pGBKT7-MP58
$84.00
In stock
SKU
PL58439
100μg liquid plasmid(Instantly):$105
| name | pGBKT7-MP58 | another name | apGBKT7-MP58 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | TRP1 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGTGCGGGC CTCTTCGCTA TTACGCCAGA TCCTTTTGTT GTTTCCGGGT GTACAATATG GACTTCCTCT TTTCTGGCAA CCAAACCCAT ACATCGGGAT TCCTATAATA CCTTCGTTGG TCTCCCTAAC ATGTAGGTGG CGGAGGGGAG ATATACAATA GAACAGATAC CAGACAAGAC ATAATGGGCT AAACAAGACT ACACCAATTA CACTGCCTCA TTGATGGTGG TACATAACGA ACTAATACTG TAGCCCTAGA CTTGATAGCC ATCATCATAT CGAAGTTTCA CTACCCTTTT TCCATTTGCC ATCTATTGAA GTAATAATAG GCGCATGCAA CTTCTTTTCT TTTTTTTTCT TTTCTCTCTC CCCCGTTGTT GTCTCACCAT ATCCGCAATG ACAAAAAAAT GATGGAAGAC ACTAAAGGAA AAAATTAACG ACAAAGACAG CACCAACAGA TGTCGTTGTT CCAGAGCTGA TGAGGGGTAT CTCGAAGCAC ACGAAACTTT TTCCTTCCTT CATTCACGCA CACTACTCTC TAATGAGCAA CGGTATACGG CCTTCCTTCC AGTTACTTGA ATTTGAAATA AAAAAAAGTT TGCTGTCTTG CTATCAAGTA TAAATAGACC TGCAATTATT AATCTTTTGT TTCCTCGTCA TTGTTCTCGT TCCCTTTCTT CCTTGTTTCT TTTTCTGCAC AATATTTCAA GCTATACCAA GCATACAATC AACTCCAAGC TTGAAGCAAG CCTCCTGAAA GATGAAGCTA CTGTCTTCTA TCGAACAAGC ATGCGATATT TGCCGACTTA AAAAGCTCAA GTGCTCCAAA GAAAAACCGA AGTGCGCCAA GTGTCTGAAG AACAACTGGG AGTGTCGCTA CTCTCCCAAA ACCAAAAGGT CTCCGCTGAC TAGGGCACAT CTGACAGAAG TGGAATCAAG GCTAGAAAGA CTGGAACAGC TATTTCTACT GATTTTTCCT CGAGAAGACC TTGACATGAT TTTGAAAATG GATTCTTTAC AGGATATAAA AGCATTGTTA ACAGGATTAT TTGTACAAGA TAATGTGAAT AAAGATGCCG TCACAGATAG ATTGGCTTCA GTGGAGACTG ATATGCCTCT AACATTGAGA CAGCATAGAA TAAGTGCGAC ATCATCATCG GAAGAGAGTA GTAACAAAGG TCAAAGACAG TTGACTGTAT CGCCGGAATT TGTAATACGA CTCACTATAG GGCGAGCCGC CATCATGGAG GAGCAGAAGC TGATCTCAGA GGAGGACCTG CATATGATGG CTTTCAAGGA AATCGCAATG TTTTCCAGTT TAGTGGTGGT CATCATAAGC TACAATGTAC TAGAAAGCAA TGCATTAATG ATGCAACCAT TAATAGACCA AGATTTCTGT GCAGTAAAAG CTAGTTTGTA CGAATTGGGA GAGGCTGAGA TGAACTTGAT GGACGATTCG CAGACATTAA ACGACATGCA AAGAGAATAT TTTGCTGGAA AAGTTGACTA TTCTGCTGTG GAAAGAGCAA GGAATGAACT TAATAAGTCC AAAAACAAGT GGTTCCAAAA AGTTATACAA TATCTCAAGG CAACAAATGA ATTTGAGCCC ACAATCAACT ATCAAACCTC AGATCCACAA AAAGTTTACC ATCAAATGGA TGACTTGGAC AATTATAAAG ATGCTCTAGA TGATATGCAT GAAAATATCC TTAAATCAAT GTCCCCTACC GTAGCCCAAC CTGTTGTAGC ATAAGGATCC GTCGACCTGC AGCGGCCGCA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCGCGCTTGC AGCCAAGCTA ATTCCGGGCG AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT AAGTGTATAC AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TATTCTTGAG TAACTCTTTC CTGTAGGTCA GGTTGCTTTC TCAGGTATAG CATGAGGTCG CTCTTATTGA CCACACCTCT ACCGGCATGC AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGGTCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG AGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAACGAAGC ATCTGTGCTT CATTTTGTAG AACAAAAATG CAACGCGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA CAGAACAGAA ATGCAACGCG AAAGCGCTAT TTTACCAACG AAGAATCTGT GCTTCATTTT TGTAAAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA ACAAAGAATC TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAGAGC GCTATTTTAC CAACAAAGAA TCTATACTTC TTTTTTGTTC TACAAAAATG CATCCCGAGA GCGCTATTTT TCTAACAAAG CATCTTAGAT TACTTTTTTT CTCCTTTGTG CGCTCTATAA TGCAGTCTCT TGATAACTTT TTGCACTGTA GGTCCGTTAA GGTTAGAAGA AGGCTACTTT GGTGTCTATT TTCTCTTCCA TAAAAAAAGC CTGACTCCAC TTCCCGCGTT TACTGATTAC TAGCGAAGCT GCGGGTGCAT TTTTTCAAGA TAAAGGCATC CCCGATTATA TTCTATACCG ATGTGGATTG CGCATACTTT GTGAACAGAA AGTGATAGCG TTGATGATTC TTCATTGGTC AGAAAATTAT GAACGGTTTC TTCTATTTTG TCTCTATATA CTACGTATAG GAAATGTTTA CATTTTCGTA TTGTTTTCGA TTCACTCTAT GAATAGTTCT TACTACAATT TTTTTGTCTA AAGAGTAATA CTAGAGATAA ACATAAAAAA TGTAGAGGTC GAGTTTAGAT GCAAGTTCAA GGAGCGAAAG GTGGATGGGT AGGTTATATA GGGATATAGC ACAGAGATAT ATAGCAAAGA GATACTTTTG AGCAATGTTT GTGGAAGCGG TATTCGCAAT ATTTTAGTAG CTCGTTACAG TCCGGTGCGT TTTTGGTTTT TTGAAAGTGC GTCTTCAGAG CGCTTTTGGT TTTCAAAAGC GCTCTGAAGT TCCTATACTT TCTAGAGAAT AGGAACTTCG GAATAGGAAC TTCAAAGCGT TTCCGAAAAC GAGCGCTTCC GAAAATGCAA CGCGAGCTGC GCACATACAG CTCACTGTTC ACGTCGCACC TATATCTGCG TGTTGCCTGT ATATATATAT ACATGAGAAG AACGGCATAG TGCGTGTTTA TGCTTAAATG CGTACTTATA TGCGTCTATT TATGTAGGAT GAAAGGTAGT CTAGTACCTC CTGTGATATT ATCCCATTCC ATGCGGGGTA TCGTATGCTT CCTTCAGCAC TACCCTTTAG CTGTTCTATA TGCTGCCACT CCTCAATTGG ATTAGTCTCA TCCTTCAATG CTATCATTTC CTTTGATATT GGATCATACT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAG ATCAACGACA TTACTATATA TATAATATAG GAAGCATTTA ATAGAACAGC ATCGTAATAT ATGTGTACTT TGCAGTTATG ACGCCAGATG GCAGTAGTGG AAGATATTCT TTATTGAAAA ATAGCTTGTC ACCTTACGTA CAATCTTGAT CCGGAGCTTT TCTTTTTTTG CCGATTAAGA ATTAATTCGG TCGAAAAAAG AAAAGGAGAG GGCCAAGAGG GAGGGCATTG GTGACTATTG AGCACGTGAG TATACGTGAT TAAGCACACA AAGGCAGCTT GGAGTATGTC TGTTATTAAT TTCACAGGTA GTTCTGGTCC ATTGGTGAAA GTTTGCGGCT TGCAGAGCAC AGAGGCCGCA GAATGTGCTC TAGATTCCGA TGCTGACTTG CTGGGTATTA TATGTGTGCC CAATAGAAAG AGAACAATTG ACCCGGTTAT TGCAAGGAAA ATTTCAAGTC TTGTAAAAGC ATATAAAAAT AGTTCAGGCA CTCCGAAATA CTTGGTTGGC GTGTTTCGTA ATCAACCTAA GGAGGATGTT TTGGCTCTGG TCAATGATTA CGGCATTGAT ATCGTCCAAC TGCATGGAGA TGAGTCGTGG CAAGAATACC AAGAGTTCCT CGGTTTGCCA GTTATTAAAA GACTCGTATT TCCAAAAGAC TGCAACATAC TACTCAGTGC AGCTTCACAG AAACCTCATT CGTTTATTCC CTTGTTTGAT TCAGAAGCAG GTGGGACAGG TGAACTTTTG GATTGGAACT CGATTTCTGA CTGGGTTGGA AGGCAAGAGA GCCCCGAAAG CTTACATTTT ATGTTAGCTG GTGGACTGAC GCCAGAAAAT GTTGGTGATG CGCTTAGATT AAATGGCGTT ATTGGTGTTG ATGTAAGCGG AGGTGTGGAG ACAAATGGTG TAAAAGACTC TAACAAAATA GCAAATTTCG TCAAAAATGC TAAGAAATAG GTTATTACTG AGTAGTATTT ATTTAAGTAT TGTTTGTGCA CTTGCCGATC TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGAT
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