pMLAAV-EnCMV-RfxCas13d
$113.00
In stock
SKU
PL60487
100μg liquid plasmid(Instantly):$134
| name | pMLAAV-EnCMV-RfxCas13d | another name | apMLAAV-EnCMV-RfxCas13d |
| Insert Size (bp) | Plasmid host | Mammary cell,Adeno-associated virus | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTGGAGC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGTCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCGT TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA CACCGGGACC GATCCAGCCT CCGCGGATTC GAATCCCGGC CGGGAACGGT GCATTGGAAC GCGGATTCCC CGTGCCAAGA GTGACGTAAG TACCGCCTAT AGAGTCTATA GGCCCACAAA AAATGCTTTC TTCTTTTAAT ATACTTTTTT GTTTATCTTA TTTCTAATAC TTTCCCTAAT CTCTTTCTTT CAGGGCAATA ATGATACAAT GTATCATGCC TCTTTGCACC ATTCTAAAGA ATAACAGTGA TAATTTCTGG GTTAAGGCAA TAGCAATATT TCTGCATATA AATATTTCTG CATATAAATT GTAACTGATG TAAGAGGTTT CATATTGCTA ATAGCAGCTA CAATCCAGCT ACCATTCTGC TTTTATTTTA TGGTTGGGAT AAGGCTGGAT TATTCTGAGT CCAAGCTAGG CCCTTTTGCT AATCATGTTC ATACCTCTTA TCTTCCTCCC ACAGCTCCTG GGCAACGTGC TGGTCTGTGT GCTGGCCCAT CACTTTGGCA AAGAATTGGG ATTCGAACAT CGATTGAATT GCCACCATGC CCAAGAAGAA GAGAAAGGTG GAGGCCAGCA TCGAAAAAAA AAAGTCCTTC GCCAAGGGCA TGGGCGTGAA GTCCACACTC GTGTCCGGCT CCAAAGTGTA CATGACAACC TTCGCCGAAG GCAGCGACGC CAGGCTGGAA AAGATCGTGG AGGGCGACAG CATCAGGAGC GTGAATGAGG GCGAGGCCTT CAGCGCTGAA ATGGCCGATA AAAACGCCGG CTATAAGATC GGCAACGCCA AATTCAGCCA TCCTAAGGGC TACGCCGTGG TGGCTAACAA CCCTCTGTAT ACAGGACCCG TCCAGCAGGA TATGCTCGGC CTGAAGGAAA CTCTGGAAAA GAGGTACTTC GGCGAGAGCG CTGATGGCAA TGACAATATT TGTATCCAGG TGATCCATAA CATCCTGGAC ATTGAAAAAA TCCTCGCCGA ATACATTACC AACGCCGCCT ACGCCGTCAA CAATATCTCC GGCCTGGATA AGGACATTAT TGGATTCGGC AAGTTCTCCA CAGTGTATAC CTACGACGAA TTCAAAGACC CCGAGCACCA TAGGGCCGCT TTCAACAATA ACGATAAGCT CATCAACGCC ATCAAGGCCC AGTATGACGA GTTCGACAAC TTCCTCGATA ACCCCAGACT CGGCTATTTC GGCCAGGCCT TTTTCAGCAA GGAGGGCAGA AATTACATCA TCAATTACGG CAACGAATGC TATGACATTC TGGCCCTCCT GAGCGGACTG AGGCACTGGG TGGTCCATAA CAACGAAGAA GAGTCCAGGA TCTCCAGGAC CTGGCTCTAC AACCTCGATA AGAACCTCGA CAACGAATAC ATCTCCACCC TCAACTACCT CTACGACAGG ATCACCAATG AGCTGACCAA CTCCTTCTCC AAGAACTCCG CCGCCAACGT GAACTATATT GCCGAAACTC TGGGAATCAA CCCTGCCGAA TTCGCCGAAC AATATTTCAG ATTCAGCATT ATGAAAGAGC AGAAAAACCT CGGATTCAAT ATCACCAAGC TCAGGGAAGT GATGCTGGAC AGGAAGGATA TGTCCGAGAT CAGGAAAAAT CATAAGGTGT TCGACTCCAT CAGGACCAAG GTCTACACCA TGATGGACTT TGTGATTTAT AGGTATTACA TCGAAGAGGA TGCCAAGGTG GCTGCCGCCA ATAAGTCCCT CCCCGATAAT