NM_175130.4;1110030C19Rik; AW047689; LTR; LTrpC-4
100μg liquid plasmid(Instantly):$134
| name | pCDH-CMV-TRPM4(mouse)-EF1a-turboRFP-T2A-Puro | another name | apCDH-CMV-TRPM4(mouse)-EF1a-turboRFP-T2A-Puro |
| Insert Size (bp) | 3639bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT AGAGCTAGCC ACCATGGTGG GGCCGGAGAA GGAGCAGAGC TGGATCCCTA AGATCTTCAG GAAGAAGGTG TGCACGACGT TCATAGTGGA CCTCTCCGAT GATGCGGGAG GGACCTTGTG CCAGTGTGGG CAACCTCGGG ATGCCCACCC TTCTGTGGCT GTGGAGGATG CCTTTGGGGC AGCCGTAGTT ACCGAGTGGA ACAGTGATGA GCACACCACG GAGAAGCCCA CAGATGCCTA TGGGGACCTG GACTTCACGT ACTCTGGCCG AAAGCACAGC AACTTTCTCC GGCTGTCTGA CCGCACGGAT CCGGCCACTG TGTACAGTCT CGTTACCCGC TCTTGGGGCT TTCGTGCCCC AAACTTGGTG GTGTCGGTGC TGGGGGGGTC GGGGGGCCCC GTGCTCCAGA CCTGGCTGCA GGACCTTCTG CGTCGCGGGC TGGTGAGAGC CGCCCAGAGC ACAGGGGCCT GGATCGTCAC TGGGGGACTG CACACAGGCA TTGGCCGGCA CGTGGGTGTG GCTGTAAGGG ACCACCAGAC AGCCAGCACT GGGAGCAGCA AGGTGGTGGC CATGGGTGTG GCCCCCTGGG GAGTGGTCCG GAACAGAGAC ATGCTGATCA ACCCCAAGGG CTCATTCCCC GCAAGGTACC GGTGGCGTGG TGACCCTGAG GATGGTGTTG AGTTCCCCCT GGACTATAAC TATTCTGCTT TCTTCTTGGT GGATGATGGC ACCTATGGCC GCCTGGGTGG TGAGAACCGC TTCCGCCTTC GGTTTGAGTC CTATGTGGCT CAGCAGAAGA CCGGAGTGGG AGGGACTGGA ATTGACATCC CAGTGCTCCT TCTTCTCATC GATGGAGATG AGAAGATGTT AAAGCGGATA GAGGATGCCA CCCAAGCTCA GCTCCCCTGC CTCTTGGTGG CGGGCTCTGG GGGTGCTGCA GACTGTCTGG TGGAGACCCT GGAAGATACT CTGGCCCCAG GGAGTGGGGG ACTTAGGCGA GGCGAAGCCC GGGATCGGAT TAGGCGATAC TTCCCTAAAG GAGACCCTGA GGTTCTTCAG GCCCAGGTAG AGAGGATCAT GACCCGAAAG GAGTTGCTGA CGGTCTATTC ATCAGAAGAC GGCTCCGAGG AGTTTGAGAC TATCGTTTTG AGGGCTCTTG TGAAAGCCTG TGGGAGCTCT GAGGCCTCAG CCTACCTGGA TGAGCTGCGT TTGGCTGTGG CTTGGAACCG CGTGGACATC GCCCAAAGTG AACTTTTCCG TGGGGACATC CAATGGCGGT CCTTCCACCT GGAGGCATCT CTCATGGATG CTCTGCTGAA TGACCGGCCT GAATTTGTGC GCCTGCTCAT CTCCCATGGC CTTAGCCTGG GACACTTCCT GACCCCCGTG CGTCTGGCCC AGCTGTACAG CGCGGTGTCC CCTAACTCAC TGATCCGAAA TCTTCTGGAC CAGGCGTCCC ATGCTAGTAG CAGCAAATCC CCTCCTGTAA ATGGAACTGT AGAGCTCCGG CCTCCCAATG TGGGGCAAGT GCTGAGGACT CTGTTGGGAG AAACGTGTGC ACCACGGTAC CCTGCCAGGA ACACCCGAGA CTCCTATCTG GGCCAGGACC ACAGAGAGAA TGACTCTCTG CTTATGGACT GGGCCAACAA GCAACCATCA ACGGATGCAA GCTTTGAACA AGCCCCCTGG AGTGACCTGC TTATTTGGGC TCTGTTGCTG AACCGGGCCC AGATGGCTAT CTATTTCTGG GAGAAGGGCT CCAACTCAGT GGCCTCTGCT CTTGGGGCTT GTCTTTTACT CCGAGTGATG GCTCGCCTAG AGTCGGAGGC TGAGGAGGCC GCGCGGCGGA AGGATCTTGC TGCCACGTTT GAAAGCATGA GCGTGGACCT CTTTGGAGAG TGTTACCACA ACAGTGAAGA GCGAGCAGCC CGCCTTCTTC TGCGCCGCTG TCCCCTCTGG GGAGAGGCCA CCTGCCTCCA GCTTGCCATG CAGGCTGATG CCCGAGCGTT CTTTGCCCAG GATGGAGTCC AGTCTCTTCT GACACAGAAG TGGTGGGGGG AGATGGACAG CACAACTCCC ATCTGGGCCC TGCTTCTCGC CTTCTTCTGC CCGCCTCTCA TCTACACCAA CCTTATCGTC TTCAGGAAGT CAGAGGAGGA GCCCACACAG AAGGATCTTG ACTTTGATAT GGACAGCAGC ATCAATGGAG CAGGTCCTCC TGGGACTGTA GAACCCTCTG CAAAGGTGGC CCTTGAGAGG CGGCAGCGGC GACGGCCAGG ACGTGCCCTC TGCTGTGGCA AGTTCTCCAA GCGCTGGTCG GACTTCTGGG GTGCCCCAGT