pEnCMV-GPR158(human)-SV40-Neo
$99.00
In stock
SKU
PL58386
NM_020752.3
100μg liquid plasmid(Instantly):$120
| name | pEnCMV-GPR158(human)-SV40-Neo | another name | apEnCMV-GPR158(human)-SV40-Neo |
| Insert Size (bp) | 3648bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CGCCACCATG GGAGCCATGG CTTACCCCTT ACTCCTCTGC CTCCTGCTTG CTCAGCTGGG ATTGGGAGCT GTTGGCGCCA GCCGCGACCC CCAAGGACGG CCGGATTCCC CTCGAGAGAG GACCCCGAAG GGGAAGCCGC ACGCCCAGCA GCCGGGTCGA GCCTCTGCCT CGGACTCCTC GGCTCCCTGG AGCCGCTCCA CCGATGGCAC CATCTTGGCG CAGAAACTCG CCGAGGAGGT GCCCATGGAC GTGGCCTCTT ACCTCTACAC CGGGGACTCC CACCAGCTGA AGCGAGCCAA CTGCTCCGGC CGCTACGAGT TGGCGGGCCT GCCGGGGAAG TGGCCAGCCC TGGCCAGCGC GCACCCCTCC TTGCACCGGG CGCTGGACAC ACTGACACAC GCCACCAACT TCCTCAACGT GATGCTGCAG AGCAATAAGT CGCGGGAGCA GAACTTGCAG GACGACCTGG ATTGGTACCA GGCGCTGGTG TGGAGCCTTC TGGAGGGCGA GCCCAGCATC TCCCGGGCGG CCATCACCTT CAGCACCGAT TCGCTGTCCG CACCGGCCCC ACAGGTCTTC CTCCAGGCCA CGCGCGAGGA GAGCCGCATC CTGCTCCAAG ACCTGTCCTC CTCCGCACCC CACCTGGCCA ACGCCACTCT GGAGACCGAG TGGTTCCACG GCCTCCGGCG CAAGTGGAGG CCCCACTTAC ACCGCCGCGG CCCCAATCAG GGGCCCCGGG GCCTGGGCCA CAGCTGGCGG CGCAAGGACG GGCTCGGCGG GGACAAGAGC CACTTCAAGT GGTCTCCGCC TTATCTGGAG TGCGAGAACG GGAGTTACAA GCCCGGGTGG CTGGTTACTC TTTCCTCTGC CATCTACGGG TTGCAGCCTA ACCTGGTCCC GGAATTCAGG GGTGTCATGA AAGTTGACAT AAATCTTCAG AAAGTGGACA TTGACCAATG CTCAAGTGAT GGCTGGTTTT CAGGAACTCA TAAATGCCAC CTCAACAATT CAGAGTGTAT GCCAATTAAA GGCCTAGGAT TCGTTCTTGG AGCCTATGAG TGCATTTGCA AAGCAGGATT CTATCATCCT GGAGTCTTAC CAGTGAACAA CTTTCGGAGA AGGGGTCCGG ATCAGCATAT TTCAGGAAGT ACAAAAGATG TGTCAGAAGA AGCCTATGTC TGCCTACCTT GCAGGGAGGG CTGCCCCTTC TGTGCTGATG ACAGCCCATG CTTCGTCCAG GAAGATAAGT ATTTACGACT TGCCATCATC TCCTTCCAAG CCCTGTGTAT GCTGCTCGAC TTCGTTAGCA TGCTGGTGGT CTACCACTTT CGCAAAGCAA AGAGCATCCG GGCATCGGGC CTTATCCTGT TGGAAACGAT CCTTTTTGGA TCTCTGCTCC TATACTTTCC AGTTGTTATT TTGTACTTTG AGCCAAGCAC ATTTCGCTGT ATTCTCCTAA GATGGGCTCG TCTTCTCGGT TTTGCTACTG TTTACGGAAC TGTCACTCTC AAACTTCACA GGGTTTTGAA GGTGTTTCTT TCACGAACGG CTCAACGAAT TCCATATATG ACTGGCGGAC GGGTCATGAG GATGCTGGCA GTAATACTCT TGGTAGTGTT TTGGTTTCTC ATTGGCTGGA CTTCATCTGT GTGCCAGAAT TTGGAGAAAC AGATTTCACT TATTGGCCAG GGGAAAACAT CCGATCACCT CATCTTCAAT ATGTGCCTCA TTGACCGCTG GGACTACATG ACAGCAGTTG