NM_004774.4;CRSP1; CRSP200; DRIP205; DRIP230; PBP
100μg liquid plasmid(Instantly):$134
| name | pEnCMV-MED1(human)-Linker-EGFP-SV40-Neo | another name | apEnCMV-MED1(human)-Linker-EGFP-SV40-Neo |
| Insert Size (bp) | 4743bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCC ACCATGAAAG CTCAGGGGGA AACCGAGGAG TCAGAAAAGC TGAGTAAGAT GAGTTCTCTC CTGGAACGGC TCCATGCAAA ATTTAACCAA AATAGACCCT GGAGTGAAAC CATTAAGCTT GTGCGTCAAG TCATGGAGAA GAGGGTTGTG ATGAGTTCTG GAGGGCATCA ACATTTGGTC AGCTGTTTGG AGACATTGCA GAAGGCTCTC AAAGTAACAT CTTTACCAGC AATGACTGAT CGTTTGGAGT CCATAGCAAG ACAGAATGGA CTGGGCTCTC ATCTCAGTGC CAGTGGCACT GAATGTTACA TCACGTCAGA TATGTTCTAT GTGGAAGTGC AGTTAGATCC TGCAGGACAG CTTTGTGATG TAAAAGTGGC TCACCATGGG GAGAATCCTG TGAGCTGTCC GGAGCTTGTA CAGCAGCTAA GGGAAAAAAA TTTTGATGAA TTTTCTAAGC ACCTTAAGGG CCTTGTTAAT CTGTATAACC TTCCAGGGGA CAACAAACTG AAGACTAAAA TGTACTTGGC TCTCCAATCC TTAGAACAAG ATCTTTCTAA AATGGCAATT ATGTACTGGA AAGCAACTAA TGCTGGTCCC TTGGATAAGA TTCTTCATGG AAGTGTTGGC TATCTCACAC CAAGGAGTGG GGGTCATTTA ATGAACCTGA AGTACTATGT CTCTCCTTCT GACCTACTGG ATGACAAGAC TGCATCTCCC ATCATTTTGC ATGAGAATAA TGTTTCTCGA TCTTTGGGCA TGAATGCATC AGTGACAATT GAAGGAACAT CTGCTGTGTA CAAACTCCCA ATTGCACCAT TAATTATGGG GTCACATCCA GTTGACAATA AATGGACCCC TTCCTTCTCC TCAATCACCA GTGCCAACAG TGTTGATCTT CCTGCCTGTT TCTTCTTGAA ATTTCCCCAG CCAATCCCAG TATCTAGAGC ATTTGTTCAG AAACTGCAGA ACTGCACAGG AATTCCATTG TTTGAAACTC AACCAACTTA TGCACCCCTG TATGAACTGA TCACTCAGTT TGAGCTATCA AAGGACCCTG ACCCCATACC TTTGAATCAC AACATGAGAT TTTATGCTGC TCTTCCTGGT CAGCAGCACT GCTATTTCCT CAACAAGGAT GCTCCTCTTC CAGATGGCCG AAGTCTACAG GGAACCCTTG TTAGCAAAAT CACCTTTCAG CACCCTGGCC GAGTTCCTCT TATCCTAAAT CTGATCAGAC ACCAAGTGGC CTATAACACC CTCATTGGAA GCTGTGTCAA AAGAACTATT CTGAAAGAAG ATTCTCCTGG GCTTCTCCAA TTTGAAGTGT GTCCTCTCTC AGAGTCTCGT TTCAGCGTAT CTTTTCAGCA CCCTGTGAAT GACTCCCTGG TGTGTGTGGT AATGGATGTG CAGGACTCAA CACATGTGAG CTGTAAACTC TACAAAGGGC TGTCGGATGC ACTGATCTGC ACAGATGACT TCATTGCCAA AGTTGTTCAA AGATGTATGT CCATCCCTGT GACGATGAGG GCTATTCGGA GGAAAGCTGA AACCATTCAA GCCGACACCC CAGCACTGTC CCTCATTGCA GAGACAGTTG AAGACATGGT GAAAAAGAAC CTGCCCCCGG CTAGCAGCCC AGGGTATGGC ATGACCACAG GCAACAACCC AATGAGTGGT ACCACTACAC CAACCAACAC CTTTCCGGGG GGTCCCATTA CCACCTTGTT TAATATGAGC ATGAGCATCA AAGATCGGCA TGAGTCGGTG GGCCATGGGG AGGACTTCAG CAAGGTGTCT CAGAACCCAA TTCTTACCAG TTTGTTGCAA ATCACAGGGA ACGGGGGGTC TACCATTGGC TCGAGTCCGA CCCCTCCTCA TCACACGCCG CCACCTGTCT CTTCGATGGC CGGCAACACC AAGAACCACC CGATGCTCAT GAACCTTCTT AAAGATAATC CTGCCCAGGA TTTCTCAACC CTTTATGGAA GCAGCCCTTT AGAAAGGCAG AACTCCTCTT CCGGCTCACC CCGCATGGAA ATATGCTCGG GGAGCAACAA GACCAAGAAA AAGAAGTCAT CAAGATTACC ACCTGAGAAA CCAAAGCACC AGACTGAAGA TGACTTTCAG AGGGAGCTAT TTTCAATGGA TGTTGACTCA CAGAACCCTA TCTTTGATGT CAACATGACA GCTGACACGC TGGATACGCC ACACATCACT CCAGCTCCAA GCCAGTGTAG CACTCCCCCA ACAACTTACC CACAACCAGT ACCTCACCCC CAACCCAGTA TTCAAAGGAT GGTCCGACTA TCCAGTTCAG ACAGCATTGG CCCAGATGTA ACTGACATCC TTTCAGACAT TGCAGAAGAA GCTTCTAAAC TTCCCAGCAC TAGTGATGAT TGCCCAGCCA