pAAVS1-NDi-TRE3G-SV2C(human)
$99.00
In stock
SKU
PL59200
NM_014979.4
100μg liquid plasmid(Instantly):$120
| name | pAAVS1-NDi-TRE3G-SV2C(human) | another name | apAAVS1-NDi-TRE3G-SV2C(human) |
| Insert Size (bp) | 2184bp | Plasmid host | Mammary cell |
| Plasmid uses | Gene knock-in | Fragment type | ORF,CRISPR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | TRE3G | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCGAATT CGCCCTTAGA GCAGAGCCAG GAACCCCTGT AGGGAAGGGG CAGGAGAGCC AGGGGCATGA GATGGTGGAC GAGGAAGGGG GACAGGGAAG CCTGAGCGCC TCTCCTGGGC TTGCCAAGGA CTCAAACCCA GAAGCCCAGA GCAGGGCCTT AGGGAAGCGG GACCCTGCTC TGGGCGGAGG AATATGTCCC AGATAGCACT GGGGACTCTT TAAGGAAAGA AGGATGGAGA AAGAGAAAGG GAGTAGAGGC GGCCACGACC TGGTGAACAC CTAGGACGCA CCATTCTCAC AAAGGGAGTT TTCCACACGG ACACCCCCCT CCTCACCACA GCCCTGCCAG GACGGGGCTG GCTACTGGCC TTATCTCACA GGTAAAACTG ACGCACGGAG GAACAATATA AATTGGGGAC TAGAAAGGTG AAGAGCCAAA GTTAGAACTC AGGACCAACT TATTCTGATT TTGTTTTTCC AAACTGCTTC TCCTCTTGGG AAGTGTAAGG AAGCTGCAGC ACCAGGATCA GTGAAACGCA CCAGACAGCC GCGTCAGAGC AGCTCAGGTT CTGGGAGAGG GTAGCGCAGG GTGGCCACTG AGAACCGGGC AGGTCACGCA TCCCCCCCTT CCCTCCCACC CCCTGCCAAG CTCTCCCTCC CAGGATCCTC TCTGGCTCCA TCGTAAGCAA ACCTTAGAGG TTCTGGCAAG GAGAGAGATG GCTCCAGGAA ATGGGGGTGT GTCACCAGAT AAGGAATCTG CCTAACAGGA GGTGGGGGTT AGACCCAATA TCAGGAGACT AGGAAGGAGG AGGCCTAAGG ATGGGGCTTT TCTGTCACCA ATCCTGTCCC TAGTAAAGCT GATATCTCCA CCCCACAGTG GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC AGTGATAGAG AACGTATAAG CTTTAGGCGT GTACGGTGGG CGCCTATAAA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGCA ATTCCACAAC ACTTTTGTCT TATACCAACT TTCCGTACCA CTTCCTACCC TCGTAAACTT AAGCCACCAT GGAAGACTCT TACAAGGATA GGACTTCACT GATGAAGGGT GCCAAGGACA TTGCCAGAGA GGTGAAGAAA CAAACAGTAA AGAAGGTGAA TCAAGCTGTG GACCGAGCCC AGGATGAATA CACCCAGAGG TCCTACAGTC GGTTCCAAGA TGAAGAAGAT GATGATGACT ACTACCCGGC TGGAGAAACC TATAATGGTG AGGCCAACGA TGACGAAGGC TCAAGTGAAG CCACTGAGGG GCATGATGAA GATGATGAGA TCTATGAGGG GGAGTATCAG GGCATCCCCA GTATGAACCA AGCGAAGGAC AGCATCGTGT CAGTGGGGCA GCCCAAGGGC GATGAGTACA AGGACCGGCG GGAGCTGGAA TCAGAAAGGA GAGCTGACGA GGAAGAGTTA GCCCAGCAGT ATGAGCTGAT AATCCAAGAA TGCGGTCATG GTCGTTTTCA GTGGGCCCTT TTCTTCGTCC TGGGCATGGC TCTTATGGCA GACGGTGTAG AGGTGTTTGT CGTTGGCTTC GTGTTACCCA GTGCTGAGAC AGACCTCTGC ATCCCAAATT CAGGATCTGG