pGL4.20-HES1(human)-Promoter-Fluc2-SV40-Puro
$84.00
In stock
SKU
PL58293
100μg liquid plasmid(Instantly):$105
| name | pGL4.20-HES1(human)-Promoter-Fluc2-SV40-Puro | another name | apGL4.20-HES1(human)-Promoter-Fluc2-SV40-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Fluc |
| Promoter | HES1 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | RVP3：CTAGCAAAATAGGCTGTCCC | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCTCCAC CCCGGGTGTG CGCTCCCCGC CGGGAAGAGC TGACCTTGGC CTGGGGAGGC GGGGGTGGGG AGAGAGGGTA AGCGCCGGGG CTCGGCTCCC AGCCCGCCGC CCCCTCGGAT TCCTGGCCCG GCGGGGCCGG AGGAGGGGGC CCCGGACACA CACACACACA CACCCCCCAC ACGCAGCGCG CGCCAATGAG CCCGGGCCAG GCGCAGGGGG CGGGGTTTTC CCAGGCACGA GCACAGCAGC TGCTATTTAC CTTCTTGGCT TCTCCCGGGG ACCAGGAACC GGCCCCCGCT CTCCGCCGCC CGTCGCCGCC CCCCGCCTCC CTGCCCCCGG GGCGCGCGCA CACACACTCA CTCACACACG CACGCACACA CACATCCTCC CGGCCCGGCC GCAGCCGCGG CGGCAATAAA ACATCCTGGC ACGTGCTCCG GCTCCAGGCG CGCCCACGCC GGCCGGCTGA TGTCAAACTG CAGCTCGGCT GGTGTAGCTC TTAAAGGGCC CGCGGGCGCC GGGGGCCGAG GCCGCCCGCG GGGCGAGGAG GGAGAGGCTG CTTCCTTGCC CGCCACCTCA ACGCGCCGCA GAACCTAAAG CCTACGGATG AAAAGGGAAA GGGTGGTGAA GGAGGGCGCG AGTCCCTGAG TCATTGCCCT CGGAGCCCCC CTTGTTCATT TTTTCATTTG AAATACCGTT TCCTTCCACC CAGTTGGAAA TTATTCTGAT TGTTTCCTGG GGAAGCCCGG GGTCTAAGGC CCCAAATCCA AACGAGGAAT TTCTGAAAGA CGGGGTGGGG GTGGGATTCA AGAACTACCT TGCTCCGAAA AACCTGCATT TGTGAGGTAG AAGGCAATTT TTCCTTTTTC TGCATGGAAA CAGGAAAATT TTTTTGGCCC TTTTCCTTTA CCATCTACTT TCACCCTCCT GAATGTAAAG TCTGAGCGGG AACTTTAGAT GTGTCGGTAA CTCACATTCT TACACCCGTC CCCCCCTCCC CCCGCCCCCT TTTAACCACT GCTGTTTTTT TCTTTATTGT TTATACCTTT AAAAAAAATA TGTTTCAAAT GAACTTACTA CAGTCAAAGC AGCTCTGTTA CATATGAGAG AGGGCATAAA GAGCAAAGAC CCTGGCTCCA AAAGAAATAG ACAAGATCAA GACCAAAGCG GAAAGAAAAA AAAAATCTCT AAACCAAAGC CCAGAGGGAG AGTAGCAAAG GGTTAAAATC CTTTTGATTG ACGTTGTAGC CTCCGGTGCC CTGGGCTCAG GCGCGCGCCA TTGGCCGCCA GACCTTGTGC CTGGCGGCCA ATGGGGGGGC GCGGTCCACG AGCGGTGCCG CGTGTCTCCT CCTCCCATTG GCTGAAAGTT ACTGTGGGAA AGAAAGTTTG GGAAGTTTCA CACGAGCCGT TCGCGTGCAG TCCCAGATAT ATATAGAGGC CGCCAGGGCC TAGGGATCAC ACAGGATCCG GAGCTGGTGC TGATAACAGC GGAATCCCCC GTCTACCTCT CTCCTTGGTC CTGGAACAGC GCTACTGATC ACCAAGTAGC CACAAAATAT AATAAACCCT CAGCACTTGC TCAGTAGTTT TGTGAAAGTC TCAAGTAAAA GAGACACAAA CAAAAAATTC TTTTTCGTGA AGAACTCCAA AAATAAAATT