100μg liquid plasmid(Instantly):$134
| name | PiggyBac-EnCMV-RTP2(human)-IRES-REEP1(human)-P2A-EGFP-Neo(1synonymous mutations) | another name | aPiggyBac-EnCMV-RTP2(human)-IRES-REEP1(human)-P2A-EGFP-Neo(1synonymous mutations) |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | pEGFP-N-3'：CGTCGCCGTCCAGCTCGACCAG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA ATACGACTCA CTATAGGGAG ACCCAAGCTT GCCACCATGT GTACCAGCTT GACCACTTGT GAGTGGAAGA AAGTCTTCTA TGAGAAGATG GAGGTGGCAA AGCCAGCGGA CAGCTGGGAG CTCATCATAG ACCCCAACCT CAAGCCCAGT GAGCTGGCCC CTGGCTGGAA GCAGTACCTG GAGCAGCACG CCTCAGGCAG GTTCCACTGC TCCTGGTGCT GGCACACCTG GCAGTCTGCC CATGTGGTCA TCCTCTTCCA CATGTTCCTG GACCGCGCCC AGCGGGCGGG CTCGGTGCGC ATGCGCGTCT TCAAGCAGCT GTGCTATGAG TGCGGCACGG CGCGGCTGGA CGAGTCCAGC ATGCTGGAGG AGAACATCGA GGGCCTGGTG GACAACCTCA TCACCAGCCT GCGCGAGCAG TGCTACGAGG AGGATGGTGG CCAGTACCGC ATCCACGTGG CCAGCCGCCC GGACAGCGGG CCGCATCGTG CAGAGTTCTG TGAGGCCTGC CAGGAGGGCA TCGTTCACTG GAAGCCCAGC GAGAAGCTGC TGGAGGAGGA GGTGACCACC TACACCTCTG AAGCCTCCAA GCCGAGGGCC CAGGCGGGAT CCGGCTACAA CTTCTTGTCT CTTCGCTGGT GCCTCTTCTG GGCCTCTCTC TGCCTGCTCG TTGTTTACCT GCAGTTCTCC TTCCTCAGTC CTGCCTTCTT TTAGCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG GAATAAGGCC GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTGGC AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA AGCGTATTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGATCTGAT CTGGGGCCTC GGTGCACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA ACGTCTAGGC CCCCCGAACC ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAAGC CACCATGGTG TCATGGATCA TCTCCAGGCT GGTGGTGCTT ATATTTGGCA CCCTTTACCC TGCGTATTAT TCCTACAAGG CTGTGAAATC AAAGGACATT AAGGAATATG TCAAATGGAT GATGTACTGG ATTATATTTG CACTTTTCAC CACAGCAGAG ACATTCACAG ACATCTTCCT TTGTTGGTTT CCATTCTATT ATGAACTAAA AATAGCATTT GTAGCCTGGC TGCTGTCTCC CTACACAAAA GGCTCCAGCC TCCTGTACAG GAAGTTTGTA CATCCCACGC TATCTTCAAA AGAAAAGGAA ATCGATGATT GTCTGGTCCA AGCAAAAGAC CGAAGTTACG ATGCCCTTGT GCACTTCGGG AAGCGGGGCT TGAACGTGGC CGCCACAGCG GCTGTGATGG CTGCTTCCAA GGGACAGGGT GCCTTATCGG AGAGACTGCG GAGCTTCAGC ATGCAGGACC TCACCACCAT CAGGGGAGAC GGCGCCCCTG CTCCCTCGGG CCCCCCACCA CCGGGGTCTG GGCGGGCCAG CGGCAAACAC GGCCAGCCTA AGATGTCCAG GAGTGCTTCT GAGAGCGCTA GCAGCTCAGT GTGTACCTGC TGCTCCACCT GCAGGACCTG GAAAGTGGTT GAGGGAGATG TGAATGAGGG TGGTATGAAG GCATGGGAGC CCCACCAGGT CCAAAACCCA TTGGCGTTTT CTGACGACGA