GAGAAGTCCC TGAGCGAGAA GGATATCTTT GTGATTAACC TGAGGGGCTC CTTCAACGAC GACCAGAAGG ATGCCCTCTA CTACGATGAA GCTAATAGAA TTTGGAGAAA GCTCGAAAAT ATCATGCACA ACATCAAGGA ATTTAGGGGA AACAAGACAA GAGAGTATAA GAAGAAGGAC GCCCCTAGAC TGCCCAGAAT CCTGCCCGCT GGCCGTGATG TTTCCGCCTT CAGCAAACTC ATGTATGCCC TGACCATGTT CCTGGATGGC AAGGAGATCA ACGACCTCCT GACCACCCTG ATTAATAAAT TCGATAACAT CCAGAGCTTC CTGAAGGTGA TGCCTCTCAT CGGAGTCAAC GCTAAGTTCG TGGAGGAATA CGCCTTTTTC AAAGACTCCG CCAAGATCGC CGATGAGCTG AGGCTGATCA AGTCCTTCGC TAGAATGGGA GAACCTATTG CCGATGCCAG GAGGGCCATG TATATCGACG CCATCCGTAT TTTAGGAACC AACCTGTCCT ATGATGAGCT CAAGGCCCTC GCCGACACCT TTTCCCTGGA CGAGAACGGA AACAAGCTCA AGAAAGGCAA GCACGGCATG AGAAATTTCA TTATTAATAA CGTGATCAGC AATAAAAGGT TCCACTACCT GATCAGATAC GGTGATCCTG CCCACCTCCA TGAGATCGCC AAAAACGAGG CCGTGGTGAA GTTCGTGCTC GGCAGGATCG CTGACATCCA GAAAAAACAG GGCCAGAACG GCAAGAACCA GATCGACAGG TACTACGAAA CTTGTATCGG AAAGGATAAG GGCAAGAGCG TGAGCGAAAA GGTGGACGCT CTCACAAAGA TCATCACCGG AATGAACTAC GACCAATTCG ACAAGAAAAG GAGCGTCATT GAGGACACCG GCAGGGAAAA CGCCGAGAGG GAGAAGTTTA AAAAGATCAT CAGCCTGTAC CTCACCGTGA TCTACCACAT CCTCAAGAAT ATTGTCAATA TCAACGCCAG GTACGTCATC GGATTCCATT GCGTCGAGCG TGATGCTCAA CTGTACAAGG AGAAAGGCTA CGACATCAAT CTCAAGAAAC TGGAAGAGAA GGGATTCAGC TCCGTCACCA AGCTCTGCGC TGGCATTGAT GAAACTGCCC CCGATAAGAG AAAGGACGTG GAAAAGGAGA TGGCTGAAAG AGCCAAGGAG AGCATTGACA GCCTCGAGAG CGCCAACCCC AAGCTGTATG CCAATTACAT CAAATACAGC GACGAGAAGA AAGCCGAGGA GTTCACCAGG CAGATTAACA GGGAGAAGGC CAAAACCGCC CTGAACGCCT ACCTGAGGAA CACCAAGTGG AATGTGATCA TCAGGGAGGA CCTCCTGAGA ATTGACAACA AGACATGTAC CCTGTTCAGA AACAAGGCCG TCCACCTGGA AGTGGCCAGG TATGTCCACG CCTATATCAA CGACATTGCC GAGGTCAATT CCTACTTCCA ACTGTACCAT TACATCATGC AGAGAATTAT CATGAATGAG AGGTACGAGA AAAGCAGCGG AAAGGTGTCC GAGTACTTCG ACGCTGTGAA TGACGAGAAG AAGTACAACG ATAGGCTCCT GAAACTGCTG TGTGTGCCTT TCGGCTACTG TATCCCCAGG TTTAAGAACC TGAGCATCGA GGCCCTGTTC GATAGGAACG AGGCCGCCAA GTTCGACAAG GAGAAAAAGA AGGTGTCCGG CAATTCCTGA AGCTTGCCTC GAGCAGCGCT GCTCGAGAGA TCTACGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT GTAGGTAACC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA GGGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACG TCAAAGCAAC CATAGTACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGGC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTTATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGT
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