NM_014795.4;HSPC082; SIP-1; SIP1; SMADIP1; ZFHX1B
100μg liquid plasmid(Instantly):$120
| name | pEnCMV-ZEB2(human)-3×HA-SV40-Neo | another name | apEnCMV-ZEB2(human)-3×HA-SV40-Neo |
| Insert Size (bp) | 3642bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CGCCACCATG AAGCAGCCGA TCATGGCGGA TGGCCCCCGG TGCAAGAGGC GCAAACAAGC CAATCCCAGG AGGAAAAACG TGGTGAACTA TGACAATGTA GTGGACACAG GTTCTGAAAC AGATGAGGAA GACAAGCTTC ATATTGCTGA GGATGACGGT ATTGCCAACC CTCTGGACCA GGAGACGAGT CCAGCTAGTG TGCCCAACCA TGAGTCCTCC CCACACGTGA GCCAAGCTCT GTTGCCAAGA GAGGAAGAGG AAGATGAAAT AAGGGAGGGT GGAGTGGAAC ACCCCTGGCA CAACAACGAG ATTCTACAAG CCTCTGTAGA TGGTCCAGAA GAAATGAAGG AAGACTATGA CACTATGGGG CCAGAAGCCA CGATCCAGAC CGCAATTAAC AATGGTACAG TGAAGAATGC AAATTGCACA TCAGATTTTG AGGAATACTT TGCCAAAAGA AAACTGGAGG AACGCGATGG TCATGCAGTC AGCATCGAGG AGTACCTTCA GCGCAGTGAC ACAGCCATTA TTTACCCAGA AGCCCCTGAG GAGCTGTCTC GCCTTGGCAC GCCAGAGGCC AATGGGCAAG AAGAAAATGA CCTGCCACCT GGAACTCCAG ATGCTTTTGC CCAACTGCTG ACCTGCCCCT ACTGCGACCG GGGCTACAAG CGCTTGACAT CACTGAAGGA GCACATCAAG TACCGCCACG AGAAGAATGA AGAGAACTTT TCCTGCCCTC TCTGTAGCTA CACGTTTGCC TACCGCACCC AGCTCGAGCG GCATATGGTG ACACACAAGC CAGGGACAGA TCAGCACCAA ATGCTAACCC AAGGAGCAGG TAATCGCAAG TTCAAATGCA CAGAGTGTGG CAAGGCCTTC AAATATAAAC ACCATCTGAA AGAACACCTG CGAATTCACA GTGGTGAAAA ACCTTACGAG TGCCCAAACT GCAAGAAACG TTTCTCCCAT TCTGGTTCCT ACAGTTCGCA CATCAGCAGC AAGAAATGTA TTGGTTTAAT CTCTGTAAAT GGCCGAATGA GAAACAATAT CAAGACGGGT TCTTCCCCTA ATTCTGTTTC TTCTTCTCCT ACTAATTCAG CCATTACCCA GTTAAGAAAC AAGTTGGAGA ATGGAAAACC ACTTAGTATG TCTGAACAGA CAGGCTTACT TAAAATTAAA ACAGAACCAC TAGACTTCAA TGACTATAAA GTTCTTATGG CTACACACGG GTTTAGTGGC ACTAGTCCCT TTATGAATGG TGGGCTTGGA GCCACCAGCC CTTTAGGAGT TCATCCATCT GCTCAGAGTC CAATGCAGCA CTTAGGTGTA GGGATGGAAG CCCCTTTACT TGGGTTTCCC ACCATGAATA GTAATTTAAG TGAGGTACAA AAGGTTCTAC AGATTGTGGA CAATACTGTT TCCAGGCAAA AAATGGACTG CAAGGCTGAA GAAATTTCAA AGTTGAAAGG TTATCACATG AAGGATCCAT GCTCTCAACC TGAGGAACAA GGAGTTACTT CTCCTAATAT TCCGCCTGTC GGTCTTCCGG TAGTGAGTCA TAATGGTGCC ACTAAAAGTA TTATTGACTA TACGTTGGAA AAAGTCAATG AAGCCAAAGC TTGCCTCCAG AGCTTGACTA CTGACTCAAG GAGACAGATC AGTAATATAA AGAAAGAGAA GCTACGTACT TTAATAGATT TGGTCACTGA TGACAAAATG ATTGAGAACC ACAACATATC CACTCCATTT TCATGCCAGT TCTGTAAAGA AAGTTTTCCT