pGL4.20-KLF6(human)-Promoter-Fluc2-SV40-Puro
$84.00
In stock
SKU
PL59122
100μg liquid plasmid(Instantly):$105
| name | pGL4.20-KLF6(human)-Promoter-Fluc2-SV40-Puro | another name | apGL4.20-KLF6(human)-Promoter-Fluc2-SV40-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Fluc |
| Promoter | KLF6 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | RVP3：CTAGCAAAATAGGCTGTCCC | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCGGGCCCCG GTCAATGGGC GCTTGGGCAA ATCTGTGGAT GGTTCAAAAC AAAGCCATTT GCATTCCTGG AAACAGAGTT AAGGTGCAGG TCCCCAAGAA GGGCTGGTCC TGGCTCTCAG TTGCTGGGGT AAATGTGATG AACACGAGCC AAGAACACTG TCAATTCACT CTGCATAACC TTCACCGCCC ACATACCGCA GGTACAAGAC AAACCTCCTT TCTCCACTCC AGACTCACTA AGGTCCCCAC CTAGTCTTAC AGCCACATGC TTCTGCCGGA CCCTCTAAAA CTTAACATAC AAAACCCTCT AATTTTTTTT TACATAGTCC ATTCTTTCAA AATGTTTAAT GTTCATTAAA CTATTTTGAA ACAAAAACTA TAATACAGTT ACAATTCAGT TTTCCATTTT GGATTTTAAA ATGCTCTGGT TCGAGTCTCG ACATTTTTCT TAAAAATGAA CATTACGGTA TATTTTAGGA GATAATTTAT ATGGAAAAAA ACACACACAC TAAATGTCGG CTTGCACCTA AATTGTTTAC ACGTTCATGG GAAAACCTAG CTGTTAACCT TGGTGAGGGC TAAGATCGCC CTCTACAAGA TAAACACGTT TCTCCCAATT CCAAGGGGAG CCTGCGTGCT CCCCTCCCCC ACTCTGGTGG CCCGGAGGAC AGAGGGGACC CTAGGACTGG AGGGGGACGG AGACGGGCTC AGGGATGGAG GGGGACAGAG AGGGACCCGG AGGTGGAGGA GGACAGAGAG GGGTACCCCA AGGTTAGAGA ACAGAGGATG ACTAGGGGGA TGTAGGGAAA CAGAGCGGCG CAGAAGAGGA CAGTTGGGGA CGGGGCGGCC CCGGGCATAG AAGGGGACAG TTGGCGACCG GGAAGCCCCC GGCGTAGAAG GGGACAGTTG GGGACGGGGC GGCCTCGGGC ATAGAAGGGG ACGGGGGGGA ACCGGGCGGC CCCGGGACCG GACGGCCGCC CGCAGGGTGA GCTGCCGGAA CCGGGGCTCG CGCGGCGACA GGGCCCGGCT TGGTGACCGG GACGGGCGTG GGGTGGCGGG ACCGGGCAGC GGGGAAGAGA GACCGGGACT GGGGACAGGC GACGCGGCCC GCCCCGGCTC TCCCAGCCCC GGCTCCCTTA GCTCCGGCCG CGGGCGCAGT GCAGGGCTCG GCCGCGCACC TCACAGCGCG GACTCCTCCG GCGCGCGGCC GCCCGCGCCT TATATACCCT GCTAAAAATA GCCTGCGAGG CCACTTTCCA ATCAGAACGA GCAGAGTTCG AATTGAGCCA ATGGTGCGGG CCGTGATGGA GAGTCTCGGT CACGCCAGGC GGGGCCGCTG TGTGTCGCCG CCTGCGATTG GCCGGAGCTG ACATCATCGG CGCGCAGCCC CCGGGCCGGG AGCCACGGGA TTGGGCCGCG CACGGGGCGG GCGCGGGGCG AGTCACGTGG GGGGAGGGGG AGTGGGGGGA GCCAGGCGGG GGCGGAGGGG GAGGCTGGCA GCGGAGCTTT GAATAGGGAA GTTTTGCAGG GGTTACGTTT GCAGTCAGTC CGGTGTTTGC AAATATTGTG TGGGCTCCGC GCGCTGCGGG CTGCGGGAGG GTCCGGCCGG GCGTCTCTGC GAGCCTGGAG TTTGCATGAA ACTTTCACCT GCGCTCCGGG GAGACTTTCG GCTCCGGCTC CCACCGCCTC GAGGATATCA AGATCTGGCC TCGGCGGCCA AGCTTGGCAA TCCGGTACTG TTGGTAAAGC CACCATGGAA GATGCCAAAA ACATTAAGAA GGGCCCAGCG CCATTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCAC CATCGCCTTT ACCGACGCAC ATATCGAGGT GGACATTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG CTATGAAGCG CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT TGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG GCCCCAGCTA ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG CCCACCGTCG TATTCGTGAG CAAGAAAGGG CTGCAAAAGA TCCTCAACGT GCAAAAGAAG CTACCGATCA TACAAAAGAT CATCATCATG GATAGCAAGA CCGACTACCA GGGCTTCCAA AGCATGTACA CCTTCGTGAC TTCCCATTTG CCACCCGGCT TCAACGAGTA CGACTTCGTG CCCGAGAGCT TCGACCGGGA CAAAACCATC GCCCTGATCA TGAACAGTAG TGGCAGTACC GGATTGCCCA AGGGCGTAGC CCTACCGCAC CGCACCGCTT GTGTCCGATT CAGTCATGCC CGCGACCCCA TCTTCGGCAA CCAGATCATC CCCGACACCG CTATCCTCAG CGTGGTGCCA TTTCACCACG GCTTCGGCAT GTTCACCACG CTGGGCTACT TGATCTGCGG CTTTCGGGTC GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA GCTTGCAAGA CTATAAGATT CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTTCTTCG CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT GCACGAGATC GCCAGCGGCG GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA ACGCTTCCAC CTACCAGGCA TCCGCCAGGG CTACGGCCTG ACAGAAACAA CCAGCGCCAT TCTGATCACC CCCGAAGGGG ACGACAAGCC TGGCGCAGTA GGCAAGGTGG TGCCCTTCTT CGAGGCTAAG GTGGTGGACT TGGACACCGG TAAGACACTG GGTGTGAACC AGCGCGGCGA GCTGTGCGTC CGTGGCCCCA TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA CGCTCTCATC GACAAGGACG GCTGGCTGCA CAGCGGCGAC ATCGCCTACT GGGACGAGGA CGAGCACTTC TTCATCGTGG ACCGGCTGAA GAGCCTGATC AAATACAAGG GCTACCAGGT AGCCCCAGCC GAACTGGAGA GCATCCTGCT GCAACACCCC AACATCTTCG ACGCCGGGGT CGCCGGCCTG CCCGACGACG ATGCCGGCGA GCTGCCCGCC GCAGTCGTCG TGCTGGAACA CGGTAAAACC ATGACCGAGA AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC CAAGAAGCTG CGCGGTGGTG TTGTGTTCGT GGACGAGGTG CCTAAAGGAC TGACCGGCAA GTTGGACGCC CGCAAGATCC GCGAGATTCT CATTAAGGCC AAGAAGGGCG GCAAGATCGC CGTGTAATAA TTCTAGAGTC GGGGCGGCCG GCCGCTTCGA GCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTAAAATCGA TAAGGATCCG TTTGCGTATT GGGCGCTCTT CCGCTGATCT GCGCAGCACC ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGCT ACCTTCTGAG GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCG ATTCTTCTGA CACTAGCGCC ACCATGACCG AGTACAAGCC TACCGTGCGC CTGGCCACTC GCGATGATGT GCCCCGCGCC GTCCGCACTC TGGCCGCCGC TTTCGCCGAC TACCCCGCTA CCCGGCACAC CGTGGACCCC GACCGGCACA TCGAGCGTGT GACAGAGTTG CAGGAGCTGT TCCTGACCCG CGTCGGGCTG GACATCGGCA AGGTGTGGGT AGCCGACGAC GGCGCGGCCG TGGCCGTGTG GACTACCCCC GAGAGCGTTG AGGCCGGCGC CGTGTTCGCC GAGATCGGCC CCCGAATGGC CGAGCTGAGC GGCAGCCGCC TGGCCGCCCA GCAGCAAATG GAGGGCCTGC TTGCCCCCCA TCGTCCCAAG GAGCCTGCCT GGTTTCTGGC CACTGTAGGA GTGAGCCCCG ACCACCAGGG CAAGGGCTTG GGCAGCGCCG TCGTGTTGCC CGGCGTAGAG GCCGCCGAAC GCGCCGGTGT GCCCGCCTTT CTCGAAACAA GCGCACCAAG AAACCTTCCA TTCTACGAGC GCCTGGGCTT CACCGTGACC GCCGATGTCG AGGTGCCCGA GGGACCTAGG ACCTGGTGTA TGACACGAAA ACCTGGCGCC TAATGATCTA GAACCGGTCA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGTTCG AACTAGATGC TGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GCGGCCGCAA ATGCTAAACC ACTGCAGTGG TTACCAGTGC TTGATCAGTG AGGCACCGAT CTCAGCGATC TGCCTATTTC GTTCGTCCAT AGTGGCCTGA CTCCCCGTCG TGTAGATCAC TACGATTCGT GAGGGCTTAC CATCAGGCCC CAGCGCAGCA ATGATGCCGC GAGAGCCGCG TTCACCGGCC CCCGATTTGT CAGCAATGAA CCAGCCAGCA GGGAGGGCCG AGCGAAGAAG TGGTCCTGCT ACTTTGTCCG CCTCCATCCA GTCTATGAGC TGCTGTCGTG ATGCTAGAGT AAGAAGTTCG CCAGTGAGTA GTTTCCGAAG AGTTGTGGCC ATTGCTACTG GCATCGTGGT ATCACGCTCG TCGTTCGGTA TGGCTTCGTT CAACTCTGGT TCCCAGCGGT CAAGCCGGGT CACATGATCA CCCATATTAT GAAGAAATGC AGTCAGCTCC TTAGGGCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCGG TGTTGTCGCT CATGGTAATG GCAGCACTAC ACAATTCTCT TACCGTCATG CCATCCGTAA GATGCTTTTC CGTGACCGGC GAGTACTCAA CCAAGTCGTT TTGTGAGTAG TGTATACGGC GACCAAGCTG CTCTTGCCCG GCGTCTATAC GGGACAACAC CGCGCCACAT AGCAGTACTT TGAAAGTGCT CATCATCGGG AATCGTTCTT CGGGGCGGAA AGACTCAAGG ATCTTGCCGC TATTGAGATC CAGTTCGATA TAGCCCACTC TTGCACCCAG TTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCGGGG TGTGCAAAAA CAGGCAAGCA AAATGCCGCA AAGAAGGGAA TGAGTGCGAC ACGAAAATGT TGGATGCTCA TACTCGTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTACTAGTAC GTCTCTCAAG GATAAGTAAG TAATATTAAG GTACGGGAGG TATTGGACAG GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGTACTAAC ATACGCTCTC CATCAAAACA AAACGAAACA AAACAAACTA GCAAAATAGG CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA CATTTCTCT
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