ATGGCTAGGC AGCATAGTGT ACCTCGGGAT GATGGTGGGG GCGTTCTTCT GGGGAGGACT GGCAGACAAA GTGGGAAGGA AACAGTCTCT TCTGATTTGC ATGTCTGTCA ACGGATTCTT TGCCTTCCTT TCTTCATTTG TCCAAGGTTA TGGCTTCTTT CTCTTCTGTC GCTTACTTTC TGGATTCGGG ATTGGAGGAG CCATACCCAC TGTGTTCTCG TACTTTGCTG AAGTCCTGGC CCGGGAAAAG CGGGGCGAAC ACTTGAGCTG GCTCTGCATG TTCTGGATGA TCGGTGGCAT CTACGCCTCT GCCATGGCCT GGGCCATCAT CCCGCACTAC GGGTGGAGCT TCAGCATGGG ATCGGCCTAC CAGTTTCACA GTTGGCGTGT GTTTGTCATC GTCTGTGCAC TCCCCTGTGT CTCCTCCGTG GTGGCCCTCA CATTCATGCC TGAAAGCCCA CGATTCTTGT TGGAGGTTGG AAAACATGAT GAAGCTTGGA TGATTCTGAA GTTAATTCAT GACACCAACA TGAGAGCCCG GGGTCAGCCT GAGAAGGTCT TCACGGTAAA CAAAATAAAA ACTCCTAAAC AAATAGATGA GCTGATTGAA ATTGAGAGTG ACACAGGAAC ATGGTATAGG AGGTGTTTTG TTCGGATCCG CACCGAGCTG TACGGAATTT GGTTGACTTT TATGAGATGT TTCAACTACC CAGTCAGGGA TAATACAATA AAGCTTACAA TTGTTTGGTT CACCCTGTCC TTTGGGTACT ATGGATTATC CGTTTGGTTC CCTGATGTCA TTAAACCTCT GCAGTCCGAT GAATATGCAT TGCTAACCAG AAATGTGGAG AGAGATAAAT ATGCAAATTT CACTATTAAC TTTACAATGG AAAATCAGAT TCATACTGGA ATGGAATACG ACAATGGCAG ATTCATAGGG GTCAAGTTCA AATCTGTAAC TTTCAAAGAC TCTGTTTTTA AGTCCTGCAC CTTTGAGGAT GTAACTTCAG TGAACACCTA CTTCAAGAAC TGCACATTTA TTGACACTGT TTTTGACAAC ACAGATTTTG AGCCATATAA ATTCATTGAC AGTGAATTTA AAAACTGCTC GTTTTTTCAC AACAAGACGG GATGTCAGAT TACCTTTGAT GATGACTATA GTGCCTACTG GATTTATTTT GTCAACTTTC TGGGGACATT GGCAGTATTG CCAGGGAACA TTGTGTCTGC TCTGCTGATG GACAGAATTG GGCGCTTAAC AATGCTAGGT GGCTCTATGG TGCTTTCGGG GATCAGCTGT TTCTTCCTTT GGTTCGGCAC CAGTGAATCC ATGATGATAG GCATGCTGTG TCTGTACAAT GGATTGACCA TCTCAGCCTG GAACTCTCTT GACGTGGTCA CTGTGGAACT GTACCCCACA GACCGGAGGG CAACAGGCTT TGGCTTCTTA AATGCGCTAT GCAAGGCAGC AGCCGTCCTG GGAAACTTAA TATTTGGCTC TCTGGTCAGC ATCACCAAAT CAATCCCCAT CCTGCTGGCT TCTACTGTGC TCGTGTGTGG AGGACTCGTT GGGCTGTGCC TGCCTGACAC ACGAACCCAG GTTCTGATGT AAGCGGCCGC AATCGATCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGACGGCCG CATAACTTCG TATAATGTAT GCTATACGAA GTTATAAGCT TGGGCTGCAG GTCGAGGGAT CTCCATAAGA GAAGAGGGAC AGCTATGACT GGGAGTAGTC AGGAGAGGAG GAAAAATCTG GCTAGTAAAA CATGTAAGGA AAATTTTAGG GATGTTAAAG AAAAAAATAA CACAAAACAA AATATAAAAA AAATCTAACC TCAAGTCAAG GCTTTTCTAT GGAATAAGGA ATGGACAGCA GGGGGCTGTT TCATATACTG ATGACCTCTT TATAGCCAAC CTTTGTTCAT GGCAGCCAGC ATATGGGCAT ATGTTGCCAA ACTCTAAACC AAATACTCAT TCTGATGTTT TAAATGATTT GCCCTCCCAT