pFLP2
$113.00
In stock
SKU
PL59168
100μg liquid plasmid(Instantly):$134
| name | pFLP2 | another name | apFLP2 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATACCTC TCAGGCGCCG CTGGTGCCGC TGGTTGGACG CCAAGGGTGA ATCCGCCTCG ATACCCTGAT TACTCGCTTC CTGCGCCCTC TCAGGCGGCG ATAGGGGACT GGTAAAACGG GGATTGCCCA GACGCCTCCC CCGCCCCTTC AGGGGCACAA ATGCGGCCCC AACGGGGCCA CGTAGTGGTG CGTTTTTTGC GTTTCCACCC TTTTCTTCCT TTTCCCTTTT AAACCTTTTA GGACGTCTAC AGGCCACGTA ATCCGTGGCC TGTAGAGTTT AAAAAGGGAC GGATTTGTTG CCATTAAGGG ACGGATTTGT TGTTAAGAAG GGACGGATTT GTTGTTGTAA AGGGACGGAT TTGTTGTATT GTGGGACGCA GATACAGTGT CCCCTTATAC ACAAGGAATG TCGAACGTGG CCTCACCCCC AATGGTTTAC AAAAGCAATG CCCTGGTCGA GGCCGCGTAT CGCCTCAGTG TTCAGGAACA GCGGATCGTT CTGGCCTGTA TTAGCCAGGT GAAGAGGAGC GAGCCTGTCA CCGATGAAGT GATGTATTCA GTGACGGCGG AGGACATAGC GACGATGGCG GGTGTCCCTA TCGAATCTTC CTACAACCAG CTCAAAGAAG CGGCCCTGCG CCTGAAACGG CGGGAAGTCC GGTTAACCCA AGAGCCCAAT GGCAAGGGGA AAAGACCGAG TGTGATGATT ACCGGCTGGG TGCAAACAAT CATCTACCGG GAGGGTGAGG GCCGTGTAGA ACTCAGGTTC ACCAAAGACA TGCTGCCGTA CCTGACGGAA CTCACCAAAC AGTTCACCAA ATACGCCTTG GCTGACGTGG CCAAGATGGA CAGCACCCAC GCGATCAGGC TTTACGAGCT GCTCATGCAA TGGGACAGCA TCGGCCAGCG CGAAATAGAA ATTGACCAGC TGCGAAAGTG GTTTCAACTG GAAGGCCGGT ATCCCTCGAT CAAGGACTTC AAGTTGCGAG TGCTTGATCC AGCCGTGACG CAGATCAACG AGCACAGCCC GCTACAGGTG GAGTGGGCGC AGCGAAAGAC CGGGCGCAAG GTCACACATC TGTTGTTCAG TTTTGGACCG AAGAAGCCCG CCAAGGCGGT GGGTAAGGCC CCAGCGAAGC GCAAGGCCGG GAAGATTTCA GATGCTGAGA TCGCGAAACA GGCTCGCCCT GGTGAGACAT GGGAAGCGGC CCGCGCTCGA CTAACCCAGA TGCCGCTGGA TCTGGCCTAG AGGCCGTGGC CACCACGGCC CGGCCTGCCT TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTTGCAT GCCTGCAGTT CACTTACACC GCTTCTCAAC CCGGTACGCA CCAGAAAATC ATTGATATGG CCATGAATGG CGTTGGATGC CGGGCAACAG CCCGCATTAT GGGCGTTGGC CTCAACACGA TTTTACGTCA CTTAAAAAAC TCAGGCCGCA GTCGGTAACC TCGCGCATAC AGCCGGGCAG TGACGTCATC GTCTGCGCGG AAATGGACGA ACAGTGGGGC TATGTCGGGG CTAAATCGCG CCAGCGCTGG CTGTTTTACG CGTATGACAG TCTCCGGAAG ACGGTTGTTG CGCACGTATT CGGTGAACGC ACTATGGCGA CGCTGGGGCG TCTTATGAGC CTGCTGTCAC CCTTTGACGT GGTGATATGG ATGACGGATG GCTGGCCGCT GTATGAATCC CGCCTGAAGG GAAAGCTGCA CGTAATCAGC AAGCGATATA CGCAGCGAAT TGAGCGGCAT AACCTGAATC TGAGGCAGCA CCTGGCACGG CTGGGACGGA AGTCGCTGTC GTTCTCAAAA TCGGTGGAGC TGCATGACAA AGTCATCGGG CATTATCTGA ACATAAAACA CTATCAATAA GTTGGAGTCA TTACCAAAAG GTTAGGAATA CGGTTAGCCA TTTGCCTGCT TTTATATAGT TCATATGGGA TTCACCTTTA TGTTGATAAG AAATAAAAGA AAATGCCAAT AGGATATCGG CATTTTCTTT TGCGTTTTTA TTTGTTAACT GTTAATTGTC CTTGTTCAAG GATGCTGTCT TTGACAACAG ATGTTTTCTT GCCTTTGATG TTCAGCAGGA AGCTAGGCGC AAACGTTGAT TGTTTGTCTG CGTAGAATCC TCTGTTTGTC ATATAGCTTG TAATCACGAC ATTGTTTCCT TTCGCTTGAG GTACAGCGAA GTGTGAGTAA GTAAAGGTTA CATCGTTAGG ATCAAGATCC ATTTTTAACA CAAGGCCAGT TTTGTTCAGC GGCTTGTATG GGCCAGTTAA AGAATTAGAA ACATAACCAA GCATGTAAAT ATCGTTAGAC GTAATGCCGT CAATCGTCAT TTTTGATCCG CGGGAGTCAG TGAACAGATA CCATTTGCCG TTCATTTTAA AGACGTTCGC GCGTTCAATT TCATCTGTTA CTGTGTTAGA TGCAATCAGC GGTTTCATCA CTTTTTTCAG TGTGTAATCA TCGTTTAGCT CAATCATACC GAGAGCGCCG TTTGCTAACT CAGCCGTGCG TTTTTTATCG CTTTGCAGAA GTTTTTGACT TTCTTGACGG AAGAATGATG TGCTTTTGCC ATAGTATGCT TTGTTAAATA AAGATTCTTC GCCTTGGTAG CCATCTTCAG TTCCAGTGTT TGCTTCAAAT ACTAAGTATT TGTGGCCTTT ATCTTCTACG TAGTGAGGAT CTCTCAGCGT ATGGTTGTCG CCTGAGCTGT AGTTGCCTTC ATCGATGAAC TGCTGTACAT TTTGATACGT TTTTCCGTCA CCGTCAAAGA TTGATTTATA ATCCTCTACA CCGTTGATGT TCAAAGAGCT GTCTGATGCT GATACGTTAA CTTGTGCAGT TGTCAGTGTT TGTTTGCCGT AATGTTTACC GGAGAAATCA GTGTAGAATA AACGGATTTT TCCGTCAGAT GTAAATGTGG CTGAACCTGA CCATTCTTGT GTTTGGTCTT TTAGGATAGA ATCATTTGCA TCGAATTTGT CGCTGTCTTT AAAGACGCGG CCAGCGTTTT TCCAGCTGTC AATAGAAGTT TCGCCGACTT TTTGATAGAA CATGTAAATC GATGTGTCAT CCGCATTTTT AGGATCTCCG GCTAATGCAA AGACGATGTG GTAGCCGTGA TAGTTTGCGA CAGTGCCGTC AGCGTTTTGT AATGGCCAGC TGTCCCAAAC GTCCAGGCCT TTTGCAGAAG AGATATTTTT AATTGTGGAC GAATCGAACT CAGGAACTTG ATATTTTTCA TTTTTTTGCT GTTCAGGGAT TTGCAGCATA TCATGGCGTG TAATATGGGA AATGCCGTAT GTTTCCTTAT ATGGCTTTTG GTTCGTTTCT