pAAV-EF1a-RfxCasR13d-P2A-GFP-U6-gRNA1-gRNA2
$113.00
In stock
SKU
PL59196
100μg liquid plasmid(Instantly):$134
| name | pAAV-EF1a-RfxCasR13d-P2A-GFP-U6-gRNA1-gRNA2 | another name | apAAV-EF1a-RfxCasR13d-P2A-GFP-U6-gRNA1-gRNA2 |
| Insert Size (bp) | Plasmid host | Mammary cell,Adeno-associated virus | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | EF1a,U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT AGGGGTTCCT GCGGCCGCAC GCGTAAGCTT TGCAAAGATG GATAAAGTTT TAAACAGAGA GGAATCTTTG CAGCTAATGG ACCTTCTAGG TCTTGAAAGG AGTGGGAATT GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GTACGACCGG TGCCACCATG CCTAAAAAGA AAAGAAAGGT GGGTTCTGGT ATCGAAAAAA AAAAGTCCTT CGCCAAGGGC ATGGGCGTGA AGTCCACACT CGTGTCCGGC TCCAAAGTGT ACATGACAAC CTTCGCCGAA GGCAGCGACG CCAGGCTGGA AAAGATCGTG GAGGGCGACA GCATCAGGAG CGTGAATGAG GGCGAGGCCT TCAGCGCTGA AATGGCCGAT AAAAACGCCG GCTATAAGAT CGGCAACGCC AAATTCAGCC ATCCTAAGGG CTACGCCGTG GTGGCTAACA ACCCTCTGTA TACAGGACCC GTCCAGCAGG ATATGCTCGG CCTGAAGGAA ACTCTGGAAA AGAGGTACTT CGGCGAGAGC GCTGATGGCA ATGACAATAT TTGTATCCAG GTGATCCATA ACATCCTGGA CATTGAAAAA ATCCTCGCCG AATACATTAC CAACGCCGCC TACGCCGTCA ACAATATCTC CGGCCTGGAT AAGGACATTA TTGGATTCGG CAAGTTCTCC ACAGTGTATA CCTACGACGA ATTCAAAGAC CCCGAGCACC ATAGGGCCGC TTTCAACAAT AACGATAAGC TCATCAACGC CATCAAGGCC CAGTATGACG AGTTCGACAA CTTCCTCGAT AACCCCAGAC TCGGCTATTT CGGCCAGGCC TTTTTCAGCA AGGAGGGCAG AAATTACATC ATCAATTACG GCAACGAATG CTATGACATT CTGGCCCTCC TGAGCGGACT GAGGCACTGG GTGGTCCATA ACAACGAAGA AGAGTCCAGG ATCTCCAGGA CCTGGCTCTA CAACCTCGAT AAGAACCTCG ACAACGAATA CATCTCCACC CTCAACTACC TCTACGACAG GATCACCAAT GAGCTGACCA ACTCCTTCTC CAAGAACTCC GCCGCCAACG TGAACTATAT TGCCGAAACT CTGGGAATCA ACCCTGCCGA ATTCGCCGAA CAATATTTCA GATTCAGCAT TATGAAAGAG CAGAAAAACC TCGGATTCAA TATCACCAAG CTCAGGGAAG TGATGCTGGA CAGGAAGGAT ATGTCCGAGA TCAGGAAAAA TCATAAGGTG TTCGACTCCA TCAGGACCAA GGTCTACACC ATGATGGACT TTGTGATTTA TAGGTATTAC ATCGAAGAGG ATGCCAAGGT GGCTGCCGCC AATAAGTCCC TCCCCGATAA TGAGAAGTCC CTGAGCGAGA AGGATATCTT TGTGATTAAC CTGAGGGGCT CCTTCAACGA CGACCAGAAG GATGCCCTCT ACTACGATGA AGCTAATAGA ATTTGGAGAA AGCTCGAAAA TATCATGCAC AACATCAAGG AATTTAGGGG AAACAAGACA AGAGAGTATA AGAAGAAGGA CGCCCCTAGA CTGCCCAGAA TCCTGCCCGC TGGCCGTGAT GTTTCCGCCT TCAGCAAACT CATGTATGCC CTGACCATGT TCCTGGATGG CAAGGAGATC AACGACCTCC TGACCACCCT GATTAATAAA TTCGATAACA TCCAGAGCTT CCTGAAGGTG ATGCCTCTCA TCGGAGTCAA CGCTAAGTTC GTGGAGGAAT ACGCCTTTTT CAAAGACTCC GCCAAGATCG CCGATGAGCT GAGGCTGATC AAGTCCTTCG CTAGAATGGG AGAACCTATT GCCGATGCCA GGAGGGCCAT GTATATCGAC GCCATCCGTA TTTTAGGAAC CAACCTGTCC TATGATGAGC TCAAGGCCCT CGCCGACACC TTTTCCCTGG ACGAGAACGG AAACAAGCTC AAGAAAGGCA