pKD46-Tet-Cas9-SacB
$113.00
In stock
SKU
PL60082
100μg liquid plasmid(Instantly):$149
| name | pKD46-Tet-Cas9-SacB | another name | apKD46-Tet-Cas9-SacB |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | CRISPR,Red |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | araBAD | Replicon | pSC101 |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | PBAD30.F：AGATTAGCGGATCCTACCTG | |
| 3′ sequencing primer | pKD46-R：TCTAGCAGAATTTACAGATACCC | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATCGATTTA TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC GGAACTCGCT CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT CGTCAAAACC AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA GCTTCGCCTG GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT GGCGGAAAAG ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA TATCAAAATT GCTGTCTGCC AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT TATCCATCGG TGGATGGAGC GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT CAAGCAGATT TATCGCCAGC AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA TTTGCCCAAA CAGGTCGCTG AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG TATTGGCAAA TATTGACGGC CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT AAACCCACTG GTGATACCAT TCGCGAGCCT CCGGATGACG ACCGTAGTGA TGAATCTCTC CTGGCGGGAA CAGCAAAATA TCACCCGGTC GGCAAACAAA TTCTCGTCCC TGATTTTTCA CCACCCCCTG ACCGCGAATG GTGAGATTGA GAATATAACC TTTCATTCCC AGCGGTCGGT CGATAAAAAA ATCGAGATAA CCGTTGGCCT CAATCGGCGT TAAACCCGCC ACCAGATGGG CATTAAACGA GTATCCCGGC AGCAGGGGAT CATTTTGCGC TTCAGCCATA CTTTTCATAC TCCCGCCATT CAGAGAAGAA ACCAATTGTC CATATTGCAT CAGACATTGC CGTCACTGCG TCTTTTACTG GCTCTTCTCG CTAACCAAAC CGGTAACCCC GCTTATTAAA AGCATTCTGT AACAAAGCGG GACCAAAGCC ATGACAAAAA CGCGTAACAA AAGTGTCTAT AATCACGGCA GAAAAGTCCA CATTGATTAT TTGCACGGCG TCACACTTTG CTATGCCATA GCATTTTTAT CCATAAGATT AGCGGATCCT ACCTGACGCT TTTTATCGCA ACTCTCTACT GTTTCTCCAT ACCCGTTTTT TTGGGAATTC GAGCTCTAAG GAGGTTATAA AAAATGGATA TTAATACTGA AACTGAGATC AAGCAAAAGC ATTCACTAAC CCCCTTTCCT GTTTTCCTAA TCAGCCCGGC ATTTCGCGGG CGATATTTTC ACAGCTATTT CAGGAGTTCA GCCATGAACG CTTATTACAT TCAGGATCGT CTTGAGGCTC AGAGCTGGGC