NM_152586.4;bA137L10.3; bA137L10.4; C10orf29
100μg liquid plasmid(Instantly):$178
| name | pCDNA3.1-USP54(human)-3×FLAG-SV40-Puro(1synonymous mutations) | another name | apCDNA3.1-USP54(human)-3×FLAG-SV40-Puro(1synonymous mutations) |
| Insert Size (bp) | 5052bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC AAGCTTTCTA GAGCCACCAT GTCTTGGAAG AGAAATTATT TTTCAGGGGG TCGTGGTAGT GTACAAGGGA TGTTTGCACC TCGAAGCTCA ACCTCCATAG CCCCCAGCAA AGGCCTCAGC AATGAGCCAG GGCAAAACAG CTGCTTCCTC AACAGTGCCC TGCAGGTTTT GTGGCACTTG GATATCTTCC GACGTAGCTT TAGGCAGCTT ACAACTCACA AGTGCATGGG AGATTCCTGC ATCTTTTGCG CTCTCAAGGG AATCTTTAAC CAGTTTCAGT GTAGTAGTGA AAAAGTGCTT CCATCTGACA CTCTCCGCAG TGCTCTGGCA AAGACTTTCC AGGATGAACA ACGTTTCCAG CTGGGAATTA TGGATGATGC TGCAGAGTGC TTTGAAAACC TCCTGATGAG AATTCACTTC CACATTGCTG ATGAAACCAA AGAGGATATA TGTACTGCCC AACACTGCAT TTCCCATCAG AAATTTGCAA TGACATTGTT TGAGCAGTGT GTATGTACTA GCTGTGGTGC CACTTCTGAT CCGCTGCCTT TCATCCAGAT GGTACATTAT ATCTCCACCA CTTCCCTTTG CAATCAGGCT ATTTGTATGC TGGAAAGACG AGAGAAACCT TCACCAAGCA TGTTTGGTGA GCTGCTGCAG AATGCCAGCA CCATGGGGGA TCTGCGGAAC TGTCCAAGCA ACTGTGGAGA GAGGATCAGG ATTCGCCGTG TGTTGATGAA TGCTCCACAG ATTATCACGA TTGGGCTGGT ATGGGACTCA GACCACTCAG ACTTAGCAGA AGATGTTATC CACAGCCTGG GAACCTGCCT TAAGCTGGGT GATCTGTTTT TCAGAGTGAC GGATGACCGG GCCAAGCAAT CTGAACTGTA CTTAGTTGGA ATGATCTGTT ACTATGGCAA ACATTATTCT ACATTCTTTT TTCAAACAAA GATTCGCAAA TGGATGTATT TTGATGATGC TCATGTCAAG GAGATTGGGC CCAAATGGAA GGATGTGGTG ACCAAATGCA TCAAGGGGCA TTATCAGCCC CTGCTGCTGC TTTATGCAGA TCCCCAGGGT ACCCCAGTTT CCACCCAGGA CCTGCCTCCC CAAGCTGAGT TCCAGTCATA CAGCAGGACA TGCTACGACA GTGAAGATTC AGGGAGGGAG CCCTCCATCT CAAGTGACAC TCGAACAGAT TCCTCAACGG AGAGCTATCC CTACAAACAT TCCCACCATG AGTCTGTGGT CAGTCACTTC TCTTCTGATT CTCAGGGGAC AGTCATCTAT AATGTGGAAA ATGATTCCAT GTCTCAGAGC AGTCGGGACA CAGGACACCT GACTGATAGT GAATGTAATC AGAAACACAC ATCCAAGAAA GGGTCACTGA TAGAGCGCAA GAGGAGCTCT GGTCGGGTTA GGAGGAAAGG CGATGAGCCC CAGGCCTCGG GATACCACAG TGAAGGAGAA ACACTGAAAG AGAAGCAGGC TCCTAGAAAT GCCTCCAAAC CATCCAGCAG CACCAACAGG CTGAGAGATT TTAAAGAGAC AGTCAGCAAT ATGATCCATA ACAGACCATC