCCCAG AGTGTGACCT TTTCGGTCTG CTCGCAGACC CCCGGGCGGC GCCGCCGCGG CGGCGACGGG CTCGCTGGGT CCTAGGCTCC ATGGGGACCG TATACGTGGA CAGGCTCTGG AGCATCCGCA CGACTGCGGT GATATTACCG GAGACCTTCT GCGGGACGAG CCGGGTCACG CGGCTGACGC GGAGCGTCCG TTGGGCGACA AACACCAGGA CGGGGCACAG GTACACTATC TTGTCACCCG GAGGCGCGAG GGACTGCAGG AGCTTCAGGG AGTGGCGCAG CTGCTTCATC CCCGTGGCCC GTTGCTCGCG TTTGCTGGCG GTGTCCCCGG AAGAAATATA TTTGCATGTC TTTAGTTCTA TGATGACACA AACCCCGCCC AGCGTCTTGT CATTGGCGAA TTCGAACACG CAGATGCAGT CGGGGCGGCG CGGTCCCAGG TCCACTTCGC ATATTAAGGT GACGCGTGTG GCCTCGAACA CCGAGCGACC CTGCAGCGAC CCGCTTAAAA GCTTGGCATT CCGGTACTGT TGGTAAAGCC ACCATGGCCG ATGCTAAGAA CATTAAGAAG GGCCCTGCTC CCTTCTACCC TCTGGAGGAT GGCACCGCTG GCGAGCAGCT GCACAAGGCC ATGAAGAGGT ATGCCCTGGT GCCTGGCACC ATTGCCTTCA CCGATGCCCA CATTGAGGTG GACATCACCT ATGCCGAGTA CTTCGAGATG TCTGTGCGCC TGGCCGAGGC CATGAAGAGG TACGGCCTGA ACACCAACCA CCGCATCGTG GTGTGCTCTG AGAACTCTCT GCAGTTCTTC ATGCCAGTGC TGGGCGCCCT GTTCATCGGA GTGGCCGTGG CCCCTGCTAA CGACATTTAC AACGAGCGCG AGCTGCTGAA CAGCATGGGC ATTTCTCAGC CTACCGTGGT GTTCGTGTCT AAGAAGGGCC TGCAGAAGAT CCTGAACGTG CAGAAGAAGC TGCCTATCAT CCAGAAGATC ATCATCATGG ACTCTAAGAC CGACTACCAG GGCTTCCAGA GCATGTACAC ATTCGTGACA TCTCATCTGC CTCCTGGCTT CAACGAGTAC GACTTCGTGC CAGAGTCTTT CGACAGGGAC AAAACCATTG CCCTGATCAT GAACAGCTCT GGGTCTACCG GCCTGCCTAA GGGCGTGGCC CTGCCTCATC GCACCGCCTG TGTGCGCTTC TCTCACGCCC GCGACCCTAT TTTCGGCAAC CAGATCATCC CCGACACCGC TATTCTGAGC GTGGTGCCAT TCCACCACGG CTTCGGCATG TTCACCACCC TGGGCTACCT GATTTGCGGC TTTCGGGTGG TGCTGATGTA CCGCTTCGAG GAGGAGCTGT TCCTGCGCAG CCTGCAAGAC TACAAAATTC AGTCTGCCCT GCTGGTGCCA ACCCTGTTCA GCTTCTTCGC TAAGAGCACC CTGATCGACA AGTACGACCT GTCTAACCTG CACGAGATTG CCTCTGGCGG CGCCCCACTG TCTAAGGAGG TGGGCGAAGC CGTGGCCAAG CGCTTTCATC TGCCAGGCAT CCGCCAGGGC TACGGCCTGA CCGAGACAAC CAGCGCCATT CTGATTACCC CAGAGGGCGA CGACAAGCCT GGCGCCGTGG GCAAGGTGGT GCCATTCTTC GAGGCCAAGG TGGTGGACCT GGACACCGGC AAGACCCTGG GAGTGAACCA GCGCGGCGAG CTGTGTGTGC GCGGCCCTAT GATTATGTCC GGCTACGTGA ATAACCCTGA GGCCACAAAC GCCCTGATCG ACAAGGACGG CTGGCTGCAC TCTGGCGACA TTGCCTACTG GGACGAGGAC GAGCACTTCT TCATCGTGGA CCGCCTGAAG TCTCTGATCA AGTACAAGGG CTACCAGGTG GCCCCAGCCG AGCTGGAGTC TATCCTGCTG CAGCACCCTA ACATTTTCGA CGCCGGAGTG GCCGGCCTGC CCGACGACGA TGCCGGCGAG CTGCCTGCCG CCGTCGTCGT GCTGGAACAC GGCAAGACCA TGACCGAGAA GGAGATCGTG GACTATGTGG CCAGCCAGGT GACAACCGCC AAGAAGCTGC GCGGCGGAGT GGTGTTCGTG GACGAGGTGC CCAAGGGCCT GACCGGCAAG CTGGACGCCC GCAAGATCCG CGAGATCCTG ATCAAGGCTA AGAAAGGCGG CAAGATCGCC GTGTAATAAT TCTAGAGTCG GGGCGGCCGG CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TAAAATCGAT AAGGATCCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGGC TCGAC
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