GCGTCACTAC CAGCAGCTCG CCCGTGAAGA GAAAGAGGCA GAACTGGCAG ACGACATGGA AAAAGGCCTG CCCCAGCACC TGTTTGAATC GCTATGCATC GATCATTTGC AACGCCACGG GGCCAGCAAA AAATCCATTA CCCGTGCGTT TGATGACGAT GTTGAGTTTC AGGAGCGCAT GGCAGAACAC ATCCGGTACA TGGTTGAAAC CATTGCTCAC CACCAGGTTG ATATTGATTC AGAGGTATAA AACGAATGAG TACTGCACTC GCAACGCTGG CTGGGAAGCT GGCTGAACGT GTCGGCATGG ATTCTGTCGA CCCACAGGAA CTGATCACCA CTCTTCGCCA GACGGCATTT AAAGGTGATG CCAGCGATGC GCAGTTCATC GCATTACTGA TCGTTGCCAA CCAGTACGGC CTTAATCCGT GGACGAAAGA AATTTACGCC TTTCCTGATA AGCAGAATGG CATCGTTCCG GTGGTGGGCG TTGATGGCTG GTCCCGCATC ATCAATGAAA ACCAGCAGTT TGATGGCATG GACTTTGAGC AGGACAATGA ATCCTGTACA TGCCGGATTT ACCGCAAGGA CCGTAATCAT CCGATCTGCG TTACCGAATG GATGGATGAA TGCCGCCGCG AACCATTCAA AACTCGCGAA GGCAGAGAAA TCACGGGGCC GTGGCAGTCG CATCCCAAAC GGATGTTACG TCATAAAGCC ATGATTCAGT GTGCCCGTCT GGCCTTCGGA TTTGCTGGTA TCTATGACAA GGATGAAGCC GAGCGCATTG TCGAAAATAC TGCATACACT GCAGAACGTC AGCCGGAACG CGACATCACT CCGGTTAACG ATGAAACCAT GCAGGAGATT AACACTCTGC TGATCGCCCT GGATAAAACA TGGGATGACG ACTTATTGCC GCTCTGTTCC CAGATATTTC GCCGCGACAT TCGTGCATCG TCAGAACTGA CACAGGCCGA AGCAGTAAAA GCTCTTGGAT TCCTGAAACA GAAAGCCGCA GAGCAGAAGG TGGCAGCATG ACACCGGACA TTATCCTGCA GCGTACCGGG ATCGATGTGA GAGCTGTCGA ACAGGGGGAT GATGCGTGGC ACAAATTACG GCTCGGCGTC ATCACCGCTT CAGAAGTTCA CAACGTGATA GCAAAACCCC GCTCCGGAAA GAAGTGGCCT GACATGAAAA TGTCCTACTT CCACACCCTG CTTGCTGAGG TTTGCACCGG TGTGGCTCCG GAAGTTAACG CTAAAGCACT GGCCTGGGGA AAACAGTACG AGAACGACGC CAGAACCCTG TTTGAATTCA CTTCCGGCGT GAATGTTACT GAATCCCCGA TCATCTATCG CGACGAAAGT ATGCGTACCG CCTGCTCTCC CGATGGTTTA TGCAGTGACG GCAACGGCCT TGAACTGAAA TGCCCGTTTA CCTCCCGGGA TTTCATGAAG TTCCGGCTCG GTGGTTTCGA GGCCATAAAG TCAGCTTACA TGGCCCAGGT GCAGTACAGC ATGTGGGTGA CGCGAAAAAA TGCCTGGTAC TTTGCCAACT ATGACCCGCG TATGAAGCGT GAAGGCCTGC ATTATGTCGT GATTGAGCGG GATGAAAAGT ACATGGCGAG TTTTGACGAG ATCGTGCCGG AGTTCATCGA AAAAATGGAC GAGGCACTGG CTGAAATTGG TTTTGTATTT GGGGAGCAAT GGCGATGACG CATCCTCACG ATAATATCCG GGTAGGCGCA ATCACTTTCG TCTACTCCGT TACAAAGCGA GGCTGGGTAT TTCCCGGCCT TTCTGTTATC CGAAATCCAC TGAAAGCACA GCGGCTGGCT GAGGAGATAA ATAATAAACG AGGGGCTGTA TGCACAAAGC ATCTTCTGTT GAGTTAAGAA CGAGTATCGA GATGGCACAT