pEnCMV-CPD(human)-FLAG-SV40-Neo
$142.00
In stock
SKU
PL59081
NM_001304.5;GP180
100μg liquid plasmid(Instantly):$163
| name | pEnCMV-CPD(human)-FLAG-SV40-Neo | another name | apEnCMV-CPD(human)-FLAG-SV40-Neo |
| Insert Size (bp) | 4143bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC ATGGCGAGCG GCCGGGACGA GCGGCCGCCT TGGCGGCTAG GGCGGCTCCT GTTGCTCATG TGCCTGCTGC TGCTGGGGAG CTCGGCCCGG GCGGCTCACA TCAAGAAGGC GGAGGCGACT ACCACAACTA CGAGCGCGGG CGCCGAGGCG GCCGAGGGCC AGTTCGACCG CTACTACCAC GAAGAGGAGT TGGAGTCGGC GCTGAGGGAG GCGGCGGCCG CGGGCCTCCC CGGCCTGGCC CGCCTCTTTA GCATCGGCCG CTCGGTGGAA GGCCGGCCGC TGTGGGTGCT TCGCCTCACC GCCGGCCTGG GGTCGCTAAT CCCTGAGGGC GACGCGGGGC CTGACGCTGC CGGGCCCGAC GCTGCGGGGC CGCTGCTGCC CGGCCGGCCC CAGGTGAAGC TGGTGGGCAA CATGCATGGC GACGAGACCG TGTCGCGCCA GGTGTTGATC TACTTGGCCC GCGAGCTGGC GGCCGGCTAC CGCCGCGGGG ACCCGCGCCT GGTCCGCCTG CTCAACACCA CCGACGTGTA CCTGCTGCCC AGCCTCAACC CCGATGGCTT CGAGCGTGCC CGCGAGGGCG ACTGTGGCTT CGGCGACGGC GGCCCGTCCG GGGCCAGCGG CCGCGACAAT AGTCGCGGCC GCGACCTCAA CCGAAGCTTT CCCGACCAGT TTAGCACCGG CGAACCCCCC GCCCTGGACG AGGTGCCCGA GGTGCGCGCC CTCATCGAGT GGATCCGCAG GAACAAGTTT GTGCTTTCTG GAAATCTGCA TGGTGGCTCA GTGGTAGCAA GCTATCCTTT TGATGATTCT CCAGAACATA AGGCCACTGG AATCTATAGC AAAACCTCAG ATGATGAAGT ATTTAAATAC TTGGCAAAAG CTTATGCTTC AAACCACCCC ATAATGAAAA CTGGTGAGCC TCATTGTCCA GGAGATGAAG ACGAGACTTT CAAAGATGGA ATCACAAACG GCGCACATTG GTATGATGTG GAAGGTGGTA TGCAAGATTA CAATTATGTG TGGGCCAACT GTTTTGAGAT CACATTAGAA CTGTCTTGTT GCAAGTACCC ACCTGCTTCA CAGCTTCGAC AGGAATGGGA GAACAATCGT GAGTCTTTGA TCACATTGAT TGAAAAGGTT CACATTGGAG TGAAAGGATT TGTTAAAGAT TCCATAACAG GATCTGGGTT AGAGAATGCA ACCATCTCAG TGGCTGGTAT TAATCATAAT ATCACAACAG GCAGATTTGG TGATTTCTAC CGATTACTTG TTCCTGGAAC TTACAACCTT ACAGTAGTTT TAACTGGGTA TATGCCATTG ACTGTTACTA ATGTAGTGGT GAAAGAAGGA CCAGCCACAG AGGTGGATTT TTCTCTTAGG CCAACTGTAA CTTCAGTAAT CCCTGACACG ACAGAGGCTG TATCAACTGC TAGCACAGTT GCTATACCTA ATATTCTTTC TGGAACATCA TCCTCCTACC AGCCAATTCA GCCAAAGGAC TTTCACCACC ACCATTTCCC TGATATGGAA ATCTTCTTGA GAAGGTTTGC CAATGAATAT CCTAACATTA CCCGGCTTTA TTCCTTGGGA AAATCAGTAG AGTCAAGAGA ACTTTATGTG ATGGAGATAT CTGATAATCC GGGTGTCCAT GAACCAGGTG AACCAGAATT TAAGTACATT GGAAATATGC ATGGAAATGA AGTGGTTGGA AGAGAACTGC TGTTGAACCT CATAGAATAC CTTTGTAAGA ACTTTGGAAC AGACCCTGAA GTCACAGATT TGGTTCATAA CACTAGAATT CACCTTATGC CATCCATGAA TCCTGATGGG TATGAAAAGT CCCAGGAAGG AGATTCAATA AGTGTAATTG GCAGAAACAA CAGCAACAAC TTTGACCTGA ACCGAAATTT CCCAGACCAG TTTGTTCAGA TCACAGATCC TACGCAACCA GAAACTATTG CTGTAATGAG CTGGATGAAG TCCTATCCAT TTGTACTTTC