pCMV6-LATS2(human)-FLAG
$99.00
In stock
SKU
PL60378
NM_014572.3;KPM
100μg liquid plasmid(Instantly):$120
| name | pCMV6-LATS2(human)-FLAG | another name | apCMV6-LATS2(human)-FLAG |
| Insert Size (bp) | 3264bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGCGA ATTCGCCACC ATGAGGCCAA AGACTTTTCC TGCCACGACT TATTCTGGAA ATAGCCGGCA GCGACTGCAA GAGATTCGTG AGGGGTTAAA ACAGCCATCC AAGTCTTCGG TTCAGGGGCT ACCCGCAGGA CCAAACAGTG ACACTTCCCT GGATGCCAAA GTCCTGGGGA GCAAAGATGC CACCAGGCAG CAGCAGCAGA TGAGAGCCAC CCCAAAGTTC GGACCTTATC AGAAAGCCTT GAGGGAAATC AGATATTCCT TGTTGCCTTT TGCTAATGAA TCGGGCACCT CTGCAGCTGC AGAAGTGAAC CGGCAAATGC TGCAGGAACT GGTGAACGCA GGATGCGACC AGGAGATGGC TGGCCGAGCT CTCAAGCAGA CTGGCAGCAG GAGCATCGAG GCCGCCCTGG AGTACATCAG CAAGATGGGC TACCTGGACC CGAGGAATGA GCAGATTGTG CGGGTCATTA AGCAGACCTC CCCAGGAAAG GGGCTCATGC CAACCCCAGT GACGCGGAGG CCCAGCTTCG AAGGAACCGG CGATTCGTTT GCGTCCTACC ACCAGCTGAG CGGTACCCCC TACGAGGGCC CAAGCTTCGG CGCTGACGGC CCCACGGCGC TGGAGGAGAT GCCGCGGCCG TACGTGGACT ACCTTTTCCC CGGAGTCGGC CCCCACGGGC CCGGCCACCA GCACCAGCAC CCACCCAAGG GCTACGGTGC CAGCGTAGAG GCAGCAGGGG CACACTTCCC GCTGCAGGGC GCGCACTACG GGCGGCCGCA CCTGCTGGTG CCTGGGGAAC CCCTGGGCTA CGGAGTGCAG CGCAGCCCCT CCTTCCAGAG CAAGACGCCG CCGGAGACCG GGGGTTACGC CAGCCTGCCC ACGAAGGGCC AGGGAGGACC GCCAGGCGCC GGCCTCGCTT TCCCACCCCC TGCCGCCGGG CTCTACGTGC CGCACCCACA CCACAAGCAG GCCGGTCCCG CGGCCCACCA GCTGCATGTG CTGGGCTCCC GCAGCCAGGT GTTCGCCAGC GACAGCCCCC CGCAGAGCCT GCTCACTCCC TCGCGGAACA GCCTCAACGT GGACCTGTAT GAATTGGGCA GCACCTCCGT CCAGCAGTGG CCGGCTGCCA CCCTGGCCCG CCGGGACTCC CTGCAGAAGC CGGGCCTGGA GGCGCCGCCG CGCGCGCACG TGGCCTTCCG GCCTGACTGC CCAGTGCCCA GCAGGACCAA CTCCTTCAAC AGCCACCAGC CGCGGCCCGG TCCGCCTGGC AAGGCCGAGC CCTCCCTGCC CGCCCCCAAC ACCGTGACGG CTGTCACGGC CGCGCACATC TTGCACCCGG TGAAGAGCGT GCGTGTGCTG AGGCCGGAGC CGCAGACGGC TGTGGGGCCC TCGCACCCCG CCTGGGTGCC CGCGCCTGCC CCGGCCCCCG CCCCCGCCCC CGCCCCGGCT GCGGAGGGCT TGGACGCCAA GGAGGAGCAT GCCCTGGCGC TGGGCGGCGC AGGCGCCTTC CCGCTGGACG TGGAGTACGG AGGCCCAGAC CGGAGGTGCC CGCCTCCGCC CTACCCGAAG CACCTGCTGC TGCGCAGCAA GTCGGAGCAG TACGACCTGG ACAGCCTGTG CGCAGGCATG GAGCAGAGCC