AGCCTTGCTC AAATTGGAAT CAGGTTTGTG CCAATACCAG TAGAAACAGA CGAAGAATCT TATTTGTTAA CTGTTAATTG TCCTTGTTCA AGGATGCTGT CTTTGACAAC AGATGTTTTC TTGCCTTTGA TGTTCAGCAG GAAGCTCGGC GCAAACGTTG ATTGTTTGTC TGCGTAGAAT CCTCTGTTTG TCATATAGCT TGTAATCACG ACATTGTTTC CTTTCGCTTG AGGTACAGCG AAGTGTGAGT AAGTAAAGGT TACATCGTTA GGATCAAGAT CCATTTTTAA CACAAGGCCA GTTTTGTTCA GCGGCTTGTA TGGGCCAGTT AAAGAATTAG AAACATAACC AAGCATGTAA ATATCGTTAG ACGTAATGCC GTCAATCGTC ATTTTTGATC CGCGGGAGTC AGTGAACAGG TACCATTTGC CGTTCATTTT AAAGACGTTC GCGCGTTCAA TTTCATCTGT TACTGTGTTA GATGCAATCA GCGGTTTCAT CACTTTTTTC AGTGTGTAAT CATCGTTTAG CTCAATCATA CCGAGAGCGC CGTTTGCTAA CTCAGCCGTG CGTTTTTTAT CGCTTTGCAG AAGTTTTTGA CTTTCTTGAC GGAAGAATGA TGTGCTTTTG CCATAGTATG CTTTGTTAAA TAAAGATTCT TCGCCTTGGT AGCCATCTTC AGTTCCAGTG TTTGCTTCAA ATACTAAGTA TTTGTGGCCT TTATCTTCTA CGTAGTGAGG ATCTCTCAGC GTATGGTTGT CGCCTGAGCT GTAGTTGCCT TCATCGATGA ACTGCTGTAC ATTTTGATAC GTTTTTCCGT CACCGTCAAA GATTGATTTA TAATCCTCTA CACCGTTGAT GTTCAAAGAG CTGTCTGATG CTGATACGTT AACTTGTGCA GTTGTCAGTG TTTGTTTGCC GTAATGTTTA CCGGAGAAAT CAGTGTAGAA TAAACGGATT TTTCCGTCAG ATGTAAATGT GGCTGAACCT GACCATTCTT GTGTTTGGTC TTTTAGGATA GAATCATTTG CATCGAATTT GTCGCTGTCT TTAAAGACGC GGCCAGCGTT TTTCCAGCTG TCAATAGAAG TTTCGCCGAC TTTTTGATAG AACATGTAAA TCGATGTGTC ATCCGCATTT TTAGGATCTC CGGCTAATGC AAAGACGATG TGGTAGCCGT GATAGTTTGC GACAGTGCCG TCAGCGTTTT GTAATGGCCA GCTGTCCCAA ACGTCCAGGC CTTTTGCAGA AGAGATATTT TTAATTGTGG ACGAATCAAA TTCAGAAACT TGATATTTTT CATTTTTTTG CTGTTCAGGG ATTTGCAGCA TATCATGGCG TGTAATATGG GAAATGCCGT ATGTTTCCTT ATATGGCTTT TGGTTCGTTT CTTTCGCAAA CGCTTGAGTT GCGCCTCCTG CCAGCAGTGC GGTAGTAAAG GTTAATACTG TTGCTTGTTT TGCAAACTTT TTGATGTTCA TCGTTGACAC TCTATCGTTG ATAGAGTTAT TTTACCACTC CCTATCAGTG ATAGAGAAAA GAATTCAAAA GATCTAAAGA GGAGAAAGGA TCTATGGATA AGAAATACTC AATAGGCTTA GATATCGGCA CAAATAGCGT CGGATGGGCG GTGATCACTG ATGAATATAA GGTTCCGTCT AAAAAGTTCA AGGTTCTGGG AAATACAGAC CGCCACAGTA TCAAAAAAAA TCTTATAGGG GCTCTTTTAT TTGACAGTGG AGAGACAGCG GAAGCGACTC GTCTCAAACG GACAGCTCGT AGAAGGTATA CACGTCGGAA GAATCGTATT TGTTATCTAC AGGAGATTTT TTCAAATGAG ATGGCGAAAG TAGATGATAG TTTCTTTCAT CGACTTGAAG AGTCTTTTTT GGTGGAAGAA GACAAGAAGC ATGAACGTCA TCCTATTTTT