pX458-EGR1-PLK1(human)-sgRNA1-EnCMV-Cas9-T2A-EGFP
$99.00
In stock
SKU
PL59198
100μg liquid plasmid(Instantly):$120
| name | pX458-EGR1-PLK1(human)-sgRNA1-EnCMV-Cas9-T2A-EGFP | another name | apX458-EGR1-PLK1(human)-sgRNA1-EnCMV-Cas9-T2A-EGFP |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | EGR1 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGTTCGCTC TCACGGTCCC TGAGGTGGGC GGGCGGGCCC TGGATGACAG CGATAGAACC CCGGCCCGAC TCGCCCTCGC CCCCGCTCTG GGTCTGGGCT TCCCCAGCCT AGTTCACGCC TAGGAGCCGC CTGAGCAGCC GCGCGCCCAG CGCCACACGC CACGAGCCCT CCCCGCCTGG GCGTCCCCGG ATCCCGCGAG CGCTCGGGCT CCCGGCTTGG AACCAGGGAG GAGGGAGGGA GCGAGGGAGC AACCAGCTGC GGACCCGGAA ATGCCATATA AGAAGCAGGA AGGATCCCCC GCCGGAACAA CCCTTATTTG GGCAGCACCT TATTTGGAGT GGCCCGATAT GGCCCGGCCG CTTCCGGCTC TGGGAGGAGG GAAGAAGGCG GAGGGAGGGG CAACGCGGGA ACTCCGGAGC TGCGCGGGTC CCGGAGGCCC CGGCGGCGGC TAGAGCTCTA GGCTTCCCCG AAGCCTGGGC GCCTGGGATG CGGGCGCGGG CGCGGGCCCT AGGGTGCAGG ATGGAGGTGC CGGGCGCTGT CGGATGGGGG GCTTCACGTC ACTCCGGGTC CTCCCGGCCG GTCCTGCCAT ATTAGGGCTT CCTGCTTCCC ATATATGGCC ATGTACGTCA CGACGGAGGC GGACCCGTGC CGTTCCAGAC CCTTCAAATA GAGGCGGATC CGGGGAGTCG CGAGAGATCC AGCGAAACAC CGCGTGGAAT CCTACGACGT GCGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTTT TAGCGCGTGC GCCAATTCTG CAGACAAATG GCTCTAGAGG TACCCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTGTGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTCGAGGTGA GCCCCACGTT CTGCTTCACT CTCCCCATCT CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT TTAATTATTT TGTGCAGCGA TGGGGGCGGG GGGGGGGGGG GGGCGCGCGC CAGGCGGGGC GGGGCGGGGC GAGGGGCGGG GCGGGGCGAG GCGGAGAGGT GCGGCGGCAG CCAATCAGAG CGGCGCGCTC CGAAAGTTTC CTTTTATGGC GAGGCGGCGG CGGCGGCGGC CCTATAAAAA GCGAAGCGCG CGGCGGGCGG GAGTCGCTGC GACGCTGCCT TCGCCCCGTG CCCCGCTCCG CCGCCGCCTC GCGCCGCCCG CCCCGGCTCT GACTGACCGC GTTACTCCCA CAGGTGAGCG GGCGGGACGG CCCTTCTCCT CCGGGCTGTA ATTAGCTGAG CAAGAGGTAA GGGTTTAAGG GATGGTTGGT TGGTGGGGTA TTAATGTTTA ATTACCTGGA GCACCTGCCT GAAATCACTT TTTTTCAGGT TGGACCGGTG CCACCATGGA CTATAAGGAC CACGACGGAG ACTACAAGGA TCATGATATT GATTACAAAG ACGATGACGA TAAGATGGCC CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGACAA GAAGTACAGC ATCGGCCTGG ACATCGGCAC CAACTCTGTG GGCTGGGCCG TGATCACCGA CGAGTACAAG GTGCCCAGCA AGAAATTCAA GGTGCTGGGC AACACCGACC GGCACAGCAT CAAGAAGAAC CTGATCGGAG CCCTGCTGTT CGACAGCGGC GAAACAGCCG AGGCCACCCG GCTGAAGAGA ACCGCCAGAA GAAGATACAC CAGACGGAAG AACCGGATCT GCTATCTGCA AGAGATCTTC AGCAACGAGA TGGCCAAGGT