pML-siCHECK2-CircHOMER1(pig)-wt
$84.00
In stock
SKU
PL60662
100μg liquid plasmid(Instantly):$105
| name | pML-siCHECK2-CircHOMER1(pig)-wt | another name | apML-siCHECK2-CircHOMER1(pig)-wt |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Signal report | Fragment type | circRNA |
| Fragment species | Pig | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | SV40 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC GAGCAGTAAT TCTAGGCGAT CGCTCGAGAG AAACTACAGT TCAGCAATCA GCAAACATTG GGAGGCTGAA CTGGCTACCC TCAAAGGAAA TAATGCCAAA CTCACCGCAG CCCTGTTGGA GTCCACTGCC AATGTGAAAC AATGGAAACA GCAACTTGCT GCATATCAAG AGGAAGCAGA ACGTCTGCAC AAGCGGGTGA CTGAGCTTGA ATGCGGCCGC TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAGGA TCTAAATGAG TCTTCGGACC TCGCGGGGGC CGCTTAAGCG GTGGTTAGGG TTTGTCTGAC GCGGGGGGAG GGGGAAGGAA CGAAACACTC TCATTCGGAG GCGGCTCGGG GTTTGGTCTT GGTGGCCACG GGCACGCAGA AGAGCGCCGC GATCCTCTTA AGCACCCCCC CGCCCTCCGT GGAGGCGGGG GTTTGGTCGG CGGGTGGTAA CTGGCGGGCC GCTGACTCGG GCGGGTCGCG CGCCCCAGAG TGTGACCTTT TCGGTCTGCT CGCAGACCCC CGGGCGGCGC CGCCGCGGCG GCGACGGGCT CGCTGGGTCC TAGGCTCCAT GGGGACCGTA TACGTGGACA GGCTCTGGAG CATCCGCACG ACTGCGGTGA TATTACCGGA GACCTTCTGC GGGACGAGCC GGGTCACGCG GCTGACGCGG AGCGTCCGTT GGGCGACAAA CACCAGGACG GGGCACAGGT ACACTATCTT GTCACCCGGA GGCGCGAGGG ACTGCAGGAG CTTCAGGGAG TGGCGCAGCT GCTTCATCCC CGTGGCCCGT TGCTCGCGTT TGCTGGCGGT GTCCCCGGAA GAAATATATT TGCATGTCTT TAGTTCTATG ATGACACAAA CCCCGCCCAG CGTCTTGTCA TTGGCGAATT CGAACACGCA GATGCAGTCG GGGCGGCGCG GTCCCAGGTC CACTTCGCAT ATTAAGGTGA CGCGTGTGGC CTCGAACACC GAGCGACCCT GCAGCGACCC GCTTAAAAGC TTGGCATTCC GGTACTGTTG GTAAAGCCAC CATGGCCGAT GCTAAGAACA TTAAGAAGGG CCCTGCTCCC TTCTACCCTC TGGAGGATGG CACCGCTGGC GAGCAGCTGC ACAAGGCCAT GAAGAGGTAT GCCCTGGTGC CTGGCACCAT TGCCTTCACC GATGCCCACA TTGAGGTGGA CATCACCTAT GCCGAGTACT TCGAGATGTC TGTGCGCCTG GCCGAGGCCA TGAAGAGGTA CGGCCTGAAC ACCAACCACC GCATCGTGGT GTGCTCTGAG AACTCTCTGC AGTTCTTCAT GCCAGTGCTG GGCGCCCTGT TCATCGGAGT GGCCGTGGCC CCTGCTAACG ACATTTACAA CGAGCGCGAG CTGCTGAACA GCATGGGCAT TTCTCAGCCT ACCGTGGTGT TCGTGTCTAA GAAGGGCCTG CAGAAGATCC TGAACGTGCA GAAGAAGCTG CCTATCATCC AGAAGATCAT CATCATGGAC TCTAAGACCG ACTACCAGGG CTTCCAGAGC ATGTACACAT TCGTGACATC TCATCTGCCT CCTGGCTTCA ACGAGTACGA CTTCGTGCCA GAGTCTTTCG ACAGGGACAA AACCATTGCC CTGATCATGA ACAGCTCTGG GTCTACCGGC CTGCCTAAGG GCGTGGCCCT GCCTCATCGC ACCGCCTGTG TGCGCTTCTC TCACGCCCGC GACCCTATTT TCGGCAACCA GATCATCCCC GACACCGCTA TTCTGAGCGT GGTGCCATTC CACCACGGCT TCGGCATGTT CACCACCCTG GGCTACCTGA TTTGCGGCTT TCGGGTGGTG CTGATGTACC GCTTCGAGGA GGAGCTGTTC CTGCGCAGCC TGCAAGACTA CAAAATTCAG TCTGCCCTGC TGGTGCCAAC CCTGTTCAGC TTCTTCGCTA AGAGCACCCT GATCGACAAG TACGACCTGT CTAACCTGCA CGAGATTGCC TCTGGCGGCG CCCCACTGTC TAAGGAGGTG GGCGAAGCCG TGGCCAAGCG CTTTCATCTG CCAGGCATCC GCCAGGGCTA CGGCCTGACC GAGACAACCA GCGCCATTCT GATTACCCCA GAGGGCGACG ACAAGCCTGG CGCCGTGGGC AAGGTGGTGC CATTCTTCGA GGCCAAGGTG GTGGACCTGG ACACCGGCAA GACCCTGGGA GTGAACCAGC GCGGCGAGCT GTGTGTGCGC GGCCCTATGA TTATGTCCGG CTACGTGAAT AACCCTGAGG CCACAAACGC CCTGATCGAC AAGGACGGCT GGCTGCACTC TGGCGACATT GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC GCCTGAAGTC TCTGATCAAG TACAAGGGCT ACCAGGTGGC CCCAGCCGAG CTGGAGTCTA TCCTGCTGCA GCACCCTAAC ATTTTCGACG CCGGAGTGGC CGGCCTGCCC GACGACGATG CCGGCGAGCT GCCTGCCGCC GTCGTCGTGC TGGAACACGG CAAGACCATG ACCGAGAAGG AGATCGTGGA CTATGTGGCC AGCCAGGTGA CAACCGCCAA GAAGCTGCGC GGCGGAGTGG TGTTCGTGGA CGAGGTGCCC AAGGGCCTGA CCGGCAAGCT GGACGCCCGC AAGATCCGCG AGATCCTGAT CAAGGCTAAG AAAGGCGGCA AGATCGCCGT GTAATAATTC TAGAGTCGGG GCGGCCGGCC GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGGCTC GAC
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