CTCTAGAGAT AAAAAAAAAA AAAAAAGGAA AATCTCGAGG ATATCAAGAT CTGGCCTCGG CGGCCAAGCT TGGCAATCCG GTACTGTTGG TAAAGCCACC ATGGAAGATG CCAAAAACAT TAAGAAGGGC CCAGCGCCAT TCTACCCACT CGAAGACGGG ACCGCCGGCG AGCAGCTGCA CAAAGCCATG AAGCGCTACG CCCTGGTGCC CGGCACCATC GCCTTTACCG ACGCACATAT CGAGGTGGAC ATTACCTACG CCGAGTACTT CGAGATGAGC GTTCGGCTGG CAGAAGCTAT GAAGCGCTAT GGGCTGAATA CAAACCATCG GATCGTGGTG TGCAGCGAGA ATAGCTTGCA GTTCTTCATG CCCGTGTTGG GTGCCCTGTT CATCGGTGTG GCTGTGGCCC CAGCTAACGA CATCTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATC AGCCAGCCCA CCGTCGTATT CGTGAGCAAG AAAGGGCTGC AAAAGATCCT CAACGTGCAA AAGAAGCTAC CGATCATACA AAAGATCATC ATCATGGATA GCAAGACCGA CTACCAGGGC TTCCAAAGCA TGTACACCTT CGTGACTTCC CATTTGCCAC CCGGCTTCAA CGAGTACGAC TTCGTGCCCG AGAGCTTCGA CCGGGACAAA ACCATCGCCC TGATCATGAA CAGTAGTGGC AGTACCGGAT TGCCCAAGGG CGTAGCCCTA CCGCACCGCA CCGCTTGTGT CCGATTCAGT CATGCCCGCG ACCCCATCTT CGGCAACCAG ATCATCCCCG ACACCGCTAT CCTCAGCGTG GTGCCATTTC ACCACGGCTT CGGCATGTTC ACCACGCTGG GCTACTTGAT CTGCGGCTTT CGGGTCGTGC TCATGTACCG CTTCGAGGAG GAGCTATTCT TGCGCAGCTT GCAAGACTAT AAGATTCAAT CTGCCCTGCT GGTGCCCACA CTATTTAGCT TCTTCGCTAA GAGCACTCTC ATCGACAAGT ACGACCTAAG CAACTTGCAC GAGATCGCCA GCGGCGGGGC GCCGCTCAGC AAGGAGGTAG GTGAGGCCGT GGCCAAACGC TTCCACCTAC CAGGCATCCG CCAGGGCTAC GGCCTGACAG AAACAACCAG CGCCATTCTG ATCACCCCCG AAGGGGACGA CAAGCCTGGC GCAGTAGGCA AGGTGGTGCC CTTCTTCGAG GCTAAGGTGG TGGACTTGGA CACCGGTAAG ACACTGGGTG TGAACCAGCG CGGCGAGCTG TGCGTCCGTG GCCCCATGAT CATGAGCGGC TACGTTAACA ACCCCGAGGC TACAAACGCT CTCATCGACA AGGACGGCTG GCTGCACAGC GGCGACATCG CCTACTGGGA CGAGGACGAG CACTTCTTCA TCGTGGACCG GCTGAAGAGC CTGATCAAAT ACAAGGGCTA CCAGGTAGCC CCAGCCGAAC TGGAGAGCAT CCTGCTGCAA CACCCCAACA TCTTCGACGC CGGGGTCGCC GGCCTGCCCG ACGACGATGC CGGCGAGCTG CCCGCCGCAG TCGTCGTGCT GGAACACGGT AAAACCATGA CCGAGAAGGA GATCGTGGAC TATGTGGCCA GCCAGGTTAC AACCGCCAAG AAGCTGCGCG GTGGTGTTGT GTTCGTGGAC GAGGTGCCTA AAGGACTGAC CGGCAAGTTG GACGCCCGCA AGATCCGCGA GATTCTCATT AAGGCCAAGA AGGGCGGCAA GATCGCCGTG TAATAATTCT AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGATAAG GATCCGTTTG CGTATTGGGC GCTCTTCCGC TGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGCTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCGATTC TTCTGACACT AGCGCCACCA TGACCGAGTA CAAGCCTACC GTGCGCCTGG CCACTCGCGA TGATGTGCCC CGCGCCGTCC GCACTCTGGC CGCCGCTTTC GCCGACTACC CCGCTACCCG GCACACCGTG GACCCCGACC GGCACATCGA GCGTGTGACA GAGTTGCAGG AGCTGTTCCT GACCCGCGTC GGGCTGGACA TCGGCAAGGT GTGGGTAGCC GACGACGGCG CGGCCGTGGC CGTGTGGACT ACCCCCGAGA GCGTTGAGGC CGGCGCCGTG TTCGCCGAGA TCGGCCCCCG AATGGCCGAG CTGAGCGGCA GCCGCCTGGC CGCCCAGCAG CAAATGGAGG GCCTGCTTGC CCCCCATCGT CCCAAGGAGC CTGCCTGGTT TCTGGCCACT GTAGGAGTGA GCCCCGACCA CCAGGGCAAG GGCTTGGGCA GCGCCGTCGT GTTGCCCGGC GTAGAGGCCG CCGAACGCGC CGGTGTGCCC GCCTTTCTCG AAACAAGCGC ACCAAGAAAC CTTCCATTCT ACGAGCGCCT GGGCTTCACC GTGACCGCCG ATGTCGAGGT GCCCGAGGGA CCTAGGACCT GGTGTATGAC ACGAAAACCT GGCGCCTAAT GATCTAGAAC CGGTCATGGC CGCAATAAAA TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGTTCGAACT AGATGCTGTC GACCGATGCC CTTGAGAGCC TTCAACCCAG TCAGCTCCTT CCGGTGGGCG CGGGGCATGA CTATCGTCGC CGCACTTATG ACTGTCTTCT TTATCATGCA ACTCGTAGGA CAGGTGCCGG CAGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGCGG CCGCAAATGC TAAACCACTG CAGTGGTTAC CAGTGCTTGA TCAGTGAGGC ACCGATCTCA GCGATCTGCC TATTTCGTTC GTCCATAGTG GCCTGACTCC CCGTCGTGTA GATCACTACG ATTCGTGAGG GCTTACCATC AGGCCCCAGC GCAGCAATGA TGCCGCGAGA GCCGCGTTCA CCGGCCCCCG ATTTGTCAGC AATGAACCAG CCAGCAGGGA GGGCCGAGCG AAGAAGTGGT CCTGCTACTT TGTCCGCCTC CATCCAGTCT ATGAGCTGCT GTCGTGATGC TAGAGTAAGA AGTTCGCCAG TGAGTAGTTT CCGAAGAGTT GTGGCCATTG CTACTGGCAT CGTGGTATCA CGCTCGTCGT TCGGTATGGC TTCGTTCAAC TCTGGTTCCC AGCGGTCAAG CCGGGTCACA TGATCACCCA TATTATGAAG AAATGCAGTC AGCTCCTTAG GGCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCGGTGTT GTCGCTCATG GTAATGGCAG CACTACACAA TTCTCTTACC GTCATGCCAT CCGTAAGATG CTTTTCCGTG ACCGGCGAGT ACTCAACCAA GTCGTTTTGT GAGTAGTGTA TACGGCGACC AAGCTGCTCT TGCCCGGCGT CTATACGGGA CAACACCGCG CCACATAGCA GTACTTTGAA AGTGCTCATC ATCGGGAATC GTTCTTCGGG GCGGAAAGAC TCAAGGATCT TGCCGCTATT GAGATCCAGT TCGATATAGC CCACTCTTGC ACCCAGTTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCGGGGTGTG CAAAAACAGG CAAGCAAAAT GCCGCAAAGA AGGGAATGAG TGCGACACGA AAATGTTGGA TGCTCATACT CGTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAC TAGTACGTCT CTCAAGGATA AGTAAGTAAT ATTAAGGTAC GGGAGGTATT GGACAGGCCG CAATAAAATA TCTTTATTTT CATTACATCT GTGTGTTGGT TTTTTGTGTG AATCGATAGT ACTAACATAC GCTCTCCATC AAAACAAAAC GAAACAAAAC AAACTAGCAA AATAGGCTGT CCCCAGTGCA AGTGCAGGTG CCAGAACATT TCTCT
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