pMTL82151
$171.00
In stock
SKU
PL59039
100μg liquid plasmid(Instantly):$192
| name | pMTL82151 | another name | apMTL82151 |
| Insert Size (bp) | Plasmid host | FusiformBacillus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGAT AAAAAAATTG TAGATAAATT TTATAAAATA GTTTTATCTA CAATTTTTTT ATCAGGAAAC AGCTATGACC GCGGCCGCTG TATCCATATG ACCATGATTA CGAATTCGAG CTCGGTACCC GGGGATCCTC TAGAGTCGAC GTCACGCGTC CATGGAGATC TCGAGGCCTG CAGACATGCA AGCTTGGCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCTA GCATAAAAAT AAGAAGCCTG CATTTGCAGG CTTCTTATTT TTATGGCGCG CCGTTCTGAA TCCTTAGCTA ATGGTTCAAC AGGTAACTAT GACGAAGATA GCACCCTGGA TAAGTCTGTA ATGGATTCTA AGGCATTTAA TGAAGACGTG TATATAAAAT GTGCTAATGA AAAAGAAAAT GCGTTAAAAG AGCCTAAAAT GAGTTCAAAT GGTTTTGAAA TTGATTGGTA GTTTAATTTA ATATATTTTT TCTATTGGCT ATCTCGATAC CTATAGAATC TTCTGTTCAC TTTTGTTTTT GAAATATAAA AAGGGGCTTT TTAGCCCCTT TTTTTTAAAA CTCCGGAGGA GTTTCTTCAT TCTTGATACT ATACGTAACT ATTTTCGATT TGACTTCATT GTCAATTAAG CTAGTAAAAT CAATGGTTAA AAAACAAAAA ACTTGCATTT TTCTACCTAG TAATTTATAA TTTTAAGTGT CGAGTTTAAA AGTATAATTT ACCAGGAAAG GAGCAAGTTT TTTAATAAGG AAAAATTTTT CCTTTTAAAA TTCTATTTCG TTATATGACT AATTATAATC AAAAAAATGA AAATAAACAA GAGGTAAAAA CTGCTTTAGA GAAATGTACT GATAAAAAAA GAAAAAATCC TAGATTTACG TCATACATAG CACCTTTAAC TACTAAGAAA AATATTGAAA GGACTTCCAC TTGTGGAGAT TATTTGTTTA TGTTGAGTGA TGCAGACTTA GAACATTTTA AATTACATAA AGGTAATTTT TGCGGTAATA GATTTTGTCC AATGTGTAGT TGGCGACTTG CTTGTAAGGA TAGTTTAGAA ATATCTATTC TTATGGAGCA TTTAAGAAAA GAAGAAAATA AAGAGTTTAT ATTTTTAACT CTTACAACTC CAAATGTAAA AAGTTATGAT CTTAATTATT CTATTAAACA ATATAATAAA TCTTTTAAAA AATTAATGGA GCGTAAGGAA GTTAAGGATA TAACTAAAGG TTATATAAGA AAATTAGAAG TAACTTACCA AAAGGAAAAA TACATAACAA AGGATTTATG GAAAATAAAA AAAGATTATT ATCAAAAAAA AGGACTTGAA ATTGGTGATT TAGAACCTAA TTTTGATACT TATAATCCTC ATTTTCATGT AGTTATTGCA GTTAATAAAA GTTATTTTAC AGATAAAAAT TATTATATAA ATCGAGAAAG ATGGTTGGAA TTATGGAAGT TTGCTACTAA GGATGATTCT ATAACTCAAG TTGATGTTAG AAAAGCAAAA ATTAATGATT ATAAAGAGGT TTACGAACTT GCGAAATATT CAGCTAAAGA CACTGATTAT TTAATATCGA GGCCAGTATT TGAAATTTTT TATAAAGCAT TAAAAGGCAA GCAGGTATTA GTTTTTAGTG GATTTTTTAA AGATGCACAC AAATTGTACA AGCAAGGAAA ACTTGATGTT TATAAAAAGA AAGATGAAAT TAAATATGTC TATATAGTTT ATTATAATTG GTGCAAAAAA CAATATGAAA AAACTAGAAT AAGGGAACTT ACGGAAGATG AAAAAGAAGA ATTAAATCAA GATTTAATAG ATGAAATAGA AATAGATTAA AGTGTAACTA TACTTTATAT ATATATGATT AAAAAAATAA AAAACAACAG CCTATTAGGT TGTTGTTTTT TATTTTCTTT ATTAATTTTT TTAATTTTTA GTTTTTAGTT CTTTTTTAAA ATAAGTTTCA GCCTCTTTTT CAATATTTTT TAAAGAAGGA GTATTTGCAT GAATTGCCTT TTTTCTAACA GACTTAGGAA ATATTTTAAC AGTATCTTCT TGCGCCGGTG ATTTTGGAAC TTCATAACTT ACTAATTTAT AATTATTATT TTCTTTTTTA ATTGTAACAG TTGCAAAAGA AGCTGAACCT GTTCCTTCAA CTAGTTTATC ATCTTCAATA TAATATTCTT GACCTATATA GTATAAATAT ATTTTTATTA TATTTTTACT TTTTTCTGAA TCTATTATTT TATAATCATA AAAAGTTTTA CCACCAAAAG AAGGTTGTAC TCCTTCTGGT CCAACATATT TTTTTACTAT ATTATCTAAA TAATTTTTGG GAACTGGTGT TGTAATTTGA TTAATCGAAC AACCAGTTAT ACTTAAAGGA ATTATAACTA TAAAAATATA TAGGATTATC TTTTTAAATT TCATTATTGG CCTCCTTTTT ATTAAATTTA TGTTACCATA AAAAGGACAT AACGGGAATA TGTAGAATAT TTTTAATGTA GACAAAATTT TACATAAATA TAAAGAAAGG AAGTGTTTGT TTAAATTTTA TAGCAAACTA TCAAAAATTA GGGGGATAAA AATTTATGAA AAAAAGGTTT TCGATGTTAT TTTTATGTTT AACTTTAATA GTTTGTGGTT TATTTACAAA ATCGATTTCG GCCGGCCAGT GGGCAAGTTG AAAAATTCAC AAAAATGTGG TATAATATCT TTGTTCATTA GAGCGATAAA CTTGAATTTG AGAGGGAACT TAGATGGTAT TTGAAAAAAT TGATAAAAAT AGTTGGAACA GAAAAGAGTA TTTTGACCAC TACTTTGCAA GTGTACCTTG TACCTACAGC ATGACCGTTA AAGTGGATAT CACACAAATA AAGGAAAAGG GAATGAAACT ATATCCTGCA ATGCTTTATT ATATTGCAAT GATTGTAAAC CGCCATTCAG AGTTTAGGAC GGCAATCAAT CAAGATGGTG AATTGGGGAT ATATGATGAG ATGATACCAA GCTATACAAT ATTTCACAAT GATACTGAAA CATTTTCCAG CCTTTGGACT GAGTGTAAGT CTGACTTTAA ATCATTTTTA GCAGATTATG AAAGTGATAC GCAACGGTAT GGAAACAATC ATAGAATGGA AGGAAAGCCA AATGCTCCGG AAAACATTTT TAATGTATCT ATGATACCGT GGTCAACCTT CGATGGCTTT AATCTGAATT TGCAGAAAGG ATATGATTAT TTGATTCCTA TTTTTACTAT GGGGAAATAT TATAAAGAAG ATAACAAAAT TATACTTCCT TTGGCAATTC AAGTTCATCA CGCAGTATGT GACGGATTTC ACATTTGCCG TTTTGTAAAC GAATTGCAGG AATTGATAAA TAGTTAACTT CAGGTTTGTC TGTAACTAAA AACAAGTATT TAAGCAAAAA CATCGTAGAA ATACGGTGTT TTTTGTTACC CTAAGTTTAA ACTCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAGG GCCCCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GGCTACCGCC GGCGTAACAG ATGAGGGCAA GCGGATGGCT GATGAAACCA AGCCAACCAG GAAGGGCAGC CCACCTATCA AGGTGTACTG CCTTCCAGAC GAACGAAGAG CGATTGAGGA AAAGGCGGCG GCGGCCGGCA TGAGCCTGTC GGCCTACCTG CTGGCCGTCG GCCAGGGCTA CAAAATCACG GGCGTCGTGG ACTATGAGCA CGTCCGCGAG CTGGCCCGCA TCAATGGCGA CCTGGGCCGC CTGGGCGGCC TGCTGAAACT CTGGCTCACC GACGACCCGC GCACGGCGCG GTTCGGTGAT GCCACGATCC TCGCCCTGCT GGCGAAGATC GAAGAGAAGC AGGACGAGCT TGGCAAGGTC ATGATGGGCG TGGTCCGCCC GAGGGCAGAG CCATGACTTT TTTAGCCGCT AAAACGGCCG GGGGGTGCGC GTGATTGCCA AGCACGTCCC CATGCGCTCC ATCAAGAAGA GCGACTTCGC GGAGCTGGTG AAGTACATCA CCGACGAGCA AGGCAAGACC GATCGGGCCC
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