pCMV-IPO8(human)-3×HA-SV40-Neo
$99.00
In stock
SKU
PL60020
NM_006390.4;RANBP8
100μg liquid plasmid(Instantly):$120
| name | pCMV-IPO8(human)-3×HA-SV40-Neo | another name | apCMV-IPO8(human)-3×HA-SV40-Neo |
| Insert Size (bp) | 3111bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CGCCACCATG GACCTCAACC GGATCATCCA GGCGCTGAAG GGCACCATCG ACCCGAAGTT GCGGATTGCA GCCGAGAACG AGCTCAACCA GTCCTACAAG ATTATCAATT TTGCCCCCAG TTTACTTCGG ATTATAGTCT CTGACCATGT GGAATTCCCA GTACGACAGG CAGCTGCCAT TTACCTGAAG AACATGGTGA CACAATACTG GCCAGATCGA GAACCTCCAC CAGGAGAAGC AATATTTCCA TTCAACATTC ACGAAAACGA TCGCCAGCAA ATACGTGATA ACATTGTGGA AGGAATAATT CGGTCTCCAG ATTTAGTGAG AGTCCAATTA ACAATGTGTC TCCGTGCCAT CATAAAACAT GATTTTCCTG GTCACTGGCC AGGAGTGGTC GACAAGATAG ACTATTACTT GCAATCACAG AGCAGTGCAA GCTGGCTTGG CAGTTTATTA TGCCTGTATC AACTGGTGAA GACATATGAA TATAAGAAAG CAGAAGAGAG AGAACCTCTT ATAATAGCAA TGCAGATATT CCTGCCTCGT ATTCAGCAAC AAATTGTTCA GCTCCTTCCT GATTCCTCCT ATTATTCTGT ATTACTGCAG AAACAAATTC TGAAAATCTT TTATGCACTT GTTCAGTATG CATTGCCTCT TCAGCTAGTG AATAACCAAA CCATGACAAC ATGGATGGAG ATCTTCCGAA CTATTATCGA CAGGACCGTT CCTCCTGAGA CTCTGCACAT TGATGAGGAT GATAGACCAG AACTGGTATG GTGGAAGTGT AAGAAGTGGG CACTGCATAT TGTAGCTCGG CTCTTTGAAC GATATGGAAG CCCAGGAAAT GTCACAAAAG AATACTTTGA ATTTTCTGAA TTCTTTTTGA AAACCTATGC AGTGGGCATT CAGCAGGTGC TACTAAAAAT TTTAGATCAA TATAGACAGA AAGAATATGT AGCTCCCCGT GTTCTTCAGC AAGCATTCAA CTATCTCAAC CAAGGGGTGG TTCATTCTAT AACCTGGAAG CAGATGAAGC CACACATACA GAATATCTCT GAAGATGTGA TTTTTTCTGT GATGTGTTAT AAAGATGAGG ATGAAGAGCT GTGGCAAGAA GATCCATATG AGTATATAAG GATGAAATTT GATATTTTTG AAGATTATGC TTCTCCCACC ACAGCAGCCC AGACTCTCTT ATATACTGCT GCAAAGAAAA GAAAAGAGGT GTTGCCAAAA ATGATGGCAT TCTGTTATCA AATCCTGACA GACCCGAACT TTGACCCTAG GAAGAAAGAT GGAGCCCTGC ATGTGATTGG TTCCCTAGCT GAGATTTTAC TGAAGAAGAG TTTATTCAAG GACCAAATGG AGCTGTTTCT ACAAAATCAT GTATTTCCAT TATTATTGTC TAACCTGGGA TATCTTCGAG CTAGATCTTG CTGGGTACTT CATGCATTTA GTTCTTTGAA GTTCCATAAT GAGCTCAATC TAAGAAATGC CGTTGAATTA GCGAAGAAGA GCCTGATTGA AGATAAAGAG ATGCCTGTCA AAGTTGAAGC TGCCCTTGCT CTTCAGTCTT TAATTTCTAA CCAGATACAA GCTAAGGAAT ATATGAAGCC ACATGTGAGG CCTATTATGC AGGAACTGTT GCACATTGTT AGAGAGACAG AAAATGATGA TGTTACTAAT GTCATCCAGA AGATGATATG TGAATACAGT CAAGAGGTAG CCTCAATTGC TGTTGATATG ACCCAACACT TGGCTGAGAT ATTTGGCAAA GTTCTTCAAA GTGATGAATA TGAAGAAGTT GAAGACAAAA CAGTAATGGC TATGGGAATT TTACATACCA TTGATACTAT CTTAACAGTT GTAGAAGATC ATAAAGAGAT TACCCAGCAG TTAGAGAATA TCTGTCTACG GATCATTGAT CTTGTTCTGC AGAAACATGT AATTGAATTC TATGAAGAAA TTCTTTCCCT GGCATACAGT TTAACCTGCC ACAGTATTTC CCCTCAAATG TGGCAGCTTC TAGGTATACT ATATGAAGTG TTTCAGCAGG ATTGCTTTGA ATACTTTACA GACATGATGC CTCTCCTGCA TAATTATGTG ACAATAGATA CAGATACCTT ACTATCAAAT GCAAAACATT TAGAAATTCT TTTTACAATG TGTAGGAAGG TACTATGTGG AGATGCAGGA GAAGATGCAG AGTGTCATGC AGCTAAACTT CTGGAAGTCA TCATTCTTCA GTGCAAAGGA AGGGGAATTG ATCAGTGCAT TCCACTCTTC GTTCAACTTG TTTTGGAGAG ATTAACTCGA GGGGTCAAAA CTAGTGAGCT TCGTACTATG TGTCTTCAGG TTGCAATTGC TGCCTTGTAC TACAACCCTG ATTTGCTGCT ACATACTTTA GAACGAATTC AGTTGCCTCA CAACCCTGGA CCTATCACTG TACAGTTTAT AAATCAATGG ATGAATGATA CAGATTGTTT TCTTGGGCAT CATGACCGGA AGATGTGTAT AATAGGACTG AGTATCCTTT TGGAATTGCA AAATCGACCT CCTGCAGTAG ATGCTGTGGT GGGACAGATT GTTCCCTCAA TTCTTTTCCT TTTCCTTGGC CTAAAGCAGG TCTGTGCTAC TAGACAACTG GTAAACCGGG AAGATCGTTC AAAAGCAGAG AAAGCTGATA TGGAAGAAAA TGAGGAGATT TCAAGTGATG AAGAGGAGAC AAATGTAACT GCTCAAGCAA TGCAGTCAAA TAATGGAAGA GGTGAAGATG AGGAGGAGGA AGATGATGAC TGGGATGAAG AAGTATTGGA AGAAACCGCG CTTGAGGGGT TCAGTACTCC ACTTGACCTT GACAATAGTG TGGATGAATA TCAGTTTTTT ACACAAGCTC TGATAACTGT GCAGAGTCGA GATGCAGCCT GGTACCAGCT GCTGATGGCA CCACTCAGCG AGGATCAGAG GACAGCACTG CAGGAGGTGT ACACACTGGC AGAGCACCGA CGGACGGTGG CAGAGGCAAA GAAGAAGATT GAACAACAGG GAGGCTTCAC CTTTGAAAAC AAAGGAGTCC TCTCCGCATT TAATTTTGGG ACTGTGCCCA GCAACAACCT CGAGTACCCA TACGACGTAC CTGATTATGC AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT TATGATGTCC CAGACTACGC ATGAGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGT
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