pEGP2×35S-nYFP-NOS
$99.00
In stock
SKU
PL58510
100μg liquid plasmid(Instantly):$120
| name | pEGP2×35S-nYFP-NOS | another name | apEGP2×35S-nYFP-NOS |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Bimolecular fluorescence | Fragment type | BIFC |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | 2×35s | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGACTTTT CAACAAAGGG TAATATCGGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTC ATCAAAAGGA CAGTAGAAAA GGAAGGTGGC ACCTACAAAT GCCATCATTG CGATAAAGGA AAGGCTATCG TTCAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATAACATGG TGGAGCACGA CACTCTCGTC TACTCCAAGA ATATCAAAGA TACAGTCTCA GAAGACCAAA GGGCTATTGA GACTTTTCAA CAAAGGGTAA TATCGGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG TCACTTCATC AAAAGGACAG TAGAAAAGGA AGGTGGCACC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCTATCGTTC AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA AGCAAGTGGA TTGATGTGAT ATCTCCACTG ACGTAAGGGA TGACGCACAA TCCCACTATC CTTCGCAAGA CCTTCCTCTA TATAAGGAAG TTCATTTCAT TTGGAGAGGA CACGCTGAAA TCACCAGTCT CTCTCTACAA ATCTATCTCT CTCGAGCTTT CGCAGATCCC GGGGGGCAAT GAGATATGAA AAAGCCTGAA CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA GTTTAGCGAG AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA ACCGAACTGC CCGCTGTTCT ACAACCGGTC GCGGAGGCTA TGGATGCGAT CGCTGCGGCC GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT ACATGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCACGACT CCGGGCGTAT ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTGACGGCAA TTTCGATGAT GCAGCTTGGG CGCAGGGTCG ATGCGACGCA ATCGTCCGAT CCGGAGCCGG GACTGTCGGG CGTACACAAA TCGCCCGCAG AAGCGCGGCC GTCTGGACCG ATGGCTGTGT AGAAGTACTC GCCGATAGTG GAAACCGACG CCCCAGCACT CGTCCGAGGG CAAAGAAATA GAGTAGATGC CGACCGGATC TGTCGATCGA CAAGCTCGAG TTTCTCCATA ATAATGTGTG AGTAGTTCCC AGATAAGGGA ATTAGGGTTC CTATAGGGTT TCGCTCATGT GTTGAGCATA TAAGAAACCC TTAGTATGTA TTTGTATTTG TAAAATACTT CTATCAATAA AATTTCTAAT TCCTAAAACC AAAATCCAGT ACTAAAATCC AGATCCCCCG AATTAATTCG GCGTTAATTC AGTACATTAA AAACGTCCGC AATGTGTTAT TAAGTTGTCT AAGCGTCAAT TTGTTTACAC CACAATATAT CCTGCCACCA GCCAGCCAAC AGCTCCCCGA CCGGCAGCTC GGCACAAAAT CACCACTCGA TACAGGCAGC CCATCAGTCC GGGACGGCGT CAGCGGGAGA GCCGTTGTAA GGCGGCAGAC TTTGCTCATG TTACCGATGC TATTCGGAAG AACGGCAACT AAGCTGCCGG GTTTGAAACA CGGATGATCT CGCGGAGGGT AGCATGTTGA TTGTAACGAT GACAGAGCGT