CTGAATTTTT ATTCCTCTTG TGGGGTGTTT ATCTCTGCTA TGCAGTGCGG ACAGTCCCAT CGGCATTCCA TGAGCCCCGC TATATGGCTG TTGCAGTTCA CAATGAGCTC ATCATCTCTG CTATATTCCA TACAATTAGA TTTGTTCTTG CCTCAAGACT TCAGTCTGAT TGGATGTTGA TGCTGTATTT TGCACATACT CATTTGACTG TGACAGTCAC CATTGGGTTG CTTTTGATTC CAAAGTTTTC ACATTCAAGC AATAACCCAC GAGATGATAT TGCTACAGAA GCATATGAGG ATGAGCTAGA CATGGGCCGA TCTGGATCCT ACCTGAACAG CAGTATCAAT TCAGCCTGGA GTGAGCACAG CTTGGATCCA GAGGACATTC GGGACGAGCT GAAAAAACTC TATGCCCAAC TGGAAATATA TAAAAGAAAG AAGATGATCA CAAACAACCC CCACCTCCAG AAAAAGCGGT GCTCGAAGAA GGGCCTAGGT CGTTCCATCA TGAGACGCAT TACGGAGATC CCAGAGACAG TCAGCCGGCA GTGCTCTAAA GAGGACAAGG AGGGCGCCGA CCATGGCACA GCCAAAGGCA CTGCCCTCAT CAGGAAGAAC CCCCCAGAGT CTTCAGGGAA CACAGGGAAA TCCAAGGAGG AGACCCTGAA AAACCGAGTC TTCTCACTCA AGAAATCCCA CAGCACTTAT GACCACGTGA GAGACCAAAC GGAAGAGTCC AGTAGCCTAC CCACAGAAAG CCAAGAGGAG GAGACAACAG AAAATTCCAC ACTGGAATCC CTGTCGGGTA AAAAACTAAC ACAAAAACTA AAAGAAGACA GCGAGGCTGA GTCCACGGAG TCGGTGCCGT TGGTGTGCAA GTCAGCAAGC GCTCACAACC TCAGCTCAGA GAAGAAAACT GGGCACCCAC GAACATCGAT GTTACAGAAG TCTCTCAGTG TCATAGCAAG CGCCAAGGAG AAGACTCTTG GATTAGCTGG GAAAACCCAA ACAGCAGGTG TGGAAGAACG CACTAAATCC CAGAAACCTT TGCCAAAAGA TAAAGAGACA AACAGAAATC ACTCAAATTC TGATAACACA GAGACTAAAG ATCCTGCCCC CCAAAACTCA AATCCTGCGG AGGAGCCAAG AAAGCCTCAG AAATCTGGGA TTATGAAACA ACAAAGGGTC AACCCCACCA CTGCCAATTC TGACCTGAAC CCAGGCACCA CCCAGATGAA GGACAACTTT GACATTGGGG AGGTGTGTCC TTGGGAGGTT TATGACCTGA CCCCTGGTCC TGTGCCTTCA GAATCAAAAG TTCAAAAGCA CGTATCTATT GTGGCTTCTG AAATGGAGAA AAACCCCACT TTTTCCTTAA AGGAGAAATC TCACCACAAG CCTAAGGCAG CTGAGGTTTG TCAGCAATCC AATCAGAAGC GCATAGATAA GGCTGAAGTA TGCCTTTGGG AGAGCCAAGG CCAGTCCATT TTGGAAGATG AGAAGCTTTT GATTTCCAAG ACTCCAGTTC TCCCAGAGAG GGCAAAAGAG GAGAACGGAG GTCAGCCTCG TGCAGCCAAT GTGTGTGCTG GGCAGAGCGA AGAACTGCCC CCCAAAGCTG TAGCATCAAA AACAGAGAAT GAAAATCTCA ACCAAATAGG ACACCAGGAA AAAAAGACAT CTTCTTCTGA GGAGAATGTG CGTGGCTCCT ATAACTCAAG TAATAACTTC CAGCAACCTT TAACATCACG AGCAGAGGTT TGTCCTTGGG AGTTTGAGAC CCCAGCTCAA CCAAATGCTG GAAGAAGTGT AGCTTTACCT GCCTCTTCTG CTCTAAGTGC AAATAAGATA GCAGGGCCTA GGAAAGAAGA GATCTGGGAT AGTTTTAAAG TGTAGCTCGA GTCTAGAGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG GT
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