GGAAATATAG TAGATGAAGT TGCTTATCAT GAGAAATATC CAACTATCTA TCATCTGCGA AAAAAATTGG TAGATTCTAC TGATAAAGCG GATTTGCGCT TAATCTATTT GGCCTTAGCG CATATGATTA AGTTTCGTGG TCATTTTTTG ATTGAGGGAG ATTTAAATCC TGATAATAGT GATGTGGACA AACTATTTAT CCAGTTGGTA CAAACCTACA ATCAATTATT TGAAGAAAAC CCTATTAACG CAAGTGGAGT AGATGCTAAA GCGATTCTTT CTGCACGATT GAGTAAATCA AGACGATTAG AAAATCTCAT TGCTCAGCTC CCCGGTGAGA AGAAAAATGG CTTATTTGGG AATCTCATTG CTTTGTCATT GGGTTTGACC CCTAATTTTA AATCAAATTT TGATTTGGCA GAAGATGCTA AATTACAGCT TTCAAAAGAT ACTTACGATG ATGATTTAGA TAATTTATTG GCGCAAATTG GAGATCAATA TGCTGATTTG TTTTTGGCAG CTAAGAATTT ATCAGATGCT ATTTTACTTT CAGATATCCT AAGAGTAAAT ACTGAAATAA CTAAGGCTCC CCTATCAGCT TCAATGATTA AACGCTACGA TGAACATCAT CAAGACTTGA CTCTTTTAAA AGCTTTAGTT CGACAACAAC TTCCAGAAAA GTATAAAGAA ATCTTTTTTG ATCAATCAAA AAACGGATAT GCAGGTTATA TTGATGGGGG AGCTAGCCAA GAAGAATTTT ATAAATTTAT CAAACCAATT TTAGAAAAAA TGGATGGTAC TGAGGAATTA TTGGTGAAAC TAAATCGTGA AGATTTGCTG CGCAAGCAAC GGACCTTTGA CAACGGCTCT ATTCCCCATC AAATTCACTT GGGTGAGCTG CATGCTATTT TGAGAAGACA AGAAGACTTT TATCCATTTT TAAAAGACAA TCGTGAGAAG ATTGAAAAAA TCTTGACTTT TCGAATTCCT TATTATGTTG GTCCATTGGC GCGTGGCAAT AGTCGTTTTG CATGGATGAC TCGGAAGTCT GAAGAAACAA TTACCCCATG GAATTTTGAA GAAGTTGTCG ATAAAGGTGC TTCAGCTCAA TCATTTATTG AACGCATGAC AAACTTTGAT AAAAATCTTC CAAATGAAAA AGTACTACCA AAACATAGTT TGCTTTATGA GTATTTTACG GTTTATAACG AATTGACAAA GGTCAAATAT GTTACTGAAG GAATGCGAAA ACCAGCATTT CTTTCAGGTG AACAGAAGAA AGCCATTGTT GATTTACTCT TCAAAACAAA TCGAAAAGTA ACCGTTAAGC AATTAAAAGA AGATTATTTC AAAAAAATAG AATGTTTTGA TAGTGTTGAA ATTTCAGGAG TTGAAGATAG ATTTAATGCT TCATTAGGTA CCTACCATGA TTTGCTAAAA ATTATTAAAG ATAAAGATTT TTTGGATAAT GAAGAAAATG AAGATATCTT AGAGGATATT GTTTTAACAT TGACCTTATT TGAAGATAGG GAGATGATTG AGGAAAGACT TAAAACATAT GCTCACCTCT TTGATGATAA GGTGATGAAA CAGCTTAAAC GTCGCCGTTA TACTGGTTGG GGACGTTTGT CTCGAAAATT GATTAATGGT ATTAGGGATA AGCAATCTGG CAAAACAATA TTAGATTTTT TGAAATCAGA TGGTTTTGCC AATCGCAATT TTATGCAGCT GATCCATGAT GATAGTTTGA CATTTAAAGA AGACATTCAA AAAGCACAAG TGTCTGGACA AGGCGATAGT TTACATGAAC ATATTGCAAA TTTAGCTGGT AGCCCTGCTA TTAAAAAAGG TATTTTACAG ACTGTAAAAG TTGTTGATGA ATTGGTCAAA GTAATGGGGC GGCATAAGCC AGAAAATATC