TGCTGCCTGT GATCACCGCG GTTTCAAAAT CGGCTCCGTC GATACTATGT TATACGCCAA CTTTGAAAAC AACTTTGAAA AAGCTGTTTT CTGGTATTTA AGGTTTTAGA ATGCAAGGAA CAGTGAATTG GAGTTCGTCT TGTTATAATT AGCTTCTTGG GGTATCTTTA AATACTGTAG AAAAGAGGAA GGAAATAATA AATGGCTAAA ATGAGAATAT CACCGGAATT GAAAAAACTG ATCGAAAAAT ACCGCTGCGT AAAAGATACG GAAGGAATGT CTCCTGCTAA GGTATATAAG CTGGTGGGAG AAAATGAAAA CCTATATTTA AAAATGACGG ACAGCCGGTA TAAAGGGACC ACCTATGATG TGGAACGGGA AAAGGACATG ATGCTATGGC TGGAAGGAAA GCTGCCTGTT CCAAAGGTCC TGCACTTTGA ACGGCATGAT GGCTGGAGCA ATCTGCTCAT GAGTGAGGCC GATGGCGTCC TTTGCTCGGA AGAGTATGAA GATGAACAAA GCCCTGAAAA GATTATCGAG CTGTATGCGG AGTGCATCAG GCTCTTTCAC TCCATCGACA TATCGGATTG TCCCTATACG AATAGCTTAG ACAGCCGCTT AGCCGAATTG GATTACTTAC TGAATAACGA TCTGGCCGAT GTGGATTGCG AAAACTGGGA AGAAGACACT CCATTTAAAG ATCCGCGCGA GCTGTATGAT TTTTTAAAGA CGGAAAAGCC CGAAGAGGAA CTTGTCTTTT CCCACGGCGA CCTGGGAGAC AGCAACATCT TTGTGAAAGA TGGCAAAGTA AGTGGCTTTA TTGATCTTGG GAGAAGCGGC AGGGCGGACA AGTGGTATGA CATTGCCTTC TGCGTCCGGT CGATCAGGGA GGATATCGGG GAAGAACAGT ATGTCGAGCT ATTTTTTGAC TTACTGGGGA TCAAGCCTGA TTGGGAGAAA ATAAAATATT ATATTTTACT GGATGAATTG TTTTAGTACC TAGAATGCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGGG TGCCTTGATG TGGGCGCCGG CGGTCGAGTG GCGACGGCGC GGCTTGTCCG CGCCCTGGTA GATTGCCTGG CCGTAGGCCA GCCATTTTTG AGCGGCCAGC GGCCGCGATA GGCCGACGCG AAGCGGCGGG GCGTAGGGAG CGCAGCGACC GAAGGGTAGG CGCTTTTTGC AGCTCTTCGG CTGTGCGCTG GCCAGACAGT TATGCACAGG CCAGGCGGGT TTTAAGAGTT TTAATAAGTT TTAAAGAGTT TTAGGCGGAA AAATCGCCTT TTTTCTCTTT TATATCAGTC ACTTACATGT GTGACCGGTT CCCAATGTAC GGCTTTGGGT TCCCAATGTA CGGGTTCCGG TTCCCAATGT ACGGCTTTGG GTTCCCAATG TACGTGCTAT CCACAGGAAA CCTTTTCGAC CTTTTTCCCC TGCTAGGGCA ATTTGCCCTA GCATCTGCTC CGTACATTAG GAACCGGCGG ATGCTTCGCC CTCGATCAGG TTGCGGTAGC GCATGACTAG GATCGGGCCA GCCTGCCCCG CCTCCTCCTT CAAATCGTAC TCCGGCAGGT CATTTGACCC GATCAGCTTG CGCACGGTGA AACAGAACTT CTTGAACTCT CCGGCGCTGC CACTGCGTTC GTAGATCGTC TTGAACAACC ATCTGGCTTC TGCCTTGCCT GCGGCGCGGC GTGCCAGGCG GTAGAGAAAA CGGCCGATGC CGGGATCGAT CAAAAAGTAA TCGGGGTGAA CCGTCAGCAC GTCCGGGTTC TTGCCTTCTG TGATCTCGCG GTACATCCAA TCAGCTAGCT CGATCTCGAT GTACTCCGGC CGCCCGGTTT CGCTCTTTAC GATCTTGTAG CGGCTAATCA AGGCTTCACC CTCGGATACC GTCACCAGGC GGCCGTTCTT GGCCTTCTTC GTACGCTGCA TGGCAACGTG CGTGGTGTTT AACCGAATGC AGGTTTCTAC CAGGTCGTCT TTCTGCTTTC CGCCATCGGC TCGCCGGCAG AACTTGAGTA CGTCCGCAAC GTGTGGACGG AACACGCGGC CGGGCTTGTC