TCCGTGCGGG CCCCAACGAG CCCGAGGGCG GCGACAAGAG CCGCAAAAGC GCCAAGGGGG ACAAAGGCGG AAAGGATAAA AAGCAGATTC AGACCTCTCC CGTTCCCGTC CGCAAAAACA GCAGAGACGA AGAGAAGAGA GAGTCACGCA TCAAGAGCTA CTCGCCATAC GCCTTTAAGT TCTTCATGGA GCAGCACGTG GAGAATGTCA TCAAAACCTA CCAGCAGAAG GTTAACCGGA GGCTGCAGCT GGAGCAAGAA ATGGCCAAAG CTGGACTCTG TGAAGCTGAG CAGGAGCAGA TGCGGAAGAT CCTCTACCAG AAAGAGTCTA ATTACAACAG GTTAAAGAGG GCCAAGATGG ACAAGTCTAT GTTTGTCAAG ATCAAAACCC TGGGGATCGG TGCCTTTGGA GAAGTGTGCC TTGCTTGTAA GGTGGACACT CACGCCCTGT ACGCCATGAA GACCCTAAGG AAAAAGGATG TCCTGAACCG GAATCAGGTG GCCCACGTCA AGGCCGAGAG GGACATCCTG GCCGAGGCAG ACAATGAGTG GGTGGTCAAA CTCTACTACT CCTTCCAAGA CAAAGACAGC CTGTACTTTG TGATGGACTA CATCCCTGGT GGGGACATGA TGAGCCTGCT GATCCGGATG GAGGTCTTCC CTGAGCACCT GGCCCGGTTC TACATCGCAG AGCTGACTTT GGCCATTGAG AGTGTCCACA AGATGGGCTT CATCCACCGA GACATCAAGC CTGATAACAT TTTGATAGAT CTGGATGGTC ACATTAAACT CACAGATTTC GGCCTCTGCA CTGGGTTCAG GTGGACTCAC AATTCCAAAT ATTACCAGAA AGGGAGCCAT GTCAGACAGG ACAGCATGGA GCCCAGCGAC CTCTGGGATG ATGTGTCTAA CTGTCGGTGT GGGGACAGGC TGAAGACCCT AGAGCAGAGG GCGCGGAAGC AGCACCAGAG GTGCCTGGCA CATTCACTGG TGGGGACTCC AAACTACATC GCACCCGAGG TGCTCCTCCG CAAAGGGTAC ACTCAACTCT GTGACTGGTG GAGTGTTGGA GTGATTCTCT TCGAGATGCT GGTGGGGCAG CCGCCCTTTT TGGCACCTAC TCCCACAGAA ACCCAGCTGA AGGTGATCAA CTGGGAGAAC ACGCTCCACA TTCCAGCCCA GGTGAAGCTG AGCCCTGAGG CCAGGGACCT CATCACCAAG CTGTGCTGCT CCGCAGACCA CCGCCTGGGG CGGAATGGGG CCGATGACCT GAAGGCCCAC CCCTTCTTCA GCGCCATTGA CTTCTCCAGT GACATCCGGA AGCAGCCAGC CCCCTACGTT CCCACCATCA GCCACCCCAT GGACACCTCG AATTTCGACC CCGTAGATGA AGAAAGCCCT TGGAACGATG CCAGCGAAGG TAGCACCAAG GCCTGGGACA CACTCACCTC GCCCAATAAC AAGCATCCTG AGCACGCATT TTACGAATTC ACCTTCCGAA GGTTCTTTGA TGACAATGGC TACCCCTTTC GATGCCCAAA GCCTTCAGGA GCAGAAGCTT CACAGGCTGA GAGCTCAGAT TTAGAAAGCT CTGATCTGGT GGATCAGACT GAAGGCTGCC AGCCTGTGTA CGTGGATTAC AAGGACGACG ATGACAAGTA GTCTAGATTG CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCTCGAGGA ACTGAAAAAC CAGAAAGTTA ATTCCCTATA GTGAGTCGTA TTAAATTCGT AATCATGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT T
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