pCS2-6×Myc-FBXO4(human)(密码子优化)
$99.00
In stock
SKU
PL58672
NM_012176.3;FBX4
100μg liquid plasmid(Instantly):$120
| name | pCS2-6×Myc-FBXO4(human)(密码子优化) | another name | apCS2-6×Myc-FBXO4(human)(密码子优化) |
| Insert Size (bp) | 1164bp | Plasmid host | Mammary cell,Fish |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTTAAAGCTA TGGAGCAAAA GCTCATTTCT GAAGAGGACT TGAATGAAAT GGAGCAAAAG CTCATTTCTG AAGAGGACTT GAATGAAATG GAGCAAAAGC TCATTTCTGA AGAGGACTTG AATGAAATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAATGGA GCAAAAGCTC ATTTCTGAAG AGGACTTGAA TGAAATGGAG AGCTTGGGCG ACCTCACCAT GGAGCAAAAG CTCATTTCTG AAGAGGACTT GAATTCAATG GCTGGTTCTG AACCGCGTTC TGGTACCAAC TCTCCGCCGC CGCCGTTCTC TGACTGGGGT CGTCTGGAAG CTGCTATCCT GTCTGGTTGG AAAACCTTCT GGCAGTCTGT TTCTAAAGAA CGTGTTGCTC GTACCACCTC TCGTGAAGAA GTTGACGAAG CTGCTTCTAC CCTGACCCGT CTGCCGATCG ACGTTCAGCT GTACATCCTG TCTTTCCTGT CTCCGCACGA TCTGTGTCAG TTGGGAAGTA CAAATCATTA TTGGAATGAA ACTGTAAGAG ATCCAATTCT GTGGAGATAC TTTTTGTTGA GGGATCTTCC TTCTTGGTCT TCTGTTGACT GGAAGTCTCT TCCAGATCTA GAAATCTTAA AAAAGCCTAT ATCTGAGGTC ACTGATGGTG CATTTTTTGA CTACATGGCA GTCTATAGAA TGTGCTGTCC ATACACAAGA AGAGCTTCAA AATCCAGCCG TCCTATGTAT GGAGCTGTCA CTTCTTTTTT ACACTCCCTG ATCATTCAGA ATGAACCACG ATTTGCTATG TTTGGACCAG GTTTGGAAGA ATTGAATACC TCTTTGGTGT TGAGCTTGAT GTCTTCAGAG GAACTTTGCC CAACAGCTGG TTTGCCTCAG AGGCAGATTG ATGGTATTGG ATCAGGAGTC AATTTTCAGT TGAACAACCA ACATAAATTC AACATTCTAA TCTTATATTC AACTACCAGA AAGGAAAGAG ATAGAGCAAG GGAAGAGCAT ACAAGTGCAG TTAACAAGAT GTTCAGTCGA CACAATGAAG GTGATGATCA ACAAGGAAGC CGGTACAGTG TGATTCCACA GATTCAAAAA GTGTGTGAAG TTGTAGATGG GTTCATCTAT GTTGCAAATG CTGAAGCTCA TAAAAGACAT GAATGGCAAG ATGAATTTTC TCATATTATG GCAATGACAG ATCCAGCCTT TGGGTCTTCG GGAAGACCAT TGTTGGTTTT ATCTTGTATT TCTCAAGGGG ATGTAAAAAG AATGCCCTGT TTTTATTTGG CTCATGAGCT GCATCTGAAT CTTCTAAATC ACCCATGGCT GGTCCAGGAT ACAGAGGCTG AAACTCTGAC TGGTTTTTTG AATGGCATTG AGTGGATTCT TGAAGAAGTG GAATCTAAGC GTGCAAGATG ACTCGAGCCT CTAGAACTAT AGTGAGTCGT ATTACGTAGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAATTCG CGGCCGCGGC GCCAATGCAT TGGGCCCGGT ACCCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGTCGACCA TAGCCAATTC AATATGGCGT ATATGGACTC ATGCCAATTC AATATGGTGG ATCTGGACCT GTGCCAATTC AATATGGCGT ATATGGACTC GTGCCAATTC AATATGGTGG ATCTGGACCC CAGCCAATTC AATATGGCGG ACTTGGCACC ATGCCAATTC AATATGGCGG ACTTGGCACT GTGCCAACTG GGGAGGGGTC TACTTGGCAC GGTGCCAAGT TTGAGGAGGG GTCTTGGCCC TGTGCCAAGT CCGCCATATT GAATTGGCAT GGTGCCAATA ATGGCGGCCA TATTGGCTAT ATGCCAGGAT CAATATATAG GCAATATCCA ATATGGCCCT ATGCCAATAT GGCTATTGGC CAGGTTCAAT ACTATGTATT GGCCCTATGC CATATAGTAT TCCATATATG GGTTTTCCTA TTGACGTAGA TAGCCCCTCC CAATGGGCGG TCCCATATAC CATATATGGG GCTTCCTAAT ACCGCCCATA GCCACTCCCC CATTGACGTC AATGGTCTCT ATATATGGTC TTTCCTATTG ACGTCATATG GGCGGTCCTA TTGACGTATA TGGCGCCTCC CCCATTGACG TCAATTACGG TAAATGGCCC GCCTGGCTCA ATGCCCATTG ACGTCAATAG GACCACCCAC CATTGACGTC AATGGGATGG CTCATTGCCC ATTCATATCC GTTCTCACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCACTTGGCA GTACATCAAT ATCTATTAAT AGTAACTTGG CAAGTACATT ACTATTGGAA GGACGCCAGG GTACATTGGC AGTACTCCCA TTGACGTCAA TGGCGGTAAA TGGCCCGCGA TGGCTGCCAA GTACATCCCC ATTGACGTCA ATGGGGAGGG GCAATGACGC AAATGGGCGT TCCATTGACG TAAATGGGCG GTAGGCGTGC CTAATGGGAG GTCTATATAA GCAATGCTCG TTTAGGGAAC
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