AGCACGGCAT GAGAAATTTC ATTATTAATA ACGTGATCAG CAATAAAAGG TTCCACTACC TGATCAGATA CGGTGATCCT GCCCACCTCC ATGAGATCGC CAAAAACGAG GCCGTGGTGA AGTTCGTGCT CGGCAGGATC GCTGACATCC AGAAAAAACA GGGCCAGAAC GGCAAGAACC AGATCGACAG GTACTACGAA ACTTGTATCG GAAAGGATAA GGGCAAGAGC GTGAGCGAAA AGGTGGACGC TCTCACAAAG ATCATCACCG GAATGAACTA CGACCAATTC GACAAGAAAA GGAGCGTCAT TGAGGACACC GGCAGGGAAA ACGCCGAGAG GGAGAAGTTT AAAAAGATCA TCAGCCTGTA CCTCACCGTG ATCTACCACA TCCTCAAGAA TATTGTCAAT ATCAACGCCA GGTACGTCAT CGGATTCCAT TGCGTCGAGC GTGATGCTCA ACTGTACAAG GAGAAAGGCT ACGACATCAA TCTCAAGAAA CTGGAAGAGA AGGGATTCAG CTCCGTCACC AAGCTCTGCG CTGGCATTGA TGAAACTGCC CCCGATAAGA GAAAGGACGT GGAAAAGGAG ATGGCTGAAA GAGCCAAGGA GAGCATTGAC AGCCTCGAGA GCGCCAACCC CAAGCTGTAT GCCAATTACA TCAAATACAG CGACGAGAAG AAAGCCGAGG AGTTCACCAG GCAGATTAAC AGGGAGAAGG CCAAAACCGC CCTGAACGCC TACCTGAGGA ACACCAAGTG GAATGTGATC ATCAGGGAGG ACCTCCTGAG AATTGACAAC AAGACATGTA CCCTGTTCAG AAACAAGGCC GTCCACCTGG AAGTGGCCAG GTATGTCCAC GCCTATATCA ACGACATTGC CGAGGTCAAT TCCTACTTCC AACTGTACCA TTACATCATG CAGAGAATTA TCATGAATGA GAGGTACGAG AAAAGCAGCG GAAAGGTGTC CGAGTACTTC GACGCTGTGA ATGACGAGAA GAAGTACAAC GATAGGCTCC TGAAACTGCT GTGTGTGCCT TTCGGCTACT GTATCCCCAG GTTTAAGAAC CTGAGCATCG AGGCCCTGTT CGATAGGAAC GAGGCCGCCA AGTTCGACAA GGAGAAAAAG AAGGTGTCCG GCAATTCCGG TTCTGGTTAC CCATACGACG TACCAGATTA CGCTCCCAAG AAGAAGCGCA AGGTGGGATC CGGCGCAACA AACTTCTCTC TGCTGAAACA AGCCGGAGAT GTCGAAGAGA ATCCTGGACC GATGGTGTCT AAGGGCGAAG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AGGATAAAGA TCCTAAAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CGCGGCCGAG GGCCTATTTC CCATGATTCC TTCATATTTG CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA ATTTGACTGT AAACACAAAG ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC TTGGGTAGTT TGCAGTTTTA AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGAAC CCCTACCAAC TGGTCGGGGT TTGAAACGGG GAAGCTACAC TGTTGTCCAA GCCATCACAA GTAAACCCCT ACCAACTGGT CGGGGTTTGA AACACAGGAG CTGGAGATGC CCCCTGAGGC ACCCAAGTAA ACCCCTACCA ACTGGTCGGG GTTTGAAACT TTTTTTGAAT TCTGATGCGG TGGCGGCCGC CACGTGCGGA CCGAGCGGCC GCAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCTGCCTGC AGGGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAC GTCAAAGCAA CCATAGTACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCTTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTTGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACT CTATCTCGGG CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGTC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA TTTTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
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