ATGTCCTTCC GAGTGAGAGA CACAAAAAAT TCCAACACAC TATTGCAATG AAAATAAATT TCCTTTATTA GCCAGAAGTC AGATGCTCAA GGGGCTTCAT GATGTCCCCA TAATTTTTGG CAGAGGGAAA AAGATCTCAG TGGTATTTGT GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGAG TGAATTCACG CGTTTAGCCT GGCAGCATAT CGAGATCGAA GTCATCGAGA GCGTCAGCAG GGAGCATATC CAGATCAAAA TCATCCAGGG CATCGGCGGG CAGCATGTCC AGGTCGAAGT CGTCCAGGGC GTCGGTGGGT CCGCCGCTCT CGCACTTCAG CTGCTTCTCG AGGCCGCAGA TGATCAGTTC CAGGCCGAAC AGGAAGGCGG GCTCGGCGCC CTGTCTGTCG AACAGCTCGA TGGCCTGCTT CAGCAGGGGG GGCATGCTGT CGGTGGTGGG TGTCTCTCTT TCCTCTTTGG CGACCTGGTG TTCCTGTTCT TCCAGCACGC AGCCCAGGGT GAAGTGGCCC ACGGCGCTCA GGGCGTACAG GGCGTTTTCC AGGCTGAAGC CCTGCTGGCA CAGGAAGGCC AGCTGGTTTT CCAGTGTCTC GTACTGCTTC TCGGTGGGTC TGGTGCCCAG GTGCACTTTG GCGCCGTCCC GGTGGCTCAG CAGGGCGCAT CTGTAGCTCT TGGCGTTGTT CCGCAGGAAG TCCTGCCAGC TCTCGCCTTC CAGAGGGCAG CTGTGGGTGT GGTGCCGGTC CAGCATCTCG ATGGGCAGGG CGTCCAGCAG GGCCCGCTTG TTCTTCACGT GCCAGTACAG GGTGGGCTGT TCCACGCCCA GTTTCTGGGC CAGCTTCCGG GTGGTCAGGC CCTCGATGCC CACGCCGTTC AGCAGTTCCA GGGCGCTGTT GATCACTTTG CTCTTGTCCA GCCGGCTCAT GTCGACATTC TTTGCCAAAA TGATGAGACA GCACAATAAC CAGCACGTTG CCCAGGAGCT GTAGGAAAAA GAAGAAGGCA TGAACATGGT TAGCAGAGGC TCTAGAGCCG CCGGTCACAC GCCAGAAGCC GAACCCCGCC CTGCCCCGTC CCCCCCGAAG GCAGCCGTCC CCCCGCGGAC AGCCCCGAGG CTGGAGAGGG AGAAGGGGAC GGCGGCGCGG CGACGCACGA AGGCCCTCCC CGCCCATTTC CTTCCTGCCG GCGCCGCACC GCTTCGCCCC GCGCCCGCTA GAGGGGGTGC GGCGGCGCCT CCCAGATTTC GGCTCCGCAC AGATTTGGGA CAAAGGAAGT CCCTGCGCCC TCTCGCACGA TTACCATAAA AGGCAATGGC TGCGGCTCGC CGCGCCTCGA CAGCCGCCGG CGCTCCGGGG GCCGCCGCGC CCCTCCCCCG AGCCCTCCCC GGCCCGAGGC GGCCCCGCCC CGCCCGGCAC CCCCACCTGC CGCCACCCCC CGCCCGGCAC GGCGAGCCCC GCGCCACGCC CCGTACGGAG CCCCGCACCC GAAGCCGGGC CGTGCTCAGC AACTCGGGGA GGGGGGTGCA GGGGGGGTTG CAGCCCGACC GACGCGCCCA CACCCCCTGC TCACCCCCCC ACGCACACAC CCCGCACGCA GCCTTTGTTC CCCTCGCAGC CCCCCCCGCA CCGCGGGGCA CCGCCCCCGG CCGCGCTCCC CTCGCGCACA CTGCGGAGCG CACAAAGCCC CGCGCCGCGC CCGCAGCGCT CACAGCCGCC GGGCAGCGCG GAGCCGCACG CGGCGCTCCC CACGCACACA CACACGCACG CACCCCCCGA GCCGCTCCCC CCGCACAAAG GGCCCTCCCG GAGCCCCTCA AGGCTTTCAC GCAGCCACAG AAAAGAAACA AGCCGTCATT AAACCAAGCG CTAATTACAG CCCGGAGGAG AAGGGCCGTC CCGCCCGCTC ACCTGTGGGA GTAACGCGGT CAGTCAGAGC CGGGGCGGGC GGCGCGAGGC GGCGGCGGAG CGGGGCACGG GGCGAAGGCA GCGTCGCAGC GACTCCCCGC CCGCCGCGCG