pCMV-ACOT11(human)
$70.00
In stock
SKU
PL58337
NM_015547.4;BFIT; STARD14; THEA; THEM1
100μg liquid plasmid(Instantly):$91
| name | pCMV-ACOT11(human) | another name | apCMV-ACOT11(human) |
| Insert Size (bp) | 1824bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GACTCTACTA GAGGATCGCT AGCGCTACCG GACTCAGATC TCGAGCCACC ATGATCCAGA ATGTCGGAAA TCACCTGCGA CGGGGCTTGG CCTCTGTGTT CTCCAACCGC ACATCCCGGA AGTCAGCCTT ACGTGCGGGG AACGACAGTG CCATGGCAGA CGGCGAGGGA TACCGGAACC CCACGGAGGT GCAGATGAGC CAGCTGGTGC TGCCCTGCCA CACCAACCAA CGTGGTGAGC TGAGCGTCGG GCAGCTGCTC AAGTGGATTG ACACCACGGC TTGCCTGTCC GCGGAGAGGC ACGCTGGCTG CCCCTGTGTC ACAGCTTCCA TGGATGACAT CTATTTTGAG CACACCATTA GTGTTGGACA AGTGGTGAAT ATCAAGGCCA AGGTGAACCG GGCCTTCAAC TCCAGCATGG AGGTGGGCAT CCAGGTGGCC TCGGAGGACC TGTGCTCTGA GAAGCAGTGG AATGTGTGCA AGGCCTTGGC CACCTTCGTG GCCCGCCGAG AGATCACCAA GGTGAAGCTG AAGCAGATCA CGCCGCGGAC AGAAGAGGAG AAGATGGAGC ACAGTGTGGC GGCTGAGCGC CGGCGCATGC GCCTTGTCTA TGCAGACACC ATCAAGGACC TCCTGGCCAA CTGCGCCATT CAGGGCGATC TGGAGAGCAG AGACTGTAGC CGCATGGTGC CGGCTGAGAA GACCCGTGTG GAGAGTGTGG AGCTGGTCCT GCCTCCCCAC GCCAATCACC AGGGCAACAC CTTTGGGGGC CAGATCATGG CCTGGATGGA GAATGTGGCC ACCATTGCAG CCAGCCGGCT CTGCCGTGCC CACCCTACGC TGAAGGCCAT TGAAATGTTC CACTTCCGAG GCCCGTCCCA GGTCGGCGAC CGTCTGGTGC TCAAAGCCAT CGTGAACAAT GCCTTCAAAC ATAGCATGGA GGTGGGCGTG TGCGTGGAGG CCTATCGCCA GGAGGCTGAG ACCCACCGGC GCCACATCAA CAGTGCCTTT ATGACCTTTG TGGTCCTGGA CGCAGATGAC CAGCCCCAGT TGCTGCCCTG GATTCGGCCC CAGCCCGGCG ATGGTGAGCG GCGGTACCGA GAGGCCAGTG CCAGAAAGAA GATCCGCCTG GACAGGAAGT ACATCGTGTC CTGTAAGCAG ACAGAGGTGC CCCTCTCCGT CCCCTGGGAC CCTAGCAACC AGGTGTACCT GAGCTACAAT AACGTCTCCT CCTTGAAGAT GCTTGTGGCC AAGGACAACT GGGTGCTGTC CTCGGAGATC AGTCAGGTCC GCCTGTACAC TCTGGAGGAT GACAAGTTCC TCTCCTTCCA CATGGAGATG GTGGTGCATG TGGATGCAGC CCAGGCCTTC CTGCTGCTCT CGGACCTGCG TCAGAGGCCA GAGTGGGACA AGCACTACCG GAGCGTGGAG CTAGTGCAGC AGGTAGACGA GGACGACGCC ATCTACCACG TCACCAGCCC TGCCCTCGGA GGTCACACAA AGCCCCAGGA CTTCGTGATC CTGGCCTCGA GGCGGAAGCC TTGTGACAAT GGGGACCCCT ATGTCATCGC GCTGAGGTCG GTCACGCTGC CCACACACCG AGAGACGCCA GAGTACAGAC GCGGAGAGAC CCTCTGCTCA GGCTTCTGCC TCTGGCGCGA GGGGGACCAG CTGACCAAGT GCTGCTGGGT TAGGGTCTCC CTGACTGAGC TGGTCTCGGC AAGTGGCTTC TATTCCTGGG GGCTCGAATC CAGGTCAAAG GGTCGCAGGA GCGACGGTTG GAATGGAAAA CTAGCTGGAG GACACCTGAG TACTCTTAAA GCAATCCCCG TGGCCAAAAT CAACAGCCGA TTTGGATACC TTCAAGACAC CTGAGGATCC ACCGGATCTA GATAACTGAT CATAATTCTA CCGGGTAGGG GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG CAGCCCCGCT GGGCACTTGG CGCTACACAA GTGGCCTCTG GCCTCGCACA CATTCCACAT CCACCGGTAG GCGCCAACCG GCTCCGTTCT TTGGTGGCCC CTTCGCGCCA CCTTCTACTC CTCCCCTAGT CAGGAAGTTC CCCCCCGCCC CGCAGCTCGC GTCGTGCAGG ACGTGACAAA TGGAAGTAGC ACGTCTCACT AGTCTCGTGC AGATGGACAG CACCGCTGAG CAATGGAAGC GGGTAGGCCT TTGGGGCAGC GGCCAATAGC AGCTTTGCTC CTTCGCTTTC TGGGCT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