TCCCTTCCCT TCCCGGTATC GGTTCATGGA TTCGGTTAGA TGGGAAACCG CCATCAGTAC CAGGTCGTAA TCCCACACAC TGGCCATGCC GGCCGGCCCT GCGGAAACCT CTACGTGCCC GTCTGGAAGC TCGTAGCGGA TCACCTCGCC AGCTCGTCGG TCACGCTTCG ACAGACGGAA AACGGCCACG TCCATGATGC TGCGACTATC GCGGGTGCCC ACGTCATAGA GCATCGGAAC GAAAAAATCT GGTTGCTCGT CGCCCTTGGG CGGCTTCCTA ATCGACGGCG CACCGGCTGC CGGCGGTTGC CGGGATTCTT TGCGGATTCG ATCAGCGGCC GCTTGCCACG ATTCACCGGG GCGTGCTTCT GCCTCGATGC GTTGCCGCTG GGCGGCCTGC GCGGCCTTCA ACTTCTCCAC CAGGTCATCA CCCAGCGCCG CGCCGATTTG TACCGGGCCG GATGGTTTGC GACCGTCACG CCGATTCCTC GGGCTTGGGG GTTCCAGTGC CATTGCAGGG CCGGCAGACA ACCCAGCCGC TTACGCCTGG CCAACCGCCC GTTCCTCCAC ACATGGGGCA TTCCACGGCG TCGGTGCCTG GTTGTTCTTG ATTTTCCATG CCGCCTCCTT TAGCCGCTAA AATTCATCTA CTCATTTATT CATTTGCTCA TTTACTCTGG TAGCTGCGCG ATGTATTCAG ATAGCAGCTC GGTAATGGTC TTGCCTTGGC GTACCGCGTA CATCTTCAGC TTGGTGTGAT CCTCCGCCGG CAACTGAAAG TTGACCCGCT TCATGGCTGG CGTGTCTGCC AGGCTGGCCA ACGTTGCAGC CTTGCTGCTG CGTGCGCTCG GACGGCCGGC ACTTAGCGTG TTTGTGCTTT TGCTCATTTT CTCTTTACCT CATTAACTCA AATGAGTTTT GATTTAATTT CAGCGGCCAG CGCCTGGACC TCGCGGGCAG CGTCGCCCTC GGGTTCTGAT TCAAGAACGG TTGTGCCGGC GGCGGCAGTG CCTGGGTAGC TCACGCGCTG CGTGATACGG GACTCAAGAA TGGGCAGCTC GTACCCGGCC AGCGCCTCGG CAACCTCACC GCCGATGCGC GTGCCTTTGA TCGCCCGCGA CACGACAAAG GCCGCTTGTA GCCTTCCATC CGTGACCTCA ATGCGCTGCT TAACCAGCTC CACCAGGTCG GCGGTGGCCC ATATGTCGTA AGGGCTTGGC TGCACCGGAA TCAGCACGAA GTCGGCTGCC TTGATCGCGG ACACAGCCAA GTCCGCCGCC TGGGGCGCTC CGTCGATCAC TACGAAGTCG CGCCGGCCGA TGGCCTTCAC GTCGCGGTCA ATCGTCGGGC GGTCGATGCC GACAACGGTT AGCGGTTGAT CTTCCCGCAC GGCCGCCCAA TCGCGGGCAC TGCCCTGGGG ATCGGAATCG ACTAACAGAA CATCGGCCCC GGCGAGTTGC AGGGCGCGGG CTAGATGGGT TGCGATGGTC GTCTTGCCTG ACCCGCCTTT CTGGTTAAGT ACAGCGATAA CCTTCATGCG TTCCCCTTGC GTATTTGTTT ATTTACTCAT CGCATCATAT ACGCAGCGAC CGCATGACGC AAGCTGTTTT ACTCAAATAC ACATCACCTT TTTAGACGGC GGCGCTCGGT TTCTTCAGCG GCCAAGCTGG CCGGCCAGGC CGCCAGCTTG GCATCAGACA AACCGGCCAG GATTTCATGC AGCCGCACGG TTGAGACGTG CGCGGGCGGC TCGAACACGT ACCCGGCCGC GATCATCTCC GCCTCGATCT CTTCGGTAAT GAAAAACGGT TCGTCCTGGC CGTCCTGGTG CGGTTTCATG CTTGTTCCTC TTGGCGTTCA TTCTCGGCGG CCGCCAGGGC GTCGGCCTCG GTCAATGCGT CCTCACGGAA GGCACCGCGC CGCCTGGCCT CGGTGGGCGT CACTTCCTCG CTGCGCTCAA GTGCGCGGTA CAGGGTCGAG CGATGCACGC CAAGCAGTGC AGCCGCCTCT TTCACGGTGC GGCCTTCCTG GTCGATCAGC TCGCGGGCGT GCGCGATCTG TGCCGGGGTG AGGGTAGGGC GGGGGCCAAA CTTCACGCCT CGGGCCTTGG CGGCCTCGCG CCCGCTCCGG GTGCGGTCGA TGATTAGGGA ACGCTCGAAC TCGGCAATGC CGGCGAACAC GGTCAACACC ATGCGGCCGG CCGGCGTGGT GGTGTCGGCC CACGGCTCTG CCAGGCTACG CAGGCCCGCG CCGGCCTCCT GGATGCGCTC GGCAATGTCC AGTAGGTCGC GGGTGCTGCG GGCCAGGCGG TCTAGCCTGG TCACTGTCAC AACGTCGCCA GGGCGTAGGT GGTCAAGCAT CCTGGCCAGC TCCGGGCGGT CGCGCCTGGT GCCGGTGATC TTCTCGGAAA ACAGCTTGGT GCAGCCGGCC GCGTGCAGTT CGGCCCGTTG GTTGGTCAAG TCCTGGTCGT CGGTGCTGAC GCGGGCATAG CCCAGCAGGC CAGCGGCGGC GCTCTTGTTC ATGGCGTAAT GTCTCCGGTT CTAGTCGCAA GTATTCTACT TTATGCGACT AAAACACGCG ACAAGAAAAC GCCAGGAAAA GGGCAGGGCG GCAGCCTGTC GCGTAACTTA GGACTTGTGC GACATGTCGT TTTCAGAAGA CGGCTGCACT GAACGTCAGA AGCCGACTGC ACTATAGCAG CGGAGGGGTT GGATCAAAGT ACTTTGATCC CGAGGGGAAC CCTGTGGTTG GCATGCACAT ACAAATGGAC GAACGGATAA ACCTTTTCAC GCCCTTTTAA ATATCCGTTA TTCTAATAAA CGCTCTTTTC TCTTAGGTTT ACCCGCCAAT ATATCCTGTC AAACACTGAT AGTTTAAACT GAAGGCGGGA AACGACAATC TGATCCAAGC TCAAGCTGCT CTAGCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GCCAAGCTTA CCGATGCCTG CAGGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATAACATGG TGGAGCACGA CACACTTGTC TACTCCAAAA ATATCAAAGA TACAGTCTCA GAAGACCAAA GGGCAATTGA GACTTTTCAA CAAAGGGTAA TATCCGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG TCACTTTATT GTGAAGATAG TGGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCCATCGTTG AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA AGCAAGTGGA TTGATGTGAT ATCTCCACTG ACGTAAGGGA TGACGCACAA TCCCACTATC CTTCGCAAGA CCCTTCCTCT ATATAAGGAA GTTCATTTCA TTTGGAGAGG ACCTCGACCT CAACACAACA TATACAAAAC AAACGAATCT CAAGCAATCA AGCATTCTAC TTCTATTGCA GCAATTTAAA TCATTTCTTT TAAAGCAAAA GCAATTTTCT GAAAATTTTC ACCATTTACG AACGATAGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCTTCGG CTACGGCCTG CAGTGCTTCG CCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGTAACGGTG ATCCTCCCGA TCGTTCAAAC ATTTGGCAAT AAAGTTTCTT AAGATTGAAT CCTGTTGCCG GTCTTGCGAT GATTATCATA TAATTTCTGT TGAATTACGT TAAGCATGTA ATAATTAACA TGTAATGCAT GACGTTATTT ATGAGGTGGG TTTTTATGAT TAGAGTCCCG CAATTATACA TTTAATACGC GATAGAAAAC AAAATATAGC GCGCAAACTA GGATAAATTA TCGCGCGCGG TGTCATCTAT GTTACTAGAT CGGGAGCACC GGTAAGGCGC GCCGTAGTGA GAATTCGTAA TCATGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG CTAGAGCAGC TTGCCAACAT GGTGGAGCAC GACACTCTCG TCTACTCCAA GAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCTAT
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