pTac-tnpA-Kan
$84.00
In stock
SKU
PL59511
100μg liquid plasmid(Instantly):$105
| name | pTac-tnpA-Kan | another name | apTac-tnpA-Kan |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pSC101 | |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAGAGCTCGC TTGGACTCCT GTTGATAGAT CCAGTAATGA CCTCAGAACT CCATCTGGAT TTGTTCAGAA CGCTCGGTTG CCGCCGGGCG TTTTTTATTG GTGAGAATCC AAGCACTAGG GACAGTAAGA CGGGTAAGCC TGTTGATGAT ACCGCTGCCT TACTGGGTGC ATTAGCCAGT CTGAATGACC TGTCACGGGA TAATCCGAAG TGGTCAGACT GGAAAATCAG AGGGCAGGAA CTGCTGAACA GCAAAAAGTC AGATAGCACC ACATAGCAGA CCCGCCATAA AACGCCCTGA GAAGCCCGTG ACGGGCTTTT CTTGTATTAT GGGTAGTTTC CTTGCATGAA TCCATAAAAG GCGCCTGTAG TGCCATTTAC CCCCATTCAC TGCCAGAGCC GTGAGCGCAG CGAACTGAAT GTCACGAAAA AGACAGCGAC TCAGGTGCCT GATGGTCGGA GACAAAAGGA ATATTCAGCG ATTTGCCCGA GCTTGCGAGG GTGCTACTTA AGCCTTTAGG GTTTTAAGGT CTGTTTTGTA GAGGAGCAAA CAGCGTTTGC GACATCCTTT TGTAATACTG CGGAACTGAC TAAAGTAGTG AGTTATACAC AGGGCTGGGA TCTATTCTTT TTATCTTTTT TTATTCTTTC TTTATTCTAT AAATTATAAC CACTTGAATA TAAACAAAAA AAACACACAA AGGTCTAGCG GAATTTACAG AGGGTCTAGC AGAATTTACA AGTTTTCCAG CAAAGGTCTA GCAGAATTTA CAGATACCCA CAACTCAAAG GAAAAGGACT AGTAATTATC ATTGACTAGC CCATCTCAAT TGGTATAGTG ATTAAAATCA CCTAGACCAA TTGAGATGTA TGTCTGAATT AGTTGTTTTC AAAGCAAATG AACTAGCGAT TAGTCGCTAT GACTTAACGG AGCATGAAAC CAAGCTAATT TTATGCTGTG TGGCACTACT CAACCCCACG ATTGAAAACC CTACAAGGAA AGAACGGACG GTATCGTTCA CTTATAACCA ATACGCTCAG ATGATGAACA TCAGTAGGGA AAATGCTTAT GGTGTATTAG CTAAAGCAAC CAGAGAGCTG ATGACGAGAA CTGTGGAAAT CAGGAATCCT TTGGTTAAAG GCTTTGAGAT TTTCCAGTGG ACAAACTATG CCAAGTTCTC AAGCGAAAAA TTAGAATTAG TTTTTAGTGA AGAGATATTG CCTTATCTTT TCCAGTTAAA AAAATTCATA AAATATAATC TGGAACATGT TAAGTCTTTT GAAAACAAAT ACTCTATGAG GATTTATGAG TGGTTATTAA AAGAACTAAC ACAAAAGAAA ACTCACAAGG CAAATATAGA GATTAGCCTT GATGAATTTA AGTTCATGTT AATGCTTGAA AATAACTACC ATGAGTTTAA AAGGCTTAAC CAATGGGTTT TGAAACCAAT AAGTAAAGAT TTAAACACTT ACAGCAATAT GAAATTGGTG GTTGATAAGC GAGGCCGCCC GACTGATACG TTGATTTTCC AAGTTGAACT AGATAGACAA ATGGATCTCG TAACCGAACT TGAGAACAAC CAGATAAAAA TGAATGGTGA CAAAATACCA ACAACCATTA CATCAGATTC CTACCTACAT AACGGACTAA GAAAAACACT ACACGATGCT TTAACTGCAA AAATTCAGCT CACCAGTTTT GAGGCAAAAT TTTTGAGTGA CATGCAAAGT AAGTATGATC TCAATGGTTC GTTCTCATGG CTCACGCAAA AACAACGAAC CACACTAGAG AACATACTGG CTAAATACGG AAGGATCTGA GGTTCTTATG GCTCTTGTAT CTATCAGTGA AGCATCAAGA CTAACAAACA AAAGTAGAAC AACTGTTCAC CGTTACATAT CAAAGGGAAA ACTGTCCATA TGCACAGATG AAAACGGTGT AAAAAAGATA GATACATCAG AGCTTTTACG AGTTTTTGGT GCATTCAAAG CTGTTCACCA TGAACAGATC GACAATGTAA CAGATGAACA GCATGTAACA CCTAATAGAA CAGGTGAAAC CAGTAAAACA AAGCAACTAG AACATGAAAT TGAACACCTG AGACAACTTG TTACAGCTCA ACAGTCACAC ATAGACAGCC TGAAACAGGC GATGCTGCTT ATCGAATCAA AGCTGCCGAC AACACGGGAG CCAGTGACGC CTCCCGTGGG GAAAAAATCA TGGCAATTCT GGAAGAAATA