TTGGCACCCC TCTTCGAGAT TCTTCAAGCT CTGGGCATTC TCAGAGTACC CTGTTTGACT CTGATGTCTT TCAAACTAAC AATAATGAAA ATCCATACAC TGATCCAGCT GATCTTATTG CAGATGCTGC TGGAAGCCCC AGTAGTGACT CTCCTACCAA TCATTTTTTT CATGATGGAG TAGATTTCAA TCCTGATTTA TTGAACAGCC AGAGCCAAAG TGGTTTTGGA GAAGAATATT TTGATGAAAG CAGCCAAAGT GGGGATAATG ATGATTTCAA AGGATTTGCA TCTCAGGCAC TAAATACTTT GGGGGTGCCA ATGCTTGGAG GTGATAATGG GGAGACCAAG TTTAAGGGCA ATAACCAAGC CGACACAGTT GATTTCAGTA TTATTTCAGT AGCCGGCAAA GCTTTAGCTC CTGCAGATCT TATGGAGCAT CACAGTGGTA GTCAGGGTCC TTTACTGACC ACTGGGGACT TAGGGAAAGA AAAGACTCAA AAGAGGGTAA AGGAAGGCAA TGGCACCAGT AATAGTACTC TCTCGGGGCC CGGATTAGAC AGCAAACCAG GGAAGCGCAG TCGGACCCCT TCTAATGATG GGAAAAGCAA AGATAAGCCT CCAAAGCGGA AGAAGGCAGA CACTGAGGGA AAGTCTCCAT CTCATAGTTC TTCTAACAGA CCTTTTACCC CACCTACCAG TACAGGTGGA TCTAAATCGC CAGGCAGTGC AGGAAGATCT CAGACTCCCC CAGGTGTTGC CACACCACCC ATTCCCAAAA TCACTATTCA GATTCCTAAG GGAACAGTGA TGGTGGGCAA GCCTTCCTCT CACAGTCAGT ATACCAGCAG TGGTTCTGTG TCTTCCTCAG GCAGCAAAAG CCACCATAGC CATTCTTCCT CCTCTTCCTC ATCTGCTTCC ACCTCAGGGA AGATGAAAAG CAGTAAATCA GAAGGTTCAT CAAGTTCCAA GTTAAGTAGC AGTATGTATT CTAGCCAGGG GTCTTCTGGA TCTAGCCAGT CCAAAAATTC ATCCCAGTCT GGGGGGAAGC CAGGCTCCTC TCCCATAACC AAGCATGGAC TGAGCAGTGG CTCTAGCAGC ACCAAGATGA AACCTCAAGG AAAGCCATCA TCACTTATGA ATCCTTCTTT AAGTAAACCA AACATATCCC CTTCTCATTC AAGGCCACCT GGAGGCTCTG ACAAGCTTGC CTCTCCAATG AAGCCTGTTC CTGGAACTCC TCCATCCTCT AAAGCCAAGT CCCCTATCAG TTCAGGTTCT GGTGGTTCTC ATATGTCTGG AACTAGTTCA AGCTCTGGCA TGAAGTCATC TTCAGGGTTA GGATCCTCAG GCTCGTTGTC CCAGAAAACT CCCCCATCAT CTAATTCCTG TACGGCATCT TCCTCCTCCT TTTCCTCAAG TGGCTCTTCC ATGTCATCCT CTCAGAACCA GCATGGGAGT TCTAAAGGAA AATCTCCCAG CAGAAACAAG AAGCCGTCCT TGACAGCTGT CATAGATAAA CTGAAGCATG GGGTTGTCAC CAGTGGCCCT GGGGGTGAAG ACCCACTGGA CGGCCAGATG GGGGTGAGCA CAAATTCTTC CAGCCATCCT ATGTCCTCCA AACATAACAT GTCAGGAGGA GAGTTTCAGG GCAAGCGTGA GAAAAGTGAT AAAGACAAAT CAAAGGTTTC CACCTCCGGG AGTTCAGTGG ATTCTTCTAA GAAGACCTCA GAGTCAAAAA ATGTGGGGAG CACAGGTGTG GCAAAAATTA TCATCAGTAA GCATGATGGA GGCTCCCCTA GCATTAAAGC CAAAGTGACT TTGCAGAAAC CTGGGGAAAG TAGTGGAGAA GGGCTTAGGC CTCAAATGGC TTCTTCTAAA AACTATGGCT CTCCACTCAT CAGTGGTTCC ACTCCAAAGC ATGAGCGTGG CTCTCCCAGC CATAGTAAGT CACCAGCATA TACCCCCCAG AATCTGGACA GTGAAAGTGA GTCAGGCTCC TCCATAGCAG AGAAATCTTA TCAGAATAGT CCCAGCTCAG ACGATGGTAT CCGACCACTT CCAGAATACA GCACAGAGAA ACATAAGAAG CACAAAAAGG AAAAGAAGAA AGTAAAAGAC AAAGATAGGG ACCGAGACCG GGACAAAGAC CGAGACAAGA AAAAATCTCA TAGCATCAAG CCAGAGAGTT GGTCCAAATC ACCCATCTCT TCAGACCAGT CCTTGTCTAT GACAAGTAAC ACAATCTTAT CTGCAGACAG ACCCTCAAGG CTCAGCCCAG ACTTTATGAT TGGGGAGGAA GATGATGATC TTATGGATGT GGCCCTGATT GGGAATGGAG GAGGGGGAAG CGATCCACCG GTCGCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTAAA GCGGCCGCGA CTCTAGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAGGCGTA AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTCCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG CGGCAGGCCC TGCCATAGCC TCAGGTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCAT GCAT