CCTGGCTTCT CAGACCAATG TAGGCTCTCA CTGCAGGGGC AGAGGAGGAG ACCAGCCTGA CAAAAAACCT CCTAGGACCC TGCCTTTACA CTCTCGTGAC TGGGAAATAG AGAGTACCAG CAGTGAGTCA AAATCCAGTT CTTCCAGCAA GTATCGTCCC ACATGGAGAC CCAAACGAGA ATCTCTGAAT ATTGACAGTA TCTTTAGTAA GGACAAAAGG AAGCACTGTG GCTATACCCA GCTTAGCCCC TTTTCTGAGG ATTCAGCTAA AGAATTTATA CCAGATGAAC CAAGCAAGCC ACCTTCTTAC GACATTAAAT TTGGTGGACC AAGCCCCCAG TACAAGCGCT GGGGCCCAGC ACGGCCAGGC TCTCACCTTT TAGAGCAGCA CCCCCGACTA ATCCAGCGAA TGGAATCTGG CTATGAAAGC AGTGAGAGGA ACAGCAGCAG CCCTGTCAGC CTGGATGCAG CCCTGCCTGA GAGCTCAAAT GTCTACAGGG ATCCAAGTGC TAAGAGATCA GCTGGGTTGG TTCCTTCCTG GCGTCATATC CCAAAGTCGC ACAGCAGTAG CATCCTGGAG GTAGACTCCA CAGCATCCAT GGGTGGCTGG ACAAAGAGTC AGCCTTTCTC TGGTGAGGAG ATATCTTCTA AAAGTGAACT GGATGAATTG CAGGAAGAGG TGGCCAGGAG GGCGCAGGAA CAGGAACTTC GAAGAAAACG GGAGAAGGAG TTAGAGGCAG CGAAAGGGTT TAACCCTCAT CCTAGCCGCT TCATGGACTT GGATGAACTG CAGAATCAGG GGAGGAGTGA CGGCTTTGAG AGGTCCCTGC AAGAGGCAGA GTCAGTGTTT GAAGAGTCAC TACATCTGGA ACAGAAAGGA GACTGTGCTG CAGCTTTGGC TCTCTGTAAT GAAGCTATCT CTAAACTAAG ACTTGCCCTG CATGGTGCCA GCTGTAGCAC GCACAGCAGA GCCCTAGTCG ATAAGAAGTT GCAAATCAGT ATTCGAAAAG CACGGAGCCT GCAGGATCGC ATGCAGCAGC AGCAATCACC ACAGCAGCCG TCGCAGCCCT CAGCCTGCCT CCCAACACAG GCGGGGACTC TCTCTCAGCC AACAAGTGAA CAGCCTATCC CGCTCCAAGT ATTGTTAAGC CAAGAGGCCC AACTGGAATC CGGCATGGAT ACAGAGTTTG GGGCCAGTTC TTTCTTCCAT TCACCTGCTT CCTGCCATGA GTCACACTCA TCACTATCTC CAGAGTCATC TGCCCCACAG CACAGCTCCC CCAGTAGATC TGCCTTGAAG CTTCTGACTT CGGTTGAAGT AGACAACATT GAACCCTCTG CATTCCACAG GCAAGGTTTA CCTAAAGCAC CAGGGTGGAC TGAGAAGAAT TCTCATCATA GTTGGGAGCC ATTGGATGCC CCAGAGGGTA AGCTGCAAGG CTCTAGGTGT GACAACAGCA GTTGCAGCAA GCTCCCTCCA CAAGAAGGAA GAGGCATTGC TCAAGAACAG CTGTTCCAAG AAAAGAAGGA TCCTGCTAAC CCCTCCCCGG TGATGCCTGG AATAGCCACC TCTGAGAGGG GTGATGAACA CAGCCTAGGC TGTAGTCCTT CAAATTCATC AGCTCAGCCC AGCCTTCCCC TGTATAGAAC CTGCCACCCC ATAATGCCTG TTGCTTCTTC ATTTGTGCTT CACTGTCCTG ATCCTGTGCA GAAAACTAAC CAATGCCTCC AAGGCCAAAG CCTCAAAACT TCATTGACTT TAAAAGTGGA CAGAGGCAGT GAGGAGACCT ATAGGCCAGA GTTTCCCAGC ACAAAGGGGC TTGTCCGTTC TCTGGCTGAG CAGTTCCAGA GGATGCAGGG TGTCTCCATG