GGACGACAGC TTCTTCCACA GACTGGAAGA GTCCTTCCTG GTGGAAGAGG ATAAGAAGCA CGAGCGGCAC CCCATCTTCG GCAACATCGT GGACGAGGTG GCCTACCACG AGAAGTACCC CACCATCTAC CACCTGAGAA AGAAACTGGT GGACAGCACC GACAAGGCCG ACCTGCGGCT GATCTATCTG GCCCTGGCCC ACATGATCAA GTTCCGGGGC CACTTCCTGA TCGAGGGCGA CCTGAACCCC GACAACAGCG ACGTGGACAA GCTGTTCATC CAGCTGGTGC AGACCTACAA CCAGCTGTTC GAGGAAAACC CCATCAACGC CAGCGGCGTG GACGCCAAGG CCATCCTGTC TGCCAGACTG AGCAAGAGCA GACGGCTGGA AAATCTGATC GCCCAGCTGC CCGGCGAGAA GAAGAATGGC CTGTTCGGAA ACCTGATTGC CCTGAGCCTG GGCCTGACCC CCAACTTCAA GAGCAACTTC GACCTGGCCG AGGATGCCAA ACTGCAGCTG AGCAAGGACA CCTACGACGA CGACCTGGAC AACCTGCTGG CCCAGATCGG CGACCAGTAC GCCGACCTGT TTCTGGCCGC CAAGAACCTG TCCGACGCCA TCCTGCTGAG CGACATCCTG AGAGTGAACA CCGAGATCAC CAAGGCCCCC CTGAGCGCCT CTATGATCAA GAGATACGAC GAGCACCACC AGGACCTGAC CCTGCTGAAA GCTCTCGTGC GGCAGCAGCT GCCTGAGAAG TACAAAGAGA TTTTCTTCGA CCAGAGCAAG AACGGCTACG CCGGCTACAT TGACGGCGGA GCCAGCCAGG AAGAGTTCTA CAAGTTCATC AAGCCCATCC TGGAAAAGAT GGACGGCACC GAGGAACTGC TCGTGAAGCT GAACAGAGAG GACCTGCTGC GGAAGCAGCG GACCTTCGAC AACGGCAGCA TCCCCCACCA GATCCACCTG GGAGAGCTGC ACGCCATTCT GCGGCGGCAG GAAGATTTTT ACCCATTCCT GAAGGACAAC CGGGAAAAGA TCGAGAAGAT CCTGACCTTC CGCATCCCCT ACTACGTGGG CCCTCTGGCC AGGGGAAACA GCAGATTCGC CTGGATGACC AGAAAGAGCG AGGAAACCAT CACCCCCTGG AACTTCGAGG AAGTGGTGGA CAAGGGCGCT TCCGCCCAGA GCTTCATCGA GCGGATGACC AACTTCGATA AGAACCTGCC CAACGAGAAG GTGCTGCCCA AGCACAGCCT GCTGTACGAG TACTTCACCG TGTATAACGA GCTGACCAAA GTGAAATACG TGACCGAGGG AATGAGAAAG CCCGCCTTCC TGAGCGGCGA GCAGAAAAAG GCCATCGTGG ACCTGCTGTT CAAGACCAAC CGGAAAGTGA CCGTGAAGCA GCTGAAAGAG GACTACTTCA AGAAAATCGA GTGCTTCGAC TCCGTGGAAA TCTCCGGCGT GGAAGATCGG TTCAACGCCT CCCTGGGCAC ATACCACGAT CTGCTGAAAA TTATCAAGGA CAAGGACTTC CTGGACAATG AGGAAAACGA GGACATTCTG GAAGATATCG TGCTGACCCT GACACTGTTT GAGGACAGAG AGATGATCGA GGAACGGCTG AAAACCTATG CCCACCTGTT CGACGACAAA GTGATGAAGC AGCTGAAGCG GCGGAGATAC ACCGGCTGGG GCAGGCTGAG CCGGAAGCTG ATCAACGGCA TCCGGGACAA GCAGTCCGGC AAGACAATCC TGGATTTCCT GAAGTCCGAC GGCTTCGCCA ACAGAAACTT CATGCAGCTG ATCCACGACG ACAGCCTGAC CTTTAAAGAG GACATCCAGA AAGCCCAGGT GTCCGGCCAG GGCGATAGCC TGCACGAGCA CATTGCCAAT CTGGCCGGCA GCCCCGCCAT TAAGAAGGGC ATCCTGCAGA CAGTGAAGGT GGTGGACGAG CTCGTGAAAG TGATGGGCCG GCACAAGCCC GAGAACATCG TGATCGAAAT GGCCAGAGAG AACCAGACCA CCCAGAAGGG ACAGAAGAAC AGCCGCGAGA GAATGAAGCG GATCGAAGAG GGCATCAAAG AGCTGGGCAG CCAGATCCTG AAAGAACACC CCGTGGAAAA CACCCAGCTG CAGAACGAGA AGCTGTACCT GTACTACCTG CAGAATGGGC