GCGCTTTCAG CCGGCAAACC GGCTGAAGCC GGATCTGCGA TTCTGATAAC AAACTAGCAA CACCAGAACA GCCCGTTTGC GGGCAGCAAA ACCCGTACCC TAGGTCTAGG GCGGCGGATT TGTCCTACTC AGGAGAGCGT TCACCGACAA ACAACAGATA AAACGAAAGG CCCAGTCTTT CGACTGAGCC TTTCGTTTTA TTTGATGCCT CTAGAGCTTG CATGCCCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGTATAAT CCTTACTGCG TTAAAGGCTT TTCTTAGGAA AGTTGGCCAT TTCTTAATTC AGCCATTAAT TAAGAAATAT TAAGAATATT CCTGGCTATT TTCTCCTGTC AGAGTCTATT GTTTTAGCCT GAAAAGCTAA AAAACGTTAA CAAGCTTGCA TGCCTGCAGG TCGACTCTAG AGGATCCGAT TTTAATGCCC TGCGCCATCA GGTCTTTCGC GGCCAGAAAG CCATCCAGTT TGCTTTGCAG CGCTTCCCAA CCTTCCCACA GCGCACCCCA GCTCGCAATG CCGGTACGTT TGCTATCCAT AAAGCCGCCC AGACGCGCAA TCGCCATATA CGCCCATTGC AGGCTGCCCG CTTTTTCTTT GCGTTTGCGT TTGCCTTTAT CCAGATAGCC CAGCAGTTGG CATTCATCCG GGGTCAGCAC GGTTTCCGCG CTCTGGCTTT CAACGTGTTC CGCTTCTTTC AGCAGGCCCT GCGCACGCAG TGCTTGCGGC GGAGTAAAAG ATTCACGCAG TTGCAGCAGA CGCACCGCCA CAAAGCTCAG AATGCTCACC ATACGTTCCA GGTTATCCGG TTCTTCCATA CGCTGACGTT CCGCACCCGC ACCCGTTTTC CACGCTTTGT GAAATTCTTC AATGCGCCAA CGATGGGTAT AAATATCAAT CACACGCAGC GCTTGGGCCA GACTTTCCAC CGGCTCGCTG GTCAGCAGCA GCCATTTCAG CGGGGTTTCG CCTTTCGGCG GATTAATTTC TTCGGCCAGC ACCGCGTTCA GGGTAATGTT GCCCTGTTTC AGGGTAATAC GGCCGCTACG CAGGCTCAGG CTCGCTTTAC GCGCCGGACG GTTTTTACGT TTGCCACGTT TATCCACCAC GCCTTTCTGC GGAATGCTAA TCTGATAGCC GCCCAGTTCC GGCTGGTTTT TCAGGTGATC ATACAGATAC AGGCCGCTTT CCACATCTTT ACGCGGATGT TTGCTACGCA CCACAAAACG TTCGTTATGG GCCAGTTTAT CTTGCAGATA CGCATGAATA TCCGCTTCAC GATCGCACAC CGCAATCACG TTGCTCATCA TGCTGCCCAT TCTCAGACGC GAAGTTGCAG CAGCGGCCAG CCATTTGCCG CTTTCTTTTT CATCCGCATC CGCCGGATCA TCCGGACGCA TCCACCATTC TTGATGCAGC AGGCCCACGG TACGAAAGGT GGTCGCTTCC AGCAGCAGCA CGCTATGCAC CCACCAACCA CGGCTTTTAT CCTGAATGCT ACCCAGTTTG CCCAGTTCTT CCGCCACCTG ATGACGATAG CTCAGAGAGG TGGTATCTTC AATTGCCAGC AGTTCCGGAA ATTCCTGGGC CAGTTTCACG GTCTGCATGG CACCCGCTTT ACGAATCGCT TCCGCGCTCA CGTTCGGATT ACGAATAAAA CGATACGCGC CTTCCTGCAT GGCTTTGCTG CCTTCGCTGC TAATGGTAAT GCTTTTGCCG CTATATTTGG CCAGTTGCGC CGCAACATTC ACCAGACGCG CGGTACGACG CGGATCACCC AGCGCAGCAC TAGAAAACAC GCTTTTCGCC CAATCCGCCG CACGATGCAG TGCACTGGTA ATCATGAATT CCATGGTCTG TTTCCTGTGT GAAACATTAT ACGAGCCGAT GATTAATTGT CAACAGCTCA TTTCAGAATA TTTGCCAGAA CCGTTATGAT GTCGGCGCAA AAAACATTAT CCAGAACGGG AGTGCGCCTT GAGCGACACG AATTATGCAG TGATTTACGA CCTGCACAGC CATACCACAG CTTCCGATGG CTGCCTGACG CCAGAAGCAT TGGTGCACCG TGCAGTCGAT GATAAGCTGT CAAACCAGAT CAATTCGCGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCA CCATTCGATG GTGTCCGAGG TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCGGAATT GCCAGCTGGG GCGCCCTCTG GTAAGGTTGG GAAGCCCTGC AAAGTAAACT GGATGGCTTT CTTGCCGCCA AGGATCTGAT GGCGCAGGGG ATCAAGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TC
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