GGAGGAAGAC CTGCTGGATT TCATGTACAA GTATAAAGCA CCTCGAAGGA TGGAATTGCC CCTGGAGGCA CCGCCTAGAA TCCTTCGATC TCGCTTCAGG AAGAAAAGTA CCTCATCCTC GGCCACCGAA ACCACGGCCA CGAACTTCTC TCTGTTAAAG CAAGCAGGAG ACGTGGAAGA AAACCCCGGT CCTCCGGTCG CCACCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGCGACTCT AGAGGGCCCT ATTCTATAGT GTCACCTAAA TGCTAGAGCT CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTGTCGAC GAAGTTCCTA TTCCGAAGTT CCTATTCTCT AGAAAGTATA GGAACTTCCT CGAGTTAATT AACGAGAGCA TAATATTGAT ATGTGCCAAA GTTGTTTCTG ACTGACTAAT AAGTATAATT TGTTTCTATT ATGTATAGGT TAAGCTAATT ACTTATTTTA TAATACAACA TGACTGTTTT TAAAGTACAA AATAAGTTTA TTTTTGTAAA AGAGAGAATG TTTAAAAGTT TTGTTACTTT ATAGAAGAAA TTTTGAGTTT TTGTTTTTTT TTAATAAATA AATAAACATA AATAAATTGT TTGTTGAATT TATTATTAGT ATGTAAGTGT AAATATAATA AAACTTAATA TCTATTCAAA TTAATAAATA AACCTCGATA TACAGACCGA TAAAACACAT GCGTCAATTT TACGCATGAT TATCTTTAAC GTACGTCACA ATATGATTAT CTTTCTAGGG TTAAATAATA GTTTCTAATT TTTTTATTAT TCAGCCTGCT GTCGTGAATA CCGAGCTCCA ATTCGCCCTA TAGTGAGTCG TATTACAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGCTTAC AATTTAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT ACCTCGCGCG ACTTGGTTTG CCATTCTTTA GCGCGCGTCG CGTCACACAG CTTGGCCACA ATGTGGTTTT TGTCAAACGA AGATTCTATG ACGTGTTTAA AGTTTAGGTC GAGTAAAGCG CAAATCTTTT TTAACCCTAG AAAGATAGTC TGCGTAAAAT TGACGCATGC ATTCTTGAAA TATTGCTCTC TCTTTCTAAA TAGCGCGAAT CCGTCGCTGT GCATTTAGGA CATCTCAGTC GCCGCTTGGA GCTCCCGTGA GGCGTGCTTG TCAATGCGGT AAGTGTCACT GATTTTGAAC TATAACGACC GCGTGAGTCA AAATGACGCA TGATTATCTT TTACGTGACT TTTAAGATTT AACTCATACG ATAATTATAT TGTTATTTCA TGTTCTACTT ACGTGATAAC TTATTATATA TATATTTTCT TGTTATAGAT ATCGAAGTTC CTATTCCGAA GTTCCTATTC TTCAAATAGT ATAGGAACTT CGTCGAGATC GATGGCGCGC CATAACTTCG TATAGCATAC ATTATACGAA GTTATGAATT TCGAGCAGTG TGGTTTTCAA GAGGAAGCAA AAAGCCTCTC CACCCAGGCC TGGAATGTTT CCACCCAATG TCGAGCAGTG TGGTTTTGCA AGAGGAAGCA AAAAGCCTCT CCACCCAGGC CTGGAATGTT TCCACCCAAT GTCGAGCAAA CCCCGCCCAG CGTCTTGTCA TTGGCGAATT CGAACACGCA GATGCAGTCG GGGCGGCGCG GTCCCAGGTC CACTTCGCAT ATTAAGGTGA CGCGTGTGGC CTCGAACACC GAGCGACCCT GCAGCCAATA TGGGATCGGC CATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGATGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGGGGATC GGCAATAAAA AGACAGAATA AAACGCACGG GTGTTGGGTC GTTTGTTCGG ATCAATTCAT AACTTCGTAT AGCATACATT ATACGAAGTT ATGGCGCGCC TTAATTAAGG ATCCGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT T