GACTGCCTTC CTGGGTAATG TGGTCAGTTA CCTGCTGTTC CTGCTGCTGT TTGCCCACGT GTTGCTGGTG GATTTCCAGC CCACAAAGCC AAGTGTCTCC GAGCTGCTGC TGTACTTCTG GGCCTTCACG TTGCTGTGCG AGGAGCTGCG CCAGGGCCTG GGTGGTGGCT GGGGTAGCCT GGCCAGTGGG GGACGTGGTC CGGACAGAGC TCCCCTGCGC CATCGGCTGC ACCTCTACCT CTCAGATACC TGGAACCAAT GTGACCTGCT GGCACTCACC TGCTTCCTGC TGGGCGTAGG CTGCCGGCTG ACCCCTGGGC TCTTTGACTT GGGACGCACC GTCCTCTGCC TTGACTTCAT GATCTTCACA CTGCGCCTGC TGCACATCTT CACGGTGAAC AAGCAGCTGG GGCCCAAGAT TGTCATAGTG AGCAAAATGA TGAAGGATGT ATTCTTCTTC CTCTTCTTCC TCTGCGTGTG GCTTGTAGCT TATGGGGTGG CCACAGAGGG GATCCTGAGG CCCCAGGACC GCAGTCTACC GAGTATCCTG CGCAGGGTCT TCTACCGACC GTATCTGCAG ATCTTCGGAC AAATTCCCCA GGAGGAAATG GACGTGGCCC TCATGATTCC AGGTAACTGC TCAATGGAGC GGGGCTCCTG GGCTCATCCG GAGGGGCCCG TCGCAGGCTC CTGTGTGTCC CAGTATGCCA ACTGGCTGGT GGTGTTGCTC CTTATCGTCT TCTTGCTGGT GGCCAATATC CTGTTGCTCA ACCTGCTCAT CGCCATGTTC AGCTACACCT TCAGCAAAGT GCACGGCAAC AGCGACCTCT ACTGGAAGGC ACAGCGCTAC AGCCTCATCC GGGAATTCCA TTCGAGGCCT GCCCTGGCCC CACCCCTCAT CATCATCTCT CATGTGCGCC TCCTCATCAA GTGGCTGCGC AGGTGTCGCA GGTGTCGCAG GGCTAACTTG CCCGCCTCCC CAGTCTTCGA GCACTTCCGT GTCTGTCTCT CTAAGGAAGC AGAGCGGAAG CTGCTGACTT GGGAGTCTGT GCACAAAGAG AACTTCCTGT TGGCACAAGC TCGGGACAAG CGAGACAGTG ACTCGGAGCG TTTGAAACGC ACGTCTCAGA AGGTGGACAC TGCACTGAAG CAGCTGGGAC AGATCAGAGA GTACGATCGG CGTCTGAGGG GGCTGGAGAG AGAGGTCCAA CACTGTTCTC GAGTCCTGAC TTGGATGGCT GAGGCCCTTA GCCACTCTGC CTTGCTGCCT CCAGGGGCCC CACCCCCTCC GAGCCCCACT GGTTCCAAAG ACGATTACAA GGACGACGAT GACAAGTGAG ATCCGCGGCC GCAAGGATCT GCGATCGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACGGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT TGCCGCCAGA ACACAGCTGA AGCTTCGAGG GGCTCGCATC TCTCCTTCAC GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT ACAGATCCAA GCTGTGACCG GCGCCTACGC TAGACGCCAC CATGAGCGAG CTGATCAAGG AGAACATGCA CATGAAGCTG TACATGGAGG GCACCGTGAA CAACCACCAC TTCAAGTGCA CATCCGAGGG CGAAGGCAAG CCCTACGAGG GCACCCAGAC CATGAAGATC AAGGTGGTCG AGGGCGGCCC TCTCCCCTTC GCCTTCGACA TCCTGGCTAC CAGCTTCATG TACGGCAGCA AAGCCTTCAT CAACCACACC CAGGGCATCC CCGACTTCTT TAAGCAGTCC TTCCCTGAGG GCTTCACATG GGAGAGAATC ACCACATACG AAGACGGGGG CGTGCTGACC GCTACCCAGG ACACCAGCTT CCAGAACGGC TGCATCATCT ACAACGTCAA GATCAACGGG GTGAACTTCC CATCCAACGG CCCTGTGATG CAGAAGAAAA CACGCGGCTG GGAGGCCAAC ACCGAGATGC TGTACCCCGC TGACGGCGGC CTGAGAGGCC ACAGCCAGAT GGCCCTGAAG CTCGTGGGCG GGGGCTACCT GCACTGCTCC TTCAAGACCA CATACAGATC CAAGAAACCC GCTAAGAACC TCAAGATGCC CGGCTTCCAC TTCGTGGACC ACAGACTGGA AAGAATCAAG GAGGCCGACA AAGAGACCTA CGTCGAGCAG CACGAGATGG CTGTGGCCAA GTACTGCGAC CTCCCTAGCA AACTGGGGCA CAGAGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC CCGGCCCTAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAAAT AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC TAGCTATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGACTTTT GCAGAGACGG CCCAAATTCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTG
