pAAV-ZsGreen1-CD68(mouse)-shRNA1
$55.00
In stock
SKU
PL60164
100μg liquid plasmid(Instantly):$76
| name | pAAV-ZsGreen1-CD68(mouse)-shRNA1 | another name | apAAV-ZsGreen1-CD68(mouse)-shRNA1 |
| Insert Size (bp) | Plasmid host | Mammary cell,Adeno-associated virus | |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTGGAGC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGTCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGATT CGAATCCCGG CCGGGAACGG TGCATTGGAA CGCGGATTCC CCGTGCCAAG AGTGACGTAA GTACCGCCTA TAGAGTCTAT AGGCCCACAA AAAATGCTTT CTTCTTTTAA TATACTTTTT TGTTTATCTT ATTTCTAATA CTTTCCCTAA TCTCTTTCTT TCAGGGCAAT AATGATACAA TGTATCATGC CTCTTTGCAC CATTCTAAAG AATAACAGTG ATAATTTCTG GGTTAAGGCA ATAGCAATAT TTCTGCATAT AAATATTTCT GCATATAAAT TGTAACTGAT GTAAGAGGTT TCATATTGCT AATAGCAGCT ACAATCCAGC TACCATTCTG CTTTTATTTT ATGGTTGGGA TAAGGCTGGA TTATTCTGAG TCCAAGCTAG GCCCTTTTGC TAATCATGTT CATACCTCTT ATCTTCCTCC CACAGCTCCT GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC AAAGAATTGG GATTCGAACA TCGATTGAAT TCCACCATGG CCCAGTCCAA GCACGGCCTG ACCAAGGAGA TGACCATGAA GTACCGCATG GAGGGCTGCG TGGACGGCCA CAAGTTCGTG ATCACCGGCG AGGGCATCGG CTACCCCTTC AAGGGCAAGC AGGCCATCAA CCTGTGCGTG GTGGAGGGCG GCCCCTTGCC CTTCGCCGAG GACATCTTGT CCGCCGCCTT CATGTACGGC AACCGCGTGT TCACCGAGTA CCCCCAGGAC ATCGTCGACT ACTTCAAGAA CTCCTGCCCC GCCGGCTACA CCTGGGACCG CTCCTTCCTG TTCGAGGACG GCGCCGTGTG CATCTGCAAC GCCGACATCA CCGTGAGCGT GGAGGAGAAC TGCATGTACC ACGAGTCCAA GTTCTACGGC GTGAACTTCC CCGCCGACGG CCCCGTGATG AAGAAGATGA CCGACAACTG GGAGCCCTCC TGCGAGAAGA TCATCCCCGT GCCCAAGCAG GGCATCTTGA AGGGCGACGT GAGCATGTAC CTGCTGCTGA AGGACGGTGG CCGCTTGCGC TGCCAGTTCG ACACCGTGTA CAAGGCCAAG TCCGTGCCCC GCAAGATGCC CGACTGGCAC TTCATCCAGC ACAAGCTGAC CCGCGAGGAC CGCAGCGACG CCAAGAACCA GAAGTGGCAC CTGACCGAGC ACGCCATCGC CTCCGGCTCC GCCTTGCCCT AAAGATCTAC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTGTAGG TAACCGACCT GTTGGTGAGT AGATGCTTTC AGGGAAAGAA AGGAAGACAA GTTAAGTTAG TCCAAGGTGA TTCCCTCTCC AAAGGGCTGA CTGTGTGCCC TTTTCAGGAA CGTAGCAACT TGGAAGAAAA CCAGGCGTGC CTGAGGTCTC TGGTGGACAA GAAACTCTTG TTTCCTTTGC CCTCCTCTGG CTGCCTACAT GTATGCCTAC TTAATTAGGA TAGATTGTAT CCTCTGATGG CTATATTCTT TCCTGACCTA GGCCAGAGGC ATTGATGTGC AGCAGGTCTC