GGCCCCATCC CTTTGCATCA GCATGAACGT TACCTTTGTA AGATGAATGA AGAGATCAAG GCGGTCCTGC AGCCTCATGA AAACATAGTC CCCAACAAAG CCGGAGTTTT TGTTGATAAT AAAGCCCTCC TCTTGTCATC TGTACTTTCT GAGAAAGGAA TGACAAGCCC CATCAACCCA TACAAGGACC ACATGTCTGT ACTCAAAGCA TACTATGCTA TGAACATGGA GCCCAACTCC GATGAACTGC TGAAAATTTC CATTGCTGTG GGCCTTCCTC AGGAATTTGT GAAGGAATGG TTTGAACAAC GAAAAGTCTA CCAGTACTCA AATTCCAGGT CCCCATCCCT GGAAAGAAGC TCCAAGCCGT TAGCTCCCAA CAGTAACCCT CCCACAAAAG ACTCTTTATT ACCCAGGTCT CCTGTAAAAC CTATGGACTC CATAACATCA CCATCTATAG CAGAACTCCA CAACAGTGTT ACGAATTGTG ATCCTCCTCT CAGGCTAACA AAACCTTCCC ATTTTACCAA TATTAAACCA GTTGAAAAAT TGGACCACTC CAGGAGTAAT ACTCCTTCTC CCTTAAATCT TTCCTCCACA TCTTCTAAAA ACTCCCACAG TAGTTCATAC ACTCCAAACA GCTTCTCTTC TGAGGAGCTC CAGGCTGAGC CTTTAGACTT GTCATTACCA AAACAAATGA AAGAACCCAA AAGTATTATA GCCACAAAGA ACAAAACAAA AGCTAGTAGC ATCAGTTTAG ATCATAACAG TGTTTCTTCC TCATCTGAAA ACTCAGATGA GCCTCTGAAC TTGACTTTTA TCAAGAAGGA ATTTTCAAAT TCAAATAATC TGGACAACAA AAGCACTAAC CCAGTGTTCA GCATGAACCC ATTTAGTGCC AAACCTTTAT ACACAGCTCT TCCACCTCAA AGCGCATTTC CCCCTGCTAC TTTCATGCCA CCAGTCCAGA CCAGTATTCC TGGGCTACGA CCATACCCAG GACTGGATCA GATGAGCTTC CTACCACATA TGGCCTACAC CTACCCAACT GGAGCAGCTA CTTTTGCTGA TATGCAGCAA AGGAGAAAGT ACCAGCGGAA ACAAGGATTT CAGGGAGAAT TGCTTGATGG AGCACAAGAC TACATGTCAG GCCTAGATGA TATGACAGAC TCCGACTCCT GTCTGTCTCG CAAAAAGATC AAGAAGACAG AGAGTGGCAT GTATGCATGT GACTTATGTG ACAAGACATT CCAGAAAAGC AGTTCCCTTC TGCGACATAA ATACGAACAC ACAGGAAAAA GACCACATCA GTGTCAGATT TGTAAGAAAG CGTTTAAACA CAAGCACCAC CTTATCGAGC ACTCAAGGCT TCACTCGGGC GAGAAGCCCT ATCAGTGTGA TAAATGTGGC AAGCGCTTCT CACACTCGGG CTCGTACTCG CAGCACATGA ATCACAGGTA TTCCTACTGC AAGCGGGAGG CGGAGGAGCG GGAAGCGGCG GAGCGCGAGG CGCGCGAGAA AGGGCACTTG GAACCCACCG AGCTGCTGAT GAACCGGGCT TACTTGCAGA GCATTACCCC TCAGGGGTAC TCTGACTCGG AGGAGAGGGA GAGTATGCCG AGGGATGGCG AGAGCGAGAA GGAGCACGAG AAAGAAGGCG AGGATGGCTA CGGGAAGCTG GGCAGACAGG ATGGCGACGA GGAGTTCGAG GAGGAAGAGG AAGAAAGTGA AAATAAAAGT ATGGATACGG ATCCCGAAAC GATACGAGAT GAAGAAGAGA CTGGAGATCA CTCCATGGAC GATAGTTCGG AGGATGGGAA AATGGAAACC AAATCAGACC ACGAGGAAGA CAATATGGAA GATGGCATGC TCGAGTACCC ATACGACGTA CCTGATTATG CAACTTATCC TTATGATGTA CCTGATTATG CTAGCTATCC TTATGATGTC CCAGACTACG CATGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT
