pDC316-mCMV-DsRED2-Linker-SIDT2(rat)-3×FLAG
$99.00
In stock
SKU
PL60041
NM_001108142.1;RGD1308311
100μg liquid plasmid(Instantly):$120
| name | pDC316-mCMV-DsRED2-Linker-SIDT2(rat)-3×FLAG | another name | apDC316-mCMV-DsRED2-Linker-SIDT2(rat)-3×FLAG |
| Insert Size (bp) | 2934bp | Plasmid host | Mammalian cell,Adenovirus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Rat | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | mCMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGCTCTAGA GATATACTGA GTCATTAGGG ACTTTCCAAT GGGTTTTGCC CAGTACATAA GGTCAATAGG GGTGAATCAA CAGGAAAGTC CCATTGGAGC CAAGTACACT GAGTCAATAG GGACTTTCCA TTGGGTTTTG CCCAGTACAA AAGGTCAATA GGGGGTGAGT CAATGGGTTT TTCCCATTAT TGGCACGTAC ATAAGGTCAA TAGGGGTGAG TCATTGGGTT TTTCCAGCCA ATTTAATTAA AACGCCATGT ACTTTCCCAC CATTGACGTC AATGGGCTAT TGAAACTAAT GCAACGTGAC CTTTAAACGG TACTTTCCCA TAGCTGATTA ATGGGAAAGT ACCGTTCTCG AGCCAATACA CGTCAATGGG AAGTGAAAGG GCAGCCAAAA CGTAACACCG CCCCGGTTTT CCCCTGGAAA TTCCATATTG GCACGCATTC TATTGGCTGA GCTGCGTTCT ACGTGGGTAT AAGAGGCGCG ACCAGCGTCG GTACCGTCGC AGTCTTCGGT CTGACCACCG TAGAACGCAG ATCGAATTCA TGGCCTCCTC CGAGAACGTC ATCACCGAGT TCATGCGCTT CAAGGTGCGC ATGGAGGGCA CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCC ACAACACCGT GAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC TGGGACATCC TGTCCCCCCA GTTCCAGTAC GGCTCCAAGG TGTACGTGAA GCACCCCGCC GACATCCCCG ACTACAAGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGCGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCTGC TTCATCTACA AGGTGAAGTT CATCGGCGTG AACTTCCCCT CCGACGGCCC CGTGATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCACC GAGCGCCTGT ACCCCCGCGA CGGCGTGCTG AAGGGCGAGA CCCACAAGGC CCTGAAGCTG AAGGACGGCG GCCACTACCT GGTGGAGTTC AAGTCCATCT ACATGGCCAA GAAGCCCGTG CAGCTGCCCG GCTACTACTA CGTGGACGCC AAGCTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAGCAGTA CGAGCGCACC GAGGGCCGCC ACCACCTGTT CCTGGGAGGA GGGGGAAGCA AGCTTACGTC AGCGGGCGGG CGCAGCCCCG CTGTTTACCG CGGCGCGGCG CCTGGTGGCC CCGAGTTCTG TGCCGCGTCC GCCGCCCTTA AAGGGCCCGC GTCCGGGAAG CCTACGGGCC AGGGCCACCG CCGCCTCGGC TCTCCGGGCG CGAGCGGACG CCGGGTCTCC AGCCGGGCTG GGCCGGGCTG GGGTTGCGGG TCGCGGTGCC GCCCACGCGA GCCGGGGTCC GCGCGAGGCC CAGGAGCGGC TCCAGGGAGG GGACGCGTCC TATCTCAGGC CGGGGTTAAA GTTCTGGTCC CGGTGAGATG CTGGAAGCTG CTGCGGCAGC CGCAACGCGC CCGGTCGCCG TCCCGTCGCC AGCCCCCGCC GCCCCGGGCC ATGATCGCCT GGCGTCTGCC CTTGTGCGTG CTCCTGGTGG CCGCCGTCGA GAGCCACCTG GGGGCTCTGG GGCCCAAGAA CGTCTCGCAG AAAGACGCGG AGTTTGAGCG CACCTACGCG GACGACGTCA ACAGCGAGCT AGTCAACATC TACACCTTCA ACCACACCGT GACCCGCAAC CGGACCGAGG GCGTGCGAGT GTCTGTGAAC GTCCTGAACA AACAGAAAGG GGCGCCTTTG CTGTTCGTGG TCCGCCAGAA GGAGGCTGTT GTGTCCTTCC AGGTGCCCCT AATCCTGCGA GGGCTATACC AGCGTAAGTA CCTCTACCAA AAAGTTGAAC GAACTCTGTG TCAGCCCCCC ACCAAGAATG AGTCTGAGAT CCAGTTTTTC TATGTGGATG TGTCTACCCT GTCACCAGTC AATACCACTT ACCAGCTCCG AGTCAACCGT GTGGACAATT TCGTGCTCAG GACTGGGGAG CTGTTTACCT TCAATACCAC TGCAGCCCAA CCCCAGTACT TCAAATACGA GTTTCCTGAT GGTGTGGACT CTGTCATTGT CAAGGTGACC TCCAAGAAGG CCTTCCCCTG CTCTGTCATC TCCATCCAGG ATGTCCTGTG CCCAGTCTAT GATCTTGACA ACAATGTAGC CTTCATCGGC ATGTACCAGA CAATGACTAA GAAGGCAGCC ATCACTGTGC AGAGGAAAGA CTTCCCCAGC AACAGTTTTT ATGTGGTGGT GGTGGTGAAG ACTGAGGACC AGGCCTGCGG GGGGTCCTTG CCCTTCTACC CTTTCGTGGA AGATGAACCA GTGGATCAAG GGCACCGTCA GAAAACCCTG TCAGTGCTGG TCTCTCAGGC TGTCACTTCT GAGGCCTACG TTGGTGGGAT GCTCTTTTGC CTGGGCATAT TCCTGTCGTT CTACCTGCTG ACTGTGCTGC TGGCCTGTTG GGAGAACTGG AGGCAAAGGA AGAAGACCTT GCTGGTGGCC ATAGACCGAG CCTGCCCAGA AAGCGGTCAC CCTCGGGTCT TGGCTGATTC GTTTCCTGGC AGTGCCCCCT ATGAGGGTTA CAACTATGGC TCCTTTGAGA ATGGTTCCGG ATCCACTGAC GGGTTGGTTG AAAGCACAGG TTCAGGGGAC CTCTCCTACA GTTACCAGGG GCACGACCAG TTCAAGCGGC GCCTTCCCTC TGGCCAGATG CGGCAGCTGT GCATTGCCAT GGACCGCTCC TTTGACCCAG TGGGAGCTCG GCCACGACTG GACTCCATGA GCTCCGTGGA AGAGGATGAC TATGACACAC TGACTGACAT CGACTCAGAC AAAAACGTCA TTCGAACCAA GCAATACCTC TGTGTGGCTG ATCTGGCACG AAAGGACAAA CGTGTTTTAC GGAAAAAGTA CCAGATTTAC TTCTGGAACA TAGCCACCAT TGCAGTCTTC TACGCCCTTC CTGTGGTGCA GCTGGTGATC ACCTACCAGA CGGTGGTGAA TGTCACGGGG AACCAGGACA TCTGCTACTA CAACTTCCTC TGTGCCCACC CACTGGGCAA CCTCAGCGCC TTCAACAACA TCCTCAGCAA CCTGGGGTAC ATTCTGCTTG GTCTGCTCTT CCTGCTCATC ATCCTGCAGC GGGAGATCAA TCATAACCGG GCCCTGCTGA GAAATGACCT CTATGCTCTG GAGTGTGGGA TCCCCAAACA CTTTGGTCTA TTTTATGCCA TGGGCACAGC GTTGATGATG GAGGGGCTAC TGAGTGCCTG TTACCACGTC TGCCCCAACT ACACCAACTT CCAGTTCGAT ACCTCGTTCA TGTACATGAT TGCTGGCCTC TGCATGCTGA AGCTCTACCA GAAGCGGCAC CCAGATATCA ACGCCAGCGC CTACAGCGCA TACGCCTGCC TGGCCATTGT GATCTTCTTC TCCGTCCTGG GTGTGGTTTT TGGCAAAGGG AACACAGCCT TCTGGATCGT CTTCTCAGTC ATCCATATCA TTTCCACGCT TCTCCTCAGC ACTCAGCTGT ATTACATGGG CCGCTGGAAG CTGGACTCCG GAATCTTCCG CCGCATCCTT CATGTGCTCT ACACAGACTG CATCCGGCAG TGCAGCGGGC CCCTTTACAC GGACCGCATG GTGCTTCTGG TCATGGGCAA CATTATCAAC TGGTCGCTGG CTGCCTACGG ACTCATCATG CGCCCAAATG ACTTTGCGTC CTACTTGCTG GCAATTGGCA TCTGCAACTT GCTGCTGTAT TTTGCTTTCT ACATCATTAT GAAGCTCCGG AGCGGCGAGA GGATCAAGCT CATCCCTCTG CTCTGCATCG TGTGCACCTC CGTGGTCTGG GGCTTCGCAC TCTTCTTCTT CTTCCAGGGA CTCAGCACGT GGCAGAAAAC CCCCGCAGAG TCCAGGGAGC ACAACCGAGA CTGCATCCTC CTTGACTTCT TTGATGACCA CGACATCTGG CACTTCCTGT CCTCCATTGC TATGTTTGGG TCCTTCCTGG TTTTATTGAC GTTGGATGAC GACTTGGACA CTGTACAGCG GGACAAGATC TATGTCTTCG GATCCGATTA CAAGGATGAC GACGATAAGG ACTATAAGGA CGATGATGAC AAGGACTACA AAGATGATGA CGATAAATGA GTCGACTTCG AGCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCGT CTAGCATCGA AGATCCAATA ACTTCGTATA GCATACATTA TACGAAGTTA TAAGTAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA CACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC TCTAGGCAAA ATAGCACCCT CCCGCTCCAG AACAACATAC AGCGCTTCAC AGCGGCAGCC TAACAGTCAG CCTTACCAGT AAAAAAGAAA ACCTATTAAA AAAACACCAC TCGACACGGC ACCAGCTCAA TCAGTCACAG TGTAAAAAAG GGCCAAGTGC AGAGCGAGTA TATATAGGAC TAAAAAATGA CGTAACGGTT AAAGTCCACA AAAAACACCC AGAAAACCGC ACGCGAACCT ACGCCCAGAA ACGAAAGCCA AAAAACCCAC AACTTCCTCA AATCGTCACT TCCGTTTTCC CACGTTACGT AACTTCCCAT TTTAAGAAAA CTACAATTCC CAACACATAC AAGTTACTCC GCCCTAAAAC CTACGTCACC CGCCCCGTTC CCACGCCCCG CGCCACGTCA CAAACTCCAC CCCCTCATTA TCATATTGGC TTCAATCCAA AATAAGGTAT A
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