GTTATTGAAA TGGCACGTGA AAATCAGACA ACTCAAAAGG GCCAGAAAAA TTCGCGAGAG CGTATGAAAC GAATCGAAGA AGGTATCAAA GAATTAGGAA GTCAGATTCT TAAAGAGCAT CCTGTTGAAA ATACTCAATT GCAAAATGAA AAGCTCTATC TCTATTATCT CCAAAATGGA AGAGACATGT ATGTGGACCA AGAATTAGAT ATTAATCGTT TAAGTGATTA TGATGTCGAT CACATTGTTC CACAAAGTTT CCTTAAAGAC GATTCAATAG ACAATAAGGT CTTAACGCGT TCTGATAAAA ATCGTGGTAA ATCGGATAAC GTTCCAAGTG AAGAAGTAGT CAAAAAGATG AAAAACTATT GGAGACAACT TCTAAACGCC AAGTTAATCA CTCAACGTAA GTTTGATAAT TTAACGAAAG CTGAACGTGG AGGTTTGAGT GAACTTGATA AAGCTGGTTT TATCAAACGC CAATTGGTTG AAACTCGCCA AATCACTAAG CATGTGGCAC AAATTTTGGA TAGTCGCATG AATACTAAAT ACGATGAAAA TGATAAACTT ATTCGAGAGG TTAAAGTGAT TACCTTAAAA TCTAAATTAG TTTCTGACTT CCGAAAAGAT TTCCAATTCT ATAAAGTACG TGAGATTAAC AATTACCATC ATGCCCATGA TGCGTATCTA AATGCCGTCG TTGGAACTGC TTTGATTAAG AAATATCCAA AACTTGAATC GGAGTTTGTC TATGGTGATT ATAAAGTTTA TGATGTTCGT AAAATGATTG CTAAGTCTGA GCAAGAAATA GGCAAAGCAA CCGCAAAATA TTTCTTTTAC TCTAATATCA TGAACTTCTT CAAAACAGAA ATTACACTTG CAAATGGAGA GATTCGCAAA CGCCCTCTAA TCGAAACTAA TGGGGAAACT GGAGAAATTG TCTGGGATAA AGGGCGAGAT TTTGCCACAG TGCGCAAAGT ATTGTCCATG CCCCAAGTCA ATATTGTCAA GAAAACAGAA GTACAGACAG GCGGATTCTC CAAGGAGTCA ATTTTACCAA AAAGAAATTC GGACAAGCTT ATTGCTCGTA AAAAAGACTG GGATCCAAAA AAATATGGTG GTTTTGATAG TCCAACGGTA GCTTATTCAG TCCTAGTGGT TGCTAAGGTG GAAAAAGGGA AATCGAAGAA GTTAAAATCC GTTAAAGAGT TACTAGGGAT CACAATTATG GAAAGAAGTT CCTTTGAAAA AAATCCGATT GACTTTTTAG AAGCTAAAGG ATATAAGGAA GTTAAAAAAG ACTTAATCAT TAAACTACCT AAATATAGTC TTTTTGAGTT AGAAAACGGT CGTAAACGGA TGCTGGCTAG TGCCGGAGAA TTACAAAAAG GAAATGAGCT GGCTCTGCCA AGCAAATATG TGAATTTTTT ATATTTAGCT AGTCATTATG AAAAGTTGAA GGGTAGTCCA GAAGATAACG AACAAAAACA ATTGTTTGTG GAGCAGCATA AGCATTATTT AGATGAGATT ATTGAGCAAA TCAGTGAATT TTCTAAGCGT GTTATTTTAG CAGATGCCAA TTTAGATAAA GTTCTTAGTG CATATAACAA ACATAGAGAC AAACCAATAC GTGAACAAGC AGAAAATATT ATTCATTTAT TTACGTTGAC GAATCTTGGA GCTCCCGCTG CTTTTAAATA TTTTGATACA ACAATTGATC GTAAACGATA TACGTCTACA AAAGAAGTTT TAGATGCCAC TCTTATCCAT CAATCCATCA CTGGTCTTTA TGAAACACGC ATTGATTTGA GTCAGCTAGG AGGTGACTAA CTCGAGTAAG GATCTCCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA GAGTCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGCA GGTATGGACA GTTTTCCCTT TGATATGTAA CGGTGAACAG TTGTTCTACT TTTGTTTGTT AGTCTTGATG CTTCACTGAT AGATACAAGA GCCATAAGAA CCTCAGATCC TTCCGTATTT AGCCAGTATG TTCTCTAGTG TGGTTCGTTG TTTTTGCGTG AGCCATGAGA ACGAACCATT GAGATCATAC TTACTTTGCA TGTCACTCAA AAATTTTGCC TCAAAACTGG TGAGCTGAAT TTTTGCAGTT AAAGCATCGT GTAGTGTTTT TCTTAGTCCG TTACGTAGGT AGGAATCTGA TGTAATGGTT GTTGGTATTT TGTCACCATT CATTTTTATC TGGTTGTTCT CAAGTTCGGT TACGAGATCC ATTTGTCTAT CTAGTTCAAC TTGGAAAATC AACGTATCAG TCGGGCGGCC TCGCTTATCA ACCACCAATT TCATATTGCT GTAAGTGTTT AAATCTTTAC TTATTGGTTT CAAAACCCAT TGGTTAAGCC TTTTAAACTC ATGGTAGTTA TTTTCAAGCA TTAACATGAA CTTAAATTCA TCAAGGCTAA TCTCTATATT TGCCTTGTGA GTTTTCTTTT GTGTTAGTTC TTTTAATAAC CACTCATAAA TCCTCATAGA GTATTTGTTT TCAAAAGACT TAACATGTTC CAGATTATAT TTTATGAATT TTTTTAACTG GAAAAGATAA GGCAATATCT CTTCACTAAA AACTAATTCT AATTTTTCGC TTGAGAACTT GGCATAGTTT GTCCACTGGA AAATCTCAAA GCCTTTAACC AAAGGATTCC TGATTTCCAC AGTTCTCGTC ATCAGCTCTC TGGTTGCTTT AGCTAATACA CCATAAGCAT TTTCCCTACT GATGTTCATC ATCTGAGCGT ATTGGTTATA AGTGAACGAT ACCGTCCGTT CTTTCCTTGT AGGGTTTTCA ATCGTGGGGT TGAGTAGTGC CACACAGCAT AAAATTAGCT TGGTTTCATG CTCCGTTAAG TCATAGCGAC TAATCGCTAG TTCATTTGCT TTGAAAACAA CTAATTCAGA CATACATCTC AATTGGTCTA GGTGATTTTA ATCACTATAC CAATTGAGAT GGGCTAGTCA ATGATAATTA CTAGTCCTTT TCCTTTGAGT TGTGGGTATC TGTAAATTCT GCTAGACCTT TGCTGGAAAA CTTGTAAATT CTGCTAGACC CTCTGTAAAT TCCGCTAGAC CTTTGTGTGT TTTTTTTGTT TATATTCAAG TGGTTATAAT TTATAGAATA AAGAAAGAAT AAAAAAAGAT AAAAAGAATA GATCCCAGCC CTGTGTATAA CTCACTACTT TAGTCAGTTC CGCAGTATTA CAAAAGGATG TCGCAAACGC TGTTTGCTCC TCTACAAAAC AGACCTTAAA ACCCTAAAGG CTTAAGTAGC ACCCTCGCAA GCTCGGTTGC GGCCGCAATC GGGCAAATCG CTGAATATTC CTTTTGTCTC CGACCATCAG GCACCTGAGT CGCTGTCTTT TTCGTGACAT TCAGTTCGCT GCGCTCACGG CTCTGGCAGT GAATGGGGGT AAATGGCACT ACAGGCGCCT TTTATGGATT CATGCAAGGA AACTACCCAT AATACAAGAA AAGCCCGTCA CGGGCTTCTC AGGGCGTTTT ATGGCGGGTC TGCTATGTGG TGCTATCTGA CTTTTTGCTG TTCAGCAGTT CCTGCCCTCT GATTTTCCAG TCTGACCACT TCGGATTATC CCGTGACAGG TCATTCAGAC TGGCTAATGC ACCCAGTAAG GCAGCGGTAT CATCAACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTG
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