TTCGCAAACG CTTGAGTTGC GCCTCCTGCC AGCAGTGCGG TAGTAAAGGT TAATACTGTT GCTTGTTTTG CAAACTTTTT GATGTTCATC GTTCATGTCT CCTTTTTTAT GTACTGTGTT AGCGGTCTGC TTCTTCCAGC CCTCCTGTTT GAAGATGGCA AGTTAGTTAC GCACAATAAA AAAAGACCTA AAATATGTAA GGGGTGACGC CAAAGTATAC ACTTTGCCCT TTACACATTT TAGGTCTTGC CTGCTTTATC AGTAACAAAC CCGCGCGATT TACTTTTCGA CCTCATTCTA TTAGACTCTC GTTTGGATTG CAACTGGTCT ATTTTCCTCT TTTGTTTGAT AGAAAATCAT AAAAGGATTT GCAGACTACG GGCCTAAAGA ACTAAAAAAT CTATCTGTTT CTTTTCATTC TCTGTATTTT TTATAGTTTC TGTTGCATGG GCATAAAGTT GCCTTTTTAA TCACAATTCA GAAAATATCA TAATATCTCA TTTCACTAAA TAATAGTGAA CGGCAGGTAT ATGTGATGGG TTAAAAAGGA TCGATCCTCT AGAGGATCCC CCTAGAGAAT AGGAACTTCG GAATAGGAAC TTCAAAGCGT TTCCGAAAAC GAGCGCTTCC GAAAATGCAA CGCGAGCTGC GCACATACAG CTCACTGTTC ACGTCGCACC TATATCTGCG TGTTGCCTGT ATATATATAT ACATGAGAAG AACGGCATAG TGCGTGTTTA TGCTTAAATG CGTACTTATA TGCGTCTATT TATGTAGGAT GAAAGGTAGT CTAGTACCTC CTGTGATATT ATCCCATTCC ATGCGGGGTA TCGTATGCTT CCTTCAGCAC TACCCTTTAG CTGTTCTATA TGCTGCCACT CCTCAATTGG ATTAGTCTCA TCCTTCAATG CTATCATTTC CTTTGATATT GGATCATATG CATAGTACCG AGAAACTAGT GCGAAGTAGT GATCAGGTAT TGCTGTTATC TGATGAGTAT ACGTTGTCCT GGCCACGGCA GAAGCACGCT TATCGCTCCA ATTTCCCACA ACATTAGTCA ACTCCGTTAG GCCCTTCATT GAAAGAAATG AGGTCATCAA ATGTCTTCCA ATGTGAGATT TTGGGCCATT TTTTATAGCA AAGATTGAAT AAGGCGCATT TTTCTTCAAA GCTTTATTGT ACGATCTGAC TAAGTTATCT TTTAATAATT GGTATTCCTG TTTATTGCTT GAAGAATTGC CGGTCCTATT TACTCGTTTT AGGACTGGTT CAGAATTCCT CAAAAATTCA TCCAAATATA CAAGTGGATC GATCCTACCC CTTGCGCTAA AGAAGTATAT GTGCCTACTA ACGCTTGTCT TTGTCTCTGT CACTAAACAC TGGATTATTA CTCCCAGATA CTTATTTTGG ACTAATTTAA ATGATTTCGG ATCAACGTTC TTAATATCGC TGAATCTTCC ACAATTGATG AAAGTAGCTA GGAAGAGGAA TTGGTATAAA GTTTTTGTTT TTGTAAATCT CGAAGTATAC TCAAACGAAT TTAGTATTTT CTCAGTGATC TCCCAGATGC TTTCACCCTC ACTTAGAAGT GCTTTAAGCA TTTTTTTACT GTGGCTATTT CCCTTATCTG CTTCTTCCGA TGATTCGAAC TGTAATTGCA AACTACTTAC AATATCAGTG ATATCAGATT GATGTTTTTG TCCATAGTAA GGAATAATTG TAAATTCCCA AGCAGGAATC AATTTCTTTA ATGAGGCTTC CAGAATTGTT