CTTAGTCATC AACTATGACC TTCCCACCAA CAGGGAAAAC TACATCCACA GGTAAGTCTA GGTCTGACAG ACTAAACAAC CCCTATGCTG AAACCCAAGT GTTCCATCAT CCTTTTTACC CCCCTCAGAA TCGGTCGAGG TGGTCGGTTT GGTCGTAAGG GTGTGGCTAT TAACATGGTG ACCGAAGAAG ACAAGAGGAC TCTTCGAGAC ATTGAGACTT TCTACAACAC CTCCATTGAA GAGATGCCCC TCAACGTTGC TGACCTCATT TGAGGGGCTG TCCTGCGACC TGGCCCTAGC CCAGGGTTCA GTCCTGGGGT GGGGCTAAGG AAGAGCTGGA GGGGGGAGGG GAGGGAGCCA AGGGATGGAC ATCTTGTTTT TGTTTTGGCT TTTTTTTTTT TTGTTTCAGT TTTTTTTCTC TATGAATAAA TGTCACTTTT TGAGGCAAAA AGAAGGAACC GTGAACATTT TAGACACCCT TTTCTTTGGG GTAGGCTCTG CCCCAGGCGC CGTCTCCTTC CCCCCCCAAA CACTAATGCA TTTCCCTAAC CTAGTCACCT CGTTCCTAAA GGCTTTCCTA CCCCAGCCAA ATCTCCAAAA GTGAGTCAAG GGGCTAAAAA ACAAGGCTGG CCTCATTTGC TGGACCAAAT CTACAGGGAG AACCCCTGAG TGAGGTTGTC CAGGGAATTG TCCCCTGGTG AGGGGAGCAG GGGAGAGAAA ATGGTAGCCA TTTTTACATT GTTTTGTATA GTATTTATTG ATTCAGGAAA CAAACACAAA ATTCTGAATA AAATTACTTG GAAACTGCCT GTTTGGGCTT CTTGTTTCTT TCCCTTCCCT CCTACCTGCT AGTGTGGATC ACCTGCCCCC ATACTAGTTG ATTACTGAAT TTGCCATGTT GCTTCCTGCA ATACCAAATG ACCCACATTA CTAACATTTG GTAATTTGCC TCAGTGTTAG GCACAAGACA TGAGTGAAAC TTGCTGCATT AAGACTTAAA CTGGCTGGGT ATGGTGGCGC AAGCCTTTAA TCCCAGCATT CTGCCGGAGG CAGAAGCAGG TGGATTTCTG AGTTCGAGGC TAGCCTGGTC TACAAAAGTG AGTTCCAGGA CAGCCAAGGC TACACAAAAT ACCTGTCTTG AAAAACCAAA AAAAAAAAAT TCAACTGGTT GGTTAGGCCT AGCCCAGATG ATGTAGGTGT CAACTCACCT TGGGGATGTA GGGAGATGGT TGTTCTGTAG CTCTTCCTGT CCTGCAACTA AATAAATAGG CTAAGCTGAC CTCTTACATC TGCCTTCGGC CTCCTGTGCT AGGATTGGAG TGCATGGGTG GGGTTGCATT TCTTACCAAT GAGGAAAGGG CTTCCCATTT CTCTGCTGCA TTGTAAGCTC CCAAGTAGAG CAAGTTTTGC TTAGGTAAGT TCCGTGTGAG TCAGCTGCCT CATTCTCACG AGGTAACCAA GGCTTTGTAC CGCCCACTGA GAACGTCACT GCCAATCACA GCCTAATTGT GAAAACCAAT GGCTTGAGTG GGTTGCTAAA GCTGAGGTGT CTGAGTCAGG TTTGGGGTGG GATTATTTTA GTTAAGGGAA GTGAGGCTTT TCATTTCCTC TTCCAAGAGA AGGCAAAGGG GATTGGATTG AGGAAGGAAC TGGTGTAGCC TAGCTGGTCT GAGCATCTCT GCCGGATCCG CACTGTGTGT GTGTGCCACA CTCGAGTGTG GCACACACAC ACAGTGCTTT TTAAGCTTGC GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCTGC CTGCAGGGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGTCAAA GCAACCATAG TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGT
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