GGGATATGTA CGTGGACCAG GAACTGGACA TCAACCGGCT GTCCGACTAC GATGTGGACC ATATCGTGCC TCAGAGCTTT CTGAAGGACG ACTCCATCGA CAACAAGGTG CTGACCAGAA GCGACAAGAA CCGGGGCAAG AGCGACAACG TGCCCTCCGA AGAGGTCGTG AAGAAGATGA AGAACTACTG GCGGCAGCTG CTGAACGCCA AGCTGATTAC CCAGAGAAAG TTCGACAATC TGACCAAGGC CGAGAGAGGC GGCCTGAGCG AACTGGATAA GGCCGGCTTC ATCAAGAGAC AGCTGGTGGA AACCCGGCAG ATCACAAAGC ACGTGGCACA GATCCTGGAC TCCCGGATGA ACACTAAGTA CGACGAGAAT GACAAGCTGA TCCGGGAAGT GAAAGTGATC ACCCTGAAGT CCAAGCTGGT GTCCGATTTC CGGAAGGATT TCCAGTTTTA CAAAGTGCGC GAGATCAACA ACTACCACCA CGCCCACGAC GCCTACCTGA ACGCCGTCGT GGGAACCGCC CTGATCAAAA AGTACCCTAA GCTGGAAAGC GAGTTCGTGT ACGGCGACTA CAAGGTGTAC GACGTGCGGA AGATGATCGC CAAGAGCGAG CAGGAAATCG GCAAGGCTAC CGCCAAGTAC TTCTTCTACA GCAACATCAT GAACTTTTTC AAGACCGAGA TTACCCTGGC CAACGGCGAG ATCCGGAAGC GGCCTCTGAT CGAGACAAAC GGCGAAACCG GGGAGATCGT GTGGGATAAG GGCCGGGATT TTGCCACCGT GCGGAAAGTG CTGAGCATGC CCCAAGTGAA TATCGTGAAA AAGACCGAGG TGCAGACAGG CGGCTTCAGC AAAGAGTCTA TCCTGCCCAA GAGGAACAGC GATAAGCTGA TCGCCAGAAA GAAGGACTGG GACCCTAAGA AGTACGGCGG CTTCGACAGC CCCACCGTGG CCTATTCTGT GCTGGTGGTG GCCAAAGTGG AAAAGGGCAA GTCCAAGAAA CTGAAGAGTG TGAAAGAGCT GCTGGGGATC ACCATCATGG AAAGAAGCAG CTTCGAGAAG AATCCCATCG ACTTTCTGGA AGCCAAGGGC TACAAAGAAG TGAAAAAGGA CCTGATCATC AAGCTGCCTA AGTACTCCCT GTTCGAGCTG GAAAACGGCC GGAAGAGAAT GCTGGCCTCT GCCGGCGAAC TGCAGAAGGG AAACGAACTG GCCCTGCCCT CCAAATATGT GAACTTCCTG TACCTGGCCA GCCACTATGA GAAGCTGAAG GGCTCCCCCG AGGATAATGA GCAGAAACAG CTGTTTGTGG AACAGCACAA GCACTACCTG GACGAGATCA TCGAGCAGAT CAGCGAGTTC TCCAAGAGAG TGATCCTGGC CGACGCTAAT CTGGACAAAG TGCTGTCCGC CTACAACAAG CACCGGGATA AGCCCATCAG AGAGCAGGCC GAGAATATCA TCCACCTGTT TACCCTGACC AATCTGGGAG CCCCTGCCGC CTTCAAGTAC TTTGACACCA CCATCGACCG GAAGAGGTAC ACCAGCACCA AAGAGGTGCT GGACGCCACC CTGATCCACC AGAGCATCAC CGGCCTGTAC GAGACACGGA TCGACCTGTC TCAGCTGGGA GGCGACAAAA GGCCGGCGGC CACGAAAAAG GCCGGCCAGG CAAAAAAGAA AAAGGAATTC GGCAGTGGAG AGGGCAGAGG AAGTCTGCTA ACATGCGGTG ACGTCGAGGA GAATCCTGGC CCAGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGAA TTCTAACTAG AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGAGAA TAGCAGGCAT GCTGGGGAGC GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCTGC CTGCAGGGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGTCAAA GCAACCATAG TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGA AGCCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGT
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