pKD4-2×ME-pTrc-mCherry-Mob
$128.00
In stock
SKU
PL60522
100μg liquid plasmid(Instantly):$163
| name | pKD4-2×ME-pTrc-mCherry-Mob | another name | apKD4-2×ME-pTrc-mCherry-Mob |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | Red |
| Fragment species | Prokaryotic resistance | Amp,Kan | |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | TRC | Replicon | R6K |
| Competence | S17-1λpir | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | rrnBT1-F：GTTGTTTGTCGGTGAACGCTC | |
| 3′ sequencing primer | PKD-R：CAGTGACACAGGAACACTT | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATTGCAGC ATTACACGTC CTGTCTCTTA TACACATCTT TGAGCGATTG TGTAGGCTGG AGCTGCTTCG AAGTTCCTAT ACTTTCTAGA GAATAGGAAC TTCGGAATAG GAACTTCAAG ATCCCCTCAC GCTGCCGCAA GCACTCAGGG CGCAAGGGCT GCTAAAGGAA GCGGAACACG TAGAAAGCCA GTCCGCAGAA ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAGCTT CAAAAGCGCT CTGAAGTTCC TATACTTTCT AGAGAATAGG AACTTCGGAA TAGGAACTAA GGAGGATATT TTACTTGTAC AGCTCGTCCA TGCCGCCGGT GGAGTGGCGG CCCTCGGCGC GTTCGTACTG TTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC AACTTGATGT TGACGTTGTA GGCGCCGGGC AGCTGCACGG GCTTCTTGGC CTTGTAGGTG GTCTTGACCT CAGCGTCGTA GTGGCCGCCG TCCTTCAGCT TCAGCCTCTG CTTGATCTCG CCCTTCAGGG CGCCGTCCTC GGGGTACATC CGCTCGGAGG AGGCCTCCCA GCCCATGGTC TTCTTCTGCA TTACGGGGCC GTCGGAGGGG AAGTTGGTGC CGCGCAGCTT CACCTTGTAG ATGAACTCGC CGTCCTGCAG GGAGGAGTCC TGGGTCACGG TCACCACGCC GCCGTCCTCG AAGTTCATCA CGCGCTCCCA CTTGAAGCCC TCGGGGAAGG ACAGCTTCAA GTAGTCGGGG ATGTCGGCGG GGTGCTTCAC GTAGGCCTTG GAGCCGTACA TGAACTGAGG GGACAGGATG TCCCAGGCGA AGGGCAGGGG GCCACCCTTG GTCACCTTCA GCTTGGCGGT CTGGGTGCCC TCGTAGGGGC GGCCCTCGCC CTCGCCCTCG ATCTCGAACT CGTGGCCGTT CACGGAGCCC TCCATGTGCA CCTTGAAGCG CATGAACTCC TTGATGATGG CCATGTTATC CTCCTCGCCC TTGCTCACCA TGAATACTGT TTCCTGTGTG AAATATCCGC TCATGAGACA TTCCACATTG TTATCCGCTC ACAATTCCAC ACATTATACG AGCCGGATGA TTAATTGTCA ACATATGGAC CATAGATGTG TATAAGAGAC AGGGCTAATT CCCATGTCAG CCGTTAAGTG TTCCTGTGTC ACTGAAAATT GCTTTGAGAG GCTCTAAGGG CTTCTCAGTG CGTTACATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT AAAAGCTTTA AAAGCCTTAT ATATTCTTTT TTTTCTTATA AAACTTAAAA CCTTAGAGGC TATTTAAGTT GCTGATTTAT ATTAATTTTA TTGTTCAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTT AGCCATGAGA GCTTAGTACG TTAGCCATGA GGGTTTAGTT CGTTAAACAT GAGAGCTTAG TACGTTAAAC ATGAGAGCTT AGTACGTGAA ACATGAGAGC TTAGTACGTA CTATCAACAG GTTGAACTGC GGATCTTGCG GCCGCAAAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGC ATCGAGCCGA CCAGGCTTTC CACGCCCGCG TGCCGCTCCA TGTCGTTCGC GCGGTTCTCG GAAACGCGCT GCCGCGTTTC GTGATTGTCA CGCTCAAGCC CGTAGTCCCG TTCGAGCGTC GCGCAGAGGT CAGCGAGGGC GCGGTAGGCC CGATACGGCT CATGGATGGT GTTTCGGGTC GGGTGAATCT TGTTGATGGC GATATGGATG TGCAGGTTGT CGGTGTCGTG ATGCACGGCA CTGACGCGCT GATGCTCGGC GAAGCCAAGC CCAGCGCAGA TGCGGTCCTC AATCGCGCGC AACGTCTCCG CGTCGGGCTT CTCTCCCGCG CGGAAGCTAA CCAGCAGGTG ATAGGTCTTG TCGGCCTCGG AACGGGTGTT GCCGTGCTGG GTCGCCATCA CCTCGGCCAT GACAGCGGGC AGGGTGTTTG CCTCGCAGTT CGTGACGCGC ACGTGACCCA GGCGCTCGGT CTTGCCTTGC TCGTCGGTGA TGTACTTCAC CAGCTCCGCG AAGTCGCTCT TCTTGATGGA GCGCATGGGG ACGTGCTTGG CAATCACGCG CACCCCCCGG CCGTTTTAGC GGCTAAAAAA GTCATGGCTC TGCCCTCGGG CGGACCACGC CCATCATGAC CTTGCCAAGC TCGTCCTGCT TCTCTTCGAT CTTCGCCAGC AGGGCGAGGA TCGTGGCATC ACCGAACCGC GCCGTGCGCG GGTCGTCGGT GAGCCAGAGT TTCAGCAGGC CGCCCAGGCG GCCCAGGTCG CCATTGATGC GGGCCAGCTC GCGGACGTGC TCATAGTCCA CGACGCCCGT GATTTTGTAG CCCTGGCCGA CGGCCAGCAG GTAGGCCGAC AGGCTCATGC CGGCCGCCGC CGCCTTTTCC TCAATCGCTC TTCGTTCGTC TGGAAGGCAG TACACCTTGA TAGGTGGGCT GCCCTTCCTG GTTGGCTTGG TTTCATCAGC CATCCGCTTG CCCTCATCTG TTACGCCGGC GGTAGCCGGC CAGCCTCGCA GAGCAGGATT CCCGTTGAGC ACCGCCAGGT GCGAATAAGG GACAGTGAAG AAGGAACACC CGCTCGCGGG TGGGCCTACT TCACCTATCC TGCCCGGCTG ACGCCGTTGG ATACACCAAG GAAAGTCTAC ACGAACCCTT TGGCAAAATC CTGTATATCG TGCGAAAAAG GATGGATATA CCGAAAAAAT CGCTATAATG ACCCCGAAGC AGGGTTATGC AGCGGAAAAG CGCTGCTTCC CTGCTGTTTT GTGGAATATC TACCGACTGG AAACAGGCAA ATGCAGGAAA TTACTGAACT GAGGGGACAG GCGAGAGACG ATGCCAAAGA GCTACACCGA CGAGCTGGCC GAGTGGGTTG AATCCCGCGC GGCCAAGAAG CGCCGGCGTG ATGAGGCTGC GGTTGCGTTC CTGGCGGTGA GGGCGGATGT CGAGGCGGCG TTAGCGTCCG GCTATGCGCT CGTCACCATT TGGGAGCACA TGCGGGAAAC GGGGAAGGTC AAGTTCTCCT ACGAGACGTT CCGCTCGCAC GCCAGGCGGC ACATCAAGGC CAAGCCCGCC GATGTGCCCG CACCGCAGGC CAAGGCTGCG GAACCCGCGC CGGCACCCAA GACGCCGGAG CCACGGCGGC CGAAGCAGGG GGGCAAGGCT GAAAAGCCGG CCCCCGCTGC GGCCCCGACC GGCTTCACCT TCAACCCAAC ACCGGACAAA AAATGGCCCC CCGATGGTAG TGTGGGGTCT CCCCATGCGA GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC GTGGCCAGTG CCAAGCTTGC ATGC
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