AGCAAACCTG CATGGAGGTT CTTTGGTGGT TAACTACCCT TTTGATGATG ATGAACAAGG ACTTGCCACA TATAGTAAAT CACCAGATGA TGCTGTGTTC CAACAAATAG CACTTTCTTA TTCCAAGGAA AATTCCCAGA TGTTTCAAGG TAGACCTTGC AAGAATATGT ATCCTAATGA ATATTTTCCT CATGGAATAA CAAATGGAGC TAGTTGGTAT AATGTGCCAG GAGGAATGCA GGACTGGAAC TATTTACAAA CAAATTGCTT TGAAGTGACT ATTGAACTAG GTTGTGTGAA ATATCCACTT GAGAAAGAGC TGCCAAACTT TTGGGAACAG AATCGAAGAT CACTAATCCA GTTTATGAAA CAGGTTCATC AGGGCGTCAG AGGATTTGTT CTAGATGCCA CAGATGGCAG GGGTATATTA AATGCCACCA TTAGTGTTGC TGAGATTAAT CACCCAGTGA CTACTTACAA AACTGGAGAT TACTGGCGTC TCTTGGTTCC AGGAACTTAT AAAATCACAG CATCTGCTCG AGGGTATAAT CCAGTTACCA AGAATGTGAC TGTCAAGAGT GAAGGCGCTA TTCAGGTCAA CTTCACACTT GTTCGATCCT CAACAGATTC AAACAATGAA TCAAAGAAAG GAAAAGGGGC TAGCAGCAGC ACCAATGATG CCAGTGATCC AACTACTAAA GAGTTTGAAA CTTTAATTAA AGACCTTTCA GCGGAGAATG GTTTGGAAAG CCTCATGTTA CGCTCCTCCT CAAATCTGGC TCTGGCTCTT TATCGATACC ATTCCTACAA AGACTTATCA GAGTTTCTGA GAGGACTTGT AATGAACTAT CCACATATTA CAAATCTTAC CAATTTGGGA CAGAGCACTG AATATCGTCA CATTTGGTCC CTTGAAATCT CCAATAAGCC CAATGTATCT GAGCCTGAAG AACCAAAGAT TCGTTTTGTT GCTGGTATCC ATGGAAATGC GCCAGTTGGA ACTGAACTGC TTTTGGCTCT GGCAGAATTT CTCTGCCTGA ACTACAAAAA GAACCCAGCT GTTACCCAAT TGGTTGACAG GACTAGGATT GTGATTGTCC CTTCTCTAAA TCCAGATGGG CGAGAGAGAG CTCAAGAGAA AGACTGTACT TCAAAAATAG GACAAACAAA TGCTCGTGGC AAAGATTTGG ATACAGACTT CACAAATAAT GCCTCCCAAC CTGAGACCAA AGCCATCATT GAAAATTTGA TTCAAAAACA GGACTTTAGT CTTTCTGTTG CCTTAGATGG TGGTTCCATG CTGGTCACAT ATCCTTATGA CAAGCCAGTA CAGACAGTGG AAAATAAAGA GACTCTGAAG CATTTGGCAT CTCTTTATGC AAATAATCAT CCATCCATGC ACATGGGTCA GCCCAGTTGC CCAAATAAAT CAGATGAGAA TATTCCAGGA GGAGTAATGC GTGGAGCAGA ATGGCATAGT CACCTGGGCA GCATGAAGGA TTATAGTGTC ACCTATGGCC ATTGTCCGGA AATCACAGTA TACACAAGCT GCTGTTACTT TCCTAGTGCT GCACGACTCC CTTCCTTGTG GGCAGACAAT AAGAGATCTC TTCTTAGTAT GTTAGTGGAG GTTCACAAGG GAGTTCATGG ATTTGTTAAA GATAAGACTG GAAAGCCAAT CTCTAAAGCA GTCATTGTAC TTAATGAAGG AATAAAGGTA CAAACAAAAG AGGGAGGTTA TTTCCATGTA CTCTTAGCGC CAGGTGTCCA TAACATTATT GCCATCGCTG ATGGGTACCA GCAACAACAT TCACAGGTCT TTGTGCATCA TGATGCAGCT AGTTCTGTGG TGATAGTCTT TGACACAGAT AACCGGATAT TTGGTTTGCC AAGGGAGCTT GTGGTAACTG TATCAGGTGC TACTATGTCG GCATTGATCC TAACAGCTTG CATTATTTGG TGCATCTGCT CAATCAAGTC TAATAGACAC AAGGATGGCT TTCATCGGCT CAGGCAGCAT CATGATGAGT ATGAAGATGA AATTCGCATG ATGTCTACCG GCTCCAAGAA GTCCCTCCTA AGCCATGAGT TCCAGGATGA AACAGACACT GAAGAGGAAA CATTATATTC TAGCAAACAT CTCGAGGATT ACAAGGATGA CGACGATAAG TAGTGAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC
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