CTTCGCTTTT TATAGGGCCG CCGCCGCCGC CGCCTCGCCA TAAAAGGAAA CTTTCGGAGC GCGCCGCTCT GATTGGCTGC CGCCGCACCT CTCCGCCTCG CCCCGCCCCG CCCCTCGCCC CGCCCCGCCC CGCCTGGCGC GCGCCCCCCC CCCCCCCCCG CCCCCATCGC TGCACAAAAT AATTAAAAAA TAAATAAATA CAAAATTGGG GGTGGGGAGG GGGGGGAGAT GGGGAGAGTG AAGCAGAACG TGGGGCTCAC CTCGACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA GGCGGGCCAT TTACCGTAAG TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT GACCCCGTAA TTGATTACTA TTAATAACTA GTCAATAATC AATGTCGAGA CCCATAGAGC CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC CTCCCCCTTG CTGTCCTGCC CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA ATGCGATGCA ATTTCCTCAT TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT CAAGGAAGGC ACGGGGGAGG GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA GGCTGATCAG CGGGTTTAAA CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGGT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC ATCGCCGTCG GGCATGCGCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCCTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATGGCCGAT CCCATGGTGG CGGTACCCTC GAGCCTAGGG CCGGGATTCT CCTCCACGTC ACCGCATGTT AGAAGACTTC CTCTGCCCTC TCCGCTGCCA GATCTCTCGA GGCCCTGTGG GAGGAAGAGA AGAGGTCAGA AGCTTATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT GCCCCACTGT GGGGTGGAGG GGACAGATAA AAGTACCCAG AACCAGAGCC ACATTAACCG GCCCTGGGAA TATAAGGTGG TCCCAGCTCG GGGACACAGG ATCCCTGGAG GCAGCAAACA TGCTGTCCTG AAGTGGACAT AGGGGCCCGG GTTGGAGGAA GAAGACTAGC TGAGCTCTCG GACCCCTGGA AGATGCCATG ACAGGGGGCT GGAAGAGCTA GCACAGACTA GAGAGGTAAG GGGGGTAGGG GAGCTGCCCA AATGAAAGGA GTGAGAGGTG ACCCGAATCC ACAGGAGAAC GGGGTGTCCA GGCAAAGAAA GCAAGAGGAT GGAGAGGTGG CTAAAGCCAG GGAGACGGGG TACTTTGGGG TTGTCCAGAA AAACGGTGAT GATGCAGGCC TACAAGAAGG GGAGGCGGGA CGCAAGGGAG ACATCCGTCG GAGAAGGCCA TCCTAAGAAA CGAGAGATGG CACAGGCCCC AGAAGGAGAA GGAAAAGGGA ACCCAGCGAG TGAAGACGGC ATGGGGTTGG GTGAGGGAGG AGAGATGCCC GGAGAGGACC CAGACACGGG GAGGATCCGC TCAGAGGACA TCACGTGGTG CAGCGCCGAG AAGGAAGTGC TCCGGAAAGA GCATCCTTGG GCAGCAACAC AGCAGAGAGC AAGGGGAAGA GGGAGTGGAG GAAGACGGAA CCTGAAGGAG GCGGCAGGGA AGGATCTGGG CCAGCCGTAG AGGTGACCCA GGCCACAAGC TGCAGACAGA AAGCGGCACA GGCCCAGGGG AGAGAATGCT GGTCAGAGAA AGCAAAGGGC GAATTCAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAA
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