AGGGATAGTA CAGGTTTCAA GGATAGAAGT TTGTCAGGTA GTCTAAGGAA GAACTCTTCC CCTTCTGATT CTAAGCCTCC TTTCTCACAG GGTCAAGAGA AAGGCCACTG GCCATGGGCA AAGCAACAAT CCTCTCTGGA GGGTGGGGAT AGACCACTTT CCTGGGAAGA GTCCACTGAA CATTCTTCTC TTGCCTTAAA CTCTGGGCTG CCTAATGGTG AAACTTCTAG CGGAGGACAG CCCAGGTTGG CAGAGCCAGA CATATACCAA GAGAAGCTGT CCCAAGTGAG AGATGTTAGG TCTAAGGATC TGGGCAGCAG TACTGACTTG GGGACTTCCT TGCCTTTGGA TTCCTGGGTG AATATCACAA GGTTCTGTGA TTCTCAGCTT AAGCATGGGG CACCTAGGCC AGGAATGAAG TCCTCCCCTC ATGATTCCCA TACGTGTGTA ACCTATCCAG AGAGAAATCA CATCCTTTTG CATCCACATT GGAACCAAGA CACAGAGCAG GAGACCTCAG AATTGGAGTC TCTGTATCAG GCCAGTCTTC AGGCTTCTCA AGCTGGCTGT TCTGGATGGG GGCAGCAGGA TACCGCCTGG CACCCACTTA GCCAAACAGG CTCTGCAGAT GGCATGGGGA GGAGGTTGCA CTCAGCCCAT GATCCTGGTC TCTCAAAGAC TTCAACAGCA GAAATGGAGC ATGGTCTCCA TGAAGCCAGA ACAGTGCGTA CTTCTCAGGC TACACCTTGC CGAGGCCTCA GCAGGGAGTG TGGGGAGGAT GAGCAGTACA GTGCAGAGAA TTTACGTCGC ATCTCACGCA GTCTCAGTGG CACCGTTGTC TCAGAGAGGG AGGAAGCTCC GGTTTCTTCC CACAGTTTTG ATTCATCAAA CGTGAGGAAG CCTTTGGAAA CCGGGCACCG TTGTTCCAGC TCCTCTTCCC TCCCTGTCAT CCATGACCCT TCTGTGTTTC TCCTCGGTCC CCAACTCTAC CTTCCCCAAC CACAGTTCCT GTCCCCAGAT GTCCTGATGC CCACCATGGC AGGGGAGCCC AATAGACTCC CAGGAACTTC AAGGAGTGTC CAGCAGTTTC TGGCTATGTG TGACAGGGGT GAAACTTCCC AAGGGGCCAA GTACACAGGA AGGACTTTGA ACTACCAGAG CCTCCCCCAT CGCTCCAGAA CAGACAACTC CTGGGCACCC TGGTCAGAGA CCAACCAGCA TATTGGGACC AGATTCCTGA CTACTCCAGG GTGCAATCCT CAACTAACCT ACACTGCCAC ACTACCAGAA AGAAGCAAGG GCCTTCAGGT TCCTCACACT CAGTCCTGGA GTGATCTTTT CCATTCACCC TCCCACCCTC CCATTGTTCA TCCTGTGTAC CCACCATCTA GCAGTCTTCA TGTACCCCTG AGGTCAGCTT GGAATTCAGA TCCTGTTCCA GGGTCCCGAA CCCCTGGTCC TCGAAGAGTA GATATGCCCC CAGATGATGA CTGGAGGCAA AGCAGTTATG CCTCCCACTC TGGACACAGG AGAACAGTGG GAGAGGGGTT TCTGTTTGTT CTATCAGATG CTCCCAGAAG AGAGCAGATC AGGGCTAGAG TCCTGCAGCA CAGTCAATGG GGATCCGGTA CCCTCGAGAC CGGTGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG TGACCGGTAA GTTTAAACCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGGCCAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGATTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT C