GCTTTTTGCG TCTTGTATTT AAACTGGAGT GATTTATTGA CAATATCGAA ACTCAGCGAA TTGCTTATGA TAGTATTATA GCTCATGAAT GTGGCTCTCT TGATTGCTGT TCCGTTATGT GTAATCATCC AACATAAATA GGTTAGTTCA GCAGCACATA ATGCTATTTT CTCACCTGAA GGTCTTTCAA ACCTTTCCAC AAACTGACGA ACAAGCACCT TAGGTGGTGT TTTACATAAT ATACCAAATT GTGGCATACA ACCTCCTTAG TACATGCAAC CATTATCACC GCCAGAGGTA AAATAGTCAA CACGCACGGT GTTAGATATT TATCCCTTGC GGTGATAGAT TTAACGTATG AGCACAAAAA AGAAACCATT AACACAAGAG CAGCTTGAGG ACGCACGTCG CCTTAAAGCA ATTTATGAAA AAAAGAAAAA TGAACTTGGC TTATCCCAGG AATCTGTCGC AGACAAGATG GGGATGGGGC AGTCAGGCGT TGGTGCTTTA TTTAATGGCA TCAATGCATT AAATGCTTAT AACGCCGCAT TGCTTACAAA AATTCTCAAA GTTAGCGTTG AAGAATTTAG CCCTTCAATC GCCAGAGAAA TCTACGAGAT GTATGAAGCG GTTAGTATGC AGCCGTCACT TAGAAGTGAG TATGAGTACC CTGTTTTTTC TCATGTTCAG GCAGGGATGT TCTCACCTAA GCTTAGAACC TTTACCAAAG GTGATGCGGA GAGATGGGTA AGCACAACCA AAAAAGCCAG TGATTCTGCA TTCTGGCTTG AGGTTGAAGG TAATTCCATG ACCGCACCAA CAGGCTCCAA GCCAAGCTTT CCTGACGGAA TGTTAATTCT CGTTGACCCT GAGCAGGCTG TTGAGCCAGG TGATTTCTGC ATAGCCAGAC TTGGGGGTGA TGAGTTTACC TTCAAGAAAC TGATCAGGGA TAGCGGTCAG GTGTTTTTAC AACCACTAAA CCCACAGTAC CCAATGATCC CATGCAATGA GAGTTGTTCC GTTGTGGGGA AAGTTATCGC TAGTCAGTGG CCTGAAGAGA CGTTTGGCTG ATCGGCAAGG TGTTCTGGTC GGCGCATAGC TGATAACAAT TGAGCAAGAA TCTTCATCGA ATTAGGGGAA TTTTCACTCC CCTCAGAACA TAACATAGTA AATGGATTGA ATTATGAAGA ATGGTTTTTA TGCGACTTAC CGCAGCAAAA ATAAAGGGAA AGATAAGCGC TCAATAAACC TGTCTGTTTT CCTTAATTCT CTGCTGGCTG ATAATCATCA CCTGCAGGTT GGCTCCAATT ATTTGTATAT TCATAAAATC GGGGTACCGA GCTCGAATTC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGGGGAT TCCTTAAGGT ATACTTTCCG CTGCATAACC CTGCTTCGGG GTCATTATAG CGATTTTTTC GGTATATCCA TCCTTTTTCG CACGATATAC AGGATTTTGC CAAAGGGTTC GTGTAGACTT TCCTTGGTGT ATCCAACGGC GTCAGCCGGG CAGGATAGGT GAAGTAGGCC CACCCGCGAG CGGGTGTTCC TTCTTCACTG TCCCTTATTC GCACCTGGCG GTGCTCAACG GGAATCCTGC TCTGCGAGGC TGGCCGATAA GCTAGCTTAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTC
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